Experiment: .-Glucan from yeast 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS05980 and HMPREF1078_RS05985 are separated by 419 nucleotides HMPREF1078_RS05985 and HMPREF1078_RS05990 are separated by 145 nucleotides
HMPREF1078_RS05980: HMPREF1078_RS05980 - TolC family protein, at 534,632 to 535,993
_RS05980
HMPREF1078_RS05985: HMPREF1078_RS05985 - flavin reductase family protein, at 536,413 to 536,919
_RS05985
HMPREF1078_RS05990: HMPREF1078_RS05990 - alanine/glycine:cation symporter family protein, at 537,065 to 538,465
_RS05990
Position (kb)
536
537 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 535.453 kb on - strand, within HMPREF1078_RS05980 at 535.526 kb on + strand, within HMPREF1078_RS05980 at 535.832 kb on - strand, within HMPREF1078_RS05980 at 535.857 kb on + strand at 536.199 kb on - strand at 536.199 kb on - strand at 536.226 kb on - strand at 536.245 kb on + strand at 536.250 kb on - strand at 536.260 kb on + strand at 536.261 kb on - strand at 536.261 kb on - strand at 536.264 kb on + strand at 536.301 kb on + strand at 536.301 kb on + strand at 536.305 kb on + strand at 536.306 kb on - strand at 536.306 kb on - strand at 536.316 kb on + strand at 536.317 kb on - strand at 536.381 kb on + strand at 536.432 kb on - strand at 536.447 kb on + strand at 536.492 kb on + strand, within HMPREF1078_RS05985 at 536.520 kb on + strand, within HMPREF1078_RS05985 at 536.529 kb on + strand, within HMPREF1078_RS05985 at 536.604 kb on - strand, within HMPREF1078_RS05985 at 536.740 kb on - strand, within HMPREF1078_RS05985 at 536.740 kb on - strand, within HMPREF1078_RS05985 at 536.741 kb on + strand, within HMPREF1078_RS05985 at 536.765 kb on - strand, within HMPREF1078_RS05985 at 536.795 kb on - strand, within HMPREF1078_RS05985 at 536.795 kb on - strand, within HMPREF1078_RS05985 at 536.795 kb on - strand, within HMPREF1078_RS05985 at 536.795 kb on - strand, within HMPREF1078_RS05985 at 536.849 kb on - strand, within HMPREF1078_RS05985 at 536.908 kb on + strand at 536.909 kb on - strand at 536.919 kb on + strand at 536.920 kb on - strand at 536.933 kb on - strand at 536.936 kb on + strand at 536.940 kb on - strand at 536.961 kb on + strand at 536.988 kb on - strand at 537.000 kb on - strand at 537.039 kb on + strand at 537.040 kb on - strand at 537.040 kb on - strand at 537.040 kb on - strand at 537.076 kb on + strand at 537.138 kb on + strand at 537.204 kb on + strand at 537.204 kb on + strand at 537.412 kb on + strand, within HMPREF1078_RS05990 at 537.428 kb on + strand, within HMPREF1078_RS05990 at 537.470 kb on + strand, within HMPREF1078_RS05990 at 537.473 kb on + strand, within HMPREF1078_RS05990 at 537.473 kb on + strand, within HMPREF1078_RS05990 at 537.476 kb on - strand, within HMPREF1078_RS05990 at 537.476 kb on - strand, within HMPREF1078_RS05990 at 537.510 kb on + strand, within HMPREF1078_RS05990 at 537.510 kb on + strand, within HMPREF1078_RS05990 at 537.511 kb on - strand, within HMPREF1078_RS05990 at 537.511 kb on - strand, within HMPREF1078_RS05990 at 537.511 kb on - strand, within HMPREF1078_RS05990 at 537.511 kb on - strand, within HMPREF1078_RS05990 at 537.511 kb on - strand, within HMPREF1078_RS05990 at 537.622 kb on - strand, within HMPREF1078_RS05990 at 537.678 kb on + strand, within HMPREF1078_RS05990 at 537.747 kb on + strand, within HMPREF1078_RS05990 at 537.747 kb on + strand, within HMPREF1078_RS05990 at 537.759 kb on + strand, within HMPREF1078_RS05990 at 537.760 kb on - strand, within HMPREF1078_RS05990 at 537.793 kb on - strand, within HMPREF1078_RS05990
Per-strain Table
Position Strand Gene LocusTag Fraction .-Glucan from yeast 2.5 mg/mL (C) remove 535,453 - HMPREF1078_RS05980 0.60 -2.8 535,526 + HMPREF1078_RS05980 0.66 -0.8 535,832 - HMPREF1078_RS05980 0.88 -1.6 535,857 + -3.0 536,199 - -0.6 536,199 - -1.1 536,226 - -0.2 536,245 + -0.1 536,250 - -0.2 536,260 + -0.2 536,261 - +0.9 536,261 - -0.3 536,264 + -2.1 536,301 + +1.2 536,301 + +0.3 536,305 + -0.8 536,306 - -2.3 536,306 - -2.0 536,316 + +1.0 536,317 - +0.4 536,381 + +0.6 536,432 - -1.3 536,447 + -0.9 536,492 + HMPREF1078_RS05985 0.16 +0.6 536,520 + HMPREF1078_RS05985 0.21 -0.1 536,529 + HMPREF1078_RS05985 0.23 -0.0 536,604 - HMPREF1078_RS05985 0.38 -2.5 536,740 - HMPREF1078_RS05985 0.64 +0.8 536,740 - HMPREF1078_RS05985 0.64 +0.7 536,741 + HMPREF1078_RS05985 0.65 +0.4 536,765 - HMPREF1078_RS05985 0.69 +2.0 536,795 - HMPREF1078_RS05985 0.75 -2.7 536,795 - HMPREF1078_RS05985 0.75 +1.3 536,795 - HMPREF1078_RS05985 0.75 -1.1 536,795 - HMPREF1078_RS05985 0.75 +0.2 536,849 - HMPREF1078_RS05985 0.86 -0.5 536,908 + -0.0 536,909 - -0.5 536,919 + +0.6 536,920 - -1.4 536,933 - -0.5 536,936 + +0.5 536,940 - -0.0 536,961 + +0.1 536,988 - +0.9 537,000 - +2.0 537,039 + +0.2 537,040 - -0.3 537,040 - -1.4 537,040 - +0.3 537,076 + -1.7 537,138 + -0.7 537,204 + -1.2 537,204 + -0.7 537,412 + HMPREF1078_RS05990 0.25 -0.1 537,428 + HMPREF1078_RS05990 0.26 -1.7 537,470 + HMPREF1078_RS05990 0.29 -1.5 537,473 + HMPREF1078_RS05990 0.29 +1.4 537,473 + HMPREF1078_RS05990 0.29 +1.1 537,476 - HMPREF1078_RS05990 0.29 -0.0 537,476 - HMPREF1078_RS05990 0.29 +1.0 537,510 + HMPREF1078_RS05990 0.32 +2.3 537,510 + HMPREF1078_RS05990 0.32 +0.4 537,511 - HMPREF1078_RS05990 0.32 +0.2 537,511 - HMPREF1078_RS05990 0.32 -0.6 537,511 - HMPREF1078_RS05990 0.32 -0.7 537,511 - HMPREF1078_RS05990 0.32 -0.7 537,511 - HMPREF1078_RS05990 0.32 -0.8 537,622 - HMPREF1078_RS05990 0.40 +0.5 537,678 + HMPREF1078_RS05990 0.44 -0.1 537,747 + HMPREF1078_RS05990 0.49 +1.7 537,747 + HMPREF1078_RS05990 0.49 +1.9 537,759 + HMPREF1078_RS05990 0.50 +0.7 537,760 - HMPREF1078_RS05990 0.50 -0.6 537,793 - HMPREF1078_RS05990 0.52 +0.0
Or see this region's nucleotide sequence