Experiment: Sucrose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS14610 and HMPREF1078_RS14615 are separated by 76 nucleotides HMPREF1078_RS14615 and HMPREF1078_RS14620 are separated by 135 nucleotides HMPREF1078_RS14620 and HMPREF1078_RS14625 overlap by 4 nucleotides HMPREF1078_RS14625 and HMPREF1078_RS14630 are separated by 15 nucleotides HMPREF1078_RS14630 and HMPREF1078_RS14635 are separated by 1 nucleotides
HMPREF1078_RS14610: HMPREF1078_RS14610 - 6,7-dimethyl-8-ribityllumazine synthase, at 417,526 to 418,008
_RS14610
HMPREF1078_RS14615: HMPREF1078_RS14615 - tetratricopeptide repeat protein, at 418,085 to 418,771
_RS14615
HMPREF1078_RS14620: HMPREF1078_RS14620 - DNA replication and repair protein RecF, at 418,907 to 420,010
_RS14620
HMPREF1078_RS14625: HMPREF1078_RS14625 - DUF721 domain-containing protein, at 420,007 to 420,300
_RS14625
HMPREF1078_RS14630: HMPREF1078_RS14630 - YkvA family protein, at 420,316 to 420,711
_RS14630
HMPREF1078_RS14635: HMPREF1078_RS14635 - 5-formyltetrahydrofolate cyclo-ligase, at 420,713 to 421,228
_RS14635
Position (kb)
418
419
420
421 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 418.060 kb on - strand at 418.060 kb on - strand at 418.062 kb on - strand at 418.062 kb on - strand at 418.062 kb on - strand at 418.062 kb on - strand at 418.068 kb on - strand at 418.068 kb on - strand at 418.068 kb on - strand at 418.070 kb on - strand at 418.080 kb on - strand at 418.080 kb on - strand at 418.080 kb on - strand at 418.080 kb on - strand at 418.082 kb on - strand at 418.082 kb on - strand at 418.082 kb on - strand at 418.082 kb on - strand at 418.082 kb on - strand at 418.082 kb on - strand at 418.082 kb on - strand at 418.087 kb on - strand at 418.087 kb on - strand at 418.087 kb on - strand at 418.087 kb on - strand at 418.115 kb on - strand at 418.115 kb on - strand at 418.115 kb on - strand at 418.117 kb on - strand at 418.117 kb on - strand at 418.117 kb on - strand at 418.117 kb on - strand at 418.174 kb on - strand, within HMPREF1078_RS14615 at 418.320 kb on - strand, within HMPREF1078_RS14615 at 418.320 kb on - strand, within HMPREF1078_RS14615 at 418.320 kb on - strand, within HMPREF1078_RS14615 at 418.324 kb on - strand, within HMPREF1078_RS14615 at 418.324 kb on - strand, within HMPREF1078_RS14615 at 418.429 kb on - strand, within HMPREF1078_RS14615 at 418.580 kb on - strand, within HMPREF1078_RS14615 at 418.615 kb on - strand, within HMPREF1078_RS14615 at 418.662 kb on - strand, within HMPREF1078_RS14615 at 418.664 kb on - strand, within HMPREF1078_RS14615 at 418.667 kb on - strand, within HMPREF1078_RS14615 at 418.667 kb on - strand, within HMPREF1078_RS14615 at 418.780 kb on - strand at 418.789 kb on - strand at 418.792 kb on - strand at 418.797 kb on - strand at 418.797 kb on - strand at 418.894 kb on + strand at 418.897 kb on + strand at 418.927 kb on + strand at 418.980 kb on + strand at 419.039 kb on + strand, within HMPREF1078_RS14620 at 419.068 kb on + strand, within HMPREF1078_RS14620 at 419.069 kb on - strand, within HMPREF1078_RS14620 at 419.122 kb on + strand, within HMPREF1078_RS14620 at 419.122 kb on + strand, within HMPREF1078_RS14620 at 419.123 kb on - strand, within HMPREF1078_RS14620 at 419.126 kb on + strand, within HMPREF1078_RS14620 at 419.126 kb on + strand, within HMPREF1078_RS14620 at 419.126 kb on + strand, within HMPREF1078_RS14620 at 419.132 kb on + strand, within HMPREF1078_RS14620 at 419.200 kb on + strand, within HMPREF1078_RS14620 at 419.280 kb on + strand, within HMPREF1078_RS14620 at 419.345 kb on + strand, within HMPREF1078_RS14620 at 419.361 kb on + strand, within HMPREF1078_RS14620 at 419.368 kb on + strand, within HMPREF1078_RS14620 at 419.368 kb on + strand, within HMPREF1078_RS14620 at 419.414 kb on - strand, within HMPREF1078_RS14620 at 419.459 kb on + strand, within HMPREF1078_RS14620 at 419.474 kb on + strand, within HMPREF1078_RS14620 at 419.474 kb on + strand, within HMPREF1078_RS14620 at 419.525 kb on + strand, within HMPREF1078_RS14620 at 419.570 kb on + strand, within HMPREF1078_RS14620 at 419.572 kb on + strand, within HMPREF1078_RS14620 at 419.572 kb on + strand, within HMPREF1078_RS14620 at 419.647 kb on + strand, within HMPREF1078_RS14620 at 419.738 kb on + strand, within HMPREF1078_RS14620 at 419.765 kb on + strand, within HMPREF1078_RS14620 at 419.765 kb on + strand, within HMPREF1078_RS14620 at 419.905 kb on + strand at 420.287 kb on + strand at 420.288 kb on - strand at 420.288 kb on - strand at 420.291 kb on + strand at 420.315 kb on + strand at 420.382 kb on + strand, within HMPREF1078_RS14630 at 420.383 kb on - strand, within HMPREF1078_RS14630 at 420.383 kb on - strand, within HMPREF1078_RS14630 at 420.449 kb on + strand, within HMPREF1078_RS14630 at 420.449 kb on + strand, within HMPREF1078_RS14630 at 420.449 kb on + strand, within HMPREF1078_RS14630 at 420.449 kb on + strand, within HMPREF1078_RS14630 at 420.450 kb on - strand, within HMPREF1078_RS14630 at 420.450 kb on - strand, within HMPREF1078_RS14630 at 420.472 kb on + strand, within HMPREF1078_RS14630 at 420.473 kb on - strand, within HMPREF1078_RS14630 at 420.507 kb on + strand, within HMPREF1078_RS14630 at 420.507 kb on + strand, within HMPREF1078_RS14630 at 420.508 kb on - strand, within HMPREF1078_RS14630 at 420.508 kb on - strand, within HMPREF1078_RS14630 at 420.514 kb on + strand, within HMPREF1078_RS14630 at 420.515 kb on - strand, within HMPREF1078_RS14630 at 420.516 kb on + strand, within HMPREF1078_RS14630 at 420.517 kb on - strand, within HMPREF1078_RS14630 at 420.517 kb on - strand, within HMPREF1078_RS14630 at 420.517 kb on - strand, within HMPREF1078_RS14630 at 420.517 kb on - strand, within HMPREF1078_RS14630 at 420.522 kb on - strand, within HMPREF1078_RS14630 at 420.531 kb on - strand, within HMPREF1078_RS14630 at 420.541 kb on - strand, within HMPREF1078_RS14630 at 420.591 kb on + strand, within HMPREF1078_RS14630 at 420.592 kb on - strand, within HMPREF1078_RS14630 at 420.603 kb on - strand, within HMPREF1078_RS14630 at 420.603 kb on - strand, within HMPREF1078_RS14630 at 420.639 kb on + strand, within HMPREF1078_RS14630 at 420.639 kb on + strand, within HMPREF1078_RS14630 at 420.656 kb on + strand, within HMPREF1078_RS14630 at 420.676 kb on - strand at 420.676 kb on - strand at 420.676 kb on - strand at 420.703 kb on - strand at 420.719 kb on + strand at 420.735 kb on - strand at 420.736 kb on + strand at 420.737 kb on - strand at 420.737 kb on - strand at 420.737 kb on - strand at 420.785 kb on + strand, within HMPREF1078_RS14635 at 420.798 kb on + strand, within HMPREF1078_RS14635 at 420.875 kb on - strand, within HMPREF1078_RS14635 at 421.002 kb on + strand, within HMPREF1078_RS14635 at 421.002 kb on + strand, within HMPREF1078_RS14635 at 421.002 kb on + strand, within HMPREF1078_RS14635 at 421.002 kb on + strand, within HMPREF1078_RS14635 at 421.002 kb on + strand, within HMPREF1078_RS14635 at 421.003 kb on - strand, within HMPREF1078_RS14635
Per-strain Table
Position Strand Gene LocusTag Fraction Sucrose 10 mg/mL (C) remove 418,060 - +0.0 418,060 - +0.6 418,062 - +0.3 418,062 - -0.7 418,062 - +0.3 418,062 - +0.7 418,068 - -0.2 418,068 - +0.2 418,068 - +0.1 418,070 - +0.6 418,080 - -0.1 418,080 - -0.4 418,080 - +0.5 418,080 - -0.8 418,082 - +0.4 418,082 - -0.8 418,082 - -0.5 418,082 - +1.6 418,082 - -0.7 418,082 - -0.6 418,082 - -0.0 418,087 - +0.2 418,087 - +0.2 418,087 - -2.5 418,087 - -0.5 418,115 - +0.5 418,115 - -2.1 418,115 - -2.9 418,117 - -3.1 418,117 - -0.6 418,117 - +0.2 418,117 - -2.1 418,174 - HMPREF1078_RS14615 0.13 -1.3 418,320 - HMPREF1078_RS14615 0.34 -0.1 418,320 - HMPREF1078_RS14615 0.34 -0.4 418,320 - HMPREF1078_RS14615 0.34 -0.6 418,324 - HMPREF1078_RS14615 0.35 -1.5 418,324 - HMPREF1078_RS14615 0.35 +0.2 418,429 - HMPREF1078_RS14615 0.50 -0.1 418,580 - HMPREF1078_RS14615 0.72 -0.8 418,615 - HMPREF1078_RS14615 0.77 -0.4 418,662 - HMPREF1078_RS14615 0.84 -2.1 418,664 - HMPREF1078_RS14615 0.84 -0.6 418,667 - HMPREF1078_RS14615 0.85 -0.6 418,667 - HMPREF1078_RS14615 0.85 -0.8 418,780 - +0.2 418,789 - -3.0 418,792 - +0.2 418,797 - -1.8 418,797 - -2.8 418,894 + +0.6 418,897 + +0.0 418,927 + -1.3 418,980 + -0.9 419,039 + HMPREF1078_RS14620 0.12 +0.7 419,068 + HMPREF1078_RS14620 0.15 +0.0 419,069 - HMPREF1078_RS14620 0.15 -1.5 419,122 + HMPREF1078_RS14620 0.19 -0.6 419,122 + HMPREF1078_RS14620 0.19 -2.6 419,123 - HMPREF1078_RS14620 0.20 -0.6 419,126 + HMPREF1078_RS14620 0.20 -1.8 419,126 + HMPREF1078_RS14620 0.20 -1.1 419,126 + HMPREF1078_RS14620 0.20 -0.7 419,132 + HMPREF1078_RS14620 0.20 -0.9 419,200 + HMPREF1078_RS14620 0.27 -1.5 419,280 + HMPREF1078_RS14620 0.34 -1.4 419,345 + HMPREF1078_RS14620 0.40 -0.1 419,361 + HMPREF1078_RS14620 0.41 -1.1 419,368 + HMPREF1078_RS14620 0.42 -2.1 419,368 + HMPREF1078_RS14620 0.42 -0.4 419,414 - HMPREF1078_RS14620 0.46 +0.2 419,459 + HMPREF1078_RS14620 0.50 +2.2 419,474 + HMPREF1078_RS14620 0.51 -2.4 419,474 + HMPREF1078_RS14620 0.51 -2.4 419,525 + HMPREF1078_RS14620 0.56 -0.6 419,570 + HMPREF1078_RS14620 0.60 -1.6 419,572 + HMPREF1078_RS14620 0.60 -2.8 419,572 + HMPREF1078_RS14620 0.60 -0.8 419,647 + HMPREF1078_RS14620 0.67 -1.3 419,738 + HMPREF1078_RS14620 0.75 -2.3 419,765 + HMPREF1078_RS14620 0.78 -1.7 419,765 + HMPREF1078_RS14620 0.78 -0.6 419,905 + -0.1 420,287 + +0.5 420,288 - +0.3 420,288 - -1.1 420,291 + +0.8 420,315 + -1.4 420,382 + HMPREF1078_RS14630 0.17 -1.0 420,383 - HMPREF1078_RS14630 0.17 +0.5 420,383 - HMPREF1078_RS14630 0.17 -0.4 420,449 + HMPREF1078_RS14630 0.34 +0.1 420,449 + HMPREF1078_RS14630 0.34 +0.0 420,449 + HMPREF1078_RS14630 0.34 -0.6 420,449 + HMPREF1078_RS14630 0.34 +0.5 420,450 - HMPREF1078_RS14630 0.34 +0.5 420,450 - HMPREF1078_RS14630 0.34 -1.3 420,472 + HMPREF1078_RS14630 0.39 -4.3 420,473 - HMPREF1078_RS14630 0.40 -0.1 420,507 + HMPREF1078_RS14630 0.48 -0.4 420,507 + HMPREF1078_RS14630 0.48 +0.8 420,508 - HMPREF1078_RS14630 0.48 -0.7 420,508 - HMPREF1078_RS14630 0.48 -1.1 420,514 + HMPREF1078_RS14630 0.50 -0.0 420,515 - HMPREF1078_RS14630 0.50 -0.4 420,516 + HMPREF1078_RS14630 0.51 +0.1 420,517 - HMPREF1078_RS14630 0.51 +0.2 420,517 - HMPREF1078_RS14630 0.51 +0.3 420,517 - HMPREF1078_RS14630 0.51 +0.2 420,517 - HMPREF1078_RS14630 0.51 +0.4 420,522 - HMPREF1078_RS14630 0.52 -1.4 420,531 - HMPREF1078_RS14630 0.54 +0.2 420,541 - HMPREF1078_RS14630 0.57 -0.3 420,591 + HMPREF1078_RS14630 0.69 -0.7 420,592 - HMPREF1078_RS14630 0.70 -0.1 420,603 - HMPREF1078_RS14630 0.72 -0.8 420,603 - HMPREF1078_RS14630 0.72 +0.8 420,639 + HMPREF1078_RS14630 0.82 +0.6 420,639 + HMPREF1078_RS14630 0.82 +0.4 420,656 + HMPREF1078_RS14630 0.86 -0.3 420,676 - -0.1 420,676 - -0.2 420,676 - +0.3 420,703 - -0.3 420,719 + -0.7 420,735 - -1.3 420,736 + +0.6 420,737 - +0.1 420,737 - -0.7 420,737 - -0.4 420,785 + HMPREF1078_RS14635 0.14 -0.1 420,798 + HMPREF1078_RS14635 0.16 +0.2 420,875 - HMPREF1078_RS14635 0.31 +0.0 421,002 + HMPREF1078_RS14635 0.56 +0.5 421,002 + HMPREF1078_RS14635 0.56 +0.2 421,002 + HMPREF1078_RS14635 0.56 +0.8 421,002 + HMPREF1078_RS14635 0.56 -2.2 421,002 + HMPREF1078_RS14635 0.56 -2.1 421,003 - HMPREF1078_RS14635 0.56 -0.1
Or see this region's nucleotide sequence