Experiment: NAG 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS01110 and HMPREF1078_RS01115 are separated by 131 nucleotides HMPREF1078_RS01115 and HMPREF1078_RS01120 are separated by 127 nucleotides
HMPREF1078_RS01110: HMPREF1078_RS01110 - DNA topoisomerase 3, at 278,524 to 280,602
_RS01110
HMPREF1078_RS01115: HMPREF1078_RS01115 - DUF4833 domain-containing protein, at 280,734 to 281,282
_RS01115
HMPREF1078_RS01120: HMPREF1078_RS01120 - prephenate dehydrogenase/arogenate dehydrogenase family protein, at 281,410 to 282,183
_RS01120
Position (kb)
280
281
282 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 279.736 kb on - strand, within HMPREF1078_RS01110 at 279.736 kb on - strand, within HMPREF1078_RS01110 at 279.766 kb on - strand, within HMPREF1078_RS01110 at 279.809 kb on + strand, within HMPREF1078_RS01110 at 279.809 kb on + strand, within HMPREF1078_RS01110 at 279.810 kb on - strand, within HMPREF1078_RS01110 at 279.832 kb on + strand, within HMPREF1078_RS01110 at 279.832 kb on + strand, within HMPREF1078_RS01110 at 279.927 kb on + strand, within HMPREF1078_RS01110 at 279.927 kb on + strand, within HMPREF1078_RS01110 at 279.973 kb on - strand, within HMPREF1078_RS01110 at 280.017 kb on + strand, within HMPREF1078_RS01110 at 280.018 kb on - strand, within HMPREF1078_RS01110 at 280.018 kb on - strand, within HMPREF1078_RS01110 at 280.018 kb on - strand, within HMPREF1078_RS01110 at 280.077 kb on - strand, within HMPREF1078_RS01110 at 280.140 kb on - strand, within HMPREF1078_RS01110 at 280.141 kb on + strand, within HMPREF1078_RS01110 at 280.142 kb on - strand, within HMPREF1078_RS01110 at 280.142 kb on - strand, within HMPREF1078_RS01110 at 280.239 kb on + strand, within HMPREF1078_RS01110 at 280.361 kb on + strand, within HMPREF1078_RS01110 at 280.362 kb on - strand, within HMPREF1078_RS01110 at 280.448 kb on + strand at 280.495 kb on + strand at 280.518 kb on + strand at 280.519 kb on - strand at 280.570 kb on + strand at 280.590 kb on + strand at 280.590 kb on + strand at 280.590 kb on + strand at 280.590 kb on + strand at 280.591 kb on - strand at 280.591 kb on - strand at 280.591 kb on - strand at 280.591 kb on - strand at 280.591 kb on - strand at 280.591 kb on - strand at 280.591 kb on - strand at 280.591 kb on - strand at 280.595 kb on - strand at 280.595 kb on - strand at 280.626 kb on + strand at 280.627 kb on - strand at 280.670 kb on - strand at 280.725 kb on - strand at 280.769 kb on + strand at 280.780 kb on - strand at 280.810 kb on - strand, within HMPREF1078_RS01115 at 280.837 kb on + strand, within HMPREF1078_RS01115 at 280.837 kb on + strand, within HMPREF1078_RS01115 at 280.849 kb on - strand, within HMPREF1078_RS01115 at 280.904 kb on + strand, within HMPREF1078_RS01115 at 280.955 kb on + strand, within HMPREF1078_RS01115 at 280.955 kb on + strand, within HMPREF1078_RS01115 at 280.955 kb on + strand, within HMPREF1078_RS01115 at 280.956 kb on - strand, within HMPREF1078_RS01115 at 280.961 kb on - strand, within HMPREF1078_RS01115 at 281.083 kb on - strand, within HMPREF1078_RS01115 at 281.136 kb on - strand, within HMPREF1078_RS01115 at 281.136 kb on - strand, within HMPREF1078_RS01115 at 281.171 kb on - strand, within HMPREF1078_RS01115 at 281.189 kb on + strand, within HMPREF1078_RS01115 at 281.206 kb on + strand, within HMPREF1078_RS01115 at 281.206 kb on + strand, within HMPREF1078_RS01115 at 281.207 kb on - strand, within HMPREF1078_RS01115 at 281.207 kb on - strand, within HMPREF1078_RS01115 at 281.207 kb on - strand, within HMPREF1078_RS01115 at 281.241 kb on + strand at 281.241 kb on + strand at 281.242 kb on - strand at 281.250 kb on + strand at 281.251 kb on - strand at 281.261 kb on + strand at 281.262 kb on - strand at 281.279 kb on - strand at 281.295 kb on + strand at 281.295 kb on + strand at 281.300 kb on + strand at 281.301 kb on - strand at 281.311 kb on + strand at 281.316 kb on - strand at 281.381 kb on + strand at 281.381 kb on + strand at 281.381 kb on + strand at 281.382 kb on - strand at 281.398 kb on + strand at 281.398 kb on + strand at 281.408 kb on + strand at 281.441 kb on + strand at 281.561 kb on + strand, within HMPREF1078_RS01120 at 281.562 kb on - strand, within HMPREF1078_RS01120 at 281.562 kb on - strand, within HMPREF1078_RS01120 at 281.562 kb on - strand, within HMPREF1078_RS01120 at 281.590 kb on + strand, within HMPREF1078_RS01120 at 281.590 kb on + strand, within HMPREF1078_RS01120 at 281.595 kb on - strand, within HMPREF1078_RS01120 at 281.653 kb on + strand, within HMPREF1078_RS01120 at 281.653 kb on + strand, within HMPREF1078_RS01120 at 281.654 kb on - strand, within HMPREF1078_RS01120 at 281.654 kb on - strand, within HMPREF1078_RS01120 at 281.719 kb on - strand, within HMPREF1078_RS01120 at 281.721 kb on - strand, within HMPREF1078_RS01120 at 281.730 kb on + strand, within HMPREF1078_RS01120 at 281.730 kb on + strand, within HMPREF1078_RS01120 at 281.730 kb on + strand, within HMPREF1078_RS01120 at 281.731 kb on - strand, within HMPREF1078_RS01120 at 281.734 kb on + strand, within HMPREF1078_RS01120 at 281.735 kb on - strand, within HMPREF1078_RS01120 at 281.750 kb on + strand, within HMPREF1078_RS01120 at 281.750 kb on + strand, within HMPREF1078_RS01120 at 281.751 kb on - strand, within HMPREF1078_RS01120 at 281.755 kb on - strand, within HMPREF1078_RS01120 at 281.767 kb on + strand, within HMPREF1078_RS01120 at 281.797 kb on - strand, within HMPREF1078_RS01120 at 281.797 kb on - strand, within HMPREF1078_RS01120 at 281.805 kb on + strand, within HMPREF1078_RS01120 at 281.805 kb on + strand, within HMPREF1078_RS01120 at 281.863 kb on - strand, within HMPREF1078_RS01120 at 281.871 kb on - strand, within HMPREF1078_RS01120 at 281.871 kb on - strand, within HMPREF1078_RS01120 at 281.937 kb on + strand, within HMPREF1078_RS01120 at 281.938 kb on - strand, within HMPREF1078_RS01120 at 281.938 kb on - strand, within HMPREF1078_RS01120 at 281.942 kb on + strand, within HMPREF1078_RS01120 at 281.943 kb on - strand, within HMPREF1078_RS01120 at 281.943 kb on - strand, within HMPREF1078_RS01120 at 281.954 kb on + strand, within HMPREF1078_RS01120 at 281.985 kb on + strand, within HMPREF1078_RS01120 at 281.985 kb on + strand, within HMPREF1078_RS01120 at 281.988 kb on - strand, within HMPREF1078_RS01120 at 281.992 kb on + strand, within HMPREF1078_RS01120 at 281.993 kb on - strand, within HMPREF1078_RS01120 at 281.993 kb on - strand, within HMPREF1078_RS01120 at 281.993 kb on - strand, within HMPREF1078_RS01120 at 282.069 kb on - strand, within HMPREF1078_RS01120 at 282.069 kb on - strand, within HMPREF1078_RS01120 at 282.069 kb on - strand, within HMPREF1078_RS01120 at 282.069 kb on - strand, within HMPREF1078_RS01120 at 282.069 kb on - strand, within HMPREF1078_RS01120 at 282.103 kb on + strand, within HMPREF1078_RS01120 at 282.109 kb on + strand at 282.109 kb on + strand at 282.126 kb on + strand at 282.130 kb on + strand at 282.131 kb on - strand at 282.169 kb on + strand at 282.176 kb on - strand at 282.190 kb on + strand at 282.190 kb on + strand at 282.191 kb on - strand at 282.204 kb on - strand at 282.204 kb on - strand at 282.206 kb on + strand at 282.207 kb on - strand at 282.207 kb on - strand at 282.207 kb on - strand at 282.218 kb on - strand at 282.220 kb on + strand at 282.220 kb on - strand at 282.221 kb on - strand at 282.240 kb on + strand at 282.262 kb on + strand at 282.263 kb on - strand at 282.265 kb on + strand at 282.266 kb on - strand at 282.266 kb on - strand at 282.281 kb on + strand at 282.282 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG 10 mg/mL (C) remove 279,736 - HMPREF1078_RS01110 0.58 +0.4 279,736 - HMPREF1078_RS01110 0.58 -0.4 279,766 - HMPREF1078_RS01110 0.60 +0.7 279,809 + HMPREF1078_RS01110 0.62 +0.4 279,809 + HMPREF1078_RS01110 0.62 -0.3 279,810 - HMPREF1078_RS01110 0.62 +0.3 279,832 + HMPREF1078_RS01110 0.63 -2.1 279,832 + HMPREF1078_RS01110 0.63 -0.9 279,927 + HMPREF1078_RS01110 0.67 -1.4 279,927 + HMPREF1078_RS01110 0.67 -0.2 279,973 - HMPREF1078_RS01110 0.70 +0.0 280,017 + HMPREF1078_RS01110 0.72 -1.6 280,018 - HMPREF1078_RS01110 0.72 +0.2 280,018 - HMPREF1078_RS01110 0.72 +0.0 280,018 - HMPREF1078_RS01110 0.72 -1.0 280,077 - HMPREF1078_RS01110 0.75 -2.4 280,140 - HMPREF1078_RS01110 0.78 +0.7 280,141 + HMPREF1078_RS01110 0.78 +0.6 280,142 - HMPREF1078_RS01110 0.78 -0.3 280,142 - HMPREF1078_RS01110 0.78 -0.0 280,239 + HMPREF1078_RS01110 0.82 +0.3 280,361 + HMPREF1078_RS01110 0.88 +0.4 280,362 - HMPREF1078_RS01110 0.88 +1.5 280,448 + -4.5 280,495 + -3.8 280,518 + -0.9 280,519 - -0.9 280,570 + +0.8 280,590 + -0.1 280,590 + +0.3 280,590 + -1.1 280,590 + +0.5 280,591 - +0.4 280,591 - -0.3 280,591 - +0.2 280,591 - +1.0 280,591 - -1.9 280,591 - +1.1 280,591 - +1.7 280,591 - +1.0 280,595 - +0.7 280,595 - -0.5 280,626 + +0.1 280,627 - +0.3 280,670 - -2.9 280,725 - -1.1 280,769 + -1.5 280,780 - -0.2 280,810 - HMPREF1078_RS01115 0.14 -1.2 280,837 + HMPREF1078_RS01115 0.19 +0.6 280,837 + HMPREF1078_RS01115 0.19 +0.5 280,849 - HMPREF1078_RS01115 0.21 -0.2 280,904 + HMPREF1078_RS01115 0.31 +0.2 280,955 + HMPREF1078_RS01115 0.40 -1.8 280,955 + HMPREF1078_RS01115 0.40 -0.5 280,955 + HMPREF1078_RS01115 0.40 -0.5 280,956 - HMPREF1078_RS01115 0.40 -0.6 280,961 - HMPREF1078_RS01115 0.41 -0.5 281,083 - HMPREF1078_RS01115 0.64 -0.7 281,136 - HMPREF1078_RS01115 0.73 -0.4 281,136 - HMPREF1078_RS01115 0.73 -1.5 281,171 - HMPREF1078_RS01115 0.80 -0.2 281,189 + HMPREF1078_RS01115 0.83 -2.1 281,206 + HMPREF1078_RS01115 0.86 -1.5 281,206 + HMPREF1078_RS01115 0.86 -0.3 281,207 - HMPREF1078_RS01115 0.86 -0.5 281,207 - HMPREF1078_RS01115 0.86 -0.8 281,207 - HMPREF1078_RS01115 0.86 +0.1 281,241 + +0.6 281,241 + -0.1 281,242 - +0.0 281,250 + -0.1 281,251 - -0.8 281,261 + -1.0 281,262 - -1.6 281,279 - +0.5 281,295 + +0.3 281,295 + -0.3 281,300 + -0.1 281,301 - +0.5 281,311 + +1.3 281,316 - -1.3 281,381 + -0.1 281,381 + -0.6 281,381 + -0.2 281,382 - +0.1 281,398 + +1.0 281,398 + -0.3 281,408 + +0.5 281,441 + -2.4 281,561 + HMPREF1078_RS01120 0.20 -2.8 281,562 - HMPREF1078_RS01120 0.20 -3.1 281,562 - HMPREF1078_RS01120 0.20 -2.0 281,562 - HMPREF1078_RS01120 0.20 -1.9 281,590 + HMPREF1078_RS01120 0.23 -3.8 281,590 + HMPREF1078_RS01120 0.23 -2.4 281,595 - HMPREF1078_RS01120 0.24 -2.9 281,653 + HMPREF1078_RS01120 0.31 -3.3 281,653 + HMPREF1078_RS01120 0.31 -4.0 281,654 - HMPREF1078_RS01120 0.32 -3.4 281,654 - HMPREF1078_RS01120 0.32 -3.6 281,719 - HMPREF1078_RS01120 0.40 -4.4 281,721 - HMPREF1078_RS01120 0.40 -3.4 281,730 + HMPREF1078_RS01120 0.41 -1.5 281,730 + HMPREF1078_RS01120 0.41 -3.4 281,730 + HMPREF1078_RS01120 0.41 -1.7 281,731 - HMPREF1078_RS01120 0.41 -2.9 281,734 + HMPREF1078_RS01120 0.42 -3.1 281,735 - HMPREF1078_RS01120 0.42 -3.3 281,750 + HMPREF1078_RS01120 0.44 -0.4 281,750 + HMPREF1078_RS01120 0.44 -3.0 281,751 - HMPREF1078_RS01120 0.44 -2.9 281,755 - HMPREF1078_RS01120 0.45 -4.0 281,767 + HMPREF1078_RS01120 0.46 -3.7 281,797 - HMPREF1078_RS01120 0.50 -3.2 281,797 - HMPREF1078_RS01120 0.50 -3.4 281,805 + HMPREF1078_RS01120 0.51 -1.9 281,805 + HMPREF1078_RS01120 0.51 -2.3 281,863 - HMPREF1078_RS01120 0.59 -4.4 281,871 - HMPREF1078_RS01120 0.60 -4.5 281,871 - HMPREF1078_RS01120 0.60 -1.1 281,937 + HMPREF1078_RS01120 0.68 -2.3 281,938 - HMPREF1078_RS01120 0.68 -2.8 281,938 - HMPREF1078_RS01120 0.68 -4.6 281,942 + HMPREF1078_RS01120 0.69 -4.5 281,943 - HMPREF1078_RS01120 0.69 -2.8 281,943 - HMPREF1078_RS01120 0.69 -3.2 281,954 + HMPREF1078_RS01120 0.70 -0.9 281,985 + HMPREF1078_RS01120 0.74 -2.5 281,985 + HMPREF1078_RS01120 0.74 -1.9 281,988 - HMPREF1078_RS01120 0.75 -3.8 281,992 + HMPREF1078_RS01120 0.75 -2.6 281,993 - HMPREF1078_RS01120 0.75 -3.8 281,993 - HMPREF1078_RS01120 0.75 -3.3 281,993 - HMPREF1078_RS01120 0.75 -3.6 282,069 - HMPREF1078_RS01120 0.85 -1.2 282,069 - HMPREF1078_RS01120 0.85 -4.6 282,069 - HMPREF1078_RS01120 0.85 -5.0 282,069 - HMPREF1078_RS01120 0.85 -2.5 282,069 - HMPREF1078_RS01120 0.85 -3.7 282,103 + HMPREF1078_RS01120 0.90 -2.6 282,109 + -2.3 282,109 + -2.1 282,126 + -3.4 282,130 + -3.5 282,131 - -1.6 282,169 + -1.2 282,176 - -2.8 282,190 + -2.2 282,190 + -2.6 282,191 - -1.0 282,204 - -2.6 282,204 - -0.0 282,206 + -2.3 282,207 - +0.6 282,207 - -0.7 282,207 - +0.6 282,218 - +0.8 282,220 + -0.2 282,220 - -2.8 282,221 - +0.4 282,240 + -2.2 282,262 + -3.6 282,263 - -1.6 282,265 + -3.7 282,266 - +0.7 282,266 - -3.3 282,281 + -4.3 282,282 - +0.9
Or see this region's nucleotide sequence