Experiment: NAG 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS10430 and HMPREF1078_RS10435 are separated by 322 nucleotides HMPREF1078_RS10435 and HMPREF1078_RS10440 are separated by 100 nucleotides HMPREF1078_RS10440 and HMPREF1078_RS10445 are separated by 20 nucleotides HMPREF1078_RS10445 and HMPREF1078_RS10450 overlap by 4 nucleotides
HMPREF1078_RS10430: HMPREF1078_RS10430 - amidophosphoribosyltransferase, at 720,443 to 721,849
_RS10430
HMPREF1078_RS10435: HMPREF1078_RS10435 - tRNA-Thr, at 722,172 to 722,245
_RS10435
HMPREF1078_RS10440: HMPREF1078_RS10440 - DUF4199 domain-containing protein, at 722,346 to 722,876
_RS10440
HMPREF1078_RS10445: HMPREF1078_RS10445 - glycosyltransferase, at 722,897 to 723,850
_RS10445
HMPREF1078_RS10450: HMPREF1078_RS10450 - nitroreductase family protein, at 723,847 to 724,371
_RS10450
Position (kb)
722
723 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 721.355 kb on - strand, within HMPREF1078_RS10430 at 721.355 kb on - strand, within HMPREF1078_RS10430 at 721.355 kb on - strand, within HMPREF1078_RS10430 at 721.740 kb on + strand at 721.740 kb on + strand at 721.740 kb on + strand at 721.740 kb on + strand at 721.741 kb on - strand at 721.741 kb on - strand at 721.741 kb on - strand at 721.741 kb on - strand at 721.743 kb on + strand at 721.744 kb on + strand at 721.744 kb on + strand at 721.744 kb on + strand at 721.745 kb on - strand at 721.780 kb on + strand at 721.780 kb on + strand at 721.781 kb on - strand at 721.781 kb on - strand at 721.781 kb on - strand at 721.795 kb on - strand at 721.822 kb on + strand at 721.823 kb on - strand at 721.823 kb on - strand at 721.828 kb on + strand at 721.852 kb on + strand at 721.852 kb on + strand at 721.853 kb on - strand at 721.853 kb on - strand at 721.856 kb on + strand at 721.890 kb on + strand at 721.899 kb on - strand at 721.938 kb on + strand at 721.978 kb on - strand at 722.003 kb on - strand at 722.180 kb on - strand, within HMPREF1078_RS10435 at 722.279 kb on + strand at 722.288 kb on + strand at 722.288 kb on + strand at 722.288 kb on + strand at 722.289 kb on - strand at 722.290 kb on + strand at 722.291 kb on - strand at 722.307 kb on + strand at 722.342 kb on + strand at 722.365 kb on + strand at 722.388 kb on + strand at 722.399 kb on + strand, within HMPREF1078_RS10440 at 722.399 kb on + strand, within HMPREF1078_RS10440 at 722.407 kb on + strand, within HMPREF1078_RS10440 at 722.407 kb on + strand, within HMPREF1078_RS10440 at 722.407 kb on + strand, within HMPREF1078_RS10440 at 722.409 kb on + strand, within HMPREF1078_RS10440 at 722.409 kb on + strand, within HMPREF1078_RS10440 at 722.462 kb on + strand, within HMPREF1078_RS10440 at 722.472 kb on + strand, within HMPREF1078_RS10440 at 722.472 kb on + strand, within HMPREF1078_RS10440 at 722.482 kb on + strand, within HMPREF1078_RS10440 at 722.508 kb on + strand, within HMPREF1078_RS10440 at 722.539 kb on + strand, within HMPREF1078_RS10440 at 722.539 kb on + strand, within HMPREF1078_RS10440 at 722.539 kb on + strand, within HMPREF1078_RS10440 at 722.589 kb on + strand, within HMPREF1078_RS10440 at 722.589 kb on + strand, within HMPREF1078_RS10440 at 722.629 kb on + strand, within HMPREF1078_RS10440 at 722.686 kb on + strand, within HMPREF1078_RS10440 at 722.806 kb on + strand, within HMPREF1078_RS10440 at 722.806 kb on + strand, within HMPREF1078_RS10440 at 722.806 kb on + strand, within HMPREF1078_RS10440 at 723.846 kb on + strand at 723.847 kb on - strand at 723.857 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG 10 mg/mL (C) remove 721,355 - HMPREF1078_RS10430 0.65 +1.0 721,355 - HMPREF1078_RS10430 0.65 +1.8 721,355 - HMPREF1078_RS10430 0.65 +0.3 721,740 + +1.3 721,740 + +1.1 721,740 + -1.0 721,740 + -0.0 721,741 - -0.4 721,741 - -1.9 721,741 - +0.4 721,741 - -1.3 721,743 + -0.2 721,744 + -0.4 721,744 + +1.3 721,744 + -0.1 721,745 - -1.0 721,780 + -2.3 721,780 + -2.0 721,781 - +0.2 721,781 - -1.8 721,781 - +0.5 721,795 - +0.4 721,822 + +1.1 721,823 - -1.3 721,823 - +0.5 721,828 + +1.7 721,852 + +0.1 721,852 + +1.5 721,853 - +1.6 721,853 - +1.4 721,856 + -1.0 721,890 + -0.4 721,899 - -0.2 721,938 + -1.2 721,978 - +0.7 722,003 - +0.4 722,180 - HMPREF1078_RS10435 0.11 -1.9 722,279 + -2.5 722,288 + +0.9 722,288 + -0.6 722,288 + +0.0 722,289 - +2.4 722,290 + +1.2 722,291 - -0.9 722,307 + +0.3 722,342 + -0.3 722,365 + -0.2 722,388 + -1.2 722,399 + HMPREF1078_RS10440 0.10 +2.0 722,399 + HMPREF1078_RS10440 0.10 -0.6 722,407 + HMPREF1078_RS10440 0.11 -3.6 722,407 + HMPREF1078_RS10440 0.11 -1.0 722,407 + HMPREF1078_RS10440 0.11 -1.9 722,409 + HMPREF1078_RS10440 0.12 -0.5 722,409 + HMPREF1078_RS10440 0.12 -3.0 722,462 + HMPREF1078_RS10440 0.22 -2.5 722,472 + HMPREF1078_RS10440 0.24 -1.1 722,472 + HMPREF1078_RS10440 0.24 -2.6 722,482 + HMPREF1078_RS10440 0.26 -2.7 722,508 + HMPREF1078_RS10440 0.31 -1.4 722,539 + HMPREF1078_RS10440 0.36 -2.3 722,539 + HMPREF1078_RS10440 0.36 -2.1 722,539 + HMPREF1078_RS10440 0.36 -2.0 722,589 + HMPREF1078_RS10440 0.46 -1.4 722,589 + HMPREF1078_RS10440 0.46 +0.0 722,629 + HMPREF1078_RS10440 0.53 +0.8 722,686 + HMPREF1078_RS10440 0.64 -0.6 722,806 + HMPREF1078_RS10440 0.87 -1.0 722,806 + HMPREF1078_RS10440 0.87 +0.4 722,806 + HMPREF1078_RS10440 0.87 -2.7 723,846 + -3.9 723,847 - -1.7 723,857 + +1.4
Or see this region's nucleotide sequence