Experiment: D-Galactose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS00120 and HMPREF1078_RS00125 are separated by 22 nucleotides HMPREF1078_RS00125 and HMPREF1078_RS00130 overlap by 4 nucleotides HMPREF1078_RS00130 and HMPREF1078_RS00135 are separated by 10 nucleotides
HMPREF1078_RS00120: HMPREF1078_RS00120 - tryptophan synthase subunit alpha, at 28,390 to 29,160
_RS00120
HMPREF1078_RS00125: HMPREF1078_RS00125 - phosphoribosylanthranilate isomerase, at 29,183 to 29,848
_RS00125
HMPREF1078_RS00130: HMPREF1078_RS00130 - indole-3-glycerol phosphate synthase TrpC, at 29,845 to 30,654
_RS00130
HMPREF1078_RS00135: HMPREF1078_RS00135 - anthranilate phosphoribosyltransferase, at 30,665 to 31,669
_RS00135
Position (kb)
29
30 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 28.196 kb on + strand at 28.206 kb on + strand at 28.225 kb on - strand at 28.272 kb on + strand at 28.289 kb on - strand at 28.324 kb on - strand at 28.450 kb on + strand at 28.471 kb on + strand, within HMPREF1078_RS00120 at 28.472 kb on - strand, within HMPREF1078_RS00120 at 28.472 kb on - strand, within HMPREF1078_RS00120 at 28.472 kb on - strand, within HMPREF1078_RS00120 at 28.492 kb on - strand, within HMPREF1078_RS00120 at 28.494 kb on + strand, within HMPREF1078_RS00120 at 28.498 kb on + strand, within HMPREF1078_RS00120 at 28.499 kb on - strand, within HMPREF1078_RS00120 at 28.499 kb on - strand, within HMPREF1078_RS00120 at 28.640 kb on - strand, within HMPREF1078_RS00120 at 28.646 kb on + strand, within HMPREF1078_RS00120 at 28.647 kb on - strand, within HMPREF1078_RS00120 at 28.662 kb on + strand, within HMPREF1078_RS00120 at 28.662 kb on + strand, within HMPREF1078_RS00120 at 28.663 kb on - strand, within HMPREF1078_RS00120 at 28.663 kb on - strand, within HMPREF1078_RS00120 at 28.663 kb on - strand, within HMPREF1078_RS00120 at 28.716 kb on + strand, within HMPREF1078_RS00120 at 28.734 kb on - strand, within HMPREF1078_RS00120 at 28.754 kb on + strand, within HMPREF1078_RS00120 at 28.764 kb on + strand, within HMPREF1078_RS00120 at 28.766 kb on + strand, within HMPREF1078_RS00120 at 28.767 kb on - strand, within HMPREF1078_RS00120 at 28.767 kb on - strand, within HMPREF1078_RS00120 at 28.767 kb on - strand, within HMPREF1078_RS00120 at 28.767 kb on - strand, within HMPREF1078_RS00120 at 28.767 kb on - strand, within HMPREF1078_RS00120 at 28.804 kb on + strand, within HMPREF1078_RS00120 at 28.848 kb on - strand, within HMPREF1078_RS00120 at 28.909 kb on - strand, within HMPREF1078_RS00120 at 28.915 kb on + strand, within HMPREF1078_RS00120 at 29.027 kb on - strand, within HMPREF1078_RS00120 at 29.035 kb on + strand, within HMPREF1078_RS00120 at 29.035 kb on + strand, within HMPREF1078_RS00120 at 29.035 kb on - strand, within HMPREF1078_RS00120 at 29.036 kb on - strand, within HMPREF1078_RS00120 at 29.050 kb on + strand, within HMPREF1078_RS00120 at 29.059 kb on + strand, within HMPREF1078_RS00120 at 29.059 kb on + strand, within HMPREF1078_RS00120 at 29.223 kb on - strand at 29.231 kb on + strand at 29.232 kb on - strand at 29.309 kb on + strand, within HMPREF1078_RS00125 at 29.309 kb on + strand, within HMPREF1078_RS00125 at 29.310 kb on - strand, within HMPREF1078_RS00125 at 29.416 kb on - strand, within HMPREF1078_RS00125 at 29.419 kb on + strand, within HMPREF1078_RS00125 at 29.420 kb on - strand, within HMPREF1078_RS00125 at 29.662 kb on + strand, within HMPREF1078_RS00125 at 29.720 kb on + strand, within HMPREF1078_RS00125 at 29.720 kb on + strand, within HMPREF1078_RS00125 at 29.734 kb on + strand, within HMPREF1078_RS00125 at 29.738 kb on + strand, within HMPREF1078_RS00125 at 29.739 kb on - strand, within HMPREF1078_RS00125 at 29.766 kb on - strand, within HMPREF1078_RS00125 at 29.775 kb on - strand, within HMPREF1078_RS00125 at 29.851 kb on + strand at 29.852 kb on - strand at 29.861 kb on - strand at 29.887 kb on + strand at 29.887 kb on + strand at 29.990 kb on - strand, within HMPREF1078_RS00130 at 30.003 kb on + strand, within HMPREF1078_RS00130 at 30.067 kb on - strand, within HMPREF1078_RS00130 at 30.090 kb on + strand, within HMPREF1078_RS00130 at 30.106 kb on - strand, within HMPREF1078_RS00130 at 30.178 kb on - strand, within HMPREF1078_RS00130 at 30.198 kb on + strand, within HMPREF1078_RS00130 at 30.257 kb on - strand, within HMPREF1078_RS00130 at 30.350 kb on - strand, within HMPREF1078_RS00130 at 30.434 kb on - strand, within HMPREF1078_RS00130 at 30.435 kb on + strand, within HMPREF1078_RS00130 at 30.436 kb on - strand, within HMPREF1078_RS00130 at 30.451 kb on + strand, within HMPREF1078_RS00130 at 30.619 kb on + strand at 30.629 kb on - strand at 30.630 kb on + strand at 30.631 kb on - strand at 30.631 kb on - strand at 30.633 kb on + strand at 30.633 kb on + strand at 30.634 kb on - strand at 30.640 kb on + strand at 30.641 kb on - strand at 30.641 kb on - strand at 30.643 kb on + strand at 30.643 kb on + strand at 30.644 kb on - strand at 30.644 kb on - strand at 30.645 kb on + strand at 30.645 kb on + strand at 30.646 kb on - strand at 30.646 kb on - strand at 30.654 kb on + strand at 30.654 kb on + strand at 30.654 kb on + strand at 30.697 kb on + strand at 30.719 kb on - strand at 30.812 kb on + strand, within HMPREF1078_RS00135
Per-strain Table
Position Strand Gene LocusTag Fraction D-Galactose 10 mg/mL (C) remove 28,196 + +0.8 28,206 + +0.1 28,225 - +0.4 28,272 + +1.2 28,289 - +0.3 28,324 - -0.4 28,450 + -1.0 28,471 + HMPREF1078_RS00120 0.11 -3.3 28,472 - HMPREF1078_RS00120 0.11 -2.3 28,472 - HMPREF1078_RS00120 0.11 +0.3 28,472 - HMPREF1078_RS00120 0.11 -3.0 28,492 - HMPREF1078_RS00120 0.13 -2.3 28,494 + HMPREF1078_RS00120 0.13 -3.9 28,498 + HMPREF1078_RS00120 0.14 -2.1 28,499 - HMPREF1078_RS00120 0.14 -3.1 28,499 - HMPREF1078_RS00120 0.14 -2.5 28,640 - HMPREF1078_RS00120 0.32 -3.1 28,646 + HMPREF1078_RS00120 0.33 -0.1 28,647 - HMPREF1078_RS00120 0.33 -0.5 28,662 + HMPREF1078_RS00120 0.35 -4.6 28,662 + HMPREF1078_RS00120 0.35 -1.3 28,663 - HMPREF1078_RS00120 0.35 -3.0 28,663 - HMPREF1078_RS00120 0.35 -4.2 28,663 - HMPREF1078_RS00120 0.35 -2.5 28,716 + HMPREF1078_RS00120 0.42 -4.2 28,734 - HMPREF1078_RS00120 0.45 -3.3 28,754 + HMPREF1078_RS00120 0.47 -2.3 28,764 + HMPREF1078_RS00120 0.49 -3.7 28,766 + HMPREF1078_RS00120 0.49 -2.4 28,767 - HMPREF1078_RS00120 0.49 -4.2 28,767 - HMPREF1078_RS00120 0.49 -1.8 28,767 - HMPREF1078_RS00120 0.49 -1.1 28,767 - HMPREF1078_RS00120 0.49 -1.2 28,767 - HMPREF1078_RS00120 0.49 -4.1 28,804 + HMPREF1078_RS00120 0.54 -3.7 28,848 - HMPREF1078_RS00120 0.59 -6.3 28,909 - HMPREF1078_RS00120 0.67 -3.4 28,915 + HMPREF1078_RS00120 0.68 -4.4 29,027 - HMPREF1078_RS00120 0.83 -2.3 29,035 + HMPREF1078_RS00120 0.84 -3.9 29,035 + HMPREF1078_RS00120 0.84 -1.3 29,035 - HMPREF1078_RS00120 0.84 -3.8 29,036 - HMPREF1078_RS00120 0.84 -0.3 29,050 + HMPREF1078_RS00120 0.86 -3.3 29,059 + HMPREF1078_RS00120 0.87 -3.6 29,059 + HMPREF1078_RS00120 0.87 -2.9 29,223 - -0.4 29,231 + -3.7 29,232 - -2.9 29,309 + HMPREF1078_RS00125 0.19 -0.8 29,309 + HMPREF1078_RS00125 0.19 -1.8 29,310 - HMPREF1078_RS00125 0.19 -2.1 29,416 - HMPREF1078_RS00125 0.35 -2.3 29,419 + HMPREF1078_RS00125 0.35 -0.7 29,420 - HMPREF1078_RS00125 0.36 -3.6 29,662 + HMPREF1078_RS00125 0.72 -3.7 29,720 + HMPREF1078_RS00125 0.81 -2.9 29,720 + HMPREF1078_RS00125 0.81 -2.7 29,734 + HMPREF1078_RS00125 0.83 -0.5 29,738 + HMPREF1078_RS00125 0.83 -4.0 29,739 - HMPREF1078_RS00125 0.83 -5.9 29,766 - HMPREF1078_RS00125 0.88 -2.8 29,775 - HMPREF1078_RS00125 0.89 -3.0 29,851 + -2.1 29,852 - -0.7 29,861 - +0.1 29,887 + -3.4 29,887 + -2.7 29,990 - HMPREF1078_RS00130 0.18 -4.5 30,003 + HMPREF1078_RS00130 0.20 -3.1 30,067 - HMPREF1078_RS00130 0.27 -2.0 30,090 + HMPREF1078_RS00130 0.30 -4.0 30,106 - HMPREF1078_RS00130 0.32 -2.3 30,178 - HMPREF1078_RS00130 0.41 -3.5 30,198 + HMPREF1078_RS00130 0.44 -1.9 30,257 - HMPREF1078_RS00130 0.51 -3.9 30,350 - HMPREF1078_RS00130 0.62 -3.1 30,434 - HMPREF1078_RS00130 0.73 -1.1 30,435 + HMPREF1078_RS00130 0.73 -3.2 30,436 - HMPREF1078_RS00130 0.73 -1.3 30,451 + HMPREF1078_RS00130 0.75 -2.1 30,619 + -3.7 30,629 - -2.8 30,630 + -3.1 30,631 - -3.7 30,631 - -4.0 30,633 + -1.2 30,633 + -1.7 30,634 - -3.4 30,640 + -4.1 30,641 - -3.2 30,641 - -3.1 30,643 + -0.8 30,643 + -3.2 30,644 - -2.2 30,644 - -2.7 30,645 + -3.2 30,645 + -0.8 30,646 - -2.6 30,646 - -1.8 30,654 + -1.8 30,654 + -1.9 30,654 + -0.8 30,697 + -2.9 30,719 - -4.7 30,812 + HMPREF1078_RS00135 0.15 -4.0
Or see this region's nucleotide sequence