Experiment: Dextrin from potato starch 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS04795 and HMPREF1078_RS04800 are separated by 9 nucleotides HMPREF1078_RS04800 and HMPREF1078_RS04805 are separated by 72 nucleotides HMPREF1078_RS04805 and HMPREF1078_RS04810 are separated by 4 nucleotides HMPREF1078_RS04810 and HMPREF1078_RS04815 are separated by 199 nucleotides
HMPREF1078_RS04795: HMPREF1078_RS04795 - CDP-alcohol phosphatidyltransferase family protein, at 207,338 to 208,042
_RS04795
HMPREF1078_RS04800: HMPREF1078_RS04800 - phosphatidylserine decarboxylase family protein, at 208,052 to 208,714
_RS04800
HMPREF1078_RS04805: HMPREF1078_RS04805 - UvrD-helicase domain-containing protein, at 208,787 to 212,014
_RS04805
HMPREF1078_RS04810: HMPREF1078_RS04810 - translation initiation factor, at 212,019 to 212,363
_RS04810
HMPREF1078_RS04815: HMPREF1078_RS04815 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 212,563 to 213,039
_RS04815
Position (kb)
208
209
210
211
212
213 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 208.733 kb on - strand at 208.805 kb on + strand at 208.814 kb on + strand at 208.814 kb on + strand at 208.824 kb on + strand at 208.825 kb on - strand at 208.825 kb on - strand at 208.887 kb on - strand at 208.894 kb on + strand at 208.895 kb on - strand at 208.895 kb on - strand at 208.895 kb on - strand at 208.895 kb on - strand at 208.927 kb on + strand at 208.928 kb on - strand at 209.171 kb on + strand, within HMPREF1078_RS04805 at 209.252 kb on + strand, within HMPREF1078_RS04805 at 209.253 kb on - strand, within HMPREF1078_RS04805 at 209.284 kb on + strand, within HMPREF1078_RS04805 at 209.422 kb on + strand, within HMPREF1078_RS04805 at 209.554 kb on + strand, within HMPREF1078_RS04805 at 209.566 kb on - strand, within HMPREF1078_RS04805 at 209.566 kb on - strand, within HMPREF1078_RS04805 at 209.568 kb on - strand, within HMPREF1078_RS04805 at 209.766 kb on - strand, within HMPREF1078_RS04805 at 209.911 kb on + strand, within HMPREF1078_RS04805 at 210.003 kb on - strand, within HMPREF1078_RS04805 at 210.108 kb on + strand, within HMPREF1078_RS04805 at 210.108 kb on + strand, within HMPREF1078_RS04805 at 210.109 kb on - strand, within HMPREF1078_RS04805 at 210.110 kb on + strand, within HMPREF1078_RS04805 at 210.153 kb on + strand, within HMPREF1078_RS04805 at 210.220 kb on + strand, within HMPREF1078_RS04805 at 210.260 kb on - strand, within HMPREF1078_RS04805 at 210.261 kb on + strand, within HMPREF1078_RS04805 at 210.269 kb on + strand, within HMPREF1078_RS04805 at 210.336 kb on + strand, within HMPREF1078_RS04805 at 210.410 kb on - strand, within HMPREF1078_RS04805 at 210.434 kb on + strand, within HMPREF1078_RS04805 at 210.485 kb on - strand, within HMPREF1078_RS04805 at 210.489 kb on + strand, within HMPREF1078_RS04805 at 210.506 kb on - strand, within HMPREF1078_RS04805 at 210.565 kb on - strand, within HMPREF1078_RS04805 at 210.595 kb on + strand, within HMPREF1078_RS04805 at 210.725 kb on - strand, within HMPREF1078_RS04805 at 210.725 kb on - strand, within HMPREF1078_RS04805 at 210.857 kb on - strand, within HMPREF1078_RS04805 at 210.859 kb on - strand, within HMPREF1078_RS04805 at 210.859 kb on - strand, within HMPREF1078_RS04805 at 210.859 kb on - strand, within HMPREF1078_RS04805 at 210.859 kb on - strand, within HMPREF1078_RS04805 at 211.024 kb on - strand, within HMPREF1078_RS04805 at 211.288 kb on + strand, within HMPREF1078_RS04805 at 211.296 kb on + strand, within HMPREF1078_RS04805 at 211.301 kb on - strand, within HMPREF1078_RS04805 at 211.410 kb on - strand, within HMPREF1078_RS04805 at 211.410 kb on - strand, within HMPREF1078_RS04805 at 211.442 kb on - strand, within HMPREF1078_RS04805 at 211.443 kb on + strand, within HMPREF1078_RS04805 at 211.443 kb on + strand, within HMPREF1078_RS04805 at 211.444 kb on - strand, within HMPREF1078_RS04805 at 211.444 kb on - strand, within HMPREF1078_RS04805 at 211.480 kb on + strand, within HMPREF1078_RS04805 at 211.481 kb on - strand, within HMPREF1078_RS04805 at 211.520 kb on - strand, within HMPREF1078_RS04805 at 211.861 kb on + strand at 211.861 kb on + strand at 211.862 kb on - strand at 212.015 kb on - strand at 212.015 kb on - strand at 212.015 kb on - strand at 212.015 kb on - strand at 212.015 kb on - strand at 212.042 kb on - strand at 212.042 kb on - strand at 212.355 kb on - strand at 212.364 kb on - strand at 212.420 kb on + strand at 212.420 kb on + strand at 212.420 kb on + strand at 212.420 kb on + strand at 212.421 kb on - strand at 212.421 kb on - strand at 212.421 kb on - strand at 212.421 kb on - strand at 212.429 kb on - strand at 212.429 kb on - strand at 212.468 kb on + strand at 212.468 kb on + strand at 212.469 kb on - strand at 212.469 kb on - strand at 212.470 kb on + strand at 212.493 kb on - strand at 212.527 kb on + strand at 212.538 kb on + strand at 212.560 kb on - strand at 212.565 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dextrin from potato starch 2.5 mg/mL (C) remove 208,733 - +2.3 208,805 + -0.9 208,814 + -1.9 208,814 + -2.3 208,824 + -0.6 208,825 - +0.1 208,825 - -0.2 208,887 - -0.8 208,894 + -1.1 208,895 - -0.3 208,895 - -0.6 208,895 - +3.2 208,895 - +0.7 208,927 + +0.5 208,928 - +0.2 209,171 + HMPREF1078_RS04805 0.12 -0.1 209,252 + HMPREF1078_RS04805 0.14 +0.3 209,253 - HMPREF1078_RS04805 0.14 +0.2 209,284 + HMPREF1078_RS04805 0.15 -1.6 209,422 + HMPREF1078_RS04805 0.20 -0.3 209,554 + HMPREF1078_RS04805 0.24 -0.2 209,566 - HMPREF1078_RS04805 0.24 +1.3 209,566 - HMPREF1078_RS04805 0.24 -0.2 209,568 - HMPREF1078_RS04805 0.24 +1.3 209,766 - HMPREF1078_RS04805 0.30 -0.6 209,911 + HMPREF1078_RS04805 0.35 -1.5 210,003 - HMPREF1078_RS04805 0.38 -0.5 210,108 + HMPREF1078_RS04805 0.41 -0.5 210,108 + HMPREF1078_RS04805 0.41 +0.8 210,109 - HMPREF1078_RS04805 0.41 +1.8 210,110 + HMPREF1078_RS04805 0.41 -0.9 210,153 + HMPREF1078_RS04805 0.42 -1.3 210,220 + HMPREF1078_RS04805 0.44 -1.5 210,260 - HMPREF1078_RS04805 0.46 +0.0 210,261 + HMPREF1078_RS04805 0.46 -0.3 210,269 + HMPREF1078_RS04805 0.46 +0.3 210,336 + HMPREF1078_RS04805 0.48 +1.3 210,410 - HMPREF1078_RS04805 0.50 +0.3 210,434 + HMPREF1078_RS04805 0.51 +0.5 210,485 - HMPREF1078_RS04805 0.53 -1.7 210,489 + HMPREF1078_RS04805 0.53 -0.5 210,506 - HMPREF1078_RS04805 0.53 -1.6 210,565 - HMPREF1078_RS04805 0.55 +0.8 210,595 + HMPREF1078_RS04805 0.56 -0.5 210,725 - HMPREF1078_RS04805 0.60 -0.9 210,725 - HMPREF1078_RS04805 0.60 +1.3 210,857 - HMPREF1078_RS04805 0.64 -0.2 210,859 - HMPREF1078_RS04805 0.64 -1.7 210,859 - HMPREF1078_RS04805 0.64 +1.8 210,859 - HMPREF1078_RS04805 0.64 +0.8 210,859 - HMPREF1078_RS04805 0.64 +0.3 211,024 - HMPREF1078_RS04805 0.69 -0.3 211,288 + HMPREF1078_RS04805 0.77 -0.9 211,296 + HMPREF1078_RS04805 0.78 +0.0 211,301 - HMPREF1078_RS04805 0.78 -0.6 211,410 - HMPREF1078_RS04805 0.81 +0.0 211,410 - HMPREF1078_RS04805 0.81 -0.5 211,442 - HMPREF1078_RS04805 0.82 -0.3 211,443 + HMPREF1078_RS04805 0.82 -0.2 211,443 + HMPREF1078_RS04805 0.82 +0.5 211,444 - HMPREF1078_RS04805 0.82 -0.5 211,444 - HMPREF1078_RS04805 0.82 -0.6 211,480 + HMPREF1078_RS04805 0.83 -1.2 211,481 - HMPREF1078_RS04805 0.83 -0.7 211,520 - HMPREF1078_RS04805 0.85 +1.3 211,861 + -0.5 211,861 + -0.2 211,862 - -1.1 212,015 - -0.0 212,015 - -2.3 212,015 - -0.1 212,015 - -0.5 212,015 - -1.8 212,042 - -2.4 212,042 - +1.5 212,355 - +0.2 212,364 - -1.9 212,420 + -1.1 212,420 + +0.5 212,420 + +0.0 212,420 + -0.3 212,421 - +0.3 212,421 - +0.2 212,421 - -0.3 212,421 - -0.6 212,429 - -0.1 212,429 - -0.6 212,468 + +1.0 212,468 + -0.0 212,469 - +0.2 212,469 - -1.3 212,470 + +2.3 212,493 - -1.5 212,527 + -0.6 212,538 + -1.3 212,560 - -1.4 212,565 - +0.1
Or see this region's nucleotide sequence