Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS09025

Experiment: Bas22

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS09020 and ECOLIN_RS09025 overlap by 4 nucleotidesECOLIN_RS09025 and ECOLIN_RS09030 are separated by 389 nucleotides ECOLIN_RS09020: ECOLIN_RS09020 - glucuronide transporter, at 1,781,461 to 1,782,834 _RS09020 ECOLIN_RS09025: ECOLIN_RS09025 - beta-glucuronidase, at 1,782,831 to 1,784,642 _RS09025 ECOLIN_RS09030: ECOLIN_RS09030 - DNA-binding transcriptional regulator UidR, at 1,785,032 to 1,785,622 _RS09030 Position (kb) 1782 1783 1784 1785Strain fitness (log2 ratio) -1 0 1 2at 1781.912 kb on + strand, within ECOLIN_RS09020at 1782.497 kb on + strand, within ECOLIN_RS09020at 1784.642 kb on + strandat 1784.895 kb on + strandat 1784.909 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas22
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1,781,912 + ECOLIN_RS09020 0.33 +2.1
1,782,497 + ECOLIN_RS09020 0.75 +0.8
1,784,642 + -0.8
1,784,895 + +0.8
1,784,909 - +0.1

Or see this region's nucleotide sequence