Experiment: NAG 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS01725 and HMPREF1078_RS01730 overlap by 4 nucleotides HMPREF1078_RS01730 and HMPREF1078_RS01735 are separated by 49 nucleotides HMPREF1078_RS01735 and HMPREF1078_RS01740 overlap by 4 nucleotides
HMPREF1078_RS01725: HMPREF1078_RS01725 - NAD-dependent DNA ligase LigA, at 432,284 to 434,284
_RS01725
HMPREF1078_RS01730: HMPREF1078_RS01730 - hypothetical protein, at 434,281 to 434,796
_RS01730
HMPREF1078_RS01735: HMPREF1078_RS01735 - 4-hydroxy-tetrahydrodipicolinate synthase, at 434,846 to 435,739
_RS01735
HMPREF1078_RS01740: HMPREF1078_RS01740 - TlpA disulfide reductase family protein, at 435,736 to 436,287
_RS01740
Position (kb) 434
435 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 434.270 kb on - strand at 434.396 kb on - strand, within HMPREF1078_RS01730 at 434.445 kb on + strand, within HMPREF1078_RS01730 at 434.614 kb on - strand, within HMPREF1078_RS01730 at 434.646 kb on + strand, within HMPREF1078_RS01730 at 434.646 kb on + strand, within HMPREF1078_RS01730 at 434.647 kb on - strand, within HMPREF1078_RS01730 at 434.652 kb on - strand, within HMPREF1078_RS01730 at 434.704 kb on + strand, within HMPREF1078_RS01730 at 434.705 kb on - strand, within HMPREF1078_RS01730 at 434.760 kb on + strand at 434.760 kb on + strand at 434.760 kb on + strand at 434.761 kb on - strand at 434.799 kb on + strand at 434.839 kb on + strand at 435.711 kb on + strand at 435.711 kb on + strand at 435.714 kb on + strand at 435.714 kb on + strand at 435.714 kb on + strand at 435.714 kb on + strand at 435.714 kb on + strand at 435.738 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG 10 mg/mL (C) remove 434,270 - -1.8 434,396 - HMPREF1078_RS01730 0.22 -3.9 434,445 + HMPREF1078_RS01730 0.32 -2.7 434,614 - HMPREF1078_RS01730 0.65 -0.5 434,646 + HMPREF1078_RS01730 0.71 -0.4 434,646 + HMPREF1078_RS01730 0.71 -2.9 434,647 - HMPREF1078_RS01730 0.71 -0.3 434,652 - HMPREF1078_RS01730 0.72 -2.1 434,704 + HMPREF1078_RS01730 0.82 +0.7 434,705 - HMPREF1078_RS01730 0.82 -1.1 434,760 + -1.0 434,760 + -1.1 434,760 + -1.4 434,761 - -0.9 434,799 + -0.4 434,839 + +0.0 435,711 + +0.0 435,711 + -1.7 435,714 + +0.4 435,714 + +0.1 435,714 + -0.0 435,714 + +1.3 435,714 + +0.3 435,738 - -1.9
Or see this region's nucleotide sequence