Experiment: Pectin from citrus peel; autoclaved 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS06960 and HMPREF1078_RS06965 are separated by 3 nucleotides HMPREF1078_RS06965 and HMPREF1078_RS06970 are separated by 118 nucleotides HMPREF1078_RS06970 and HMPREF1078_RS06975 are separated by 56 nucleotides HMPREF1078_RS06975 and HMPREF1078_RS06980 are separated by 70 nucleotides
HMPREF1078_RS06960: HMPREF1078_RS06960 - endonuclease/exonuclease/phosphatase family protein, at 800,613 to 801,491
_RS06960
HMPREF1078_RS06965: HMPREF1078_RS06965 - helix-hairpin-helix domain-containing protein, at 801,495 to 802,265
_RS06965
HMPREF1078_RS06970: HMPREF1078_RS06970 - sodium-dependent transporter, at 802,384 to 803,751
_RS06970
HMPREF1078_RS06975: HMPREF1078_RS06975 - ABC transporter ATP-binding protein, at 803,808 to 804,476
_RS06975
HMPREF1078_RS06980: HMPREF1078_RS06980 - tRNA threonylcarbamoyladenosine dehydratase, at 804,547 to 805,272
_RS06980
Position (kb) 802
803
804 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 801.414 kb on + strand at 801.414 kb on + strand at 801.431 kb on + strand at 801.475 kb on + strand at 801.496 kb on + strand at 801.497 kb on - strand at 801.511 kb on + strand at 801.512 kb on - strand at 801.638 kb on - strand, within HMPREF1078_RS06965 at 801.690 kb on + strand, within HMPREF1078_RS06965 at 801.691 kb on - strand, within HMPREF1078_RS06965 at 801.754 kb on + strand, within HMPREF1078_RS06965 at 801.806 kb on - strand, within HMPREF1078_RS06965 at 801.925 kb on - strand, within HMPREF1078_RS06965 at 801.925 kb on - strand, within HMPREF1078_RS06965 at 801.926 kb on - strand, within HMPREF1078_RS06965 at 801.930 kb on - strand, within HMPREF1078_RS06965 at 801.931 kb on + strand, within HMPREF1078_RS06965 at 801.932 kb on - strand, within HMPREF1078_RS06965 at 801.954 kb on - strand, within HMPREF1078_RS06965 at 801.968 kb on + strand, within HMPREF1078_RS06965 at 801.968 kb on + strand, within HMPREF1078_RS06965 at 801.989 kb on + strand, within HMPREF1078_RS06965 at 801.989 kb on + strand, within HMPREF1078_RS06965 at 802.176 kb on + strand, within HMPREF1078_RS06965 at 802.243 kb on + strand at 802.268 kb on + strand at 802.268 kb on + strand at 802.268 kb on + strand at 802.303 kb on + strand at 802.312 kb on + strand at 802.330 kb on - strand at 802.343 kb on - strand at 802.343 kb on - strand at 802.344 kb on + strand at 802.373 kb on - strand at 802.432 kb on + strand at 802.433 kb on - strand at 802.460 kb on + strand at 802.586 kb on - strand, within HMPREF1078_RS06970 at 802.660 kb on + strand, within HMPREF1078_RS06970 at 802.702 kb on + strand, within HMPREF1078_RS06970 at 802.761 kb on + strand, within HMPREF1078_RS06970 at 802.794 kb on + strand, within HMPREF1078_RS06970 at 802.824 kb on + strand, within HMPREF1078_RS06970 at 802.825 kb on - strand, within HMPREF1078_RS06970 at 802.825 kb on - strand, within HMPREF1078_RS06970 at 802.870 kb on + strand, within HMPREF1078_RS06970 at 802.871 kb on - strand, within HMPREF1078_RS06970 at 803.043 kb on - strand, within HMPREF1078_RS06970 at 803.148 kb on - strand, within HMPREF1078_RS06970 at 803.148 kb on - strand, within HMPREF1078_RS06970 at 803.188 kb on + strand, within HMPREF1078_RS06970 at 803.188 kb on + strand, within HMPREF1078_RS06970 at 803.189 kb on - strand, within HMPREF1078_RS06970 at 803.441 kb on + strand, within HMPREF1078_RS06970 at 803.481 kb on - strand, within HMPREF1078_RS06970 at 803.536 kb on - strand, within HMPREF1078_RS06970 at 803.536 kb on - strand, within HMPREF1078_RS06970 at 803.612 kb on + strand, within HMPREF1078_RS06970 at 803.616 kb on + strand at 803.617 kb on - strand at 803.652 kb on + strand at 803.652 kb on + strand at 803.653 kb on - strand at 803.653 kb on - strand at 803.654 kb on + strand at 803.704 kb on + strand at 803.789 kb on - strand at 804.535 kb on - strand at 804.535 kb on - strand at 804.574 kb on - strand at 804.574 kb on - strand at 804.579 kb on - strand at 804.587 kb on - strand at 804.587 kb on - strand at 804.591 kb on - strand at 804.613 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Pectin from citrus peel; autoclaved 2.5 mg/mL (C) remove 801,414 + +0.4 801,414 + -0.3 801,431 + -0.6 801,475 + -0.7 801,496 + -0.9 801,497 - +0.2 801,511 + +0.2 801,512 - +0.6 801,638 - HMPREF1078_RS06965 0.19 +0.1 801,690 + HMPREF1078_RS06965 0.25 +0.8 801,691 - HMPREF1078_RS06965 0.25 +0.0 801,754 + HMPREF1078_RS06965 0.34 +0.9 801,806 - HMPREF1078_RS06965 0.40 -2.4 801,925 - HMPREF1078_RS06965 0.56 -2.0 801,925 - HMPREF1078_RS06965 0.56 +1.2 801,926 - HMPREF1078_RS06965 0.56 -0.3 801,930 - HMPREF1078_RS06965 0.56 -1.3 801,931 + HMPREF1078_RS06965 0.57 +0.5 801,932 - HMPREF1078_RS06965 0.57 -0.8 801,954 - HMPREF1078_RS06965 0.60 +0.5 801,968 + HMPREF1078_RS06965 0.61 -1.2 801,968 + HMPREF1078_RS06965 0.61 -2.1 801,989 + HMPREF1078_RS06965 0.64 -2.2 801,989 + HMPREF1078_RS06965 0.64 +1.7 802,176 + HMPREF1078_RS06965 0.88 +0.6 802,243 + +0.5 802,268 + -0.0 802,268 + -0.2 802,268 + -1.9 802,303 + -2.5 802,312 + +0.5 802,330 - +0.3 802,343 - -0.2 802,343 - +0.4 802,344 + -2.4 802,373 - +0.2 802,432 + +0.9 802,433 - -1.6 802,460 + -2.1 802,586 - HMPREF1078_RS06970 0.15 -2.0 802,660 + HMPREF1078_RS06970 0.20 +1.3 802,702 + HMPREF1078_RS06970 0.23 -3.1 802,761 + HMPREF1078_RS06970 0.28 +1.3 802,794 + HMPREF1078_RS06970 0.30 -2.8 802,824 + HMPREF1078_RS06970 0.32 -3.1 802,825 - HMPREF1078_RS06970 0.32 -0.5 802,825 - HMPREF1078_RS06970 0.32 -2.4 802,870 + HMPREF1078_RS06970 0.36 -3.6 802,871 - HMPREF1078_RS06970 0.36 -0.7 803,043 - HMPREF1078_RS06970 0.48 +0.0 803,148 - HMPREF1078_RS06970 0.56 -1.1 803,148 - HMPREF1078_RS06970 0.56 -1.1 803,188 + HMPREF1078_RS06970 0.59 -3.9 803,188 + HMPREF1078_RS06970 0.59 -3.3 803,189 - HMPREF1078_RS06970 0.59 -4.0 803,441 + HMPREF1078_RS06970 0.77 -1.9 803,481 - HMPREF1078_RS06970 0.80 -1.9 803,536 - HMPREF1078_RS06970 0.84 -3.3 803,536 - HMPREF1078_RS06970 0.84 -0.6 803,612 + HMPREF1078_RS06970 0.90 -1.3 803,616 + -3.0 803,617 - -0.3 803,652 + -2.7 803,652 + -1.5 803,653 - -0.8 803,653 - -4.5 803,654 + -1.9 803,704 + -2.2 803,789 - +0.3 804,535 - +0.8 804,535 - +0.7 804,574 - -1.0 804,574 - -2.7 804,579 - +0.8 804,587 - +0.3 804,587 - +0.6 804,591 - +0.0 804,613 - -1.8
Or see this region's nucleotide sequence