Experiment: Pectin from citrus peel; autoclaved 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS04840 and HMPREF1078_RS04845 are separated by 14 nucleotides HMPREF1078_RS04845 and HMPREF1078_RS04850 are separated by 3 nucleotides
HMPREF1078_RS04840: HMPREF1078_RS04840 - type IX secretion system sortase PorU, at 217,137 to 220,502
_RS04840
HMPREF1078_RS04845: HMPREF1078_RS04845 - fumarylacetoacetate hydrolase family protein, at 220,517 to 221,131
_RS04845
HMPREF1078_RS04850: HMPREF1078_RS04850 - redox-sensing transcriptional repressor Rex, at 221,135 to 221,785
_RS04850
Position (kb)
221
222 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 220.157 kb on + strand, within HMPREF1078_RS04840 at 220.157 kb on + strand, within HMPREF1078_RS04840 at 220.172 kb on - strand at 220.228 kb on + strand at 220.233 kb on + strand at 220.255 kb on + strand at 220.255 kb on + strand at 220.255 kb on + strand at 220.255 kb on + strand at 220.256 kb on - strand at 220.259 kb on + strand at 220.259 kb on + strand at 220.300 kb on + strand at 220.300 kb on + strand at 220.301 kb on - strand at 220.301 kb on - strand at 220.301 kb on - strand at 220.363 kb on + strand at 220.368 kb on - strand at 220.368 kb on - strand at 220.429 kb on + strand at 220.429 kb on + strand at 220.437 kb on - strand at 220.488 kb on + strand at 220.488 kb on + strand at 220.488 kb on + strand at 220.489 kb on - strand at 220.489 kb on - strand at 220.518 kb on + strand at 220.518 kb on + strand at 220.555 kb on + strand at 220.555 kb on + strand at 220.555 kb on + strand at 220.558 kb on - strand at 220.558 kb on - strand at 220.629 kb on - strand, within HMPREF1078_RS04845 at 220.629 kb on - strand, within HMPREF1078_RS04845 at 220.656 kb on + strand, within HMPREF1078_RS04845 at 220.656 kb on + strand, within HMPREF1078_RS04845 at 220.687 kb on + strand, within HMPREF1078_RS04845 at 220.688 kb on - strand, within HMPREF1078_RS04845 at 220.697 kb on - strand, within HMPREF1078_RS04845 at 220.723 kb on + strand, within HMPREF1078_RS04845 at 220.946 kb on + strand, within HMPREF1078_RS04845 at 221.045 kb on - strand, within HMPREF1078_RS04845 at 221.054 kb on - strand, within HMPREF1078_RS04845 at 221.104 kb on - strand at 221.115 kb on - strand at 221.132 kb on - strand at 221.133 kb on + strand at 221.133 kb on + strand at 221.134 kb on - strand at 221.184 kb on + strand at 221.184 kb on + strand at 221.184 kb on + strand at 221.184 kb on + strand at 221.185 kb on - strand at 221.185 kb on - strand at 221.185 kb on - strand at 221.185 kb on - strand at 221.189 kb on - strand at 221.189 kb on - strand at 221.189 kb on - strand at 221.189 kb on - strand at 221.189 kb on - strand at 221.195 kb on - strand at 221.195 kb on - strand at 221.195 kb on - strand at 221.195 kb on - strand at 221.338 kb on - strand, within HMPREF1078_RS04850 at 221.515 kb on + strand, within HMPREF1078_RS04850 at 221.600 kb on - strand, within HMPREF1078_RS04850 at 221.666 kb on - strand, within HMPREF1078_RS04850 at 221.672 kb on + strand, within HMPREF1078_RS04850 at 221.718 kb on + strand, within HMPREF1078_RS04850 at 221.718 kb on + strand, within HMPREF1078_RS04850 at 221.800 kb on + strand at 221.800 kb on + strand at 221.801 kb on - strand at 221.806 kb on - strand at 221.907 kb on + strand at 221.909 kb on + strand at 221.910 kb on - strand at 221.936 kb on + strand at 221.937 kb on - strand at 221.937 kb on - strand at 221.939 kb on + strand at 221.987 kb on + strand at 221.991 kb on + strand at 221.992 kb on - strand at 222.007 kb on + strand at 222.016 kb on - strand at 222.048 kb on - strand at 222.053 kb on - strand at 222.164 kb on + strand at 222.164 kb on + strand at 222.165 kb on - strand at 222.185 kb on + strand at 222.186 kb on - strand at 222.376 kb on + strand at 222.382 kb on + strand at 222.406 kb on - strand at 222.469 kb on + strand at 222.472 kb on + strand at 222.488 kb on - strand at 222.505 kb on - strand at 222.521 kb on - strand at 222.528 kb on + strand at 222.528 kb on + strand at 222.606 kb on + strand at 222.731 kb on + strand at 222.732 kb on - strand at 222.732 kb on - strand at 222.734 kb on + strand at 222.735 kb on - strand at 222.738 kb on - strand at 222.738 kb on - strand at 222.741 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Pectin from citrus peel; autoclaved 2.5 mg/mL (C) remove 220,157 + HMPREF1078_RS04840 0.90 -2.0 220,157 + HMPREF1078_RS04840 0.90 -1.2 220,172 - -4.0 220,228 + -2.9 220,233 + -0.2 220,255 + +0.9 220,255 + -2.1 220,255 + +1.0 220,255 + -1.0 220,256 - -2.3 220,259 + -1.9 220,259 + -0.2 220,300 + -2.5 220,300 + -1.6 220,301 - -2.3 220,301 - -0.0 220,301 - -1.7 220,363 + -2.2 220,368 - -2.3 220,368 - -0.8 220,429 + -2.4 220,429 + -2.1 220,437 - -1.7 220,488 + -2.4 220,488 + -2.1 220,488 + -0.8 220,489 - -3.0 220,489 - -1.6 220,518 + +0.3 220,518 + -2.1 220,555 + +0.0 220,555 + +1.6 220,555 + -2.1 220,558 - +0.8 220,558 - +2.1 220,629 - HMPREF1078_RS04845 0.18 -0.1 220,629 - HMPREF1078_RS04845 0.18 +1.1 220,656 + HMPREF1078_RS04845 0.23 -0.5 220,656 + HMPREF1078_RS04845 0.23 -0.7 220,687 + HMPREF1078_RS04845 0.28 +1.3 220,688 - HMPREF1078_RS04845 0.28 -0.5 220,697 - HMPREF1078_RS04845 0.29 +1.0 220,723 + HMPREF1078_RS04845 0.33 -2.2 220,946 + HMPREF1078_RS04845 0.70 -1.4 221,045 - HMPREF1078_RS04845 0.86 +0.6 221,054 - HMPREF1078_RS04845 0.87 -0.8 221,104 - +0.4 221,115 - -3.5 221,132 - +0.2 221,133 + -1.8 221,133 + +0.9 221,134 - -1.1 221,184 + +0.3 221,184 + +1.7 221,184 + -2.1 221,184 + +0.0 221,185 - -1.0 221,185 - -1.8 221,185 - -1.2 221,185 - +1.8 221,189 - -0.2 221,189 - -0.7 221,189 - -0.4 221,189 - -1.7 221,189 - +0.4 221,195 - +1.0 221,195 - -0.1 221,195 - -0.2 221,195 - +0.2 221,338 - HMPREF1078_RS04850 0.31 -2.8 221,515 + HMPREF1078_RS04850 0.58 -2.6 221,600 - HMPREF1078_RS04850 0.71 +0.3 221,666 - HMPREF1078_RS04850 0.82 +0.4 221,672 + HMPREF1078_RS04850 0.82 -0.8 221,718 + HMPREF1078_RS04850 0.90 -2.2 221,718 + HMPREF1078_RS04850 0.90 -1.2 221,800 + -0.2 221,800 + -0.9 221,801 - -3.3 221,806 - -2.0 221,907 + -3.1 221,909 + -1.0 221,910 - -0.1 221,936 + -1.8 221,937 - -0.7 221,937 - +0.9 221,939 + -1.6 221,987 + +0.1 221,991 + -2.3 221,992 - -2.5 222,007 + +0.2 222,016 - -1.8 222,048 - -2.3 222,053 - -0.2 222,164 + -0.0 222,164 + +0.0 222,165 - -4.4 222,185 + +1.0 222,186 - -0.6 222,376 + +0.2 222,382 + -0.3 222,406 - +1.2 222,469 + +0.3 222,472 + +1.1 222,488 - +0.1 222,505 - +0.3 222,521 - -2.6 222,528 + +0.2 222,528 + +0.0 222,606 + +0.7 222,731 + -0.4 222,732 - +1.0 222,732 - -1.3 222,734 + -0.2 222,735 - -0.7 222,738 - +1.4 222,738 - +1.1 222,741 - +0.4
Or see this region's nucleotide sequence