Experiment: Pectin from citrus peel; autoclaved 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS00680 and HMPREF1078_RS00685 overlap by 10 nucleotides HMPREF1078_RS00685 and HMPREF1078_RS00690 are separated by 80 nucleotides HMPREF1078_RS00690 and HMPREF1078_RS00695 are separated by 36 nucleotides HMPREF1078_RS00695 and HMPREF1078_RS00700 are separated by 156 nucleotides HMPREF1078_RS00700 and HMPREF1078_RS00705 are separated by 22 nucleotides
HMPREF1078_RS00680: HMPREF1078_RS00680 - glycoside hydrolase family 97 protein, at 159,258 to 161,246
_RS00680
HMPREF1078_RS00685: HMPREF1078_RS00685 - MBL fold metallo-hydrolase, at 161,237 to 162,043
_RS00685
HMPREF1078_RS00690: HMPREF1078_RS00690 - tRNA-Cys, at 162,124 to 162,195
_RS00690
HMPREF1078_RS00695: HMPREF1078_RS00695 - tRNA-Cys, at 162,232 to 162,303
_RS00695
HMPREF1078_RS00700: HMPREF1078_RS00700 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 162,460 to 163,017
_RS00700
HMPREF1078_RS00705: HMPREF1078_RS00705 - dTDP-glucose 4,6-dehydratase, at 163,040 to 164,179
_RS00705
Position (kb) 161
162
163 Strain fitness (log2 ratio) -2
-1
0
1
2 at 160.251 kb on - strand, within HMPREF1078_RS00680 at 160.254 kb on + strand, within HMPREF1078_RS00680 at 160.254 kb on + strand, within HMPREF1078_RS00680 at 160.254 kb on + strand, within HMPREF1078_RS00680 at 160.255 kb on - strand, within HMPREF1078_RS00680 at 160.255 kb on - strand, within HMPREF1078_RS00680 at 160.255 kb on - strand, within HMPREF1078_RS00680 at 160.323 kb on - strand, within HMPREF1078_RS00680 at 160.381 kb on - strand, within HMPREF1078_RS00680 at 160.593 kb on - strand, within HMPREF1078_RS00680 at 160.623 kb on + strand, within HMPREF1078_RS00680 at 160.642 kb on - strand, within HMPREF1078_RS00680 at 160.685 kb on + strand, within HMPREF1078_RS00680 at 160.689 kb on + strand, within HMPREF1078_RS00680 at 160.689 kb on + strand, within HMPREF1078_RS00680 at 160.689 kb on + strand, within HMPREF1078_RS00680 at 160.689 kb on + strand, within HMPREF1078_RS00680 at 160.690 kb on - strand, within HMPREF1078_RS00680 at 160.690 kb on - strand, within HMPREF1078_RS00680 at 160.755 kb on - strand, within HMPREF1078_RS00680 at 160.803 kb on + strand, within HMPREF1078_RS00680 at 160.804 kb on - strand, within HMPREF1078_RS00680 at 160.805 kb on + strand, within HMPREF1078_RS00680 at 160.847 kb on + strand, within HMPREF1078_RS00680 at 160.847 kb on + strand, within HMPREF1078_RS00680 at 160.848 kb on - strand, within HMPREF1078_RS00680 at 160.851 kb on + strand, within HMPREF1078_RS00680 at 160.852 kb on - strand, within HMPREF1078_RS00680 at 160.852 kb on - strand, within HMPREF1078_RS00680 at 160.922 kb on + strand, within HMPREF1078_RS00680 at 160.952 kb on - strand, within HMPREF1078_RS00680 at 160.979 kb on + strand, within HMPREF1078_RS00680 at 161.171 kb on - strand at 161.171 kb on - strand at 161.244 kb on + strand at 161.244 kb on + strand at 161.245 kb on - strand at 161.251 kb on + strand at 161.308 kb on - strand at 161.315 kb on + strand at 161.315 kb on + strand at 161.324 kb on - strand, within HMPREF1078_RS00685 at 161.367 kb on + strand, within HMPREF1078_RS00685 at 161.367 kb on + strand, within HMPREF1078_RS00685 at 161.440 kb on + strand, within HMPREF1078_RS00685 at 161.441 kb on - strand, within HMPREF1078_RS00685 at 161.441 kb on - strand, within HMPREF1078_RS00685 at 161.441 kb on - strand, within HMPREF1078_RS00685 at 161.441 kb on - strand, within HMPREF1078_RS00685 at 161.517 kb on + strand, within HMPREF1078_RS00685 at 161.666 kb on - strand, within HMPREF1078_RS00685 at 161.782 kb on + strand, within HMPREF1078_RS00685 at 161.798 kb on - strand, within HMPREF1078_RS00685 at 161.830 kb on + strand, within HMPREF1078_RS00685 at 161.831 kb on - strand, within HMPREF1078_RS00685 at 161.831 kb on - strand, within HMPREF1078_RS00685 at 161.831 kb on - strand, within HMPREF1078_RS00685 at 161.840 kb on - strand, within HMPREF1078_RS00685 at 161.840 kb on - strand, within HMPREF1078_RS00685 at 161.912 kb on + strand, within HMPREF1078_RS00685 at 161.912 kb on + strand, within HMPREF1078_RS00685 at 161.913 kb on - strand, within HMPREF1078_RS00685 at 161.913 kb on - strand, within HMPREF1078_RS00685 at 161.945 kb on - strand, within HMPREF1078_RS00685 at 161.990 kb on + strand at 161.991 kb on - strand at 162.004 kb on - strand at 162.050 kb on - strand at 162.108 kb on + strand at 162.109 kb on - strand at 162.109 kb on - strand at 162.150 kb on + strand, within HMPREF1078_RS00690 at 162.150 kb on + strand, within HMPREF1078_RS00690 at 162.174 kb on + strand, within HMPREF1078_RS00690 at 162.175 kb on - strand, within HMPREF1078_RS00690 at 162.201 kb on - strand at 162.201 kb on - strand at 162.226 kb on + strand at 162.226 kb on + strand at 162.227 kb on - strand at 162.426 kb on + strand at 162.438 kb on + strand at 163.020 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Pectin from citrus peel; autoclaved 2.5 mg/mL (C) remove 160,251 - HMPREF1078_RS00680 0.50 +1.1 160,254 + HMPREF1078_RS00680 0.50 -0.9 160,254 + HMPREF1078_RS00680 0.50 -0.4 160,254 + HMPREF1078_RS00680 0.50 +1.7 160,255 - HMPREF1078_RS00680 0.50 -1.1 160,255 - HMPREF1078_RS00680 0.50 -0.5 160,255 - HMPREF1078_RS00680 0.50 +0.2 160,323 - HMPREF1078_RS00680 0.54 -2.5 160,381 - HMPREF1078_RS00680 0.56 +0.2 160,593 - HMPREF1078_RS00680 0.67 +0.2 160,623 + HMPREF1078_RS00680 0.69 -0.5 160,642 - HMPREF1078_RS00680 0.70 -2.0 160,685 + HMPREF1078_RS00680 0.72 +0.2 160,689 + HMPREF1078_RS00680 0.72 +0.9 160,689 + HMPREF1078_RS00680 0.72 -2.5 160,689 + HMPREF1078_RS00680 0.72 +0.5 160,689 + HMPREF1078_RS00680 0.72 -0.4 160,690 - HMPREF1078_RS00680 0.72 +1.3 160,690 - HMPREF1078_RS00680 0.72 +0.1 160,755 - HMPREF1078_RS00680 0.75 -1.7 160,803 + HMPREF1078_RS00680 0.78 +1.9 160,804 - HMPREF1078_RS00680 0.78 +0.1 160,805 + HMPREF1078_RS00680 0.78 +0.4 160,847 + HMPREF1078_RS00680 0.80 -0.7 160,847 + HMPREF1078_RS00680 0.80 -1.0 160,848 - HMPREF1078_RS00680 0.80 -0.6 160,851 + HMPREF1078_RS00680 0.80 +0.3 160,852 - HMPREF1078_RS00680 0.80 +0.6 160,852 - HMPREF1078_RS00680 0.80 -2.5 160,922 + HMPREF1078_RS00680 0.84 -0.5 160,952 - HMPREF1078_RS00680 0.85 +0.3 160,979 + HMPREF1078_RS00680 0.87 -0.4 161,171 - -0.2 161,171 - -0.8 161,244 + +0.8 161,244 + -0.3 161,245 - +0.1 161,251 + +0.8 161,308 - -1.8 161,315 + -0.7 161,315 + -1.3 161,324 - HMPREF1078_RS00685 0.11 +0.3 161,367 + HMPREF1078_RS00685 0.16 +1.1 161,367 + HMPREF1078_RS00685 0.16 +1.5 161,440 + HMPREF1078_RS00685 0.25 -0.8 161,441 - HMPREF1078_RS00685 0.25 -0.8 161,441 - HMPREF1078_RS00685 0.25 +1.6 161,441 - HMPREF1078_RS00685 0.25 -0.3 161,441 - HMPREF1078_RS00685 0.25 -0.6 161,517 + HMPREF1078_RS00685 0.35 -0.4 161,666 - HMPREF1078_RS00685 0.53 +0.0 161,782 + HMPREF1078_RS00685 0.68 -1.1 161,798 - HMPREF1078_RS00685 0.70 +0.1 161,830 + HMPREF1078_RS00685 0.73 -0.2 161,831 - HMPREF1078_RS00685 0.74 +1.9 161,831 - HMPREF1078_RS00685 0.74 +0.5 161,831 - HMPREF1078_RS00685 0.74 -0.2 161,840 - HMPREF1078_RS00685 0.75 -0.1 161,840 - HMPREF1078_RS00685 0.75 +1.8 161,912 + HMPREF1078_RS00685 0.84 +0.8 161,912 + HMPREF1078_RS00685 0.84 -0.3 161,913 - HMPREF1078_RS00685 0.84 +0.1 161,913 - HMPREF1078_RS00685 0.84 -1.7 161,945 - HMPREF1078_RS00685 0.88 -0.5 161,990 + -1.6 161,991 - +2.0 162,004 - -1.1 162,050 - -1.8 162,108 + +1.6 162,109 - -2.7 162,109 - +0.2 162,150 + HMPREF1078_RS00690 0.36 -1.6 162,150 + HMPREF1078_RS00690 0.36 +0.8 162,174 + HMPREF1078_RS00690 0.69 +1.3 162,175 - HMPREF1078_RS00690 0.71 -0.1 162,201 - +0.3 162,201 - +0.1 162,226 + -0.5 162,226 + -0.5 162,227 - -0.5 162,426 + -2.3 162,438 + -1.8 163,020 + +0.7
Or see this region's nucleotide sequence