Experiment: Dextrin from potato starch 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS08320 and HMPREF1078_RS08325 are separated by 43 nucleotides HMPREF1078_RS08325 and HMPREF1078_RS19570 are separated by 340 nucleotides HMPREF1078_RS19570 and HMPREF1078_RS19575 are separated by 4 nucleotides HMPREF1078_RS19575 and HMPREF1078_RS08340 are separated by 31 nucleotides
HMPREF1078_RS08320: HMPREF1078_RS08320 - helix-turn-helix domain-containing protein, at 235,806 to 236,117
_RS08320
HMPREF1078_RS08325: HMPREF1078_RS08325 - helix-turn-helix domain-containing protein, at 236,161 to 236,484
_RS08325
HMPREF1078_RS19570: HMPREF1078_RS19570 - hypothetical protein, at 236,825 to 237,004
_RS19570
HMPREF1078_RS19575: HMPREF1078_RS19575 - hypothetical protein, at 237,009 to 237,401
_RS19575
HMPREF1078_RS08340: HMPREF1078_RS08340 - sigma-70 family RNA polymerase sigma factor, at 237,433 to 237,993
_RS08340
Position (kb)
236
237
238 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 236.980 kb on - strand, within HMPREF1078_RS19570 at 236.982 kb on - strand, within HMPREF1078_RS19570 at 237.198 kb on - strand, within HMPREF1078_RS19575 at 237.217 kb on + strand, within HMPREF1078_RS19575 at 237.218 kb on - strand, within HMPREF1078_RS19575 at 237.385 kb on + strand at 237.385 kb on + strand at 237.385 kb on + strand at 237.386 kb on + strand at 237.387 kb on + strand at 237.438 kb on - strand at 237.935 kb on + strand, within HMPREF1078_RS08340 at 237.935 kb on + strand, within HMPREF1078_RS08340 at 237.993 kb on + strand at 237.993 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dextrin from potato starch 2.5 mg/mL (C) remove 236,980 - HMPREF1078_RS19570 0.86 -2.0 236,982 - HMPREF1078_RS19570 0.87 -2.0 237,198 - HMPREF1078_RS19575 0.48 +1.9 237,217 + HMPREF1078_RS19575 0.53 -2.5 237,218 - HMPREF1078_RS19575 0.53 -0.1 237,385 + +1.1 237,385 + +0.6 237,385 + -1.2 237,386 + -0.2 237,387 + +0.2 237,438 - +1.3 237,935 + HMPREF1078_RS08340 0.89 +0.8 237,935 + HMPREF1078_RS08340 0.89 +0.3 237,993 + +0.8 237,993 + +1.4
Or see this region's nucleotide sequence