Experiment: Beta-Lactose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS16675 and HMPREF1078_RS16680 are separated by 85 nucleotides HMPREF1078_RS16680 and HMPREF1078_RS16685 are separated by 40 nucleotides HMPREF1078_RS16685 and HMPREF1078_RS16690 are separated by 12 nucleotides
HMPREF1078_RS16675: HMPREF1078_RS16675 - ketol-acid reductoisomerase, at 47,938 to 48,981
_RS16675
HMPREF1078_RS16680: HMPREF1078_RS16680 - thioesterase, at 49,067 to 49,804
_RS16680
HMPREF1078_RS16685: HMPREF1078_RS16685 - acetolactate synthase small subunit, at 49,845 to 50,396
_RS16685
HMPREF1078_RS16690: HMPREF1078_RS16690 - biosynthetic-type acetolactate synthase large subunit, at 50,409 to 52,109
_RS16690
Position (kb)
49
50 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 48.112 kb on + strand, within HMPREF1078_RS16675 at 48.113 kb on - strand, within HMPREF1078_RS16675 at 48.113 kb on - strand, within HMPREF1078_RS16675 at 48.188 kb on + strand, within HMPREF1078_RS16675 at 48.189 kb on - strand, within HMPREF1078_RS16675 at 48.189 kb on - strand, within HMPREF1078_RS16675 at 48.189 kb on - strand, within HMPREF1078_RS16675 at 48.189 kb on - strand, within HMPREF1078_RS16675 at 48.317 kb on - strand, within HMPREF1078_RS16675 at 48.317 kb on - strand, within HMPREF1078_RS16675 at 48.317 kb on - strand, within HMPREF1078_RS16675 at 48.368 kb on - strand, within HMPREF1078_RS16675 at 48.402 kb on - strand, within HMPREF1078_RS16675 at 48.402 kb on - strand, within HMPREF1078_RS16675 at 48.477 kb on - strand, within HMPREF1078_RS16675 at 48.576 kb on + strand, within HMPREF1078_RS16675 at 48.580 kb on - strand, within HMPREF1078_RS16675 at 48.586 kb on - strand, within HMPREF1078_RS16675 at 48.704 kb on - strand, within HMPREF1078_RS16675 at 48.708 kb on + strand, within HMPREF1078_RS16675 at 48.808 kb on + strand, within HMPREF1078_RS16675 at 48.876 kb on + strand, within HMPREF1078_RS16675 at 48.902 kb on - strand at 48.988 kb on + strand at 48.994 kb on + strand at 48.994 kb on + strand at 49.000 kb on + strand at 49.008 kb on + strand at 49.096 kb on + strand at 49.111 kb on + strand at 49.111 kb on + strand at 49.156 kb on - strand, within HMPREF1078_RS16680 at 49.194 kb on + strand, within HMPREF1078_RS16680 at 49.216 kb on + strand, within HMPREF1078_RS16680 at 49.266 kb on + strand, within HMPREF1078_RS16680 at 49.268 kb on - strand, within HMPREF1078_RS16680 at 49.268 kb on - strand, within HMPREF1078_RS16680 at 49.269 kb on + strand, within HMPREF1078_RS16680 at 49.276 kb on + strand, within HMPREF1078_RS16680 at 49.277 kb on - strand, within HMPREF1078_RS16680 at 49.277 kb on - strand, within HMPREF1078_RS16680 at 49.277 kb on - strand, within HMPREF1078_RS16680 at 49.311 kb on + strand, within HMPREF1078_RS16680 at 49.324 kb on - strand, within HMPREF1078_RS16680 at 49.324 kb on - strand, within HMPREF1078_RS16680 at 49.401 kb on + strand, within HMPREF1078_RS16680 at 49.401 kb on + strand, within HMPREF1078_RS16680 at 49.401 kb on + strand, within HMPREF1078_RS16680 at 49.402 kb on - strand, within HMPREF1078_RS16680 at 49.468 kb on + strand, within HMPREF1078_RS16680 at 49.468 kb on + strand, within HMPREF1078_RS16680 at 49.492 kb on + strand, within HMPREF1078_RS16680 at 49.561 kb on - strand, within HMPREF1078_RS16680 at 49.570 kb on + strand, within HMPREF1078_RS16680 at 49.571 kb on + strand, within HMPREF1078_RS16680 at 49.571 kb on - strand, within HMPREF1078_RS16680 at 49.571 kb on - strand, within HMPREF1078_RS16680 at 49.571 kb on - strand, within HMPREF1078_RS16680 at 49.634 kb on - strand, within HMPREF1078_RS16680 at 49.648 kb on + strand, within HMPREF1078_RS16680 at 49.649 kb on - strand, within HMPREF1078_RS16680 at 49.651 kb on - strand, within HMPREF1078_RS16680 at 49.697 kb on + strand, within HMPREF1078_RS16680 at 49.697 kb on + strand, within HMPREF1078_RS16680 at 49.697 kb on + strand, within HMPREF1078_RS16680 at 49.698 kb on - strand, within HMPREF1078_RS16680 at 49.705 kb on - strand, within HMPREF1078_RS16680 at 49.728 kb on - strand, within HMPREF1078_RS16680 at 49.841 kb on + strand at 49.865 kb on + strand at 49.866 kb on - strand at 49.866 kb on - strand at 49.887 kb on - strand at 50.000 kb on - strand, within HMPREF1078_RS16685 at 50.058 kb on - strand, within HMPREF1078_RS16685 at 50.058 kb on - strand, within HMPREF1078_RS16685 at 50.060 kb on - strand, within HMPREF1078_RS16685 at 50.118 kb on - strand, within HMPREF1078_RS16685 at 50.119 kb on + strand, within HMPREF1078_RS16685 at 50.119 kb on + strand, within HMPREF1078_RS16685 at 50.156 kb on + strand, within HMPREF1078_RS16685 at 50.161 kb on + strand, within HMPREF1078_RS16685 at 50.162 kb on - strand, within HMPREF1078_RS16685 at 50.165 kb on - strand, within HMPREF1078_RS16685 at 50.178 kb on - strand, within HMPREF1078_RS16685 at 50.253 kb on - strand, within HMPREF1078_RS16685 at 50.256 kb on + strand, within HMPREF1078_RS16685 at 50.257 kb on - strand, within HMPREF1078_RS16685 at 50.257 kb on - strand, within HMPREF1078_RS16685 at 50.257 kb on - strand, within HMPREF1078_RS16685 at 50.258 kb on + strand, within HMPREF1078_RS16685 at 50.346 kb on + strand at 50.348 kb on - strand at 50.351 kb on + strand at 50.376 kb on - strand at 50.376 kb on - strand at 50.376 kb on - strand at 50.396 kb on + strand at 50.396 kb on + strand at 50.414 kb on + strand at 50.415 kb on - strand at 50.424 kb on + strand at 50.424 kb on + strand at 50.425 kb on - strand at 50.436 kb on + strand at 50.466 kb on + strand at 50.467 kb on - strand at 50.467 kb on - strand at 50.467 kb on - strand at 50.471 kb on + strand at 50.471 kb on + strand at 50.472 kb on - strand at 50.627 kb on + strand, within HMPREF1078_RS16690 at 50.628 kb on - strand, within HMPREF1078_RS16690 at 50.628 kb on - strand, within HMPREF1078_RS16690 at 50.628 kb on - strand, within HMPREF1078_RS16690 at 50.687 kb on + strand, within HMPREF1078_RS16690 at 50.688 kb on - strand, within HMPREF1078_RS16690 at 50.793 kb on - strand, within HMPREF1078_RS16690
Per-strain Table
Position Strand Gene LocusTag Fraction Beta-Lactose 10 mg/mL (C) remove 48,112 + HMPREF1078_RS16675 0.17 -3.5 48,113 - HMPREF1078_RS16675 0.17 -3.3 48,113 - HMPREF1078_RS16675 0.17 -4.7 48,188 + HMPREF1078_RS16675 0.24 -3.8 48,189 - HMPREF1078_RS16675 0.24 -4.8 48,189 - HMPREF1078_RS16675 0.24 -3.6 48,189 - HMPREF1078_RS16675 0.24 -4.0 48,189 - HMPREF1078_RS16675 0.24 -4.4 48,317 - HMPREF1078_RS16675 0.36 -2.4 48,317 - HMPREF1078_RS16675 0.36 -5.2 48,317 - HMPREF1078_RS16675 0.36 -3.2 48,368 - HMPREF1078_RS16675 0.41 -2.1 48,402 - HMPREF1078_RS16675 0.44 -3.3 48,402 - HMPREF1078_RS16675 0.44 -3.4 48,477 - HMPREF1078_RS16675 0.52 -4.7 48,576 + HMPREF1078_RS16675 0.61 -2.3 48,580 - HMPREF1078_RS16675 0.61 -4.0 48,586 - HMPREF1078_RS16675 0.62 -4.3 48,704 - HMPREF1078_RS16675 0.73 -3.3 48,708 + HMPREF1078_RS16675 0.74 +0.5 48,808 + HMPREF1078_RS16675 0.83 -2.7 48,876 + HMPREF1078_RS16675 0.90 -4.6 48,902 - -4.3 48,988 + -4.1 48,994 + -1.2 48,994 + -2.6 49,000 + -1.2 49,008 + -3.1 49,096 + -5.1 49,111 + -2.3 49,111 + -3.0 49,156 - HMPREF1078_RS16680 0.12 +0.3 49,194 + HMPREF1078_RS16680 0.17 -2.9 49,216 + HMPREF1078_RS16680 0.20 -1.7 49,266 + HMPREF1078_RS16680 0.27 -1.5 49,268 - HMPREF1078_RS16680 0.27 +1.4 49,268 - HMPREF1078_RS16680 0.27 -0.4 49,269 + HMPREF1078_RS16680 0.27 -2.3 49,276 + HMPREF1078_RS16680 0.28 -5.1 49,277 - HMPREF1078_RS16680 0.28 -0.9 49,277 - HMPREF1078_RS16680 0.28 +0.9 49,277 - HMPREF1078_RS16680 0.28 -0.4 49,311 + HMPREF1078_RS16680 0.33 -3.2 49,324 - HMPREF1078_RS16680 0.35 +1.2 49,324 - HMPREF1078_RS16680 0.35 +0.4 49,401 + HMPREF1078_RS16680 0.45 -5.3 49,401 + HMPREF1078_RS16680 0.45 -3.8 49,401 + HMPREF1078_RS16680 0.45 -2.5 49,402 - HMPREF1078_RS16680 0.45 +1.0 49,468 + HMPREF1078_RS16680 0.54 -1.1 49,468 + HMPREF1078_RS16680 0.54 -3.6 49,492 + HMPREF1078_RS16680 0.58 -5.0 49,561 - HMPREF1078_RS16680 0.67 +0.2 49,570 + HMPREF1078_RS16680 0.68 -1.5 49,571 + HMPREF1078_RS16680 0.68 -3.0 49,571 - HMPREF1078_RS16680 0.68 -0.8 49,571 - HMPREF1078_RS16680 0.68 -0.8 49,571 - HMPREF1078_RS16680 0.68 -0.1 49,634 - HMPREF1078_RS16680 0.77 -0.1 49,648 + HMPREF1078_RS16680 0.79 -3.6 49,649 - HMPREF1078_RS16680 0.79 -1.6 49,651 - HMPREF1078_RS16680 0.79 -1.0 49,697 + HMPREF1078_RS16680 0.85 -1.8 49,697 + HMPREF1078_RS16680 0.85 -2.3 49,697 + HMPREF1078_RS16680 0.85 -3.3 49,698 - HMPREF1078_RS16680 0.86 -0.7 49,705 - HMPREF1078_RS16680 0.86 -1.9 49,728 - HMPREF1078_RS16680 0.90 -0.3 49,841 + -3.5 49,865 + -3.6 49,866 - -1.6 49,866 - +0.2 49,887 - -1.7 50,000 - HMPREF1078_RS16685 0.28 -3.8 50,058 - HMPREF1078_RS16685 0.39 -2.2 50,058 - HMPREF1078_RS16685 0.39 -1.4 50,060 - HMPREF1078_RS16685 0.39 -0.3 50,118 - HMPREF1078_RS16685 0.49 -1.3 50,119 + HMPREF1078_RS16685 0.50 -3.2 50,119 + HMPREF1078_RS16685 0.50 -0.3 50,156 + HMPREF1078_RS16685 0.56 -3.0 50,161 + HMPREF1078_RS16685 0.57 -2.1 50,162 - HMPREF1078_RS16685 0.57 -0.3 50,165 - HMPREF1078_RS16685 0.58 -2.7 50,178 - HMPREF1078_RS16685 0.60 -3.8 50,253 - HMPREF1078_RS16685 0.74 -2.6 50,256 + HMPREF1078_RS16685 0.74 -1.2 50,257 - HMPREF1078_RS16685 0.75 -4.3 50,257 - HMPREF1078_RS16685 0.75 -3.5 50,257 - HMPREF1078_RS16685 0.75 -0.2 50,258 + HMPREF1078_RS16685 0.75 -0.3 50,346 + -1.3 50,348 - -2.8 50,351 + -4.0 50,376 - -3.2 50,376 - -4.5 50,376 - -3.0 50,396 + -1.6 50,396 + -2.8 50,414 + -1.6 50,415 - -0.6 50,424 + -3.2 50,424 + -2.8 50,425 - -0.4 50,436 + -2.9 50,466 + -3.4 50,467 - -3.9 50,467 - -3.3 50,467 - -3.8 50,471 + -4.1 50,471 + -2.7 50,472 - -1.8 50,627 + HMPREF1078_RS16690 0.13 -2.3 50,628 - HMPREF1078_RS16690 0.13 -5.1 50,628 - HMPREF1078_RS16690 0.13 -2.9 50,628 - HMPREF1078_RS16690 0.13 -3.3 50,687 + HMPREF1078_RS16690 0.16 -3.9 50,688 - HMPREF1078_RS16690 0.16 -0.8 50,793 - HMPREF1078_RS16690 0.23 -3.1
Or see this region's nucleotide sequence