Experiment: D-Raffinose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS10255 and HMPREF1078_RS10260 are separated by 46 nucleotides HMPREF1078_RS10260 and HMPREF1078_RS10265 are separated by 85 nucleotides HMPREF1078_RS10265 and HMPREF1078_RS10270 are separated by 49 nucleotides HMPREF1078_RS10270 and HMPREF1078_RS10275 are separated by 83 nucleotides
HMPREF1078_RS10255: HMPREF1078_RS10255 - NAD kinase, at 679,389 to 680,267
_RS10255
HMPREF1078_RS10260: HMPREF1078_RS10260 - CBS domain-containing protein, at 680,314 to 680,973
_RS10260
HMPREF1078_RS10265: HMPREF1078_RS10265 - pyridoxine 5'-phosphate synthase, at 681,059 to 681,775
_RS10265
HMPREF1078_RS10270: HMPREF1078_RS10270 - MotA/TolQ/ExbB proton channel family protein, at 681,825 to 682,535
_RS10270
HMPREF1078_RS10275: HMPREF1078_RS10275 - biopolymer transporter ExbD, at 682,619 to 683,029
_RS10275
Position (kb) 681
682 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 680.287 kb on - strand at 680.298 kb on - strand at 680.318 kb on - strand at 680.318 kb on - strand at 680.318 kb on - strand at 680.318 kb on - strand at 680.328 kb on - strand at 680.328 kb on - strand at 680.391 kb on - strand, within HMPREF1078_RS10260 at 680.404 kb on - strand, within HMPREF1078_RS10260 at 680.404 kb on - strand, within HMPREF1078_RS10260 at 680.404 kb on - strand, within HMPREF1078_RS10260 at 680.406 kb on - strand, within HMPREF1078_RS10260 at 680.406 kb on - strand, within HMPREF1078_RS10260 at 680.670 kb on - strand, within HMPREF1078_RS10260 at 680.683 kb on - strand, within HMPREF1078_RS10260 at 680.687 kb on - strand, within HMPREF1078_RS10260 at 680.739 kb on - strand, within HMPREF1078_RS10260 at 680.739 kb on - strand, within HMPREF1078_RS10260 at 680.749 kb on - strand, within HMPREF1078_RS10260 at 680.794 kb on - strand, within HMPREF1078_RS10260 at 680.847 kb on - strand, within HMPREF1078_RS10260 at 680.849 kb on - strand, within HMPREF1078_RS10260 at 680.849 kb on - strand, within HMPREF1078_RS10260 at 680.910 kb on - strand at 680.982 kb on - strand at 680.982 kb on - strand at 680.982 kb on - strand at 680.995 kb on - strand at 681.001 kb on - strand at 681.069 kb on + strand at 681.082 kb on + strand at 681.124 kb on - strand at 681.132 kb on + strand, within HMPREF1078_RS10265 at 681.132 kb on + strand, within HMPREF1078_RS10265 at 681.133 kb on - strand, within HMPREF1078_RS10265 at 681.141 kb on + strand, within HMPREF1078_RS10265 at 681.147 kb on + strand, within HMPREF1078_RS10265 at 681.178 kb on + strand, within HMPREF1078_RS10265 at 681.228 kb on - strand, within HMPREF1078_RS10265 at 681.228 kb on - strand, within HMPREF1078_RS10265 at 681.228 kb on - strand, within HMPREF1078_RS10265 at 681.228 kb on - strand, within HMPREF1078_RS10265 at 681.228 kb on - strand, within HMPREF1078_RS10265 at 681.260 kb on - strand, within HMPREF1078_RS10265 at 681.264 kb on + strand, within HMPREF1078_RS10265 at 681.265 kb on - strand, within HMPREF1078_RS10265 at 681.265 kb on - strand, within HMPREF1078_RS10265 at 681.290 kb on - strand, within HMPREF1078_RS10265 at 681.291 kb on + strand, within HMPREF1078_RS10265 at 681.291 kb on + strand, within HMPREF1078_RS10265 at 681.291 kb on + strand, within HMPREF1078_RS10265 at 681.439 kb on + strand, within HMPREF1078_RS10265 at 681.439 kb on + strand, within HMPREF1078_RS10265 at 681.439 kb on + strand, within HMPREF1078_RS10265 at 681.440 kb on - strand, within HMPREF1078_RS10265 at 681.440 kb on - strand, within HMPREF1078_RS10265 at 681.440 kb on - strand, within HMPREF1078_RS10265 at 681.515 kb on + strand, within HMPREF1078_RS10265 at 681.516 kb on - strand, within HMPREF1078_RS10265 at 681.518 kb on + strand, within HMPREF1078_RS10265 at 681.527 kb on + strand, within HMPREF1078_RS10265 at 681.528 kb on - strand, within HMPREF1078_RS10265 at 681.528 kb on - strand, within HMPREF1078_RS10265 at 681.539 kb on + strand, within HMPREF1078_RS10265 at 681.546 kb on + strand, within HMPREF1078_RS10265 at 681.615 kb on + strand, within HMPREF1078_RS10265 at 681.616 kb on - strand, within HMPREF1078_RS10265 at 681.654 kb on - strand, within HMPREF1078_RS10265 at 681.723 kb on - strand at 681.725 kb on + strand at 681.753 kb on - strand at 681.753 kb on - strand at 681.754 kb on + strand at 681.770 kb on + strand at 681.787 kb on + strand at 681.794 kb on + strand at 681.795 kb on - strand at 681.795 kb on - strand at 681.824 kb on + strand at 681.836 kb on - strand at 681.925 kb on + strand, within HMPREF1078_RS10270 at 681.925 kb on + strand, within HMPREF1078_RS10270 at 681.941 kb on + strand, within HMPREF1078_RS10270 at 681.967 kb on + strand, within HMPREF1078_RS10270 at 681.974 kb on - strand, within HMPREF1078_RS10270 at 681.974 kb on - strand, within HMPREF1078_RS10270 at 681.993 kb on + strand, within HMPREF1078_RS10270 at 681.994 kb on - strand, within HMPREF1078_RS10270 at 681.995 kb on + strand, within HMPREF1078_RS10270 at 681.996 kb on - strand, within HMPREF1078_RS10270 at 681.996 kb on - strand, within HMPREF1078_RS10270 at 681.997 kb on + strand, within HMPREF1078_RS10270 at 681.997 kb on + strand, within HMPREF1078_RS10270 at 682.154 kb on + strand, within HMPREF1078_RS10270 at 682.154 kb on + strand, within HMPREF1078_RS10270 at 682.155 kb on - strand, within HMPREF1078_RS10270 at 682.291 kb on + strand, within HMPREF1078_RS10270 at 682.292 kb on - strand, within HMPREF1078_RS10270 at 682.295 kb on + strand, within HMPREF1078_RS10270 at 682.335 kb on - strand, within HMPREF1078_RS10270 at 682.351 kb on + strand, within HMPREF1078_RS10270 at 682.357 kb on + strand, within HMPREF1078_RS10270 at 682.358 kb on - strand, within HMPREF1078_RS10270 at 682.358 kb on - strand, within HMPREF1078_RS10270 at 682.376 kb on + strand, within HMPREF1078_RS10270 at 682.446 kb on + strand, within HMPREF1078_RS10270 at 682.446 kb on + strand, within HMPREF1078_RS10270 at 682.447 kb on - strand, within HMPREF1078_RS10270 at 682.447 kb on - strand, within HMPREF1078_RS10270 at 682.505 kb on + strand at 682.563 kb on - strand at 682.576 kb on + strand at 682.577 kb on - strand at 682.577 kb on - strand at 682.577 kb on - strand at 682.577 kb on - strand at 682.584 kb on + strand at 682.584 kb on + strand at 682.585 kb on - strand at 682.585 kb on - strand at 682.598 kb on + strand at 682.605 kb on + strand at 682.684 kb on - strand, within HMPREF1078_RS10275 at 682.706 kb on - strand, within HMPREF1078_RS10275 at 682.712 kb on - strand, within HMPREF1078_RS10275
Per-strain Table
Position Strand Gene LocusTag Fraction D-Raffinose 10 mg/mL (C) remove 680,287 - +0.0 680,298 - -0.7 680,318 - +0.4 680,318 - -1.4 680,318 - +0.5 680,318 - +1.1 680,328 - -1.6 680,328 - +1.1 680,391 - HMPREF1078_RS10260 0.12 -0.6 680,404 - HMPREF1078_RS10260 0.14 +0.8 680,404 - HMPREF1078_RS10260 0.14 -0.4 680,404 - HMPREF1078_RS10260 0.14 -0.4 680,406 - HMPREF1078_RS10260 0.14 -0.7 680,406 - HMPREF1078_RS10260 0.14 -1.0 680,670 - HMPREF1078_RS10260 0.54 +0.3 680,683 - HMPREF1078_RS10260 0.56 +0.3 680,687 - HMPREF1078_RS10260 0.57 -2.2 680,739 - HMPREF1078_RS10260 0.64 +0.1 680,739 - HMPREF1078_RS10260 0.64 +0.5 680,749 - HMPREF1078_RS10260 0.66 -1.0 680,794 - HMPREF1078_RS10260 0.73 +0.3 680,847 - HMPREF1078_RS10260 0.81 +1.1 680,849 - HMPREF1078_RS10260 0.81 +0.1 680,849 - HMPREF1078_RS10260 0.81 +0.8 680,910 - -1.2 680,982 - -0.5 680,982 - -1.0 680,982 - -0.7 680,995 - -3.1 681,001 - -1.6 681,069 + -1.9 681,082 + -0.7 681,124 - -0.6 681,132 + HMPREF1078_RS10265 0.10 -0.2 681,132 + HMPREF1078_RS10265 0.10 -2.7 681,133 - HMPREF1078_RS10265 0.10 +0.1 681,141 + HMPREF1078_RS10265 0.11 -3.4 681,147 + HMPREF1078_RS10265 0.12 -1.2 681,178 + HMPREF1078_RS10265 0.17 -1.3 681,228 - HMPREF1078_RS10265 0.24 -1.5 681,228 - HMPREF1078_RS10265 0.24 -0.4 681,228 - HMPREF1078_RS10265 0.24 -1.0 681,228 - HMPREF1078_RS10265 0.24 +0.4 681,228 - HMPREF1078_RS10265 0.24 -1.4 681,260 - HMPREF1078_RS10265 0.28 -2.2 681,264 + HMPREF1078_RS10265 0.29 -0.5 681,265 - HMPREF1078_RS10265 0.29 -1.2 681,265 - HMPREF1078_RS10265 0.29 -0.7 681,290 - HMPREF1078_RS10265 0.32 -2.2 681,291 + HMPREF1078_RS10265 0.32 -0.5 681,291 + HMPREF1078_RS10265 0.32 -2.1 681,291 + HMPREF1078_RS10265 0.32 -1.7 681,439 + HMPREF1078_RS10265 0.53 -0.7 681,439 + HMPREF1078_RS10265 0.53 -2.4 681,439 + HMPREF1078_RS10265 0.53 +0.1 681,440 - HMPREF1078_RS10265 0.53 +0.4 681,440 - HMPREF1078_RS10265 0.53 -0.7 681,440 - HMPREF1078_RS10265 0.53 -0.7 681,515 + HMPREF1078_RS10265 0.64 -0.2 681,516 - HMPREF1078_RS10265 0.64 -2.3 681,518 + HMPREF1078_RS10265 0.64 -0.8 681,527 + HMPREF1078_RS10265 0.65 +0.4 681,528 - HMPREF1078_RS10265 0.65 +0.1 681,528 - HMPREF1078_RS10265 0.65 +0.4 681,539 + HMPREF1078_RS10265 0.67 -0.1 681,546 + HMPREF1078_RS10265 0.68 +1.6 681,615 + HMPREF1078_RS10265 0.78 +0.3 681,616 - HMPREF1078_RS10265 0.78 +0.1 681,654 - HMPREF1078_RS10265 0.83 -0.4 681,723 - -1.7 681,725 + +0.4 681,753 - -2.0 681,753 - -2.0 681,754 + -1.4 681,770 + +0.4 681,787 + -0.1 681,794 + -1.0 681,795 - -0.2 681,795 - -1.5 681,824 + +0.1 681,836 - -0.6 681,925 + HMPREF1078_RS10270 0.14 -0.9 681,925 + HMPREF1078_RS10270 0.14 -1.3 681,941 + HMPREF1078_RS10270 0.16 -1.0 681,967 + HMPREF1078_RS10270 0.20 -1.7 681,974 - HMPREF1078_RS10270 0.21 -2.6 681,974 - HMPREF1078_RS10270 0.21 -2.4 681,993 + HMPREF1078_RS10270 0.24 +0.1 681,994 - HMPREF1078_RS10270 0.24 -2.5 681,995 + HMPREF1078_RS10270 0.24 -1.6 681,996 - HMPREF1078_RS10270 0.24 +0.4 681,996 - HMPREF1078_RS10270 0.24 -2.0 681,997 + HMPREF1078_RS10270 0.24 -2.7 681,997 + HMPREF1078_RS10270 0.24 -1.4 682,154 + HMPREF1078_RS10270 0.46 +0.1 682,154 + HMPREF1078_RS10270 0.46 +0.1 682,155 - HMPREF1078_RS10270 0.46 -3.2 682,291 + HMPREF1078_RS10270 0.66 -0.7 682,292 - HMPREF1078_RS10270 0.66 -2.0 682,295 + HMPREF1078_RS10270 0.66 -0.4 682,335 - HMPREF1078_RS10270 0.72 -1.5 682,351 + HMPREF1078_RS10270 0.74 -0.7 682,357 + HMPREF1078_RS10270 0.75 -1.0 682,358 - HMPREF1078_RS10270 0.75 -1.8 682,358 - HMPREF1078_RS10270 0.75 -2.3 682,376 + HMPREF1078_RS10270 0.77 -1.4 682,446 + HMPREF1078_RS10270 0.87 -1.2 682,446 + HMPREF1078_RS10270 0.87 -0.2 682,447 - HMPREF1078_RS10270 0.87 -0.2 682,447 - HMPREF1078_RS10270 0.87 +0.1 682,505 + -0.9 682,563 - -1.3 682,576 + -0.1 682,577 - -1.5 682,577 - -0.9 682,577 - -0.6 682,577 - -2.3 682,584 + -2.4 682,584 + -0.2 682,585 - -1.2 682,585 - -2.6 682,598 + -2.8 682,605 + -2.1 682,684 - HMPREF1078_RS10275 0.16 -2.3 682,706 - HMPREF1078_RS10275 0.21 -1.7 682,712 - HMPREF1078_RS10275 0.23 -1.9
Or see this region's nucleotide sequence