Experiment: Beta-Lactose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS03470 and HMPREF1078_RS03475 overlap by 4 nucleotides HMPREF1078_RS03475 and HMPREF1078_RS03480 overlap by 10 nucleotides HMPREF1078_RS03480 and HMPREF1078_RS03485 are separated by 21 nucleotides
HMPREF1078_RS03470: HMPREF1078_RS03470 - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 820,033 to 820,872
_RS03470
HMPREF1078_RS03475: HMPREF1078_RS03475 - thiamine phosphate synthase, at 820,869 to 821,468
_RS03475
HMPREF1078_RS03480: HMPREF1078_RS03480 - thiamine phosphate synthase, at 821,459 to 822,196
_RS03480
HMPREF1078_RS03485: HMPREF1078_RS03485 - phosphomethylpyrimidine synthase ThiC, at 822,218 to 823,924
_RS03485
Position (kb)
821
822
823 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 820.503 kb on - strand, within HMPREF1078_RS03470 at 820.672 kb on - strand, within HMPREF1078_RS03470 at 820.715 kb on - strand, within HMPREF1078_RS03470 at 820.716 kb on + strand, within HMPREF1078_RS03470 at 820.748 kb on + strand, within HMPREF1078_RS03470 at 820.749 kb on - strand, within HMPREF1078_RS03470 at 820.749 kb on - strand, within HMPREF1078_RS03470 at 820.869 kb on - strand at 820.889 kb on + strand at 821.028 kb on + strand, within HMPREF1078_RS03475 at 821.071 kb on + strand, within HMPREF1078_RS03475 at 821.072 kb on - strand, within HMPREF1078_RS03475 at 821.077 kb on - strand, within HMPREF1078_RS03475 at 821.197 kb on - strand, within HMPREF1078_RS03475 at 821.227 kb on + strand, within HMPREF1078_RS03475 at 821.317 kb on + strand, within HMPREF1078_RS03475 at 821.317 kb on + strand, within HMPREF1078_RS03475 at 821.318 kb on - strand, within HMPREF1078_RS03475 at 821.327 kb on - strand, within HMPREF1078_RS03475 at 821.327 kb on - strand, within HMPREF1078_RS03475 at 821.350 kb on + strand, within HMPREF1078_RS03475 at 821.417 kb on + strand at 821.430 kb on - strand at 821.443 kb on - strand at 821.444 kb on + strand at 821.444 kb on + strand at 821.444 kb on + strand at 821.445 kb on - strand at 821.504 kb on + strand at 821.505 kb on - strand at 821.574 kb on - strand, within HMPREF1078_RS03480 at 821.605 kb on + strand, within HMPREF1078_RS03480 at 821.605 kb on + strand, within HMPREF1078_RS03480 at 821.800 kb on + strand, within HMPREF1078_RS03480 at 821.894 kb on + strand, within HMPREF1078_RS03480 at 821.894 kb on + strand, within HMPREF1078_RS03480 at 821.895 kb on - strand, within HMPREF1078_RS03480 at 821.895 kb on - strand, within HMPREF1078_RS03480 at 821.895 kb on - strand, within HMPREF1078_RS03480 at 821.898 kb on + strand, within HMPREF1078_RS03480 at 821.899 kb on - strand, within HMPREF1078_RS03480 at 821.919 kb on - strand, within HMPREF1078_RS03480 at 822.072 kb on + strand, within HMPREF1078_RS03480 at 822.161 kb on - strand at 822.194 kb on + strand at 822.195 kb on + strand at 822.217 kb on + strand at 822.218 kb on - strand at 822.218 kb on - strand at 822.237 kb on + strand at 822.237 kb on + strand at 822.248 kb on + strand at 822.269 kb on + strand at 822.285 kb on + strand at 822.285 kb on + strand at 822.285 kb on + strand at 822.286 kb on - strand at 822.286 kb on - strand at 822.389 kb on + strand, within HMPREF1078_RS03485 at 822.389 kb on + strand, within HMPREF1078_RS03485 at 822.389 kb on + strand, within HMPREF1078_RS03485 at 822.394 kb on + strand, within HMPREF1078_RS03485 at 822.407 kb on + strand, within HMPREF1078_RS03485 at 822.433 kb on + strand, within HMPREF1078_RS03485 at 822.433 kb on + strand, within HMPREF1078_RS03485 at 822.433 kb on + strand, within HMPREF1078_RS03485 at 822.579 kb on + strand, within HMPREF1078_RS03485 at 822.579 kb on + strand, within HMPREF1078_RS03485 at 822.579 kb on + strand, within HMPREF1078_RS03485 at 822.584 kb on + strand, within HMPREF1078_RS03485 at 822.621 kb on - strand, within HMPREF1078_RS03485 at 822.621 kb on - strand, within HMPREF1078_RS03485 at 822.658 kb on - strand, within HMPREF1078_RS03485 at 822.660 kb on - strand, within HMPREF1078_RS03485 at 822.710 kb on + strand, within HMPREF1078_RS03485 at 822.711 kb on - strand, within HMPREF1078_RS03485 at 822.839 kb on - strand, within HMPREF1078_RS03485 at 822.867 kb on + strand, within HMPREF1078_RS03485 at 822.891 kb on + strand, within HMPREF1078_RS03485 at 822.891 kb on + strand, within HMPREF1078_RS03485 at 822.946 kb on + strand, within HMPREF1078_RS03485 at 822.977 kb on - strand, within HMPREF1078_RS03485
Per-strain Table
Position Strand Gene LocusTag Fraction Beta-Lactose 10 mg/mL (C) remove 820,503 - HMPREF1078_RS03470 0.56 +0.6 820,672 - HMPREF1078_RS03470 0.76 -0.4 820,715 - HMPREF1078_RS03470 0.81 -0.1 820,716 + HMPREF1078_RS03470 0.81 +0.2 820,748 + HMPREF1078_RS03470 0.85 +0.7 820,749 - HMPREF1078_RS03470 0.85 +0.4 820,749 - HMPREF1078_RS03470 0.85 -0.4 820,869 - +0.0 820,889 + +0.7 821,028 + HMPREF1078_RS03475 0.27 +1.1 821,071 + HMPREF1078_RS03475 0.34 -0.4 821,072 - HMPREF1078_RS03475 0.34 -0.4 821,077 - HMPREF1078_RS03475 0.35 +1.5 821,197 - HMPREF1078_RS03475 0.55 +1.9 821,227 + HMPREF1078_RS03475 0.60 -1.1 821,317 + HMPREF1078_RS03475 0.75 -0.4 821,317 + HMPREF1078_RS03475 0.75 +0.4 821,318 - HMPREF1078_RS03475 0.75 -0.0 821,327 - HMPREF1078_RS03475 0.76 -0.7 821,327 - HMPREF1078_RS03475 0.76 -1.0 821,350 + HMPREF1078_RS03475 0.80 -0.6 821,417 + -1.2 821,430 - -0.5 821,443 - -0.3 821,444 + +0.9 821,444 + +0.0 821,444 + -0.4 821,445 - -0.8 821,504 + +0.1 821,505 - +1.2 821,574 - HMPREF1078_RS03480 0.16 -1.2 821,605 + HMPREF1078_RS03480 0.20 -2.4 821,605 + HMPREF1078_RS03480 0.20 +0.3 821,800 + HMPREF1078_RS03480 0.46 -1.1 821,894 + HMPREF1078_RS03480 0.59 -0.6 821,894 + HMPREF1078_RS03480 0.59 -2.1 821,895 - HMPREF1078_RS03480 0.59 -0.7 821,895 - HMPREF1078_RS03480 0.59 +2.4 821,895 - HMPREF1078_RS03480 0.59 -0.2 821,898 + HMPREF1078_RS03480 0.59 -0.5 821,899 - HMPREF1078_RS03480 0.60 -1.1 821,919 - HMPREF1078_RS03480 0.62 -0.2 822,072 + HMPREF1078_RS03480 0.83 +0.3 822,161 - -1.4 822,194 + +0.0 822,195 + -0.1 822,217 + -0.6 822,218 - +1.2 822,218 - -1.3 822,237 + -0.4 822,237 + -0.5 822,248 + -1.0 822,269 + -1.9 822,285 + -3.3 822,285 + -0.5 822,285 + -1.2 822,286 - -1.1 822,286 - +0.4 822,389 + HMPREF1078_RS03485 0.10 -0.9 822,389 + HMPREF1078_RS03485 0.10 -2.6 822,389 + HMPREF1078_RS03485 0.10 -0.1 822,394 + HMPREF1078_RS03485 0.10 -1.3 822,407 + HMPREF1078_RS03485 0.11 +0.6 822,433 + HMPREF1078_RS03485 0.13 -1.5 822,433 + HMPREF1078_RS03485 0.13 +0.3 822,433 + HMPREF1078_RS03485 0.13 -2.5 822,579 + HMPREF1078_RS03485 0.21 -0.1 822,579 + HMPREF1078_RS03485 0.21 +0.5 822,579 + HMPREF1078_RS03485 0.21 -0.1 822,584 + HMPREF1078_RS03485 0.21 -0.8 822,621 - HMPREF1078_RS03485 0.24 -0.4 822,621 - HMPREF1078_RS03485 0.24 -0.7 822,658 - HMPREF1078_RS03485 0.26 +0.9 822,660 - HMPREF1078_RS03485 0.26 +0.6 822,710 + HMPREF1078_RS03485 0.29 -1.5 822,711 - HMPREF1078_RS03485 0.29 +0.3 822,839 - HMPREF1078_RS03485 0.36 -0.4 822,867 + HMPREF1078_RS03485 0.38 +1.0 822,891 + HMPREF1078_RS03485 0.39 +0.1 822,891 + HMPREF1078_RS03485 0.39 -1.3 822,946 + HMPREF1078_RS03485 0.43 -0.7 822,977 - HMPREF1078_RS03485 0.44 -0.2
Or see this region's nucleotide sequence