Experiment: M9 with Supernatant; Pseudomonas DC3000 grown in LB 0.25X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt JOY50_RS01170 and JOY50_RS01175 are separated by 171 nucleotides JOY50_RS01175 and JOY50_RS01180 are separated by 63 nucleotides JOY50_RS01180 and JOY50_RS01185 are separated by 135 nucleotides
JOY50_RS01170: JOY50_RS01170 - TolC family outer membrane protein, at 258,977 to 260,335
_RS01170
JOY50_RS01175: JOY50_RS01175 - tRNA-uridine aminocarboxypropyltransferase, at 260,507 to 261,106
_RS01175
JOY50_RS01180: JOY50_RS01180 - PAS domain-containing methyl-accepting chemotaxis protein, at 261,170 to 262,735
_RS01180
JOY50_RS01185: JOY50_RS01185 - LysR family transcriptional regulator, at 262,871 to 263,764
_RS01185
Position (kb) 261
262
263 Strain fitness (log2 ratio) -2
-1
0
1
2 at 260.172 kb on - strand, within JOY50_RS01170 at 260.194 kb on + strand, within JOY50_RS01170 at 260.202 kb on - strand at 260.209 kb on + strand at 260.209 kb on + strand at 260.246 kb on + strand at 260.274 kb on + strand at 260.292 kb on + strand at 260.307 kb on + strand at 260.451 kb on - strand at 260.505 kb on + strand at 260.513 kb on - strand at 260.513 kb on - strand at 260.536 kb on + strand at 260.544 kb on - strand at 260.550 kb on + strand at 260.550 kb on + strand at 260.550 kb on + strand at 260.550 kb on + strand at 260.558 kb on - strand at 260.558 kb on - strand at 260.558 kb on - strand at 260.558 kb on - strand at 260.558 kb on - strand at 260.563 kb on - strand at 260.699 kb on + strand, within JOY50_RS01175 at 260.759 kb on - strand, within JOY50_RS01175 at 260.832 kb on - strand, within JOY50_RS01175 at 260.864 kb on + strand, within JOY50_RS01175 at 260.864 kb on + strand, within JOY50_RS01175 at 260.950 kb on + strand, within JOY50_RS01175 at 260.958 kb on - strand, within JOY50_RS01175 at 260.973 kb on + strand, within JOY50_RS01175 at 260.975 kb on + strand, within JOY50_RS01175 at 260.981 kb on - strand, within JOY50_RS01175 at 260.981 kb on - strand, within JOY50_RS01175 at 260.983 kb on - strand, within JOY50_RS01175 at 260.988 kb on - strand, within JOY50_RS01175 at 261.064 kb on + strand at 261.066 kb on - strand at 261.096 kb on + strand at 261.187 kb on + strand at 261.187 kb on + strand at 261.187 kb on + strand at 261.187 kb on + strand at 261.195 kb on - strand at 261.195 kb on - strand at 261.195 kb on - strand at 261.195 kb on - strand at 261.195 kb on - strand at 261.211 kb on - strand at 261.224 kb on + strand at 261.256 kb on + strand at 261.350 kb on - strand, within JOY50_RS01180 at 261.393 kb on - strand, within JOY50_RS01180 at 261.403 kb on - strand, within JOY50_RS01180 at 261.423 kb on - strand, within JOY50_RS01180 at 261.423 kb on - strand, within JOY50_RS01180 at 261.461 kb on - strand, within JOY50_RS01180 at 261.507 kb on - strand, within JOY50_RS01180 at 261.611 kb on + strand, within JOY50_RS01180 at 261.617 kb on - strand, within JOY50_RS01180 at 261.706 kb on + strand, within JOY50_RS01180 at 261.760 kb on + strand, within JOY50_RS01180 at 261.760 kb on - strand, within JOY50_RS01180 at 261.760 kb on - strand, within JOY50_RS01180 at 261.760 kb on - strand, within JOY50_RS01180 at 261.770 kb on + strand, within JOY50_RS01180 at 261.775 kb on - strand, within JOY50_RS01180 at 261.775 kb on - strand, within JOY50_RS01180 at 261.805 kb on + strand, within JOY50_RS01180 at 261.821 kb on + strand, within JOY50_RS01180 at 261.854 kb on - strand, within JOY50_RS01180 at 261.911 kb on - strand, within JOY50_RS01180 at 262.004 kb on + strand, within JOY50_RS01180 at 262.004 kb on + strand, within JOY50_RS01180 at 262.004 kb on + strand, within JOY50_RS01180 at 262.012 kb on - strand, within JOY50_RS01180 at 262.012 kb on - strand, within JOY50_RS01180 at 262.012 kb on - strand, within JOY50_RS01180 at 262.012 kb on - strand, within JOY50_RS01180 at 262.040 kb on - strand, within JOY50_RS01180 at 262.048 kb on + strand, within JOY50_RS01180 at 262.048 kb on + strand, within JOY50_RS01180 at 262.056 kb on - strand, within JOY50_RS01180 at 262.056 kb on - strand, within JOY50_RS01180 at 262.106 kb on + strand, within JOY50_RS01180 at 262.106 kb on + strand, within JOY50_RS01180 at 262.106 kb on + strand, within JOY50_RS01180 at 262.106 kb on + strand, within JOY50_RS01180 at 262.107 kb on + strand, within JOY50_RS01180 at 262.113 kb on + strand, within JOY50_RS01180 at 262.114 kb on - strand, within JOY50_RS01180 at 262.114 kb on - strand, within JOY50_RS01180 at 262.121 kb on - strand, within JOY50_RS01180 at 262.168 kb on - strand, within JOY50_RS01180 at 262.175 kb on - strand, within JOY50_RS01180 at 262.250 kb on + strand, within JOY50_RS01180 at 262.253 kb on - strand, within JOY50_RS01180 at 262.255 kb on + strand, within JOY50_RS01180 at 262.255 kb on + strand, within JOY50_RS01180 at 262.258 kb on - strand, within JOY50_RS01180 at 262.263 kb on - strand, within JOY50_RS01180 at 262.273 kb on - strand, within JOY50_RS01180 at 262.277 kb on + strand, within JOY50_RS01180 at 262.285 kb on - strand, within JOY50_RS01180 at 262.289 kb on + strand, within JOY50_RS01180 at 262.289 kb on + strand, within JOY50_RS01180 at 262.289 kb on + strand, within JOY50_RS01180 at 262.291 kb on - strand, within JOY50_RS01180 at 262.308 kb on + strand, within JOY50_RS01180 at 262.321 kb on - strand, within JOY50_RS01180 at 262.321 kb on - strand, within JOY50_RS01180 at 262.321 kb on - strand, within JOY50_RS01180 at 262.379 kb on + strand, within JOY50_RS01180 at 262.379 kb on + strand, within JOY50_RS01180 at 262.406 kb on - strand, within JOY50_RS01180 at 262.408 kb on - strand, within JOY50_RS01180 at 262.408 kb on - strand, within JOY50_RS01180 at 262.513 kb on + strand, within JOY50_RS01180 at 262.898 kb on - strand at 262.919 kb on - strand at 263.024 kb on - strand, within JOY50_RS01185 at 263.024 kb on - strand, within JOY50_RS01185 at 263.029 kb on - strand, within JOY50_RS01185 at 263.029 kb on - strand, within JOY50_RS01185 at 263.077 kb on - strand, within JOY50_RS01185 at 263.104 kb on - strand, within JOY50_RS01185 at 263.124 kb on - strand, within JOY50_RS01185 at 263.135 kb on - strand, within JOY50_RS01185 at 263.174 kb on - strand, within JOY50_RS01185 at 263.191 kb on + strand, within JOY50_RS01185 at 263.204 kb on - strand, within JOY50_RS01185 at 263.204 kb on - strand, within JOY50_RS01185 at 263.206 kb on + strand, within JOY50_RS01185 at 263.219 kb on - strand, within JOY50_RS01185 at 263.219 kb on - strand, within JOY50_RS01185 at 263.219 kb on - strand, within JOY50_RS01185 at 263.219 kb on - strand, within JOY50_RS01185 at 263.254 kb on - strand, within JOY50_RS01185 at 263.256 kb on - strand, within JOY50_RS01185 at 263.276 kb on - strand, within JOY50_RS01185 at 263.288 kb on - strand, within JOY50_RS01185 at 263.288 kb on - strand, within JOY50_RS01185 at 263.335 kb on - strand, within JOY50_RS01185 at 263.345 kb on - strand, within JOY50_RS01185 at 263.365 kb on - strand, within JOY50_RS01185 at 263.414 kb on - strand, within JOY50_RS01185 at 263.453 kb on - strand, within JOY50_RS01185 at 263.469 kb on - strand, within JOY50_RS01185 at 263.469 kb on - strand, within JOY50_RS01185 at 263.491 kb on - strand, within JOY50_RS01185 at 263.498 kb on - strand, within JOY50_RS01185 at 263.498 kb on - strand, within JOY50_RS01185 at 263.500 kb on - strand, within JOY50_RS01185 at 263.500 kb on - strand, within JOY50_RS01185 at 263.520 kb on - strand, within JOY50_RS01185 at 263.525 kb on - strand, within JOY50_RS01185 at 263.552 kb on - strand, within JOY50_RS01185 at 263.589 kb on - strand, within JOY50_RS01185 at 263.590 kb on - strand, within JOY50_RS01185 at 263.652 kb on - strand, within JOY50_RS01185 at 263.652 kb on - strand, within JOY50_RS01185
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant; Pseudomonas DC3000 grown in LB 0.25X remove 260,172 - JOY50_RS01170 0.88 +0.3 260,194 + JOY50_RS01170 0.90 -1.5 260,202 - +0.3 260,209 + +0.4 260,209 + +0.9 260,246 + +0.2 260,274 + +1.4 260,292 + +0.9 260,307 + +0.8 260,451 - +0.0 260,505 + +0.8 260,513 - -0.4 260,513 - -0.4 260,536 + -0.1 260,544 - +0.1 260,550 + -0.3 260,550 + -0.8 260,550 + -0.5 260,550 + -0.1 260,558 - +1.2 260,558 - +0.2 260,558 - -2.1 260,558 - +0.2 260,558 - -0.3 260,563 - -1.1 260,699 + JOY50_RS01175 0.32 -0.2 260,759 - JOY50_RS01175 0.42 +0.9 260,832 - JOY50_RS01175 0.54 -0.2 260,864 + JOY50_RS01175 0.59 -1.5 260,864 + JOY50_RS01175 0.59 +0.7 260,950 + JOY50_RS01175 0.74 +0.2 260,958 - JOY50_RS01175 0.75 +0.1 260,973 + JOY50_RS01175 0.78 -1.6 260,975 + JOY50_RS01175 0.78 -0.5 260,981 - JOY50_RS01175 0.79 -1.0 260,981 - JOY50_RS01175 0.79 +0.8 260,983 - JOY50_RS01175 0.79 -1.1 260,988 - JOY50_RS01175 0.80 -0.5 261,064 + -1.1 261,066 - -0.1 261,096 + +0.1 261,187 + -0.1 261,187 + +1.1 261,187 + -0.2 261,187 + -0.1 261,195 - +0.5 261,195 - +0.2 261,195 - -0.2 261,195 - -0.4 261,195 - -0.6 261,211 - +0.1 261,224 + -1.1 261,256 + -0.8 261,350 - JOY50_RS01180 0.11 +0.4 261,393 - JOY50_RS01180 0.14 -0.6 261,403 - JOY50_RS01180 0.15 -1.3 261,423 - JOY50_RS01180 0.16 -1.3 261,423 - JOY50_RS01180 0.16 -2.2 261,461 - JOY50_RS01180 0.19 +0.7 261,507 - JOY50_RS01180 0.22 -0.3 261,611 + JOY50_RS01180 0.28 +0.7 261,617 - JOY50_RS01180 0.29 -0.9 261,706 + JOY50_RS01180 0.34 +0.8 261,760 + JOY50_RS01180 0.38 -0.3 261,760 - JOY50_RS01180 0.38 -0.4 261,760 - JOY50_RS01180 0.38 +0.0 261,760 - JOY50_RS01180 0.38 +0.9 261,770 + JOY50_RS01180 0.38 +1.2 261,775 - JOY50_RS01180 0.39 +0.3 261,775 - JOY50_RS01180 0.39 +0.0 261,805 + JOY50_RS01180 0.41 -1.6 261,821 + JOY50_RS01180 0.42 +0.6 261,854 - JOY50_RS01180 0.44 -0.2 261,911 - JOY50_RS01180 0.47 -0.6 262,004 + JOY50_RS01180 0.53 -0.5 262,004 + JOY50_RS01180 0.53 +0.3 262,004 + JOY50_RS01180 0.53 +0.5 262,012 - JOY50_RS01180 0.54 -0.4 262,012 - JOY50_RS01180 0.54 -0.7 262,012 - JOY50_RS01180 0.54 +0.5 262,012 - JOY50_RS01180 0.54 -1.1 262,040 - JOY50_RS01180 0.56 -0.6 262,048 + JOY50_RS01180 0.56 +1.2 262,048 + JOY50_RS01180 0.56 +0.4 262,056 - JOY50_RS01180 0.57 +0.2 262,056 - JOY50_RS01180 0.57 +0.8 262,106 + JOY50_RS01180 0.60 +0.2 262,106 + JOY50_RS01180 0.60 -0.2 262,106 + JOY50_RS01180 0.60 +0.6 262,106 + JOY50_RS01180 0.60 -0.3 262,107 + JOY50_RS01180 0.60 +0.5 262,113 + JOY50_RS01180 0.60 -1.6 262,114 - JOY50_RS01180 0.60 +0.2 262,114 - JOY50_RS01180 0.60 +2.3 262,121 - JOY50_RS01180 0.61 -0.1 262,168 - JOY50_RS01180 0.64 +0.1 262,175 - JOY50_RS01180 0.64 +1.4 262,250 + JOY50_RS01180 0.69 -0.8 262,253 - JOY50_RS01180 0.69 +1.1 262,255 + JOY50_RS01180 0.69 -0.3 262,255 + JOY50_RS01180 0.69 -0.3 262,258 - JOY50_RS01180 0.69 -0.0 262,263 - JOY50_RS01180 0.70 +1.3 262,273 - JOY50_RS01180 0.70 +0.9 262,277 + JOY50_RS01180 0.71 -0.3 262,285 - JOY50_RS01180 0.71 +0.5 262,289 + JOY50_RS01180 0.71 +0.8 262,289 + JOY50_RS01180 0.71 +0.7 262,289 + JOY50_RS01180 0.71 +0.3 262,291 - JOY50_RS01180 0.72 +1.1 262,308 + JOY50_RS01180 0.73 -0.9 262,321 - JOY50_RS01180 0.73 -0.4 262,321 - JOY50_RS01180 0.73 -0.1 262,321 - JOY50_RS01180 0.73 +0.9 262,379 + JOY50_RS01180 0.77 +0.8 262,379 + JOY50_RS01180 0.77 +0.3 262,406 - JOY50_RS01180 0.79 -0.2 262,408 - JOY50_RS01180 0.79 -0.5 262,408 - JOY50_RS01180 0.79 -0.6 262,513 + JOY50_RS01180 0.86 -0.3 262,898 - -1.1 262,919 - +0.5 263,024 - JOY50_RS01185 0.17 -1.0 263,024 - JOY50_RS01185 0.17 -0.7 263,029 - JOY50_RS01185 0.18 -2.1 263,029 - JOY50_RS01185 0.18 -1.8 263,077 - JOY50_RS01185 0.23 -0.1 263,104 - JOY50_RS01185 0.26 +0.4 263,124 - JOY50_RS01185 0.28 -0.8 263,135 - JOY50_RS01185 0.30 +0.1 263,174 - JOY50_RS01185 0.34 -1.1 263,191 + JOY50_RS01185 0.36 -0.1 263,204 - JOY50_RS01185 0.37 -0.2 263,204 - JOY50_RS01185 0.37 +0.3 263,206 + JOY50_RS01185 0.37 +0.3 263,219 - JOY50_RS01185 0.39 -0.7 263,219 - JOY50_RS01185 0.39 +0.6 263,219 - JOY50_RS01185 0.39 -0.4 263,219 - JOY50_RS01185 0.39 +1.2 263,254 - JOY50_RS01185 0.43 +0.9 263,256 - JOY50_RS01185 0.43 +0.3 263,276 - JOY50_RS01185 0.45 +0.9 263,288 - JOY50_RS01185 0.47 +0.0 263,288 - JOY50_RS01185 0.47 -0.5 263,335 - JOY50_RS01185 0.52 +0.5 263,345 - JOY50_RS01185 0.53 +0.8 263,365 - JOY50_RS01185 0.55 +0.3 263,414 - JOY50_RS01185 0.61 -0.4 263,453 - JOY50_RS01185 0.65 +0.8 263,469 - JOY50_RS01185 0.67 -0.6 263,469 - JOY50_RS01185 0.67 -0.4 263,491 - JOY50_RS01185 0.69 -1.3 263,498 - JOY50_RS01185 0.70 -0.3 263,498 - JOY50_RS01185 0.70 -0.7 263,500 - JOY50_RS01185 0.70 -0.8 263,500 - JOY50_RS01185 0.70 +0.2 263,520 - JOY50_RS01185 0.73 -0.2 263,525 - JOY50_RS01185 0.73 -0.3 263,552 - JOY50_RS01185 0.76 +0.5 263,589 - JOY50_RS01185 0.80 +0.3 263,590 - JOY50_RS01185 0.80 +0.6 263,652 - JOY50_RS01185 0.87 +0.1 263,652 - JOY50_RS01185 0.87 +1.2
Or see this region's nucleotide sequence