Experiment: NAG 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS13615 and HMPREF1078_RS13620 are separated by 48 nucleotides HMPREF1078_RS13620 and HMPREF1078_RS13625 are separated by 187 nucleotides HMPREF1078_RS13625 and HMPREF1078_RS13630 are separated by 9 nucleotides HMPREF1078_RS13630 and HMPREF1078_RS13635 are separated by 129 nucleotides
HMPREF1078_RS13615: HMPREF1078_RS13615 - RNA polymerase sigma factor, at 172,192 to 172,746
_RS13615
HMPREF1078_RS13620: HMPREF1078_RS13620 - DUF6198 family protein, at 172,795 to 173,466
_RS13620
HMPREF1078_RS13625: HMPREF1078_RS13625 - MarC family protein, at 173,654 to 174,253
_RS13625
HMPREF1078_RS13630: HMPREF1078_RS13630 - M23 family metallopeptidase, at 174,263 to 175,117
_RS13630
HMPREF1078_RS13635: HMPREF1078_RS13635 - mannonate dehydratase, at 175,247 to 176,419
_RS13635
Position (kb) 173
174
175 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 172.656 kb on + strand, within HMPREF1078_RS13615 at 172.656 kb on + strand, within HMPREF1078_RS13615 at 172.656 kb on + strand, within HMPREF1078_RS13615 at 172.661 kb on + strand, within HMPREF1078_RS13615 at 172.661 kb on + strand, within HMPREF1078_RS13615 at 172.662 kb on - strand, within HMPREF1078_RS13615 at 172.748 kb on + strand at 172.768 kb on + strand at 172.845 kb on + strand at 172.868 kb on + strand, within HMPREF1078_RS13620 at 172.868 kb on + strand, within HMPREF1078_RS13620 at 172.869 kb on - strand, within HMPREF1078_RS13620 at 172.888 kb on + strand, within HMPREF1078_RS13620 at 172.889 kb on - strand, within HMPREF1078_RS13620 at 173.001 kb on + strand, within HMPREF1078_RS13620 at 173.007 kb on - strand, within HMPREF1078_RS13620 at 173.007 kb on - strand, within HMPREF1078_RS13620 at 173.022 kb on - strand, within HMPREF1078_RS13620 at 173.085 kb on + strand, within HMPREF1078_RS13620 at 173.086 kb on - strand, within HMPREF1078_RS13620 at 173.086 kb on - strand, within HMPREF1078_RS13620 at 173.110 kb on + strand, within HMPREF1078_RS13620 at 173.110 kb on + strand, within HMPREF1078_RS13620 at 173.111 kb on - strand, within HMPREF1078_RS13620 at 173.129 kb on - strand, within HMPREF1078_RS13620 at 173.130 kb on + strand, within HMPREF1078_RS13620 at 173.130 kb on + strand, within HMPREF1078_RS13620 at 173.131 kb on - strand, within HMPREF1078_RS13620 at 173.131 kb on - strand, within HMPREF1078_RS13620 at 173.131 kb on - strand, within HMPREF1078_RS13620 at 173.132 kb on + strand, within HMPREF1078_RS13620 at 173.133 kb on - strand, within HMPREF1078_RS13620 at 173.133 kb on - strand, within HMPREF1078_RS13620 at 173.133 kb on - strand, within HMPREF1078_RS13620 at 173.166 kb on + strand, within HMPREF1078_RS13620 at 173.176 kb on + strand, within HMPREF1078_RS13620 at 173.177 kb on - strand, within HMPREF1078_RS13620 at 173.221 kb on - strand, within HMPREF1078_RS13620 at 173.224 kb on + strand, within HMPREF1078_RS13620 at 173.224 kb on + strand, within HMPREF1078_RS13620 at 173.225 kb on - strand, within HMPREF1078_RS13620 at 173.272 kb on + strand, within HMPREF1078_RS13620 at 173.272 kb on + strand, within HMPREF1078_RS13620 at 173.273 kb on - strand, within HMPREF1078_RS13620 at 173.275 kb on + strand, within HMPREF1078_RS13620 at 173.276 kb on - strand, within HMPREF1078_RS13620 at 173.284 kb on + strand, within HMPREF1078_RS13620 at 173.284 kb on + strand, within HMPREF1078_RS13620 at 173.285 kb on - strand, within HMPREF1078_RS13620 at 173.285 kb on - strand, within HMPREF1078_RS13620 at 173.290 kb on + strand, within HMPREF1078_RS13620 at 173.302 kb on - strand, within HMPREF1078_RS13620 at 173.302 kb on - strand, within HMPREF1078_RS13620 at 173.335 kb on + strand, within HMPREF1078_RS13620 at 173.336 kb on - strand, within HMPREF1078_RS13620 at 173.386 kb on - strand, within HMPREF1078_RS13620 at 173.432 kb on - strand at 173.460 kb on + strand at 173.460 kb on + strand at 173.466 kb on + strand at 173.466 kb on + strand at 173.467 kb on - strand at 173.467 kb on - strand at 173.467 kb on - strand at 173.467 kb on - strand at 173.484 kb on - strand at 173.501 kb on + strand at 173.509 kb on + strand at 173.509 kb on + strand at 173.509 kb on + strand at 173.509 kb on + strand at 173.510 kb on - strand at 173.510 kb on - strand at 173.516 kb on - strand at 173.516 kb on - strand at 173.555 kb on + strand at 173.590 kb on + strand at 173.590 kb on + strand at 173.593 kb on + strand at 173.640 kb on + strand at 173.640 kb on + strand at 173.653 kb on + strand at 173.720 kb on - strand, within HMPREF1078_RS13625 at 173.722 kb on + strand, within HMPREF1078_RS13625 at 173.723 kb on - strand, within HMPREF1078_RS13625 at 173.740 kb on + strand, within HMPREF1078_RS13625 at 173.743 kb on + strand, within HMPREF1078_RS13625 at 173.795 kb on - strand, within HMPREF1078_RS13625 at 173.845 kb on - strand, within HMPREF1078_RS13625 at 173.932 kb on - strand, within HMPREF1078_RS13625 at 173.994 kb on + strand, within HMPREF1078_RS13625 at 174.024 kb on + strand, within HMPREF1078_RS13625 at 174.025 kb on - strand, within HMPREF1078_RS13625 at 174.025 kb on - strand, within HMPREF1078_RS13625 at 174.025 kb on - strand, within HMPREF1078_RS13625 at 174.027 kb on + strand, within HMPREF1078_RS13625 at 174.027 kb on + strand, within HMPREF1078_RS13625 at 174.028 kb on - strand, within HMPREF1078_RS13625 at 174.028 kb on - strand, within HMPREF1078_RS13625 at 174.028 kb on - strand, within HMPREF1078_RS13625 at 174.044 kb on + strand, within HMPREF1078_RS13625 at 174.044 kb on + strand, within HMPREF1078_RS13625 at 174.045 kb on - strand, within HMPREF1078_RS13625 at 174.045 kb on - strand, within HMPREF1078_RS13625 at 174.045 kb on - strand, within HMPREF1078_RS13625 at 174.049 kb on + strand, within HMPREF1078_RS13625 at 174.050 kb on - strand, within HMPREF1078_RS13625 at 174.050 kb on - strand, within HMPREF1078_RS13625 at 174.066 kb on + strand, within HMPREF1078_RS13625 at 174.072 kb on + strand, within HMPREF1078_RS13625 at 174.075 kb on + strand, within HMPREF1078_RS13625 at 174.083 kb on - strand, within HMPREF1078_RS13625 at 174.152 kb on + strand, within HMPREF1078_RS13625 at 174.152 kb on + strand, within HMPREF1078_RS13625 at 174.153 kb on - strand, within HMPREF1078_RS13625 at 174.153 kb on - strand, within HMPREF1078_RS13625 at 174.153 kb on - strand, within HMPREF1078_RS13625 at 174.154 kb on + strand, within HMPREF1078_RS13625 at 174.155 kb on - strand, within HMPREF1078_RS13625 at 174.178 kb on + strand, within HMPREF1078_RS13625 at 174.208 kb on + strand at 174.214 kb on + strand at 174.217 kb on + strand at 174.231 kb on + strand at 174.232 kb on - strand at 174.288 kb on - strand at 174.321 kb on + strand at 174.321 kb on + strand at 174.322 kb on - strand at 174.322 kb on - strand at 174.415 kb on + strand, within HMPREF1078_RS13630 at 174.476 kb on + strand, within HMPREF1078_RS13630 at 174.477 kb on - strand, within HMPREF1078_RS13630 at 174.502 kb on + strand, within HMPREF1078_RS13630 at 174.502 kb on + strand, within HMPREF1078_RS13630 at 174.552 kb on + strand, within HMPREF1078_RS13630 at 174.553 kb on - strand, within HMPREF1078_RS13630 at 174.553 kb on - strand, within HMPREF1078_RS13630 at 174.553 kb on - strand, within HMPREF1078_RS13630 at 174.583 kb on + strand, within HMPREF1078_RS13630 at 174.584 kb on - strand, within HMPREF1078_RS13630 at 174.584 kb on - strand, within HMPREF1078_RS13630 at 174.584 kb on - strand, within HMPREF1078_RS13630 at 174.591 kb on + strand, within HMPREF1078_RS13630 at 174.591 kb on + strand, within HMPREF1078_RS13630 at 174.591 kb on + strand, within HMPREF1078_RS13630 at 174.592 kb on - strand, within HMPREF1078_RS13630 at 174.592 kb on - strand, within HMPREF1078_RS13630 at 174.612 kb on + strand, within HMPREF1078_RS13630 at 174.613 kb on - strand, within HMPREF1078_RS13630 at 174.620 kb on + strand, within HMPREF1078_RS13630 at 174.629 kb on + strand, within HMPREF1078_RS13630 at 174.640 kb on - strand, within HMPREF1078_RS13630 at 174.646 kb on - strand, within HMPREF1078_RS13630 at 174.646 kb on - strand, within HMPREF1078_RS13630 at 174.648 kb on + strand, within HMPREF1078_RS13630 at 174.655 kb on + strand, within HMPREF1078_RS13630 at 174.656 kb on - strand, within HMPREF1078_RS13630 at 174.656 kb on - strand, within HMPREF1078_RS13630 at 174.659 kb on + strand, within HMPREF1078_RS13630 at 174.687 kb on - strand, within HMPREF1078_RS13630 at 174.694 kb on + strand, within HMPREF1078_RS13630 at 174.694 kb on + strand, within HMPREF1078_RS13630 at 174.695 kb on - strand, within HMPREF1078_RS13630 at 174.695 kb on - strand, within HMPREF1078_RS13630 at 174.720 kb on - strand, within HMPREF1078_RS13630 at 174.740 kb on - strand, within HMPREF1078_RS13630 at 174.750 kb on + strand, within HMPREF1078_RS13630 at 174.762 kb on - strand, within HMPREF1078_RS13630 at 174.789 kb on + strand, within HMPREF1078_RS13630 at 174.790 kb on - strand, within HMPREF1078_RS13630 at 174.790 kb on - strand, within HMPREF1078_RS13630 at 174.896 kb on + strand, within HMPREF1078_RS13630 at 174.897 kb on - strand, within HMPREF1078_RS13630 at 174.903 kb on - strand, within HMPREF1078_RS13630 at 174.903 kb on - strand, within HMPREF1078_RS13630 at 174.904 kb on + strand, within HMPREF1078_RS13630 at 174.930 kb on + strand, within HMPREF1078_RS13630 at 174.930 kb on + strand, within HMPREF1078_RS13630 at 174.931 kb on - strand, within HMPREF1078_RS13630 at 174.985 kb on + strand, within HMPREF1078_RS13630 at 175.073 kb on + strand at 175.073 kb on + strand at 175.074 kb on - strand at 175.074 kb on - strand at 175.077 kb on - strand at 175.079 kb on + strand at 175.080 kb on - strand at 175.082 kb on + strand at 175.101 kb on + strand at 175.138 kb on - strand at 175.173 kb on + strand at 175.249 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG 10 mg/mL (C) remove 172,656 + HMPREF1078_RS13615 0.84 -1.6 172,656 + HMPREF1078_RS13615 0.84 -0.7 172,656 + HMPREF1078_RS13615 0.84 +0.5 172,661 + HMPREF1078_RS13615 0.85 +1.0 172,661 + HMPREF1078_RS13615 0.85 -1.5 172,662 - HMPREF1078_RS13615 0.85 -0.7 172,748 + -0.0 172,768 + +0.3 172,845 + +0.4 172,868 + HMPREF1078_RS13620 0.11 -1.3 172,868 + HMPREF1078_RS13620 0.11 -1.6 172,869 - HMPREF1078_RS13620 0.11 -1.3 172,888 + HMPREF1078_RS13620 0.14 +1.4 172,889 - HMPREF1078_RS13620 0.14 +1.1 173,001 + HMPREF1078_RS13620 0.31 +0.5 173,007 - HMPREF1078_RS13620 0.32 +0.3 173,007 - HMPREF1078_RS13620 0.32 -1.6 173,022 - HMPREF1078_RS13620 0.34 -0.7 173,085 + HMPREF1078_RS13620 0.43 -1.8 173,086 - HMPREF1078_RS13620 0.43 -0.2 173,086 - HMPREF1078_RS13620 0.43 +1.5 173,110 + HMPREF1078_RS13620 0.47 -2.2 173,110 + HMPREF1078_RS13620 0.47 +0.3 173,111 - HMPREF1078_RS13620 0.47 +0.0 173,129 - HMPREF1078_RS13620 0.50 +2.4 173,130 + HMPREF1078_RS13620 0.50 +0.4 173,130 + HMPREF1078_RS13620 0.50 -0.5 173,131 - HMPREF1078_RS13620 0.50 +1.1 173,131 - HMPREF1078_RS13620 0.50 -1.6 173,131 - HMPREF1078_RS13620 0.50 +1.5 173,132 + HMPREF1078_RS13620 0.50 +0.0 173,133 - HMPREF1078_RS13620 0.50 +0.3 173,133 - HMPREF1078_RS13620 0.50 +0.5 173,133 - HMPREF1078_RS13620 0.50 -2.2 173,166 + HMPREF1078_RS13620 0.55 -0.7 173,176 + HMPREF1078_RS13620 0.57 -1.7 173,177 - HMPREF1078_RS13620 0.57 -1.9 173,221 - HMPREF1078_RS13620 0.63 +0.4 173,224 + HMPREF1078_RS13620 0.64 -0.5 173,224 + HMPREF1078_RS13620 0.64 +0.6 173,225 - HMPREF1078_RS13620 0.64 +1.1 173,272 + HMPREF1078_RS13620 0.71 -0.7 173,272 + HMPREF1078_RS13620 0.71 -0.3 173,273 - HMPREF1078_RS13620 0.71 +0.6 173,275 + HMPREF1078_RS13620 0.71 -0.6 173,276 - HMPREF1078_RS13620 0.72 +0.2 173,284 + HMPREF1078_RS13620 0.73 -0.5 173,284 + HMPREF1078_RS13620 0.73 -0.8 173,285 - HMPREF1078_RS13620 0.73 -0.0 173,285 - HMPREF1078_RS13620 0.73 +1.2 173,290 + HMPREF1078_RS13620 0.74 -0.3 173,302 - HMPREF1078_RS13620 0.75 +2.0 173,302 - HMPREF1078_RS13620 0.75 -0.7 173,335 + HMPREF1078_RS13620 0.80 -0.7 173,336 - HMPREF1078_RS13620 0.81 +2.2 173,386 - HMPREF1078_RS13620 0.88 -1.0 173,432 - -1.7 173,460 + +1.5 173,460 + -0.8 173,466 + -1.9 173,466 + +0.2 173,467 - +0.9 173,467 - +0.9 173,467 - +0.3 173,467 - +1.0 173,484 - +1.2 173,501 + +0.0 173,509 + -0.9 173,509 + -0.3 173,509 + -0.2 173,509 + -0.0 173,510 - +0.7 173,510 - +1.2 173,516 - -1.5 173,516 - +0.5 173,555 + -0.4 173,590 + +0.2 173,590 + -2.1 173,593 + +1.2 173,640 + +0.8 173,640 + +0.0 173,653 + -0.5 173,720 - HMPREF1078_RS13625 0.11 +1.0 173,722 + HMPREF1078_RS13625 0.11 -1.9 173,723 - HMPREF1078_RS13625 0.12 +0.7 173,740 + HMPREF1078_RS13625 0.14 -1.5 173,743 + HMPREF1078_RS13625 0.15 +0.6 173,795 - HMPREF1078_RS13625 0.23 -1.3 173,845 - HMPREF1078_RS13625 0.32 -2.8 173,932 - HMPREF1078_RS13625 0.46 -0.2 173,994 + HMPREF1078_RS13625 0.57 -2.3 174,024 + HMPREF1078_RS13625 0.62 -0.3 174,025 - HMPREF1078_RS13625 0.62 -0.0 174,025 - HMPREF1078_RS13625 0.62 +0.3 174,025 - HMPREF1078_RS13625 0.62 -0.8 174,027 + HMPREF1078_RS13625 0.62 -0.5 174,027 + HMPREF1078_RS13625 0.62 -1.7 174,028 - HMPREF1078_RS13625 0.62 +0.7 174,028 - HMPREF1078_RS13625 0.62 -0.8 174,028 - HMPREF1078_RS13625 0.62 -0.3 174,044 + HMPREF1078_RS13625 0.65 -0.6 174,044 + HMPREF1078_RS13625 0.65 +0.4 174,045 - HMPREF1078_RS13625 0.65 +0.4 174,045 - HMPREF1078_RS13625 0.65 -0.6 174,045 - HMPREF1078_RS13625 0.65 -0.2 174,049 + HMPREF1078_RS13625 0.66 -1.9 174,050 - HMPREF1078_RS13625 0.66 -0.2 174,050 - HMPREF1078_RS13625 0.66 -0.4 174,066 + HMPREF1078_RS13625 0.69 +0.2 174,072 + HMPREF1078_RS13625 0.70 -0.7 174,075 + HMPREF1078_RS13625 0.70 +0.9 174,083 - HMPREF1078_RS13625 0.71 -1.2 174,152 + HMPREF1078_RS13625 0.83 -0.2 174,152 + HMPREF1078_RS13625 0.83 +0.6 174,153 - HMPREF1078_RS13625 0.83 -2.3 174,153 - HMPREF1078_RS13625 0.83 -0.3 174,153 - HMPREF1078_RS13625 0.83 -0.6 174,154 + HMPREF1078_RS13625 0.83 +1.0 174,155 - HMPREF1078_RS13625 0.83 -1.7 174,178 + HMPREF1078_RS13625 0.87 -0.6 174,208 + -0.2 174,214 + -0.2 174,217 + +2.2 174,231 + +0.4 174,232 - +0.3 174,288 - -0.2 174,321 + +0.8 174,321 + -1.0 174,322 - +0.5 174,322 - +0.2 174,415 + HMPREF1078_RS13630 0.18 +0.3 174,476 + HMPREF1078_RS13630 0.25 +0.5 174,477 - HMPREF1078_RS13630 0.25 -0.9 174,502 + HMPREF1078_RS13630 0.28 -0.0 174,502 + HMPREF1078_RS13630 0.28 +0.3 174,552 + HMPREF1078_RS13630 0.34 -1.0 174,553 - HMPREF1078_RS13630 0.34 -1.8 174,553 - HMPREF1078_RS13630 0.34 -0.4 174,553 - HMPREF1078_RS13630 0.34 -0.1 174,583 + HMPREF1078_RS13630 0.37 -0.6 174,584 - HMPREF1078_RS13630 0.38 -1.4 174,584 - HMPREF1078_RS13630 0.38 +0.4 174,584 - HMPREF1078_RS13630 0.38 -3.7 174,591 + HMPREF1078_RS13630 0.38 +0.4 174,591 + HMPREF1078_RS13630 0.38 -0.3 174,591 + HMPREF1078_RS13630 0.38 +0.2 174,592 - HMPREF1078_RS13630 0.38 -0.0 174,592 - HMPREF1078_RS13630 0.38 -0.3 174,612 + HMPREF1078_RS13630 0.41 -2.1 174,613 - HMPREF1078_RS13630 0.41 -2.7 174,620 + HMPREF1078_RS13630 0.42 -3.1 174,629 + HMPREF1078_RS13630 0.43 -1.2 174,640 - HMPREF1078_RS13630 0.44 -0.3 174,646 - HMPREF1078_RS13630 0.45 +1.7 174,646 - HMPREF1078_RS13630 0.45 -1.3 174,648 + HMPREF1078_RS13630 0.45 -0.0 174,655 + HMPREF1078_RS13630 0.46 +1.2 174,656 - HMPREF1078_RS13630 0.46 -2.2 174,656 - HMPREF1078_RS13630 0.46 -1.7 174,659 + HMPREF1078_RS13630 0.46 +0.0 174,687 - HMPREF1078_RS13630 0.50 -1.4 174,694 + HMPREF1078_RS13630 0.50 -3.2 174,694 + HMPREF1078_RS13630 0.50 +0.3 174,695 - HMPREF1078_RS13630 0.51 -0.7 174,695 - HMPREF1078_RS13630 0.51 +0.4 174,720 - HMPREF1078_RS13630 0.53 +0.5 174,740 - HMPREF1078_RS13630 0.56 -2.7 174,750 + HMPREF1078_RS13630 0.57 -0.2 174,762 - HMPREF1078_RS13630 0.58 -0.8 174,789 + HMPREF1078_RS13630 0.62 +0.3 174,790 - HMPREF1078_RS13630 0.62 +0.5 174,790 - HMPREF1078_RS13630 0.62 -0.3 174,896 + HMPREF1078_RS13630 0.74 +0.7 174,897 - HMPREF1078_RS13630 0.74 -1.6 174,903 - HMPREF1078_RS13630 0.75 +0.1 174,903 - HMPREF1078_RS13630 0.75 -0.1 174,904 + HMPREF1078_RS13630 0.75 +1.2 174,930 + HMPREF1078_RS13630 0.78 -1.7 174,930 + HMPREF1078_RS13630 0.78 -1.6 174,931 - HMPREF1078_RS13630 0.78 -1.5 174,985 + HMPREF1078_RS13630 0.84 -0.6 175,073 + +0.0 175,073 + -1.3 175,074 - -0.5 175,074 - -0.9 175,077 - -1.4 175,079 + -0.0 175,080 - -0.6 175,082 + -0.1 175,101 + +0.1 175,138 - -0.9 175,173 + -0.6 175,249 - +0.5
Or see this region's nucleotide sequence