Experiment: Bas22
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS00355 and ECOLIN_RS00360 are separated by 113 nucleotides ECOLIN_RS00360 and ECOLIN_RS00365 overlap by 17 nucleotides ECOLIN_RS00365 and ECOLIN_RS00370 overlap by 25 nucleotides
ECOLIN_RS00355: ECOLIN_RS00355 - DedA family protein, at 74,331 to 75,095
_RS00355
ECOLIN_RS00360: ECOLIN_RS00360 - thiamine ABC transporter ATP-binding protein ThiQ, at 75,209 to 75,907
_RS00360
ECOLIN_RS00365: ECOLIN_RS00365 - thiamine/thiamine pyrophosphate ABC transporter permease ThiP, at 75,891 to 77,501
_RS00365
ECOLIN_RS00370: ECOLIN_RS00370 - thiamine ABC transporter substrate binding subunit, at 77,477 to 78,460
_RS00370
Position (kb) 75
76
77
78 Strain fitness (log2 ratio) -1
0
1 at 74.962 kb on - strand, within ECOLIN_RS00355 at 75.060 kb on + strand at 75.060 kb on + strand at 75.169 kb on - strand at 75.208 kb on - strand at 75.211 kb on - strand at 75.211 kb on - strand at 75.603 kb on - strand, within ECOLIN_RS00360 at 75.890 kb on - strand at 76.152 kb on + strand, within ECOLIN_RS00365 at 76.153 kb on - strand, within ECOLIN_RS00365 at 76.474 kb on - strand, within ECOLIN_RS00365 at 76.643 kb on + strand, within ECOLIN_RS00365 at 76.644 kb on - strand, within ECOLIN_RS00365 at 76.810 kb on + strand, within ECOLIN_RS00365 at 76.896 kb on + strand, within ECOLIN_RS00365 at 76.896 kb on + strand, within ECOLIN_RS00365 at 77.028 kb on + strand, within ECOLIN_RS00365 at 77.769 kb on + strand, within ECOLIN_RS00370 at 77.774 kb on - strand, within ECOLIN_RS00370 at 77.818 kb on - strand, within ECOLIN_RS00370 at 77.978 kb on - strand, within ECOLIN_RS00370 at 78.381 kb on + strand at 78.381 kb on + strand at 78.382 kb on - strand at 78.382 kb on - strand at 78.385 kb on + strand at 78.386 kb on - strand at 78.387 kb on + strand at 78.388 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas22 remove 74,962 - ECOLIN_RS00355 0.82 +0.3 75,060 + -1.2 75,060 + -0.3 75,169 - +0.4 75,208 - -0.4 75,211 - -0.4 75,211 - +0.4 75,603 - ECOLIN_RS00360 0.56 -1.0 75,890 - +0.3 76,152 + ECOLIN_RS00365 0.16 -0.4 76,153 - ECOLIN_RS00365 0.16 +0.0 76,474 - ECOLIN_RS00365 0.36 +0.1 76,643 + ECOLIN_RS00365 0.47 -0.4 76,644 - ECOLIN_RS00365 0.47 -0.3 76,810 + ECOLIN_RS00365 0.57 -1.2 76,896 + ECOLIN_RS00365 0.62 -0.1 76,896 + ECOLIN_RS00365 0.62 -0.3 77,028 + ECOLIN_RS00365 0.71 +1.6 77,769 + ECOLIN_RS00370 0.30 -0.0 77,774 - ECOLIN_RS00370 0.30 -0.2 77,818 - ECOLIN_RS00370 0.35 -0.3 77,978 - ECOLIN_RS00370 0.51 -0.2 78,381 + -0.2 78,381 + +0.9 78,382 - +0.1 78,382 - +0.2 78,385 + -0.6 78,386 - +0.4 78,387 + +0.1 78,388 - -0.2
Or see this region's nucleotide sequence