Experiment: .-Glucan from yeast 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS08325 and HMPREF1078_RS19570 are separated by 340 nucleotides HMPREF1078_RS19570 and HMPREF1078_RS19575 are separated by 4 nucleotides HMPREF1078_RS19575 and HMPREF1078_RS08340 are separated by 31 nucleotides HMPREF1078_RS08340 and HMPREF1078_RS19580 are separated by 323 nucleotides HMPREF1078_RS19580 and HMPREF1078_RS08355 are separated by 370 nucleotides
HMPREF1078_RS08325: HMPREF1078_RS08325 - helix-turn-helix domain-containing protein, at 236,161 to 236,484
_RS08325
HMPREF1078_RS19570: HMPREF1078_RS19570 - hypothetical protein, at 236,825 to 237,004
_RS19570
HMPREF1078_RS19575: HMPREF1078_RS19575 - hypothetical protein, at 237,009 to 237,401
_RS19575
HMPREF1078_RS08340: HMPREF1078_RS08340 - sigma-70 family RNA polymerase sigma factor, at 237,433 to 237,993
_RS08340
HMPREF1078_RS19580: HMPREF1078_RS19580 - hypothetical protein, at 238,317 to 238,568
_RS19580
HMPREF1078_RS08355: HMPREF1078_RS08355 - GNAT family N-acetyltransferase, at 238,939 to 239,538
_RS08355
Position (kb) 237
238 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 236.980 kb on - strand, within HMPREF1078_RS19570 at 236.982 kb on - strand, within HMPREF1078_RS19570 at 237.198 kb on - strand, within HMPREF1078_RS19575 at 237.217 kb on + strand, within HMPREF1078_RS19575 at 237.218 kb on - strand, within HMPREF1078_RS19575 at 237.385 kb on + strand at 237.385 kb on + strand at 237.385 kb on + strand at 237.386 kb on + strand at 237.387 kb on + strand at 237.438 kb on - strand at 237.935 kb on + strand, within HMPREF1078_RS08340 at 237.935 kb on + strand, within HMPREF1078_RS08340 at 237.993 kb on + strand at 237.993 kb on + strand at 238.017 kb on - strand at 238.017 kb on - strand at 238.017 kb on - strand at 238.232 kb on + strand at 238.258 kb on + strand at 238.287 kb on + strand at 238.311 kb on + strand at 238.330 kb on + strand at 238.330 kb on + strand at 238.331 kb on - strand at 238.331 kb on - strand at 238.400 kb on - strand, within HMPREF1078_RS19580 at 238.402 kb on - strand, within HMPREF1078_RS19580 at 238.470 kb on + strand, within HMPREF1078_RS19580 at 238.471 kb on - strand, within HMPREF1078_RS19580 at 238.471 kb on - strand, within HMPREF1078_RS19580 at 238.477 kb on - strand, within HMPREF1078_RS19580 at 238.477 kb on - strand, within HMPREF1078_RS19580 at 238.477 kb on - strand, within HMPREF1078_RS19580 at 238.477 kb on - strand, within HMPREF1078_RS19580 at 238.585 kb on + strand at 238.586 kb on - strand at 238.589 kb on + strand at 238.589 kb on + strand at 238.590 kb on - strand at 238.600 kb on + strand at 238.600 kb on + strand at 238.601 kb on - strand at 238.601 kb on - strand at 238.614 kb on + strand at 238.614 kb on + strand at 238.615 kb on - strand at 238.615 kb on - strand at 238.615 kb on - strand at 238.667 kb on + strand at 238.668 kb on - strand at 238.677 kb on + strand at 238.682 kb on + strand at 238.765 kb on - strand at 238.765 kb on - strand at 238.772 kb on + strand at 238.773 kb on - strand at 238.773 kb on - strand at 238.795 kb on - strand at 238.795 kb on - strand at 238.861 kb on + strand at 238.870 kb on + strand at 238.927 kb on + strand at 238.928 kb on - strand at 238.928 kb on - strand at 238.929 kb on + strand at 238.929 kb on + strand at 238.930 kb on - strand at 238.930 kb on - strand at 238.930 kb on - strand at 238.930 kb on - strand at 238.930 kb on - strand at 238.930 kb on - strand at 238.933 kb on - strand at 238.933 kb on - strand at 238.933 kb on - strand at 238.933 kb on - strand at 238.935 kb on + strand at 238.935 kb on + strand at 238.936 kb on - strand at 238.936 kb on - strand at 238.962 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction .-Glucan from yeast 2.5 mg/mL (C) remove 236,980 - HMPREF1078_RS19570 0.86 -0.4 236,982 - HMPREF1078_RS19570 0.87 +1.0 237,198 - HMPREF1078_RS19575 0.48 -1.4 237,217 + HMPREF1078_RS19575 0.53 -1.5 237,218 - HMPREF1078_RS19575 0.53 +0.8 237,385 + +1.1 237,385 + -0.4 237,385 + -1.2 237,386 + +0.5 237,387 + +0.1 237,438 - +0.9 237,935 + HMPREF1078_RS08340 0.89 +1.8 237,935 + HMPREF1078_RS08340 0.89 +1.6 237,993 + +1.3 237,993 + +0.4 238,017 - +1.5 238,017 - -2.6 238,017 - -3.4 238,232 + -1.6 238,258 + -0.6 238,287 + +1.4 238,311 + -0.4 238,330 + +1.3 238,330 + +0.6 238,331 - +0.1 238,331 - +0.4 238,400 - HMPREF1078_RS19580 0.33 +0.6 238,402 - HMPREF1078_RS19580 0.34 +0.8 238,470 + HMPREF1078_RS19580 0.61 -0.5 238,471 - HMPREF1078_RS19580 0.61 +0.3 238,471 - HMPREF1078_RS19580 0.61 +1.6 238,477 - HMPREF1078_RS19580 0.63 +0.4 238,477 - HMPREF1078_RS19580 0.63 -2.9 238,477 - HMPREF1078_RS19580 0.63 -1.1 238,477 - HMPREF1078_RS19580 0.63 +1.4 238,585 + +0.5 238,586 - +0.9 238,589 + -0.3 238,589 + -0.4 238,590 - +3.5 238,600 + +2.0 238,600 + +0.7 238,601 - +1.0 238,601 - -1.3 238,614 + +0.5 238,614 + -1.3 238,615 - -2.2 238,615 - -0.7 238,615 - +1.5 238,667 + -2.2 238,668 - -0.7 238,677 + +1.9 238,682 + -2.5 238,765 - -0.9 238,765 - -0.8 238,772 + +0.4 238,773 - +1.2 238,773 - +0.3 238,795 - +0.9 238,795 - -0.9 238,861 + -0.1 238,870 + -3.1 238,927 + -2.2 238,928 - +0.6 238,928 - -0.9 238,929 + -0.8 238,929 + -0.8 238,930 - +0.3 238,930 - -2.1 238,930 - +0.7 238,930 - +0.3 238,930 - -0.6 238,930 - +0.7 238,933 - -2.3 238,933 - -0.3 238,933 - +1.9 238,933 - +0.9 238,935 + +0.9 238,935 + +0.8 238,936 - -2.9 238,936 - -3.2 238,962 - +1.7
Or see this region's nucleotide sequence