Experiment: D-Raffinose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS04145 and HMPREF1078_RS04150 are separated by 11 nucleotides HMPREF1078_RS04150 and HMPREF1078_RS04155 are separated by 6 nucleotides HMPREF1078_RS04155 and HMPREF1078_RS04160 overlap by 4 nucleotides
HMPREF1078_RS04145: HMPREF1078_RS04145 - peptide chain release factor 3, at 42,677 to 44,257
_RS04145
HMPREF1078_RS04150: HMPREF1078_RS04150 - dTDP-4-dehydrorhamnose reductase, at 44,269 to 45,135
_RS04150
HMPREF1078_RS04155: HMPREF1078_RS04155 - DUF4924 family protein, at 45,142 to 45,690
_RS04155
HMPREF1078_RS04160: HMPREF1078_RS04160 - Mur ligase family protein, at 45,687 to 46,613
_RS04160
Position (kb) 45
46 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 44.188 kb on + strand at 44.209 kb on + strand at 44.210 kb on - strand at 44.212 kb on + strand at 44.212 kb on + strand at 44.261 kb on + strand at 44.266 kb on - strand at 44.290 kb on - strand at 45.144 kb on - strand at 45.387 kb on - strand, within HMPREF1078_RS04155 at 45.387 kb on - strand, within HMPREF1078_RS04155 at 45.387 kb on - strand, within HMPREF1078_RS04155 at 45.466 kb on - strand, within HMPREF1078_RS04155 at 45.466 kb on - strand, within HMPREF1078_RS04155 at 45.466 kb on - strand, within HMPREF1078_RS04155 at 45.509 kb on - strand, within HMPREF1078_RS04155 at 45.542 kb on - strand, within HMPREF1078_RS04155 at 45.544 kb on - strand, within HMPREF1078_RS04155 at 45.604 kb on - strand, within HMPREF1078_RS04155 at 45.614 kb on - strand, within HMPREF1078_RS04155 at 45.614 kb on - strand, within HMPREF1078_RS04155 at 45.614 kb on - strand, within HMPREF1078_RS04155 at 45.614 kb on - strand, within HMPREF1078_RS04155 at 45.614 kb on - strand, within HMPREF1078_RS04155 at 45.639 kb on - strand at 45.639 kb on - strand at 45.639 kb on - strand at 45.648 kb on - strand at 45.648 kb on - strand at 45.702 kb on + strand at 45.703 kb on - strand at 45.707 kb on + strand at 45.708 kb on - strand at 45.708 kb on - strand at 45.708 kb on - strand at 45.809 kb on + strand, within HMPREF1078_RS04160 at 45.845 kb on - strand, within HMPREF1078_RS04160 at 45.884 kb on - strand, within HMPREF1078_RS04160 at 45.992 kb on + strand, within HMPREF1078_RS04160 at 46.064 kb on + strand, within HMPREF1078_RS04160 at 46.120 kb on - strand, within HMPREF1078_RS04160 at 46.120 kb on - strand, within HMPREF1078_RS04160 at 46.120 kb on - strand, within HMPREF1078_RS04160 at 46.160 kb on - strand, within HMPREF1078_RS04160 at 46.350 kb on + strand, within HMPREF1078_RS04160 at 46.350 kb on + strand, within HMPREF1078_RS04160 at 46.351 kb on - strand, within HMPREF1078_RS04160 at 46.371 kb on + strand, within HMPREF1078_RS04160 at 46.371 kb on + strand, within HMPREF1078_RS04160 at 46.371 kb on + strand, within HMPREF1078_RS04160 at 46.372 kb on - strand, within HMPREF1078_RS04160 at 46.407 kb on + strand, within HMPREF1078_RS04160 at 46.411 kb on - strand, within HMPREF1078_RS04160 at 46.422 kb on + strand, within HMPREF1078_RS04160 at 46.423 kb on - strand, within HMPREF1078_RS04160 at 46.424 kb on + strand, within HMPREF1078_RS04160 at 46.425 kb on - strand, within HMPREF1078_RS04160 at 46.430 kb on + strand, within HMPREF1078_RS04160 at 46.430 kb on + strand, within HMPREF1078_RS04160 at 46.435 kb on - strand, within HMPREF1078_RS04160 at 46.435 kb on - strand, within HMPREF1078_RS04160 at 46.435 kb on - strand, within HMPREF1078_RS04160 at 46.435 kb on - strand, within HMPREF1078_RS04160 at 46.470 kb on - strand, within HMPREF1078_RS04160 at 46.503 kb on - strand, within HMPREF1078_RS04160 at 46.524 kb on + strand at 46.531 kb on - strand at 46.564 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Raffinose 10 mg/mL (C) remove 44,188 + -0.0 44,209 + +0.6 44,210 - +1.5 44,212 + +0.5 44,212 + +1.0 44,261 + +0.2 44,266 - -0.1 44,290 - -3.4 45,144 - +0.3 45,387 - HMPREF1078_RS04155 0.45 -2.3 45,387 - HMPREF1078_RS04155 0.45 -1.3 45,387 - HMPREF1078_RS04155 0.45 -2.4 45,466 - HMPREF1078_RS04155 0.59 -1.5 45,466 - HMPREF1078_RS04155 0.59 -0.6 45,466 - HMPREF1078_RS04155 0.59 +0.2 45,509 - HMPREF1078_RS04155 0.67 -2.8 45,542 - HMPREF1078_RS04155 0.73 -1.2 45,544 - HMPREF1078_RS04155 0.73 -1.0 45,604 - HMPREF1078_RS04155 0.84 +0.2 45,614 - HMPREF1078_RS04155 0.86 -1.0 45,614 - HMPREF1078_RS04155 0.86 -0.4 45,614 - HMPREF1078_RS04155 0.86 -1.3 45,614 - HMPREF1078_RS04155 0.86 -0.1 45,614 - HMPREF1078_RS04155 0.86 -1.4 45,639 - -0.4 45,639 - -0.4 45,639 - -0.7 45,648 - -1.8 45,648 - -2.7 45,702 + +0.4 45,703 - +2.9 45,707 + -0.4 45,708 - +1.0 45,708 - +0.7 45,708 - +0.7 45,809 + HMPREF1078_RS04160 0.13 +2.2 45,845 - HMPREF1078_RS04160 0.17 +1.8 45,884 - HMPREF1078_RS04160 0.21 +0.3 45,992 + HMPREF1078_RS04160 0.33 -0.4 46,064 + HMPREF1078_RS04160 0.41 +0.8 46,120 - HMPREF1078_RS04160 0.47 -1.7 46,120 - HMPREF1078_RS04160 0.47 +1.1 46,120 - HMPREF1078_RS04160 0.47 +0.3 46,160 - HMPREF1078_RS04160 0.51 +0.6 46,350 + HMPREF1078_RS04160 0.72 +0.9 46,350 + HMPREF1078_RS04160 0.72 +0.0 46,351 - HMPREF1078_RS04160 0.72 +0.3 46,371 + HMPREF1078_RS04160 0.74 +2.4 46,371 + HMPREF1078_RS04160 0.74 -0.7 46,371 + HMPREF1078_RS04160 0.74 +1.3 46,372 - HMPREF1078_RS04160 0.74 +0.9 46,407 + HMPREF1078_RS04160 0.78 +0.3 46,411 - HMPREF1078_RS04160 0.78 +2.2 46,422 + HMPREF1078_RS04160 0.79 +0.7 46,423 - HMPREF1078_RS04160 0.79 +1.6 46,424 + HMPREF1078_RS04160 0.80 +1.7 46,425 - HMPREF1078_RS04160 0.80 +0.0 46,430 + HMPREF1078_RS04160 0.80 +2.2 46,430 + HMPREF1078_RS04160 0.80 +0.4 46,435 - HMPREF1078_RS04160 0.81 +0.1 46,435 - HMPREF1078_RS04160 0.81 +1.4 46,435 - HMPREF1078_RS04160 0.81 -1.0 46,435 - HMPREF1078_RS04160 0.81 +0.6 46,470 - HMPREF1078_RS04160 0.84 +2.2 46,503 - HMPREF1078_RS04160 0.88 +0.5 46,524 + +1.3 46,531 - -0.4 46,564 - -0.1
Or see this region's nucleotide sequence