Experiment: Lentilan from mushroom 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS13555 and HMPREF1078_RS13560 are separated by 5 nucleotides HMPREF1078_RS13560 and HMPREF1078_RS13565 are separated by 155 nucleotides HMPREF1078_RS13565 and HMPREF1078_RS13570 are separated by 18 nucleotides HMPREF1078_RS13570 and HMPREF1078_RS13575 are separated by 6 nucleotides
HMPREF1078_RS13555: HMPREF1078_RS13555 - ATP-binding cassette domain-containing protein, at 158,337 to 159,014
_RS13555
HMPREF1078_RS13560: HMPREF1078_RS13560 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 159,020 to 159,616
_RS13560
HMPREF1078_RS13565: HMPREF1078_RS13565 - imidazole glycerol phosphate synthase subunit HisF, at 159,772 to 160,527
_RS13565
HMPREF1078_RS13570: HMPREF1078_RS13570 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 160,546 to 161,265
_RS13570
HMPREF1078_RS13575: HMPREF1078_RS13575 - imidazole glycerol phosphate synthase subunit HisH, at 161,272 to 161,862
_RS13575
Position (kb) 159
160
161 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 158.810 kb on + strand, within HMPREF1078_RS13555 at 158.812 kb on + strand, within HMPREF1078_RS13555 at 158.813 kb on - strand, within HMPREF1078_RS13555 at 158.813 kb on - strand, within HMPREF1078_RS13555 at 158.813 kb on - strand, within HMPREF1078_RS13555 at 158.813 kb on - strand, within HMPREF1078_RS13555 at 158.934 kb on - strand, within HMPREF1078_RS13555 at 158.975 kb on + strand at 158.976 kb on - strand at 158.976 kb on - strand at 158.979 kb on + strand at 158.979 kb on + strand at 159.019 kb on - strand at 159.019 kb on - strand at 159.029 kb on - strand at 159.089 kb on + strand, within HMPREF1078_RS13560 at 159.089 kb on + strand, within HMPREF1078_RS13560 at 159.185 kb on + strand, within HMPREF1078_RS13560 at 159.200 kb on - strand, within HMPREF1078_RS13560 at 159.234 kb on + strand, within HMPREF1078_RS13560 at 159.286 kb on - strand, within HMPREF1078_RS13560 at 159.286 kb on - strand, within HMPREF1078_RS13560 at 159.331 kb on + strand, within HMPREF1078_RS13560 at 159.331 kb on + strand, within HMPREF1078_RS13560 at 159.331 kb on + strand, within HMPREF1078_RS13560 at 159.331 kb on + strand, within HMPREF1078_RS13560 at 159.332 kb on - strand, within HMPREF1078_RS13560 at 159.529 kb on + strand, within HMPREF1078_RS13560 at 159.632 kb on + strand at 159.632 kb on + strand at 159.632 kb on + strand at 159.633 kb on - strand at 159.633 kb on - strand at 159.633 kb on - strand at 159.634 kb on + strand at 159.635 kb on - strand at 159.635 kb on - strand at 159.644 kb on - strand at 159.655 kb on - strand at 159.692 kb on + strand at 159.693 kb on - strand at 159.706 kb on + strand at 159.707 kb on - strand at 159.807 kb on - strand at 159.888 kb on - strand, within HMPREF1078_RS13565 at 159.963 kb on + strand, within HMPREF1078_RS13565 at 159.963 kb on + strand, within HMPREF1078_RS13565 at 160.156 kb on + strand, within HMPREF1078_RS13565 at 160.156 kb on + strand, within HMPREF1078_RS13565 at 160.157 kb on - strand, within HMPREF1078_RS13565 at 160.350 kb on + strand, within HMPREF1078_RS13565 at 160.373 kb on - strand, within HMPREF1078_RS13565 at 160.373 kb on - strand, within HMPREF1078_RS13565 at 160.375 kb on - strand, within HMPREF1078_RS13565 at 160.383 kb on - strand, within HMPREF1078_RS13565 at 160.383 kb on - strand, within HMPREF1078_RS13565 at 160.384 kb on + strand, within HMPREF1078_RS13565 at 160.385 kb on - strand, within HMPREF1078_RS13565 at 160.412 kb on + strand, within HMPREF1078_RS13565 at 160.413 kb on - strand, within HMPREF1078_RS13565 at 160.493 kb on - strand at 160.510 kb on + strand at 160.511 kb on - strand at 160.511 kb on - strand at 160.511 kb on - strand at 160.511 kb on - strand at 160.527 kb on + strand at 160.527 kb on + strand at 160.528 kb on - strand at 160.528 kb on - strand at 160.528 kb on - strand at 160.530 kb on - strand at 160.531 kb on + strand at 160.531 kb on + strand at 160.539 kb on - strand at 160.544 kb on + strand at 160.545 kb on - strand at 160.576 kb on + strand at 160.645 kb on - strand, within HMPREF1078_RS13570 at 160.645 kb on - strand, within HMPREF1078_RS13570 at 160.651 kb on + strand, within HMPREF1078_RS13570 at 160.652 kb on - strand, within HMPREF1078_RS13570 at 160.653 kb on + strand, within HMPREF1078_RS13570 at 160.653 kb on + strand, within HMPREF1078_RS13570 at 160.663 kb on - strand, within HMPREF1078_RS13570 at 160.663 kb on - strand, within HMPREF1078_RS13570 at 160.693 kb on + strand, within HMPREF1078_RS13570 at 160.694 kb on - strand, within HMPREF1078_RS13570 at 160.694 kb on - strand, within HMPREF1078_RS13570 at 160.705 kb on + strand, within HMPREF1078_RS13570 at 160.713 kb on + strand, within HMPREF1078_RS13570 at 160.716 kb on - strand, within HMPREF1078_RS13570 at 160.717 kb on + strand, within HMPREF1078_RS13570 at 160.717 kb on + strand, within HMPREF1078_RS13570 at 160.717 kb on + strand, within HMPREF1078_RS13570 at 160.718 kb on - strand, within HMPREF1078_RS13570 at 160.718 kb on - strand, within HMPREF1078_RS13570 at 160.743 kb on + strand, within HMPREF1078_RS13570 at 160.744 kb on - strand, within HMPREF1078_RS13570 at 160.783 kb on - strand, within HMPREF1078_RS13570 at 160.797 kb on - strand, within HMPREF1078_RS13570 at 160.827 kb on - strand, within HMPREF1078_RS13570 at 160.928 kb on - strand, within HMPREF1078_RS13570 at 161.047 kb on - strand, within HMPREF1078_RS13570 at 161.047 kb on - strand, within HMPREF1078_RS13570 at 161.070 kb on + strand, within HMPREF1078_RS13570 at 161.070 kb on + strand, within HMPREF1078_RS13570 at 161.075 kb on + strand, within HMPREF1078_RS13570 at 161.075 kb on + strand, within HMPREF1078_RS13570 at 161.076 kb on - strand, within HMPREF1078_RS13570 at 161.076 kb on - strand, within HMPREF1078_RS13570 at 161.076 kb on - strand, within HMPREF1078_RS13570 at 161.076 kb on - strand, within HMPREF1078_RS13570 at 161.076 kb on - strand, within HMPREF1078_RS13570 at 161.198 kb on + strand at 161.198 kb on + strand at 161.199 kb on - strand at 161.199 kb on - strand at 161.219 kb on - strand at 161.238 kb on + strand at 161.239 kb on - strand at 161.239 kb on - strand at 161.242 kb on + strand at 161.242 kb on + strand at 161.242 kb on + strand at 161.242 kb on + strand at 161.243 kb on - strand at 161.243 kb on - strand at 161.265 kb on + strand at 161.266 kb on - strand at 161.268 kb on + strand at 161.269 kb on - strand at 161.274 kb on - strand at 161.293 kb on + strand at 161.294 kb on - strand at 161.296 kb on + strand at 161.297 kb on - strand at 161.297 kb on - strand at 161.298 kb on + strand at 161.299 kb on - strand at 161.299 kb on - strand at 161.385 kb on + strand, within HMPREF1078_RS13575 at 161.386 kb on - strand, within HMPREF1078_RS13575 at 161.386 kb on - strand, within HMPREF1078_RS13575 at 161.387 kb on + strand, within HMPREF1078_RS13575 at 161.388 kb on - strand, within HMPREF1078_RS13575 at 161.422 kb on + strand, within HMPREF1078_RS13575 at 161.426 kb on + strand, within HMPREF1078_RS13575 at 161.426 kb on + strand, within HMPREF1078_RS13575 at 161.426 kb on + strand, within HMPREF1078_RS13575 at 161.427 kb on - strand, within HMPREF1078_RS13575 at 161.427 kb on - strand, within HMPREF1078_RS13575 at 161.427 kb on - strand, within HMPREF1078_RS13575 at 161.429 kb on + strand, within HMPREF1078_RS13575 at 161.430 kb on - strand, within HMPREF1078_RS13575 at 161.430 kb on - strand, within HMPREF1078_RS13575 at 161.430 kb on - strand, within HMPREF1078_RS13575 at 161.433 kb on + strand, within HMPREF1078_RS13575 at 161.433 kb on + strand, within HMPREF1078_RS13575 at 161.433 kb on + strand, within HMPREF1078_RS13575 at 161.433 kb on + strand, within HMPREF1078_RS13575 at 161.438 kb on - strand, within HMPREF1078_RS13575 at 161.497 kb on - strand, within HMPREF1078_RS13575 at 161.526 kb on + strand, within HMPREF1078_RS13575
Per-strain Table
Position Strand Gene LocusTag Fraction Lentilan from mushroom 2.5 mg/mL (C) remove 158,810 + HMPREF1078_RS13555 0.70 +0.1 158,812 + HMPREF1078_RS13555 0.70 -1.1 158,813 - HMPREF1078_RS13555 0.70 -1.3 158,813 - HMPREF1078_RS13555 0.70 +1.1 158,813 - HMPREF1078_RS13555 0.70 +0.4 158,813 - HMPREF1078_RS13555 0.70 -0.5 158,934 - HMPREF1078_RS13555 0.88 -1.1 158,975 + -0.8 158,976 - +0.2 158,976 - +0.5 158,979 + -1.2 158,979 + +0.8 159,019 - -1.9 159,019 - -1.5 159,029 - +0.5 159,089 + HMPREF1078_RS13560 0.12 -1.1 159,089 + HMPREF1078_RS13560 0.12 +0.1 159,185 + HMPREF1078_RS13560 0.28 -0.7 159,200 - HMPREF1078_RS13560 0.30 -1.5 159,234 + HMPREF1078_RS13560 0.36 +0.4 159,286 - HMPREF1078_RS13560 0.45 -0.1 159,286 - HMPREF1078_RS13560 0.45 -0.5 159,331 + HMPREF1078_RS13560 0.52 -0.5 159,331 + HMPREF1078_RS13560 0.52 -2.7 159,331 + HMPREF1078_RS13560 0.52 -2.1 159,331 + HMPREF1078_RS13560 0.52 +0.1 159,332 - HMPREF1078_RS13560 0.52 -0.3 159,529 + HMPREF1078_RS13560 0.85 -0.7 159,632 + -2.0 159,632 + -1.4 159,632 + -1.1 159,633 - +0.7 159,633 - +0.0 159,633 - +2.5 159,634 + -0.2 159,635 - +0.2 159,635 - -0.8 159,644 - +0.3 159,655 - -0.0 159,692 + -1.5 159,693 - +0.5 159,706 + -2.0 159,707 - -0.7 159,807 - -0.3 159,888 - HMPREF1078_RS13565 0.15 -3.3 159,963 + HMPREF1078_RS13565 0.25 -0.4 159,963 + HMPREF1078_RS13565 0.25 -1.3 160,156 + HMPREF1078_RS13565 0.51 -1.0 160,156 + HMPREF1078_RS13565 0.51 -1.2 160,157 - HMPREF1078_RS13565 0.51 -1.1 160,350 + HMPREF1078_RS13565 0.76 -0.2 160,373 - HMPREF1078_RS13565 0.79 -1.4 160,373 - HMPREF1078_RS13565 0.79 -1.8 160,375 - HMPREF1078_RS13565 0.80 -2.0 160,383 - HMPREF1078_RS13565 0.81 -0.6 160,383 - HMPREF1078_RS13565 0.81 -3.4 160,384 + HMPREF1078_RS13565 0.81 +0.4 160,385 - HMPREF1078_RS13565 0.81 +0.1 160,412 + HMPREF1078_RS13565 0.85 -0.5 160,413 - HMPREF1078_RS13565 0.85 -3.0 160,493 - -1.8 160,510 + -0.9 160,511 - +0.7 160,511 - -1.0 160,511 - -1.6 160,511 - -0.9 160,527 + -1.4 160,527 + -2.0 160,528 - +0.8 160,528 - -0.4 160,528 - +0.3 160,530 - +0.1 160,531 + -1.0 160,531 + -1.6 160,539 - +0.1 160,544 + -0.2 160,545 - +1.7 160,576 + -1.2 160,645 - HMPREF1078_RS13570 0.14 -1.1 160,645 - HMPREF1078_RS13570 0.14 -0.6 160,651 + HMPREF1078_RS13570 0.15 +0.8 160,652 - HMPREF1078_RS13570 0.15 -0.7 160,653 + HMPREF1078_RS13570 0.15 +0.5 160,653 + HMPREF1078_RS13570 0.15 -0.3 160,663 - HMPREF1078_RS13570 0.16 -1.5 160,663 - HMPREF1078_RS13570 0.16 -0.6 160,693 + HMPREF1078_RS13570 0.20 -0.8 160,694 - HMPREF1078_RS13570 0.21 -2.2 160,694 - HMPREF1078_RS13570 0.21 -4.1 160,705 + HMPREF1078_RS13570 0.22 -1.2 160,713 + HMPREF1078_RS13570 0.23 -1.3 160,716 - HMPREF1078_RS13570 0.24 -1.7 160,717 + HMPREF1078_RS13570 0.24 -1.3 160,717 + HMPREF1078_RS13570 0.24 -1.2 160,717 + HMPREF1078_RS13570 0.24 -3.0 160,718 - HMPREF1078_RS13570 0.24 -0.8 160,718 - HMPREF1078_RS13570 0.24 -2.9 160,743 + HMPREF1078_RS13570 0.27 -1.2 160,744 - HMPREF1078_RS13570 0.28 -1.3 160,783 - HMPREF1078_RS13570 0.33 -0.8 160,797 - HMPREF1078_RS13570 0.35 -1.0 160,827 - HMPREF1078_RS13570 0.39 -0.1 160,928 - HMPREF1078_RS13570 0.53 -1.6 161,047 - HMPREF1078_RS13570 0.70 -0.9 161,047 - HMPREF1078_RS13570 0.70 -1.1 161,070 + HMPREF1078_RS13570 0.73 -0.4 161,070 + HMPREF1078_RS13570 0.73 -2.1 161,075 + HMPREF1078_RS13570 0.73 -0.8 161,075 + HMPREF1078_RS13570 0.73 -0.8 161,076 - HMPREF1078_RS13570 0.74 -0.8 161,076 - HMPREF1078_RS13570 0.74 +0.1 161,076 - HMPREF1078_RS13570 0.74 -1.6 161,076 - HMPREF1078_RS13570 0.74 -1.4 161,076 - HMPREF1078_RS13570 0.74 -1.1 161,198 + -0.5 161,198 + -0.6 161,199 - -0.7 161,199 - +0.2 161,219 - -1.0 161,238 + -0.5 161,239 - +1.2 161,239 - -1.4 161,242 + -1.7 161,242 + -0.8 161,242 + -1.4 161,242 + -0.9 161,243 - -0.2 161,243 - -1.1 161,265 + -2.1 161,266 - +1.1 161,268 + -2.3 161,269 - -0.4 161,274 - -0.1 161,293 + +0.7 161,294 - -1.0 161,296 + -1.9 161,297 - +0.7 161,297 - -2.0 161,298 + -1.3 161,299 - +0.5 161,299 - +0.2 161,385 + HMPREF1078_RS13575 0.19 +0.4 161,386 - HMPREF1078_RS13575 0.19 +0.4 161,386 - HMPREF1078_RS13575 0.19 -0.2 161,387 + HMPREF1078_RS13575 0.19 -2.2 161,388 - HMPREF1078_RS13575 0.20 +1.0 161,422 + HMPREF1078_RS13575 0.25 -0.6 161,426 + HMPREF1078_RS13575 0.26 -1.1 161,426 + HMPREF1078_RS13575 0.26 -0.9 161,426 + HMPREF1078_RS13575 0.26 -0.5 161,427 - HMPREF1078_RS13575 0.26 +0.8 161,427 - HMPREF1078_RS13575 0.26 -1.3 161,427 - HMPREF1078_RS13575 0.26 -0.3 161,429 + HMPREF1078_RS13575 0.27 -1.4 161,430 - HMPREF1078_RS13575 0.27 +0.5 161,430 - HMPREF1078_RS13575 0.27 -2.1 161,430 - HMPREF1078_RS13575 0.27 -0.6 161,433 + HMPREF1078_RS13575 0.27 -1.2 161,433 + HMPREF1078_RS13575 0.27 -2.2 161,433 + HMPREF1078_RS13575 0.27 -1.8 161,433 + HMPREF1078_RS13575 0.27 -1.2 161,438 - HMPREF1078_RS13575 0.28 -0.6 161,497 - HMPREF1078_RS13575 0.38 +0.1 161,526 + HMPREF1078_RS13575 0.43 -1.6
Or see this region's nucleotide sequence