Experiment: NAG 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS13375 and HMPREF1078_RS13380 are separated by 173 nucleotides HMPREF1078_RS13380 and HMPREF1078_RS13385 are separated by 13 nucleotides HMPREF1078_RS13385 and HMPREF1078_RS13390 are separated by 32 nucleotides HMPREF1078_RS13390 and HMPREF1078_RS13395 are separated by 4 nucleotides
HMPREF1078_RS13375: HMPREF1078_RS13375 - NADP-specific glutamate dehydrogenase, at 111,579 to 112,913
_RS13375
HMPREF1078_RS13380: HMPREF1078_RS13380 - hypothetical protein, at 113,087 to 113,269
_RS13380
HMPREF1078_RS13385: HMPREF1078_RS13385 - DUF2007 domain-containing protein, at 113,283 to 113,672
_RS13385
HMPREF1078_RS13390: HMPREF1078_RS13390 - protoporphyrinogen oxidase, at 113,705 to 115,081
_RS13390
HMPREF1078_RS13395: HMPREF1078_RS13395 - oxygen-independent coproporphyrinogen III oxidase, at 115,086 to 116,453
_RS13395
Position (kb)
113
114
115
116 Strain fitness (log2 ratio)
-2
-1
0
1 at 112.715 kb on + strand, within HMPREF1078_RS13375 at 112.749 kb on - strand, within HMPREF1078_RS13375 at 112.760 kb on + strand, within HMPREF1078_RS13375 at 112.810 kb on + strand at 112.884 kb on + strand at 112.926 kb on - strand at 112.939 kb on + strand at 112.939 kb on + strand at 113.115 kb on + strand, within HMPREF1078_RS13380 at 113.116 kb on - strand, within HMPREF1078_RS13380 at 113.116 kb on - strand, within HMPREF1078_RS13380 at 113.119 kb on + strand, within HMPREF1078_RS13380 at 113.188 kb on - strand, within HMPREF1078_RS13380 at 113.212 kb on + strand, within HMPREF1078_RS13380 at 113.234 kb on + strand, within HMPREF1078_RS13380 at 113.234 kb on + strand, within HMPREF1078_RS13380 at 113.235 kb on - strand, within HMPREF1078_RS13380 at 113.235 kb on - strand, within HMPREF1078_RS13380 at 113.235 kb on - strand, within HMPREF1078_RS13380 at 113.235 kb on - strand, within HMPREF1078_RS13380 at 113.269 kb on + strand at 113.311 kb on + strand at 113.338 kb on - strand, within HMPREF1078_RS13385 at 113.562 kb on - strand, within HMPREF1078_RS13385 at 113.562 kb on - strand, within HMPREF1078_RS13385 at 113.663 kb on + strand at 113.663 kb on + strand at 113.664 kb on - strand at 113.672 kb on + strand at 113.673 kb on - strand at 113.675 kb on + strand at 113.675 kb on + strand at 113.676 kb on - strand at 113.676 kb on - strand at 113.891 kb on - strand, within HMPREF1078_RS13390 at 114.003 kb on - strand, within HMPREF1078_RS13390 at 114.087 kb on - strand, within HMPREF1078_RS13390 at 114.173 kb on + strand, within HMPREF1078_RS13390 at 114.250 kb on + strand, within HMPREF1078_RS13390 at 114.250 kb on + strand, within HMPREF1078_RS13390 at 114.251 kb on - strand, within HMPREF1078_RS13390 at 114.251 kb on - strand, within HMPREF1078_RS13390 at 114.253 kb on - strand, within HMPREF1078_RS13390 at 114.621 kb on + strand, within HMPREF1078_RS13390 at 114.626 kb on + strand, within HMPREF1078_RS13390 at 114.626 kb on + strand, within HMPREF1078_RS13390 at 114.626 kb on + strand, within HMPREF1078_RS13390 at 114.626 kb on + strand, within HMPREF1078_RS13390 at 114.656 kb on + strand, within HMPREF1078_RS13390 at 114.897 kb on - strand, within HMPREF1078_RS13390 at 114.897 kb on - strand, within HMPREF1078_RS13390 at 114.897 kb on - strand, within HMPREF1078_RS13390 at 114.946 kb on + strand at 114.947 kb on - strand at 115.381 kb on + strand, within HMPREF1078_RS13395 at 115.382 kb on - strand, within HMPREF1078_RS13395 at 115.382 kb on - strand, within HMPREF1078_RS13395 at 115.382 kb on - strand, within HMPREF1078_RS13395 at 115.427 kb on - strand, within HMPREF1078_RS13395 at 115.575 kb on + strand, within HMPREF1078_RS13395
Per-strain Table
Position Strand Gene LocusTag Fraction NAG 10 mg/mL (C) remove 112,715 + HMPREF1078_RS13375 0.85 -0.6 112,749 - HMPREF1078_RS13375 0.88 -1.6 112,760 + HMPREF1078_RS13375 0.88 -2.1 112,810 + -1.0 112,884 + -1.5 112,926 - +1.2 112,939 + -1.5 112,939 + +0.1 113,115 + HMPREF1078_RS13380 0.15 +0.6 113,116 - HMPREF1078_RS13380 0.16 -0.8 113,116 - HMPREF1078_RS13380 0.16 +0.4 113,119 + HMPREF1078_RS13380 0.17 +0.2 113,188 - HMPREF1078_RS13380 0.55 +0.3 113,212 + HMPREF1078_RS13380 0.68 +1.4 113,234 + HMPREF1078_RS13380 0.80 +0.9 113,234 + HMPREF1078_RS13380 0.80 -1.7 113,235 - HMPREF1078_RS13380 0.81 -0.3 113,235 - HMPREF1078_RS13380 0.81 -0.9 113,235 - HMPREF1078_RS13380 0.81 +0.1 113,235 - HMPREF1078_RS13380 0.81 -0.4 113,269 + -0.6 113,311 + -0.2 113,338 - HMPREF1078_RS13385 0.14 -0.6 113,562 - HMPREF1078_RS13385 0.72 +0.3 113,562 - HMPREF1078_RS13385 0.72 +1.2 113,663 + +0.7 113,663 + -0.7 113,664 - -1.5 113,672 + +0.0 113,673 - +1.3 113,675 + +0.2 113,675 + +1.4 113,676 - +0.9 113,676 - +0.9 113,891 - HMPREF1078_RS13390 0.14 +0.3 114,003 - HMPREF1078_RS13390 0.22 -0.1 114,087 - HMPREF1078_RS13390 0.28 -0.5 114,173 + HMPREF1078_RS13390 0.34 -1.3 114,250 + HMPREF1078_RS13390 0.40 +0.5 114,250 + HMPREF1078_RS13390 0.40 -0.7 114,251 - HMPREF1078_RS13390 0.40 +1.0 114,251 - HMPREF1078_RS13390 0.40 +0.7 114,253 - HMPREF1078_RS13390 0.40 +0.6 114,621 + HMPREF1078_RS13390 0.67 +0.2 114,626 + HMPREF1078_RS13390 0.67 +0.5 114,626 + HMPREF1078_RS13390 0.67 -0.3 114,626 + HMPREF1078_RS13390 0.67 -0.4 114,626 + HMPREF1078_RS13390 0.67 -0.6 114,656 + HMPREF1078_RS13390 0.69 +1.3 114,897 - HMPREF1078_RS13390 0.87 +0.3 114,897 - HMPREF1078_RS13390 0.87 +0.0 114,897 - HMPREF1078_RS13390 0.87 +0.1 114,946 + +0.7 114,947 - +0.3 115,381 + HMPREF1078_RS13395 0.22 -0.8 115,382 - HMPREF1078_RS13395 0.22 -1.7 115,382 - HMPREF1078_RS13395 0.22 -0.2 115,382 - HMPREF1078_RS13395 0.22 +0.2 115,427 - HMPREF1078_RS13395 0.25 +1.1 115,575 + HMPREF1078_RS13395 0.36 -0.7
Or see this region's nucleotide sequence