Experiment: Pectin from citrus peel; autoclaved 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS10165 and HMPREF1078_RS10170 are separated by 118 nucleotides HMPREF1078_RS10170 and HMPREF1078_RS10175 are separated by 254 nucleotides HMPREF1078_RS10175 and HMPREF1078_RS10180 are separated by 2 nucleotides HMPREF1078_RS10180 and HMPREF1078_RS10185 are separated by 79 nucleotides
HMPREF1078_RS10165: HMPREF1078_RS10165 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase, at 659,329 to 660,549
_RS10165
HMPREF1078_RS10170: HMPREF1078_RS10170 - N-acetyltransferase, at 660,668 to 661,198
_RS10170
HMPREF1078_RS10175: HMPREF1078_RS10175 - DUF2027 domain-containing protein, at 661,453 to 662,529
_RS10175
HMPREF1078_RS10180: HMPREF1078_RS10180 - CYTH domain-containing protein, at 662,532 to 662,999
_RS10180
HMPREF1078_RS10185: HMPREF1078_RS10185 - S46 family peptidase, at 663,079 to 665,238
_RS10185
Position (kb) 661
662
663 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 660.556 kb on + strand at 660.620 kb on + strand at 660.621 kb on - strand at 660.653 kb on + strand at 660.653 kb on + strand at 660.705 kb on - strand at 660.719 kb on - strand at 660.746 kb on - strand, within HMPREF1078_RS10170 at 660.777 kb on + strand, within HMPREF1078_RS10170 at 660.790 kb on - strand, within HMPREF1078_RS10170 at 660.918 kb on + strand, within HMPREF1078_RS10170 at 661.030 kb on - strand, within HMPREF1078_RS10170 at 661.036 kb on + strand, within HMPREF1078_RS10170 at 661.037 kb on - strand, within HMPREF1078_RS10170 at 661.037 kb on - strand, within HMPREF1078_RS10170 at 661.037 kb on - strand, within HMPREF1078_RS10170 at 661.037 kb on - strand, within HMPREF1078_RS10170 at 661.044 kb on - strand, within HMPREF1078_RS10170 at 661.075 kb on - strand, within HMPREF1078_RS10170 at 661.176 kb on - strand at 661.308 kb on + strand at 661.308 kb on + strand at 661.356 kb on - strand at 661.398 kb on + strand at 661.401 kb on + strand at 661.464 kb on - strand at 661.464 kb on - strand at 661.519 kb on - strand at 661.727 kb on + strand, within HMPREF1078_RS10175 at 661.811 kb on - strand, within HMPREF1078_RS10175 at 661.811 kb on - strand, within HMPREF1078_RS10175 at 661.815 kb on + strand, within HMPREF1078_RS10175 at 661.816 kb on - strand, within HMPREF1078_RS10175 at 661.831 kb on + strand, within HMPREF1078_RS10175 at 661.832 kb on - strand, within HMPREF1078_RS10175 at 661.851 kb on + strand, within HMPREF1078_RS10175 at 661.908 kb on + strand, within HMPREF1078_RS10175 at 661.909 kb on - strand, within HMPREF1078_RS10175 at 662.108 kb on + strand, within HMPREF1078_RS10175 at 662.121 kb on - strand, within HMPREF1078_RS10175 at 662.418 kb on - strand, within HMPREF1078_RS10175 at 662.464 kb on + strand at 662.465 kb on - strand at 662.516 kb on + strand at 662.603 kb on + strand, within HMPREF1078_RS10180 at 662.616 kb on + strand, within HMPREF1078_RS10180 at 662.618 kb on + strand, within HMPREF1078_RS10180 at 662.619 kb on - strand, within HMPREF1078_RS10180 at 662.737 kb on + strand, within HMPREF1078_RS10180 at 662.786 kb on + strand, within HMPREF1078_RS10180 at 662.798 kb on - strand, within HMPREF1078_RS10180 at 662.956 kb on - strand at 662.980 kb on - strand at 662.998 kb on - strand at 663.056 kb on + strand at 663.072 kb on + strand at 663.144 kb on + strand at 663.144 kb on + strand at 663.144 kb on + strand at 663.144 kb on + strand at 663.145 kb on - strand at 663.218 kb on - strand at 663.223 kb on - strand at 663.223 kb on - strand at 663.226 kb on + strand at 663.226 kb on + strand at 663.226 kb on + strand at 663.226 kb on + strand at 663.227 kb on - strand at 663.227 kb on - strand at 663.227 kb on - strand at 663.227 kb on - strand at 663.475 kb on + strand, within HMPREF1078_RS10185 at 663.476 kb on - strand, within HMPREF1078_RS10185 at 663.476 kb on - strand, within HMPREF1078_RS10185 at 663.514 kb on - strand, within HMPREF1078_RS10185
Per-strain Table
Position Strand Gene LocusTag Fraction Pectin from citrus peel; autoclaved 2.5 mg/mL (C) remove 660,556 + -3.1 660,620 + -1.3 660,621 - +1.4 660,653 + -0.4 660,653 + -0.5 660,705 - +0.0 660,719 - -3.6 660,746 - HMPREF1078_RS10170 0.15 -1.4 660,777 + HMPREF1078_RS10170 0.21 +0.0 660,790 - HMPREF1078_RS10170 0.23 +0.7 660,918 + HMPREF1078_RS10170 0.47 -0.0 661,030 - HMPREF1078_RS10170 0.68 -0.4 661,036 + HMPREF1078_RS10170 0.69 -3.3 661,037 - HMPREF1078_RS10170 0.69 -0.3 661,037 - HMPREF1078_RS10170 0.69 -0.8 661,037 - HMPREF1078_RS10170 0.69 -0.6 661,037 - HMPREF1078_RS10170 0.69 -1.1 661,044 - HMPREF1078_RS10170 0.71 -1.3 661,075 - HMPREF1078_RS10170 0.77 -1.2 661,176 - -0.5 661,308 + -0.5 661,308 + +0.6 661,356 - -0.3 661,398 + +0.8 661,401 + +0.5 661,464 - +0.4 661,464 - +0.9 661,519 - -0.8 661,727 + HMPREF1078_RS10175 0.25 -2.2 661,811 - HMPREF1078_RS10175 0.33 +2.5 661,811 - HMPREF1078_RS10175 0.33 +0.3 661,815 + HMPREF1078_RS10175 0.34 -1.8 661,816 - HMPREF1078_RS10175 0.34 +0.5 661,831 + HMPREF1078_RS10175 0.35 -0.7 661,832 - HMPREF1078_RS10175 0.35 -1.0 661,851 + HMPREF1078_RS10175 0.37 +2.3 661,908 + HMPREF1078_RS10175 0.42 -1.5 661,909 - HMPREF1078_RS10175 0.42 -2.0 662,108 + HMPREF1078_RS10175 0.61 -0.8 662,121 - HMPREF1078_RS10175 0.62 +0.8 662,418 - HMPREF1078_RS10175 0.90 -1.8 662,464 + -1.5 662,465 - -1.2 662,516 + +1.2 662,603 + HMPREF1078_RS10180 0.15 -0.8 662,616 + HMPREF1078_RS10180 0.18 +1.5 662,618 + HMPREF1078_RS10180 0.18 +1.0 662,619 - HMPREF1078_RS10180 0.19 +0.3 662,737 + HMPREF1078_RS10180 0.44 -0.2 662,786 + HMPREF1078_RS10180 0.54 -1.4 662,798 - HMPREF1078_RS10180 0.57 -0.7 662,956 - -0.1 662,980 - -1.1 662,998 - -2.4 663,056 + +0.5 663,072 + -0.2 663,144 + -0.5 663,144 + -2.0 663,144 + -0.8 663,144 + -1.6 663,145 - -0.2 663,218 - -2.5 663,223 - -0.3 663,223 - +0.8 663,226 + +0.9 663,226 + +0.7 663,226 + -0.5 663,226 + +0.2 663,227 - -3.1 663,227 - -1.8 663,227 - -1.1 663,227 - -0.3 663,475 + HMPREF1078_RS10185 0.18 +0.6 663,476 - HMPREF1078_RS10185 0.18 -1.5 663,476 - HMPREF1078_RS10185 0.18 -0.5 663,514 - HMPREF1078_RS10185 0.20 -1.4
Or see this region's nucleotide sequence