Experiment: Pectin from citrus peel; autoclaved 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS19280 and HMPREF1078_RS02835 are separated by 91 nucleotides HMPREF1078_RS02835 and HMPREF1078_RS02840 are separated by 27 nucleotides HMPREF1078_RS02840 and HMPREF1078_RS19285 are separated by 311 nucleotides HMPREF1078_RS19285 and HMPREF1078_RS02850 overlap by 15 nucleotides HMPREF1078_RS02850 and HMPREF1078_RS19290 are separated by 536 nucleotides HMPREF1078_RS19290 and HMPREF1078_RS20050 overlap by 4 nucleotides HMPREF1078_RS20050 and HMPREF1078_RS02855 are separated by 12 nucleotides
HMPREF1078_RS19280: HMPREF1078_RS19280 - hypothetical protein, at 685,384 to 685,623
_RS19280
HMPREF1078_RS02835: HMPREF1078_RS02835 - hypothetical protein, at 685,715 to 686,011
_RS02835
HMPREF1078_RS02840: HMPREF1078_RS02840 - tRNA-Ile, at 686,039 to 686,113
_RS02840
HMPREF1078_RS19285: HMPREF1078_RS19285 - hypothetical protein, at 686,425 to 686,583
_RS19285
HMPREF1078_RS02850: HMPREF1078_RS02850 - hypothetical protein, at 686,569 to 686,757
_RS02850
HMPREF1078_RS19290: HMPREF1078_RS19290 - hypothetical protein, at 687,294 to 687,443
_RS19290
HMPREF1078_RS20050: HMPREF1078_RS20050 - hypothetical protein, at 687,440 to 687,565
_RS20050
HMPREF1078_RS02855: HMPREF1078_RS02855 - hypothetical protein, at 687,578 to 688,087
_RS02855
Position (kb)
686
687 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 685.592 kb on - strand, within HMPREF1078_RS19280 at 685.624 kb on - strand at 685.624 kb on - strand at 685.715 kb on - strand at 685.729 kb on - strand at 685.755 kb on - strand, within HMPREF1078_RS02835 at 685.829 kb on - strand, within HMPREF1078_RS02835 at 685.829 kb on - strand, within HMPREF1078_RS02835 at 685.873 kb on - strand, within HMPREF1078_RS02835 at 685.873 kb on - strand, within HMPREF1078_RS02835 at 685.912 kb on - strand, within HMPREF1078_RS02835 at 685.927 kb on - strand, within HMPREF1078_RS02835 at 686.121 kb on - strand at 686.221 kb on - strand at 686.293 kb on - strand at 686.354 kb on + strand at 686.355 kb on - strand at 686.355 kb on - strand at 686.355 kb on - strand at 686.356 kb on + strand at 686.357 kb on - strand at 686.376 kb on + strand at 686.411 kb on + strand at 686.411 kb on + strand at 686.425 kb on - strand at 686.466 kb on - strand, within HMPREF1078_RS19285 at 686.474 kb on + strand, within HMPREF1078_RS19285 at 686.475 kb on - strand, within HMPREF1078_RS19285 at 686.535 kb on + strand, within HMPREF1078_RS19285 at 686.535 kb on + strand, within HMPREF1078_RS19285 at 686.541 kb on - strand, within HMPREF1078_RS19285 at 686.612 kb on - strand, within HMPREF1078_RS02850 at 686.616 kb on - strand, within HMPREF1078_RS02850 at 686.616 kb on - strand, within HMPREF1078_RS02850 at 686.616 kb on - strand, within HMPREF1078_RS02850 at 686.628 kb on + strand, within HMPREF1078_RS02850 at 686.691 kb on - strand, within HMPREF1078_RS02850 at 686.738 kb on - strand, within HMPREF1078_RS02850 at 686.862 kb on - strand at 686.895 kb on - strand at 686.975 kb on - strand at 686.975 kb on - strand at 686.975 kb on - strand at 687.044 kb on - strand at 687.057 kb on - strand at 687.104 kb on - strand at 687.126 kb on - strand at 687.128 kb on - strand at 687.217 kb on - strand at 687.221 kb on - strand at 687.252 kb on - strand at 687.256 kb on - strand at 687.256 kb on - strand at 687.256 kb on - strand at 687.367 kb on - strand, within HMPREF1078_RS19290 at 687.367 kb on - strand, within HMPREF1078_RS19290 at 687.367 kb on - strand, within HMPREF1078_RS19290 at 687.367 kb on - strand, within HMPREF1078_RS19290 at 687.413 kb on - strand, within HMPREF1078_RS19290 at 687.413 kb on - strand, within HMPREF1078_RS19290 at 687.413 kb on - strand, within HMPREF1078_RS19290 at 687.415 kb on - strand, within HMPREF1078_RS19290 at 687.415 kb on - strand, within HMPREF1078_RS19290 at 687.415 kb on - strand, within HMPREF1078_RS19290 at 687.422 kb on - strand, within HMPREF1078_RS19290 at 687.426 kb on - strand, within HMPREF1078_RS19290 at 687.426 kb on - strand, within HMPREF1078_RS19290 at 687.426 kb on - strand, within HMPREF1078_RS19290 at 687.453 kb on - strand, within HMPREF1078_RS20050 at 687.526 kb on - strand, within HMPREF1078_RS20050 at 687.652 kb on - strand, within HMPREF1078_RS02855 at 687.652 kb on - strand, within HMPREF1078_RS02855
Per-strain Table
Position Strand Gene LocusTag Fraction Pectin from citrus peel; autoclaved 2.5 mg/mL (C) remove 685,592 - HMPREF1078_RS19280 0.87 -0.2 685,624 - -1.6 685,624 - -1.6 685,715 - +2.6 685,729 - -1.0 685,755 - HMPREF1078_RS02835 0.13 +0.4 685,829 - HMPREF1078_RS02835 0.38 +0.5 685,829 - HMPREF1078_RS02835 0.38 +1.3 685,873 - HMPREF1078_RS02835 0.53 -1.5 685,873 - HMPREF1078_RS02835 0.53 -0.2 685,912 - HMPREF1078_RS02835 0.66 -0.4 685,927 - HMPREF1078_RS02835 0.71 -0.7 686,121 - -1.2 686,221 - +0.2 686,293 - -0.8 686,354 + -0.7 686,355 - -3.2 686,355 - +1.1 686,355 - -0.2 686,356 + -1.4 686,357 - -0.3 686,376 + -0.6 686,411 + -0.9 686,411 + -3.1 686,425 - +0.4 686,466 - HMPREF1078_RS19285 0.26 -0.1 686,474 + HMPREF1078_RS19285 0.31 -0.9 686,475 - HMPREF1078_RS19285 0.31 +1.8 686,535 + HMPREF1078_RS19285 0.69 -2.3 686,535 + HMPREF1078_RS19285 0.69 -1.7 686,541 - HMPREF1078_RS19285 0.73 -0.5 686,612 - HMPREF1078_RS02850 0.23 -1.7 686,616 - HMPREF1078_RS02850 0.25 -0.8 686,616 - HMPREF1078_RS02850 0.25 -1.5 686,616 - HMPREF1078_RS02850 0.25 -0.2 686,628 + HMPREF1078_RS02850 0.31 -2.6 686,691 - HMPREF1078_RS02850 0.65 -0.8 686,738 - HMPREF1078_RS02850 0.89 -1.4 686,862 - +0.0 686,895 - -1.0 686,975 - -1.2 686,975 - -0.4 686,975 - +0.3 687,044 - +1.2 687,057 - -0.9 687,104 - +1.7 687,126 - -2.0 687,128 - +1.1 687,217 - -0.9 687,221 - -1.8 687,252 - +0.1 687,256 - -3.0 687,256 - -1.2 687,256 - +1.2 687,367 - HMPREF1078_RS19290 0.49 +0.6 687,367 - HMPREF1078_RS19290 0.49 +1.3 687,367 - HMPREF1078_RS19290 0.49 -1.0 687,367 - HMPREF1078_RS19290 0.49 +0.5 687,413 - HMPREF1078_RS19290 0.79 -1.2 687,413 - HMPREF1078_RS19290 0.79 +1.3 687,413 - HMPREF1078_RS19290 0.79 -0.1 687,415 - HMPREF1078_RS19290 0.81 -1.5 687,415 - HMPREF1078_RS19290 0.81 +0.0 687,415 - HMPREF1078_RS19290 0.81 -0.3 687,422 - HMPREF1078_RS19290 0.85 +1.2 687,426 - HMPREF1078_RS19290 0.88 +0.3 687,426 - HMPREF1078_RS19290 0.88 +0.7 687,426 - HMPREF1078_RS19290 0.88 -0.6 687,453 - HMPREF1078_RS20050 0.10 -0.1 687,526 - HMPREF1078_RS20050 0.68 +0.4 687,652 - HMPREF1078_RS02855 0.15 -0.3 687,652 - HMPREF1078_RS02855 0.15 -0.4
Or see this region's nucleotide sequence