Experiment: D-Maltose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS04400 and HMPREF1078_RS04405 are separated by 97 nucleotides HMPREF1078_RS04405 and HMPREF1078_RS04410 are separated by 15 nucleotides HMPREF1078_RS04410 and HMPREF1078_RS04415 are separated by 87 nucleotides HMPREF1078_RS04415 and HMPREF1078_RS04420 are separated by 94 nucleotides
HMPREF1078_RS04400: HMPREF1078_RS04400 - alanine--tRNA ligase, at 109,476 to 112,103
_RS04400
HMPREF1078_RS04405: HMPREF1078_RS04405 - M23 family metallopeptidase, at 112,201 to 113,184
_RS04405
HMPREF1078_RS04410: HMPREF1078_RS04410 - MerR family transcriptional regulator, at 113,200 to 113,556
_RS04410
HMPREF1078_RS04415: HMPREF1078_RS04415 - DUF3127 domain-containing protein, at 113,644 to 114,039
_RS04415
HMPREF1078_RS04420: HMPREF1078_RS04420 - RelA/SpoT family protein, at 114,134 to 116,428
_RS04420
Position (kb)
112
113
114 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 112.228 kb on + strand at 112.235 kb on + strand at 112.235 kb on + strand at 112.235 kb on + strand at 112.237 kb on + strand at 112.237 kb on + strand at 112.237 kb on + strand at 112.237 kb on + strand at 112.237 kb on + strand at 112.350 kb on + strand, within HMPREF1078_RS04405 at 112.423 kb on + strand, within HMPREF1078_RS04405 at 112.423 kb on + strand, within HMPREF1078_RS04405 at 112.475 kb on + strand, within HMPREF1078_RS04405 at 112.475 kb on + strand, within HMPREF1078_RS04405 at 112.651 kb on + strand, within HMPREF1078_RS04405 at 112.719 kb on + strand, within HMPREF1078_RS04405 at 112.761 kb on + strand, within HMPREF1078_RS04405 at 112.761 kb on + strand, within HMPREF1078_RS04405 at 112.803 kb on + strand, within HMPREF1078_RS04405 at 112.858 kb on + strand, within HMPREF1078_RS04405 at 112.906 kb on + strand, within HMPREF1078_RS04405 at 112.954 kb on + strand, within HMPREF1078_RS04405 at 112.997 kb on + strand, within HMPREF1078_RS04405 at 113.086 kb on + strand at 113.104 kb on + strand at 113.107 kb on + strand at 113.110 kb on + strand at 113.110 kb on + strand at 113.142 kb on + strand at 113.142 kb on + strand at 113.142 kb on + strand at 113.561 kb on + strand at 113.562 kb on - strand at 113.562 kb on - strand at 113.596 kb on + strand at 113.611 kb on + strand at 113.630 kb on + strand at 113.728 kb on + strand, within HMPREF1078_RS04415 at 113.728 kb on + strand, within HMPREF1078_RS04415 at 113.728 kb on + strand, within HMPREF1078_RS04415 at 113.729 kb on - strand, within HMPREF1078_RS04415 at 113.729 kb on - strand, within HMPREF1078_RS04415 at 113.729 kb on - strand, within HMPREF1078_RS04415 at 113.729 kb on - strand, within HMPREF1078_RS04415 at 113.729 kb on - strand, within HMPREF1078_RS04415 at 113.729 kb on - strand, within HMPREF1078_RS04415 at 113.729 kb on - strand, within HMPREF1078_RS04415 at 113.765 kb on + strand, within HMPREF1078_RS04415 at 113.765 kb on + strand, within HMPREF1078_RS04415 at 113.765 kb on + strand, within HMPREF1078_RS04415 at 113.765 kb on + strand, within HMPREF1078_RS04415 at 113.810 kb on + strand, within HMPREF1078_RS04415 at 113.829 kb on + strand, within HMPREF1078_RS04415 at 113.829 kb on + strand, within HMPREF1078_RS04415 at 113.829 kb on + strand, within HMPREF1078_RS04415 at 113.830 kb on - strand, within HMPREF1078_RS04415 at 113.830 kb on - strand, within HMPREF1078_RS04415 at 113.830 kb on - strand, within HMPREF1078_RS04415 at 113.834 kb on + strand, within HMPREF1078_RS04415 at 113.846 kb on + strand, within HMPREF1078_RS04415 at 113.861 kb on - strand, within HMPREF1078_RS04415 at 113.883 kb on + strand, within HMPREF1078_RS04415 at 113.884 kb on - strand, within HMPREF1078_RS04415 at 113.960 kb on + strand, within HMPREF1078_RS04415 at 114.038 kb on - strand at 114.097 kb on + strand at 114.099 kb on + strand at 114.106 kb on + strand at 114.135 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Maltose 10 mg/mL (C) remove 112,228 + +1.7 112,235 + -0.5 112,235 + -0.8 112,235 + -0.4 112,237 + -1.3 112,237 + -3.3 112,237 + -0.0 112,237 + -0.5 112,237 + -0.9 112,350 + HMPREF1078_RS04405 0.15 -2.3 112,423 + HMPREF1078_RS04405 0.23 -2.2 112,423 + HMPREF1078_RS04405 0.23 -0.9 112,475 + HMPREF1078_RS04405 0.28 -0.7 112,475 + HMPREF1078_RS04405 0.28 -1.5 112,651 + HMPREF1078_RS04405 0.46 -1.0 112,719 + HMPREF1078_RS04405 0.53 -0.7 112,761 + HMPREF1078_RS04405 0.57 -0.5 112,761 + HMPREF1078_RS04405 0.57 -3.1 112,803 + HMPREF1078_RS04405 0.61 -2.7 112,858 + HMPREF1078_RS04405 0.67 -1.3 112,906 + HMPREF1078_RS04405 0.72 -2.0 112,954 + HMPREF1078_RS04405 0.77 +1.4 112,997 + HMPREF1078_RS04405 0.81 -0.7 113,086 + -1.6 113,104 + -1.5 113,107 + -1.7 113,110 + -1.4 113,110 + -1.2 113,142 + -0.9 113,142 + +1.4 113,142 + -0.1 113,561 + -0.4 113,562 - +2.8 113,562 - -0.2 113,596 + -2.1 113,611 + +1.9 113,630 + -3.3 113,728 + HMPREF1078_RS04415 0.21 -1.3 113,728 + HMPREF1078_RS04415 0.21 -0.9 113,728 + HMPREF1078_RS04415 0.21 -0.0 113,729 - HMPREF1078_RS04415 0.21 -1.3 113,729 - HMPREF1078_RS04415 0.21 -2.6 113,729 - HMPREF1078_RS04415 0.21 +0.7 113,729 - HMPREF1078_RS04415 0.21 +1.3 113,729 - HMPREF1078_RS04415 0.21 -2.3 113,729 - HMPREF1078_RS04415 0.21 -1.4 113,729 - HMPREF1078_RS04415 0.21 -0.9 113,765 + HMPREF1078_RS04415 0.31 -2.2 113,765 + HMPREF1078_RS04415 0.31 -2.0 113,765 + HMPREF1078_RS04415 0.31 -1.7 113,765 + HMPREF1078_RS04415 0.31 -1.7 113,810 + HMPREF1078_RS04415 0.42 -0.5 113,829 + HMPREF1078_RS04415 0.47 -1.5 113,829 + HMPREF1078_RS04415 0.47 -2.7 113,829 + HMPREF1078_RS04415 0.47 -2.1 113,830 - HMPREF1078_RS04415 0.47 +0.6 113,830 - HMPREF1078_RS04415 0.47 -1.2 113,830 - HMPREF1078_RS04415 0.47 -0.2 113,834 + HMPREF1078_RS04415 0.48 -0.6 113,846 + HMPREF1078_RS04415 0.51 -2.7 113,861 - HMPREF1078_RS04415 0.55 -2.1 113,883 + HMPREF1078_RS04415 0.60 -0.7 113,884 - HMPREF1078_RS04415 0.61 -1.2 113,960 + HMPREF1078_RS04415 0.80 -0.3 114,038 - -0.7 114,097 + -2.8 114,099 + +1.1 114,106 + -0.0 114,135 + -0.8
Or see this region's nucleotide sequence