Experiment: Supernatant; Pseudomonas DC3000 grown in LB; 0.5 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt JOY50_RS23750 and rpoS are separated by 108 nucleotides rpoS and fdxA are separated by 601 nucleotides
JOY50_RS23750: JOY50_RS23750 - peptidoglycan DD-metalloendopeptidase family protein, at 23,767 to 24,624
_RS23750
JOY50_RS23755: rpoS - RNA polymerase sigma factor RpoS, at 24,733 to 25,740
rpoS
JOY50_RS23760: fdxA - ferredoxin FdxA, at 26,342 to 26,665
fdxA
Position (kb) 24
25
26 Strain fitness (log2 ratio) -2
-1
0
1 at 23.759 kb on + strand at 23.832 kb on + strand at 23.940 kb on + strand, within JOY50_RS23750 at 24.664 kb on + strand at 24.681 kb on + strand at 24.932 kb on + strand, within rpoS at 24.947 kb on + strand, within rpoS at 24.976 kb on + strand, within rpoS at 24.995 kb on + strand, within rpoS at 25.121 kb on + strand, within rpoS at 25.121 kb on + strand, within rpoS at 25.121 kb on + strand, within rpoS at 25.126 kb on + strand, within rpoS at 25.223 kb on + strand, within rpoS at 25.233 kb on + strand, within rpoS at 25.277 kb on + strand, within rpoS at 25.374 kb on + strand, within rpoS at 25.385 kb on + strand, within rpoS at 25.440 kb on + strand, within rpoS at 25.440 kb on + strand, within rpoS at 25.580 kb on + strand, within rpoS at 25.580 kb on + strand, within rpoS at 25.651 kb on + strand at 25.677 kb on + strand at 25.711 kb on + strand at 25.740 kb on + strand at 25.767 kb on + strand at 26.164 kb on - strand at 26.247 kb on - strand at 26.265 kb on - strand at 26.356 kb on - strand at 26.727 kb on - strand at 26.727 kb on - strand at 26.727 kb on - strand at 26.727 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Pseudomonas DC3000 grown in LB; 0.5 X remove 23,759 + -0.3 23,832 + -2.0 23,940 + JOY50_RS23750 0.20 +0.8 24,664 + +0.4 24,681 + -0.7 24,932 + rpoS JOY50_RS23755 0.20 +0.0 24,947 + rpoS JOY50_RS23755 0.21 +0.1 24,976 + rpoS JOY50_RS23755 0.24 -0.1 24,995 + rpoS JOY50_RS23755 0.26 +1.4 25,121 + rpoS JOY50_RS23755 0.38 +0.7 25,121 + rpoS JOY50_RS23755 0.38 +0.7 25,121 + rpoS JOY50_RS23755 0.38 -0.3 25,126 + rpoS JOY50_RS23755 0.39 +0.2 25,223 + rpoS JOY50_RS23755 0.49 -0.2 25,233 + rpoS JOY50_RS23755 0.50 +0.9 25,277 + rpoS JOY50_RS23755 0.54 +0.8 25,374 + rpoS JOY50_RS23755 0.64 -0.1 25,385 + rpoS JOY50_RS23755 0.65 -0.2 25,440 + rpoS JOY50_RS23755 0.70 -0.1 25,440 + rpoS JOY50_RS23755 0.70 -0.3 25,580 + rpoS JOY50_RS23755 0.84 +0.3 25,580 + rpoS JOY50_RS23755 0.84 +0.1 25,651 + -0.1 25,677 + -0.4 25,711 + -0.7 25,740 + -1.1 25,767 + -1.0 26,164 - +0.7 26,247 - -0.4 26,265 - +1.0 26,356 - -1.0 26,727 - -0.5 26,727 - -0.6 26,727 - -0.6 26,727 - -0.4
Or see this region's nucleotide sequence