Experiment: Supernatant; Pseudomonas DC3000 grown in LB; 0.3 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt JOY50_RS01170 and JOY50_RS01175 are separated by 171 nucleotides JOY50_RS01175 and JOY50_RS01180 are separated by 63 nucleotides JOY50_RS01180 and JOY50_RS01185 are separated by 135 nucleotides
JOY50_RS01170: JOY50_RS01170 - TolC family outer membrane protein, at 258,977 to 260,335
_RS01170
JOY50_RS01175: JOY50_RS01175 - tRNA-uridine aminocarboxypropyltransferase, at 260,507 to 261,106
_RS01175
JOY50_RS01180: JOY50_RS01180 - PAS domain-containing methyl-accepting chemotaxis protein, at 261,170 to 262,735
_RS01180
JOY50_RS01185: JOY50_RS01185 - LysR family transcriptional regulator, at 262,871 to 263,764
_RS01185
Position (kb) 261
262
263 Strain fitness (log2 ratio) -2
-1
0
1
2 at 260.172 kb on - strand, within JOY50_RS01170 at 260.194 kb on + strand, within JOY50_RS01170 at 260.202 kb on - strand at 260.209 kb on + strand at 260.209 kb on + strand at 260.246 kb on + strand at 260.274 kb on + strand at 260.292 kb on + strand at 260.307 kb on + strand at 260.451 kb on - strand at 260.505 kb on + strand at 260.513 kb on - strand at 260.513 kb on - strand at 260.536 kb on + strand at 260.544 kb on - strand at 260.550 kb on + strand at 260.550 kb on + strand at 260.550 kb on + strand at 260.550 kb on + strand at 260.558 kb on - strand at 260.558 kb on - strand at 260.558 kb on - strand at 260.558 kb on - strand at 260.558 kb on - strand at 260.563 kb on - strand at 260.699 kb on + strand, within JOY50_RS01175 at 260.759 kb on - strand, within JOY50_RS01175 at 260.832 kb on - strand, within JOY50_RS01175 at 260.864 kb on + strand, within JOY50_RS01175 at 260.864 kb on + strand, within JOY50_RS01175 at 260.950 kb on + strand, within JOY50_RS01175 at 260.958 kb on - strand, within JOY50_RS01175 at 260.973 kb on + strand, within JOY50_RS01175 at 260.975 kb on + strand, within JOY50_RS01175 at 260.981 kb on - strand, within JOY50_RS01175 at 260.981 kb on - strand, within JOY50_RS01175 at 260.983 kb on - strand, within JOY50_RS01175 at 260.988 kb on - strand, within JOY50_RS01175 at 261.064 kb on + strand at 261.066 kb on - strand at 261.096 kb on + strand at 261.187 kb on + strand at 261.187 kb on + strand at 261.187 kb on + strand at 261.187 kb on + strand at 261.195 kb on - strand at 261.195 kb on - strand at 261.195 kb on - strand at 261.195 kb on - strand at 261.195 kb on - strand at 261.211 kb on - strand at 261.224 kb on + strand at 261.256 kb on + strand at 261.350 kb on - strand, within JOY50_RS01180 at 261.393 kb on - strand, within JOY50_RS01180 at 261.403 kb on - strand, within JOY50_RS01180 at 261.423 kb on - strand, within JOY50_RS01180 at 261.423 kb on - strand, within JOY50_RS01180 at 261.461 kb on - strand, within JOY50_RS01180 at 261.507 kb on - strand, within JOY50_RS01180 at 261.611 kb on + strand, within JOY50_RS01180 at 261.617 kb on - strand, within JOY50_RS01180 at 261.706 kb on + strand, within JOY50_RS01180 at 261.760 kb on + strand, within JOY50_RS01180 at 261.760 kb on - strand, within JOY50_RS01180 at 261.760 kb on - strand, within JOY50_RS01180 at 261.760 kb on - strand, within JOY50_RS01180 at 261.770 kb on + strand, within JOY50_RS01180 at 261.775 kb on - strand, within JOY50_RS01180 at 261.775 kb on - strand, within JOY50_RS01180 at 261.805 kb on + strand, within JOY50_RS01180 at 261.821 kb on + strand, within JOY50_RS01180 at 261.854 kb on - strand, within JOY50_RS01180 at 261.911 kb on - strand, within JOY50_RS01180 at 262.004 kb on + strand, within JOY50_RS01180 at 262.004 kb on + strand, within JOY50_RS01180 at 262.004 kb on + strand, within JOY50_RS01180 at 262.012 kb on - strand, within JOY50_RS01180 at 262.012 kb on - strand, within JOY50_RS01180 at 262.012 kb on - strand, within JOY50_RS01180 at 262.012 kb on - strand, within JOY50_RS01180 at 262.040 kb on - strand, within JOY50_RS01180 at 262.048 kb on + strand, within JOY50_RS01180 at 262.048 kb on + strand, within JOY50_RS01180 at 262.056 kb on - strand, within JOY50_RS01180 at 262.056 kb on - strand, within JOY50_RS01180 at 262.106 kb on + strand, within JOY50_RS01180 at 262.106 kb on + strand, within JOY50_RS01180 at 262.106 kb on + strand, within JOY50_RS01180 at 262.106 kb on + strand, within JOY50_RS01180 at 262.107 kb on + strand, within JOY50_RS01180 at 262.113 kb on + strand, within JOY50_RS01180 at 262.114 kb on - strand, within JOY50_RS01180 at 262.114 kb on - strand, within JOY50_RS01180 at 262.121 kb on - strand, within JOY50_RS01180 at 262.168 kb on - strand, within JOY50_RS01180 at 262.175 kb on - strand, within JOY50_RS01180 at 262.250 kb on + strand, within JOY50_RS01180 at 262.253 kb on - strand, within JOY50_RS01180 at 262.255 kb on + strand, within JOY50_RS01180 at 262.255 kb on + strand, within JOY50_RS01180 at 262.258 kb on - strand, within JOY50_RS01180 at 262.263 kb on - strand, within JOY50_RS01180 at 262.273 kb on - strand, within JOY50_RS01180 at 262.277 kb on + strand, within JOY50_RS01180 at 262.285 kb on - strand, within JOY50_RS01180 at 262.289 kb on + strand, within JOY50_RS01180 at 262.289 kb on + strand, within JOY50_RS01180 at 262.289 kb on + strand, within JOY50_RS01180 at 262.291 kb on - strand, within JOY50_RS01180 at 262.308 kb on + strand, within JOY50_RS01180 at 262.321 kb on - strand, within JOY50_RS01180 at 262.321 kb on - strand, within JOY50_RS01180 at 262.321 kb on - strand, within JOY50_RS01180 at 262.379 kb on + strand, within JOY50_RS01180 at 262.379 kb on + strand, within JOY50_RS01180 at 262.406 kb on - strand, within JOY50_RS01180 at 262.408 kb on - strand, within JOY50_RS01180 at 262.408 kb on - strand, within JOY50_RS01180 at 262.513 kb on + strand, within JOY50_RS01180 at 262.898 kb on - strand at 262.919 kb on - strand at 263.024 kb on - strand, within JOY50_RS01185 at 263.024 kb on - strand, within JOY50_RS01185 at 263.029 kb on - strand, within JOY50_RS01185 at 263.029 kb on - strand, within JOY50_RS01185 at 263.077 kb on - strand, within JOY50_RS01185 at 263.104 kb on - strand, within JOY50_RS01185 at 263.124 kb on - strand, within JOY50_RS01185 at 263.135 kb on - strand, within JOY50_RS01185 at 263.174 kb on - strand, within JOY50_RS01185 at 263.191 kb on + strand, within JOY50_RS01185 at 263.204 kb on - strand, within JOY50_RS01185 at 263.204 kb on - strand, within JOY50_RS01185 at 263.206 kb on + strand, within JOY50_RS01185 at 263.219 kb on - strand, within JOY50_RS01185 at 263.219 kb on - strand, within JOY50_RS01185 at 263.219 kb on - strand, within JOY50_RS01185 at 263.219 kb on - strand, within JOY50_RS01185 at 263.254 kb on - strand, within JOY50_RS01185 at 263.256 kb on - strand, within JOY50_RS01185 at 263.276 kb on - strand, within JOY50_RS01185 at 263.288 kb on - strand, within JOY50_RS01185 at 263.288 kb on - strand, within JOY50_RS01185 at 263.335 kb on - strand, within JOY50_RS01185 at 263.345 kb on - strand, within JOY50_RS01185 at 263.365 kb on - strand, within JOY50_RS01185 at 263.414 kb on - strand, within JOY50_RS01185 at 263.453 kb on - strand, within JOY50_RS01185 at 263.469 kb on - strand, within JOY50_RS01185 at 263.469 kb on - strand, within JOY50_RS01185 at 263.491 kb on - strand, within JOY50_RS01185 at 263.498 kb on - strand, within JOY50_RS01185 at 263.498 kb on - strand, within JOY50_RS01185 at 263.500 kb on - strand, within JOY50_RS01185 at 263.500 kb on - strand, within JOY50_RS01185 at 263.520 kb on - strand, within JOY50_RS01185 at 263.525 kb on - strand, within JOY50_RS01185 at 263.552 kb on - strand, within JOY50_RS01185 at 263.589 kb on - strand, within JOY50_RS01185 at 263.590 kb on - strand, within JOY50_RS01185 at 263.652 kb on - strand, within JOY50_RS01185 at 263.652 kb on - strand, within JOY50_RS01185
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Pseudomonas DC3000 grown in LB; 0.3 X remove 260,172 - JOY50_RS01170 0.88 -1.2 260,194 + JOY50_RS01170 0.90 -0.4 260,202 - +0.5 260,209 + +0.2 260,209 + +0.9 260,246 + -0.4 260,274 + +1.4 260,292 + +0.4 260,307 + +0.8 260,451 - -0.1 260,505 + -0.7 260,513 - -0.2 260,513 - -0.7 260,536 + -0.6 260,544 - -0.4 260,550 + -0.2 260,550 + +0.0 260,550 + +0.4 260,550 + -1.6 260,558 - +0.6 260,558 - -0.3 260,558 - +0.6 260,558 - +0.3 260,558 - -2.3 260,563 - +0.6 260,699 + JOY50_RS01175 0.32 +0.2 260,759 - JOY50_RS01175 0.42 +0.9 260,832 - JOY50_RS01175 0.54 +0.8 260,864 + JOY50_RS01175 0.59 -0.4 260,864 + JOY50_RS01175 0.59 -0.2 260,950 + JOY50_RS01175 0.74 +0.3 260,958 - JOY50_RS01175 0.75 -0.1 260,973 + JOY50_RS01175 0.78 -0.3 260,975 + JOY50_RS01175 0.78 -0.4 260,981 - JOY50_RS01175 0.79 -0.9 260,981 - JOY50_RS01175 0.79 +1.3 260,983 - JOY50_RS01175 0.79 -1.0 260,988 - JOY50_RS01175 0.80 +0.8 261,064 + -0.3 261,066 - +0.3 261,096 + -0.1 261,187 + +0.0 261,187 + +1.2 261,187 + -0.1 261,187 + -1.9 261,195 - +0.5 261,195 - +0.7 261,195 - -0.1 261,195 - -0.7 261,195 - -1.2 261,211 - -1.1 261,224 + -0.6 261,256 + +0.6 261,350 - JOY50_RS01180 0.11 +0.7 261,393 - JOY50_RS01180 0.14 -0.3 261,403 - JOY50_RS01180 0.15 -2.2 261,423 - JOY50_RS01180 0.16 +1.1 261,423 - JOY50_RS01180 0.16 -0.5 261,461 - JOY50_RS01180 0.19 -0.7 261,507 - JOY50_RS01180 0.22 -0.2 261,611 + JOY50_RS01180 0.28 +0.4 261,617 - JOY50_RS01180 0.29 -0.4 261,706 + JOY50_RS01180 0.34 +1.1 261,760 + JOY50_RS01180 0.38 -0.2 261,760 - JOY50_RS01180 0.38 -0.4 261,760 - JOY50_RS01180 0.38 -1.7 261,760 - JOY50_RS01180 0.38 +0.5 261,770 + JOY50_RS01180 0.38 +0.7 261,775 - JOY50_RS01180 0.39 +0.3 261,775 - JOY50_RS01180 0.39 -0.3 261,805 + JOY50_RS01180 0.41 -0.6 261,821 + JOY50_RS01180 0.42 -0.2 261,854 - JOY50_RS01180 0.44 -0.1 261,911 - JOY50_RS01180 0.47 +0.7 262,004 + JOY50_RS01180 0.53 -0.8 262,004 + JOY50_RS01180 0.53 +0.3 262,004 + JOY50_RS01180 0.53 +1.2 262,012 - JOY50_RS01180 0.54 -0.1 262,012 - JOY50_RS01180 0.54 +0.4 262,012 - JOY50_RS01180 0.54 +0.4 262,012 - JOY50_RS01180 0.54 +1.3 262,040 - JOY50_RS01180 0.56 -0.2 262,048 + JOY50_RS01180 0.56 +1.3 262,048 + JOY50_RS01180 0.56 +0.0 262,056 - JOY50_RS01180 0.57 +0.0 262,056 - JOY50_RS01180 0.57 +0.0 262,106 + JOY50_RS01180 0.60 -0.3 262,106 + JOY50_RS01180 0.60 -0.5 262,106 + JOY50_RS01180 0.60 +0.7 262,106 + JOY50_RS01180 0.60 -1.2 262,107 + JOY50_RS01180 0.60 -0.4 262,113 + JOY50_RS01180 0.60 +0.0 262,114 - JOY50_RS01180 0.60 -0.4 262,114 - JOY50_RS01180 0.60 +2.0 262,121 - JOY50_RS01180 0.61 +0.4 262,168 - JOY50_RS01180 0.64 +0.4 262,175 - JOY50_RS01180 0.64 +0.3 262,250 + JOY50_RS01180 0.69 -0.7 262,253 - JOY50_RS01180 0.69 +0.6 262,255 + JOY50_RS01180 0.69 -1.1 262,255 + JOY50_RS01180 0.69 +0.7 262,258 - JOY50_RS01180 0.69 -0.7 262,263 - JOY50_RS01180 0.70 +0.4 262,273 - JOY50_RS01180 0.70 -1.9 262,277 + JOY50_RS01180 0.71 -0.8 262,285 - JOY50_RS01180 0.71 +1.4 262,289 + JOY50_RS01180 0.71 +0.5 262,289 + JOY50_RS01180 0.71 +1.2 262,289 + JOY50_RS01180 0.71 -0.4 262,291 - JOY50_RS01180 0.72 +0.5 262,308 + JOY50_RS01180 0.73 -0.1 262,321 - JOY50_RS01180 0.73 -0.7 262,321 - JOY50_RS01180 0.73 +0.0 262,321 - JOY50_RS01180 0.73 +1.4 262,379 + JOY50_RS01180 0.77 +0.3 262,379 + JOY50_RS01180 0.77 +0.2 262,406 - JOY50_RS01180 0.79 -0.7 262,408 - JOY50_RS01180 0.79 -1.4 262,408 - JOY50_RS01180 0.79 -0.6 262,513 + JOY50_RS01180 0.86 -0.9 262,898 - +0.0 262,919 - +0.6 263,024 - JOY50_RS01185 0.17 -1.5 263,024 - JOY50_RS01185 0.17 +0.4 263,029 - JOY50_RS01185 0.18 -0.8 263,029 - JOY50_RS01185 0.18 -1.7 263,077 - JOY50_RS01185 0.23 -0.6 263,104 - JOY50_RS01185 0.26 -0.2 263,124 - JOY50_RS01185 0.28 +0.0 263,135 - JOY50_RS01185 0.30 +1.4 263,174 - JOY50_RS01185 0.34 +0.0 263,191 + JOY50_RS01185 0.36 +0.0 263,204 - JOY50_RS01185 0.37 +0.9 263,204 - JOY50_RS01185 0.37 +0.6 263,206 + JOY50_RS01185 0.37 +1.5 263,219 - JOY50_RS01185 0.39 -0.7 263,219 - JOY50_RS01185 0.39 +0.6 263,219 - JOY50_RS01185 0.39 +0.1 263,219 - JOY50_RS01185 0.39 +0.6 263,254 - JOY50_RS01185 0.43 +0.6 263,256 - JOY50_RS01185 0.43 +0.4 263,276 - JOY50_RS01185 0.45 -0.6 263,288 - JOY50_RS01185 0.47 +0.1 263,288 - JOY50_RS01185 0.47 -1.0 263,335 - JOY50_RS01185 0.52 -0.0 263,345 - JOY50_RS01185 0.53 -0.7 263,365 - JOY50_RS01185 0.55 -0.1 263,414 - JOY50_RS01185 0.61 -0.2 263,453 - JOY50_RS01185 0.65 +0.3 263,469 - JOY50_RS01185 0.67 +0.1 263,469 - JOY50_RS01185 0.67 -0.4 263,491 - JOY50_RS01185 0.69 +0.2 263,498 - JOY50_RS01185 0.70 -0.3 263,498 - JOY50_RS01185 0.70 -0.8 263,500 - JOY50_RS01185 0.70 -0.7 263,500 - JOY50_RS01185 0.70 +0.1 263,520 - JOY50_RS01185 0.73 -0.4 263,525 - JOY50_RS01185 0.73 +0.1 263,552 - JOY50_RS01185 0.76 -0.4 263,589 - JOY50_RS01185 0.80 -0.1 263,590 - JOY50_RS01185 0.80 +0.7 263,652 - JOY50_RS01185 0.87 +0.1 263,652 - JOY50_RS01185 0.87 +2.1
Or see this region's nucleotide sequence