Experiment: Sucrose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS17055 and HMPREF1078_RS17060 are separated by 325 nucleotides HMPREF1078_RS17060 and HMPREF1078_RS17065 are separated by 69 nucleotides HMPREF1078_RS17065 and HMPREF1078_RS17070 overlap by 4 nucleotides HMPREF1078_RS17070 and HMPREF1078_RS17075 are separated by 184 nucleotides
HMPREF1078_RS17055: HMPREF1078_RS17055 - trigger factor, at 120,531 to 121,886
_RS17055
HMPREF1078_RS17060: HMPREF1078_RS17060 - RNA-binding protein, at 122,212 to 122,454
_RS17060
HMPREF1078_RS17065: HMPREF1078_RS17065 - CPBP family intramembrane metalloprotease, at 122,524 to 123,429
_RS17065
HMPREF1078_RS17070: HMPREF1078_RS17070 - LPS export ABC transporter ATP-binding protein, at 123,426 to 124,178
_RS17070
HMPREF1078_RS17075: HMPREF1078_RS17075 - ABC transporter permease, at 124,363 to 125,109
_RS17075
Position (kb)
122
123
124 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 121.545 kb on + strand, within HMPREF1078_RS17055 at 121.545 kb on + strand, within HMPREF1078_RS17055 at 121.546 kb on - strand, within HMPREF1078_RS17055 at 121.546 kb on - strand, within HMPREF1078_RS17055 at 121.546 kb on - strand, within HMPREF1078_RS17055 at 121.546 kb on - strand, within HMPREF1078_RS17055 at 121.654 kb on + strand, within HMPREF1078_RS17055 at 121.654 kb on + strand, within HMPREF1078_RS17055 at 121.654 kb on + strand, within HMPREF1078_RS17055 at 121.654 kb on + strand, within HMPREF1078_RS17055 at 121.654 kb on + strand, within HMPREF1078_RS17055 at 121.654 kb on + strand, within HMPREF1078_RS17055 at 121.654 kb on + strand, within HMPREF1078_RS17055 at 121.654 kb on + strand, within HMPREF1078_RS17055 at 121.655 kb on - strand, within HMPREF1078_RS17055 at 121.655 kb on - strand, within HMPREF1078_RS17055 at 121.655 kb on - strand, within HMPREF1078_RS17055 at 121.660 kb on + strand, within HMPREF1078_RS17055 at 121.660 kb on + strand, within HMPREF1078_RS17055 at 121.660 kb on + strand, within HMPREF1078_RS17055 at 121.661 kb on - strand, within HMPREF1078_RS17055 at 121.714 kb on + strand, within HMPREF1078_RS17055 at 121.731 kb on - strand, within HMPREF1078_RS17055 at 121.816 kb on + strand at 121.817 kb on - strand at 121.817 kb on - strand at 121.856 kb on + strand at 121.857 kb on - strand at 121.898 kb on + strand at 121.903 kb on - strand at 121.906 kb on + strand at 121.945 kb on + strand at 121.946 kb on - strand at 122.026 kb on - strand at 122.042 kb on - strand at 122.047 kb on - strand at 122.078 kb on + strand at 122.079 kb on - strand at 122.095 kb on - strand at 122.095 kb on - strand at 122.095 kb on - strand at 122.108 kb on - strand at 122.119 kb on + strand at 122.120 kb on - strand at 122.126 kb on + strand at 122.137 kb on + strand at 122.137 kb on + strand at 122.138 kb on - strand at 122.138 kb on - strand at 122.138 kb on - strand at 122.138 kb on - strand at 122.150 kb on + strand at 122.166 kb on - strand at 122.183 kb on - strand at 122.212 kb on - strand at 122.221 kb on + strand at 122.221 kb on + strand at 122.222 kb on - strand at 122.222 kb on - strand at 122.222 kb on - strand at 122.222 kb on - strand at 122.239 kb on + strand, within HMPREF1078_RS17060 at 122.239 kb on + strand, within HMPREF1078_RS17060 at 122.239 kb on + strand, within HMPREF1078_RS17060 at 122.240 kb on - strand, within HMPREF1078_RS17060 at 122.240 kb on - strand, within HMPREF1078_RS17060 at 122.270 kb on + strand, within HMPREF1078_RS17060 at 122.287 kb on - strand, within HMPREF1078_RS17060 at 122.287 kb on - strand, within HMPREF1078_RS17060 at 122.300 kb on + strand, within HMPREF1078_RS17060 at 122.410 kb on + strand, within HMPREF1078_RS17060 at 122.427 kb on - strand, within HMPREF1078_RS17060 at 122.429 kb on + strand, within HMPREF1078_RS17060 at 122.430 kb on - strand at 122.430 kb on - strand at 122.432 kb on + strand at 122.433 kb on - strand at 122.433 kb on - strand at 122.448 kb on + strand at 122.453 kb on - strand at 122.474 kb on + strand at 122.474 kb on + strand at 122.489 kb on + strand at 122.511 kb on + strand at 122.523 kb on + strand at 122.538 kb on + strand at 122.539 kb on - strand at 122.539 kb on - strand at 122.540 kb on + strand at 122.540 kb on + strand at 122.540 kb on + strand at 122.540 kb on + strand at 122.540 kb on + strand at 122.540 kb on + strand at 122.541 kb on - strand at 122.541 kb on - strand at 122.541 kb on - strand at 122.541 kb on - strand at 122.541 kb on - strand at 122.541 kb on - strand at 122.542 kb on + strand at 122.542 kb on + strand at 122.542 kb on + strand at 122.543 kb on - strand at 122.638 kb on + strand, within HMPREF1078_RS17065 at 122.640 kb on + strand, within HMPREF1078_RS17065 at 122.640 kb on + strand, within HMPREF1078_RS17065 at 122.641 kb on - strand, within HMPREF1078_RS17065 at 122.641 kb on - strand, within HMPREF1078_RS17065 at 122.647 kb on + strand, within HMPREF1078_RS17065 at 122.667 kb on + strand, within HMPREF1078_RS17065 at 122.667 kb on + strand, within HMPREF1078_RS17065 at 122.668 kb on - strand, within HMPREF1078_RS17065 at 122.675 kb on - strand, within HMPREF1078_RS17065 at 122.704 kb on - strand, within HMPREF1078_RS17065 at 122.776 kb on + strand, within HMPREF1078_RS17065 at 122.810 kb on + strand, within HMPREF1078_RS17065 at 122.810 kb on + strand, within HMPREF1078_RS17065 at 122.810 kb on + strand, within HMPREF1078_RS17065 at 122.810 kb on + strand, within HMPREF1078_RS17065 at 122.810 kb on + strand, within HMPREF1078_RS17065 at 122.811 kb on - strand, within HMPREF1078_RS17065 at 122.811 kb on - strand, within HMPREF1078_RS17065 at 122.811 kb on - strand, within HMPREF1078_RS17065 at 122.811 kb on - strand, within HMPREF1078_RS17065 at 122.811 kb on - strand, within HMPREF1078_RS17065 at 122.832 kb on + strand, within HMPREF1078_RS17065 at 122.834 kb on + strand, within HMPREF1078_RS17065 at 122.902 kb on - strand, within HMPREF1078_RS17065 at 122.912 kb on + strand, within HMPREF1078_RS17065 at 122.930 kb on + strand, within HMPREF1078_RS17065 at 122.987 kb on + strand, within HMPREF1078_RS17065 at 122.987 kb on + strand, within HMPREF1078_RS17065 at 122.987 kb on + strand, within HMPREF1078_RS17065 at 122.988 kb on - strand, within HMPREF1078_RS17065 at 122.988 kb on - strand, within HMPREF1078_RS17065 at 123.020 kb on + strand, within HMPREF1078_RS17065 at 123.088 kb on + strand, within HMPREF1078_RS17065 at 123.089 kb on - strand, within HMPREF1078_RS17065 at 123.099 kb on + strand, within HMPREF1078_RS17065 at 123.100 kb on - strand, within HMPREF1078_RS17065 at 123.168 kb on + strand, within HMPREF1078_RS17065 at 123.169 kb on - strand, within HMPREF1078_RS17065 at 123.169 kb on - strand, within HMPREF1078_RS17065 at 123.169 kb on - strand, within HMPREF1078_RS17065 at 123.184 kb on + strand, within HMPREF1078_RS17065 at 123.184 kb on + strand, within HMPREF1078_RS17065 at 123.184 kb on + strand, within HMPREF1078_RS17065 at 123.197 kb on + strand, within HMPREF1078_RS17065 at 123.198 kb on - strand, within HMPREF1078_RS17065 at 123.230 kb on + strand, within HMPREF1078_RS17065 at 123.249 kb on + strand, within HMPREF1078_RS17065 at 123.250 kb on - strand, within HMPREF1078_RS17065 at 123.287 kb on + strand, within HMPREF1078_RS17065 at 123.287 kb on + strand, within HMPREF1078_RS17065 at 123.403 kb on - strand at 124.221 kb on - strand at 124.221 kb on - strand at 124.223 kb on - strand at 124.252 kb on + strand at 124.264 kb on + strand at 124.264 kb on + strand at 124.357 kb on - strand at 124.369 kb on - strand at 124.376 kb on + strand at 124.376 kb on + strand at 124.376 kb on + strand at 124.377 kb on - strand at 124.385 kb on + strand at 124.393 kb on + strand at 124.393 kb on + strand at 124.394 kb on - strand at 124.394 kb on - strand at 124.394 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sucrose 10 mg/mL (C) remove 121,545 + HMPREF1078_RS17055 0.75 -1.7 121,545 + HMPREF1078_RS17055 0.75 +0.4 121,546 - HMPREF1078_RS17055 0.75 -1.1 121,546 - HMPREF1078_RS17055 0.75 -0.4 121,546 - HMPREF1078_RS17055 0.75 -0.3 121,546 - HMPREF1078_RS17055 0.75 -0.2 121,654 + HMPREF1078_RS17055 0.83 -0.2 121,654 + HMPREF1078_RS17055 0.83 -0.2 121,654 + HMPREF1078_RS17055 0.83 -0.3 121,654 + HMPREF1078_RS17055 0.83 -0.5 121,654 + HMPREF1078_RS17055 0.83 -0.4 121,654 + HMPREF1078_RS17055 0.83 -0.5 121,654 + HMPREF1078_RS17055 0.83 -0.4 121,654 + HMPREF1078_RS17055 0.83 -1.7 121,655 - HMPREF1078_RS17055 0.83 +0.2 121,655 - HMPREF1078_RS17055 0.83 -1.3 121,655 - HMPREF1078_RS17055 0.83 -1.6 121,660 + HMPREF1078_RS17055 0.83 +0.8 121,660 + HMPREF1078_RS17055 0.83 +0.1 121,660 + HMPREF1078_RS17055 0.83 -0.4 121,661 - HMPREF1078_RS17055 0.83 -0.2 121,714 + HMPREF1078_RS17055 0.87 -0.7 121,731 - HMPREF1078_RS17055 0.88 +0.0 121,816 + +0.2 121,817 - -1.1 121,817 - -0.8 121,856 + -1.6 121,857 - -0.0 121,898 + +0.0 121,903 - -0.6 121,906 + -1.4 121,945 + -0.3 121,946 - -0.2 122,026 - +0.4 122,042 - -0.5 122,047 - -0.5 122,078 + -0.6 122,079 - +1.3 122,095 - -0.9 122,095 - -1.6 122,095 - -0.2 122,108 - -0.2 122,119 + -0.6 122,120 - -0.2 122,126 + +0.4 122,137 + -0.7 122,137 + +0.6 122,138 - +0.3 122,138 - +0.9 122,138 - +0.5 122,138 - +0.8 122,150 + -0.6 122,166 - -0.5 122,183 - -0.3 122,212 - +1.2 122,221 + -0.5 122,221 + -0.8 122,222 - +0.4 122,222 - +0.2 122,222 - -0.1 122,222 - +1.2 122,239 + HMPREF1078_RS17060 0.11 -0.0 122,239 + HMPREF1078_RS17060 0.11 -0.6 122,239 + HMPREF1078_RS17060 0.11 -0.3 122,240 - HMPREF1078_RS17060 0.12 +0.3 122,240 - HMPREF1078_RS17060 0.12 +0.5 122,270 + HMPREF1078_RS17060 0.24 -0.4 122,287 - HMPREF1078_RS17060 0.31 +0.3 122,287 - HMPREF1078_RS17060 0.31 -0.8 122,300 + HMPREF1078_RS17060 0.36 -0.3 122,410 + HMPREF1078_RS17060 0.81 +0.2 122,427 - HMPREF1078_RS17060 0.88 -0.5 122,429 + HMPREF1078_RS17060 0.89 +0.0 122,430 - +0.1 122,430 - -0.7 122,432 + +0.5 122,433 - -0.8 122,433 - +1.2 122,448 + -0.0 122,453 - -0.1 122,474 + +0.4 122,474 + +0.0 122,489 + -0.1 122,511 + +1.4 122,523 + -0.4 122,538 + +0.7 122,539 - -0.7 122,539 - -2.1 122,540 + -0.5 122,540 + -1.6 122,540 + -1.1 122,540 + -0.9 122,540 + +1.0 122,540 + -1.8 122,541 - -1.4 122,541 - +0.7 122,541 - -0.5 122,541 - -0.4 122,541 - -1.0 122,541 - -0.9 122,542 + +0.7 122,542 + -1.9 122,542 + +1.4 122,543 - -0.3 122,638 + HMPREF1078_RS17065 0.13 -1.0 122,640 + HMPREF1078_RS17065 0.13 -0.9 122,640 + HMPREF1078_RS17065 0.13 +0.5 122,641 - HMPREF1078_RS17065 0.13 -1.5 122,641 - HMPREF1078_RS17065 0.13 +0.5 122,647 + HMPREF1078_RS17065 0.14 -2.1 122,667 + HMPREF1078_RS17065 0.16 -2.7 122,667 + HMPREF1078_RS17065 0.16 -1.9 122,668 - HMPREF1078_RS17065 0.16 -1.5 122,675 - HMPREF1078_RS17065 0.17 -1.6 122,704 - HMPREF1078_RS17065 0.20 +1.3 122,776 + HMPREF1078_RS17065 0.28 +0.4 122,810 + HMPREF1078_RS17065 0.32 -1.0 122,810 + HMPREF1078_RS17065 0.32 -1.9 122,810 + HMPREF1078_RS17065 0.32 -0.0 122,810 + HMPREF1078_RS17065 0.32 -1.7 122,810 + HMPREF1078_RS17065 0.32 -1.9 122,811 - HMPREF1078_RS17065 0.32 -0.5 122,811 - HMPREF1078_RS17065 0.32 -1.8 122,811 - HMPREF1078_RS17065 0.32 -1.8 122,811 - HMPREF1078_RS17065 0.32 -1.3 122,811 - HMPREF1078_RS17065 0.32 +1.9 122,832 + HMPREF1078_RS17065 0.34 -1.3 122,834 + HMPREF1078_RS17065 0.34 +1.0 122,902 - HMPREF1078_RS17065 0.42 +0.2 122,912 + HMPREF1078_RS17065 0.43 -0.3 122,930 + HMPREF1078_RS17065 0.45 -0.7 122,987 + HMPREF1078_RS17065 0.51 +0.5 122,987 + HMPREF1078_RS17065 0.51 +1.7 122,987 + HMPREF1078_RS17065 0.51 -1.8 122,988 - HMPREF1078_RS17065 0.51 -0.7 122,988 - HMPREF1078_RS17065 0.51 +0.3 123,020 + HMPREF1078_RS17065 0.55 -0.6 123,088 + HMPREF1078_RS17065 0.62 -1.7 123,089 - HMPREF1078_RS17065 0.62 -0.5 123,099 + HMPREF1078_RS17065 0.63 +0.3 123,100 - HMPREF1078_RS17065 0.64 +0.3 123,168 + HMPREF1078_RS17065 0.71 -2.0 123,169 - HMPREF1078_RS17065 0.71 +0.3 123,169 - HMPREF1078_RS17065 0.71 -2.1 123,169 - HMPREF1078_RS17065 0.71 -1.4 123,184 + HMPREF1078_RS17065 0.73 +0.3 123,184 + HMPREF1078_RS17065 0.73 -0.7 123,184 + HMPREF1078_RS17065 0.73 -0.9 123,197 + HMPREF1078_RS17065 0.74 -1.0 123,198 - HMPREF1078_RS17065 0.74 +0.3 123,230 + HMPREF1078_RS17065 0.78 -0.9 123,249 + HMPREF1078_RS17065 0.80 +1.3 123,250 - HMPREF1078_RS17065 0.80 -0.5 123,287 + HMPREF1078_RS17065 0.84 -1.6 123,287 + HMPREF1078_RS17065 0.84 -1.0 123,403 - -1.0 124,221 - +0.4 124,221 - +0.6 124,223 - -0.8 124,252 + +0.4 124,264 + -0.5 124,264 + +0.2 124,357 - -0.6 124,369 - -0.1 124,376 + -0.3 124,376 + -0.3 124,376 + -1.9 124,377 - -0.5 124,385 + -1.4 124,393 + -1.1 124,393 + -2.0 124,394 - +0.1 124,394 - +0.3 124,394 - -0.2
Or see this region's nucleotide sequence