Experiment: D-Mannose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS13560 and HMPREF1078_RS13565 are separated by 155 nucleotides HMPREF1078_RS13565 and HMPREF1078_RS13570 are separated by 18 nucleotides HMPREF1078_RS13570 and HMPREF1078_RS13575 are separated by 6 nucleotides
HMPREF1078_RS13560: HMPREF1078_RS13560 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 159,020 to 159,616
_RS13560
HMPREF1078_RS13565: HMPREF1078_RS13565 - imidazole glycerol phosphate synthase subunit HisF, at 159,772 to 160,527
_RS13565
HMPREF1078_RS13570: HMPREF1078_RS13570 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 160,546 to 161,265
_RS13570
HMPREF1078_RS13575: HMPREF1078_RS13575 - imidazole glycerol phosphate synthase subunit HisH, at 161,272 to 161,862
_RS13575
Position (kb) 160
161
162 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 159.632 kb on + strand at 159.632 kb on + strand at 159.632 kb on + strand at 159.633 kb on - strand at 159.633 kb on - strand at 159.633 kb on - strand at 159.634 kb on + strand at 159.635 kb on - strand at 159.635 kb on - strand at 159.644 kb on - strand at 159.655 kb on - strand at 159.692 kb on + strand at 159.693 kb on - strand at 159.706 kb on + strand at 159.707 kb on - strand at 159.807 kb on - strand at 159.888 kb on - strand, within HMPREF1078_RS13565 at 159.963 kb on + strand, within HMPREF1078_RS13565 at 159.963 kb on + strand, within HMPREF1078_RS13565 at 160.156 kb on + strand, within HMPREF1078_RS13565 at 160.156 kb on + strand, within HMPREF1078_RS13565 at 160.157 kb on - strand, within HMPREF1078_RS13565 at 160.350 kb on + strand, within HMPREF1078_RS13565 at 160.373 kb on - strand, within HMPREF1078_RS13565 at 160.373 kb on - strand, within HMPREF1078_RS13565 at 160.375 kb on - strand, within HMPREF1078_RS13565 at 160.383 kb on - strand, within HMPREF1078_RS13565 at 160.383 kb on - strand, within HMPREF1078_RS13565 at 160.384 kb on + strand, within HMPREF1078_RS13565 at 160.385 kb on - strand, within HMPREF1078_RS13565 at 160.412 kb on + strand, within HMPREF1078_RS13565 at 160.413 kb on - strand, within HMPREF1078_RS13565 at 160.493 kb on - strand at 160.510 kb on + strand at 160.511 kb on - strand at 160.511 kb on - strand at 160.511 kb on - strand at 160.511 kb on - strand at 160.527 kb on + strand at 160.527 kb on + strand at 160.528 kb on - strand at 160.528 kb on - strand at 160.528 kb on - strand at 160.530 kb on - strand at 160.531 kb on + strand at 160.531 kb on + strand at 160.539 kb on - strand at 160.544 kb on + strand at 160.545 kb on - strand at 160.576 kb on + strand at 160.645 kb on - strand, within HMPREF1078_RS13570 at 160.645 kb on - strand, within HMPREF1078_RS13570 at 160.651 kb on + strand, within HMPREF1078_RS13570 at 160.652 kb on - strand, within HMPREF1078_RS13570 at 160.653 kb on + strand, within HMPREF1078_RS13570 at 160.653 kb on + strand, within HMPREF1078_RS13570 at 160.663 kb on - strand, within HMPREF1078_RS13570 at 160.663 kb on - strand, within HMPREF1078_RS13570 at 160.693 kb on + strand, within HMPREF1078_RS13570 at 160.694 kb on - strand, within HMPREF1078_RS13570 at 160.694 kb on - strand, within HMPREF1078_RS13570 at 160.705 kb on + strand, within HMPREF1078_RS13570 at 160.713 kb on + strand, within HMPREF1078_RS13570 at 160.716 kb on - strand, within HMPREF1078_RS13570 at 160.717 kb on + strand, within HMPREF1078_RS13570 at 160.717 kb on + strand, within HMPREF1078_RS13570 at 160.717 kb on + strand, within HMPREF1078_RS13570 at 160.718 kb on - strand, within HMPREF1078_RS13570 at 160.718 kb on - strand, within HMPREF1078_RS13570 at 160.743 kb on + strand, within HMPREF1078_RS13570 at 160.744 kb on - strand, within HMPREF1078_RS13570 at 160.783 kb on - strand, within HMPREF1078_RS13570 at 160.797 kb on - strand, within HMPREF1078_RS13570 at 160.827 kb on - strand, within HMPREF1078_RS13570 at 160.928 kb on - strand, within HMPREF1078_RS13570 at 161.047 kb on - strand, within HMPREF1078_RS13570 at 161.047 kb on - strand, within HMPREF1078_RS13570 at 161.070 kb on + strand, within HMPREF1078_RS13570 at 161.070 kb on + strand, within HMPREF1078_RS13570 at 161.075 kb on + strand, within HMPREF1078_RS13570 at 161.075 kb on + strand, within HMPREF1078_RS13570 at 161.076 kb on - strand, within HMPREF1078_RS13570 at 161.076 kb on - strand, within HMPREF1078_RS13570 at 161.076 kb on - strand, within HMPREF1078_RS13570 at 161.076 kb on - strand, within HMPREF1078_RS13570 at 161.076 kb on - strand, within HMPREF1078_RS13570 at 161.198 kb on + strand at 161.198 kb on + strand at 161.199 kb on - strand at 161.199 kb on - strand at 161.219 kb on - strand at 161.238 kb on + strand at 161.239 kb on - strand at 161.239 kb on - strand at 161.242 kb on + strand at 161.242 kb on + strand at 161.242 kb on + strand at 161.242 kb on + strand at 161.243 kb on - strand at 161.243 kb on - strand at 161.265 kb on + strand at 161.266 kb on - strand at 161.268 kb on + strand at 161.269 kb on - strand at 161.269 kb on - strand at 161.274 kb on - strand at 161.293 kb on + strand at 161.294 kb on - strand at 161.296 kb on + strand at 161.297 kb on - strand at 161.297 kb on - strand at 161.298 kb on + strand at 161.299 kb on - strand at 161.299 kb on - strand at 161.385 kb on + strand, within HMPREF1078_RS13575 at 161.386 kb on - strand, within HMPREF1078_RS13575 at 161.386 kb on - strand, within HMPREF1078_RS13575 at 161.387 kb on + strand, within HMPREF1078_RS13575 at 161.388 kb on - strand, within HMPREF1078_RS13575 at 161.422 kb on + strand, within HMPREF1078_RS13575 at 161.426 kb on + strand, within HMPREF1078_RS13575 at 161.426 kb on + strand, within HMPREF1078_RS13575 at 161.426 kb on + strand, within HMPREF1078_RS13575 at 161.427 kb on - strand, within HMPREF1078_RS13575 at 161.427 kb on - strand, within HMPREF1078_RS13575 at 161.427 kb on - strand, within HMPREF1078_RS13575 at 161.429 kb on + strand, within HMPREF1078_RS13575 at 161.430 kb on - strand, within HMPREF1078_RS13575 at 161.430 kb on - strand, within HMPREF1078_RS13575 at 161.430 kb on - strand, within HMPREF1078_RS13575 at 161.433 kb on + strand, within HMPREF1078_RS13575 at 161.433 kb on + strand, within HMPREF1078_RS13575 at 161.433 kb on + strand, within HMPREF1078_RS13575 at 161.433 kb on + strand, within HMPREF1078_RS13575 at 161.438 kb on - strand, within HMPREF1078_RS13575 at 161.497 kb on - strand, within HMPREF1078_RS13575 at 161.526 kb on + strand, within HMPREF1078_RS13575 at 161.667 kb on + strand, within HMPREF1078_RS13575 at 161.667 kb on + strand, within HMPREF1078_RS13575 at 161.674 kb on - strand, within HMPREF1078_RS13575 at 161.674 kb on - strand, within HMPREF1078_RS13575 at 161.678 kb on - strand, within HMPREF1078_RS13575 at 161.678 kb on - strand, within HMPREF1078_RS13575 at 161.678 kb on - strand, within HMPREF1078_RS13575 at 161.678 kb on - strand, within HMPREF1078_RS13575 at 161.682 kb on + strand, within HMPREF1078_RS13575 at 161.709 kb on + strand, within HMPREF1078_RS13575 at 161.737 kb on + strand, within HMPREF1078_RS13575 at 161.752 kb on - strand, within HMPREF1078_RS13575 at 161.755 kb on + strand, within HMPREF1078_RS13575 at 161.755 kb on + strand, within HMPREF1078_RS13575 at 161.755 kb on + strand, within HMPREF1078_RS13575 at 161.756 kb on - strand, within HMPREF1078_RS13575 at 161.756 kb on - strand, within HMPREF1078_RS13575 at 161.782 kb on - strand, within HMPREF1078_RS13575 at 161.823 kb on - strand at 161.823 kb on - strand at 161.823 kb on - strand at 161.848 kb on - strand at 161.918 kb on - strand at 161.920 kb on - strand at 162.049 kb on + strand at 162.049 kb on + strand at 162.050 kb on - strand at 162.051 kb on - strand at 162.080 kb on - strand at 162.186 kb on + strand at 162.191 kb on + strand at 162.191 kb on + strand at 162.191 kb on + strand at 162.191 kb on + strand at 162.191 kb on + strand at 162.194 kb on + strand at 162.194 kb on + strand at 162.194 kb on + strand at 162.215 kb on + strand at 162.252 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannose 10 mg/mL (C) remove 159,632 + -2.2 159,632 + -2.8 159,632 + -2.7 159,633 - -1.6 159,633 - -1.3 159,633 - +1.5 159,634 + -4.2 159,635 - -0.7 159,635 - -1.6 159,644 - +0.9 159,655 - +0.2 159,692 + -2.3 159,693 - +0.9 159,706 + -5.6 159,707 - -0.9 159,807 - -2.1 159,888 - HMPREF1078_RS13565 0.15 -3.5 159,963 + HMPREF1078_RS13565 0.25 -4.5 159,963 + HMPREF1078_RS13565 0.25 -3.1 160,156 + HMPREF1078_RS13565 0.51 -3.1 160,156 + HMPREF1078_RS13565 0.51 -4.9 160,157 - HMPREF1078_RS13565 0.51 -3.6 160,350 + HMPREF1078_RS13565 0.76 -2.4 160,373 - HMPREF1078_RS13565 0.79 -1.6 160,373 - HMPREF1078_RS13565 0.79 -3.6 160,375 - HMPREF1078_RS13565 0.80 -5.2 160,383 - HMPREF1078_RS13565 0.81 -3.9 160,383 - HMPREF1078_RS13565 0.81 -5.6 160,384 + HMPREF1078_RS13565 0.81 -3.0 160,385 - HMPREF1078_RS13565 0.81 -2.4 160,412 + HMPREF1078_RS13565 0.85 -4.9 160,413 - HMPREF1078_RS13565 0.85 -4.2 160,493 - -3.5 160,510 + -4.4 160,511 - -2.3 160,511 - -4.0 160,511 - -4.6 160,511 - -4.6 160,527 + -2.6 160,527 + -2.8 160,528 - -3.0 160,528 - -4.7 160,528 - -6.6 160,530 - -4.2 160,531 + -5.2 160,531 + -4.4 160,539 - -2.3 160,544 + -4.4 160,545 - -0.3 160,576 + -4.8 160,645 - HMPREF1078_RS13570 0.14 -1.3 160,645 - HMPREF1078_RS13570 0.14 -4.0 160,651 + HMPREF1078_RS13570 0.15 -3.5 160,652 - HMPREF1078_RS13570 0.15 -3.5 160,653 + HMPREF1078_RS13570 0.15 -3.3 160,653 + HMPREF1078_RS13570 0.15 -3.6 160,663 - HMPREF1078_RS13570 0.16 -4.0 160,663 - HMPREF1078_RS13570 0.16 -3.4 160,693 + HMPREF1078_RS13570 0.20 -3.8 160,694 - HMPREF1078_RS13570 0.21 -2.4 160,694 - HMPREF1078_RS13570 0.21 -1.7 160,705 + HMPREF1078_RS13570 0.22 -3.0 160,713 + HMPREF1078_RS13570 0.23 -5.0 160,716 - HMPREF1078_RS13570 0.24 -4.5 160,717 + HMPREF1078_RS13570 0.24 -4.5 160,717 + HMPREF1078_RS13570 0.24 -4.0 160,717 + HMPREF1078_RS13570 0.24 -3.2 160,718 - HMPREF1078_RS13570 0.24 -2.9 160,718 - HMPREF1078_RS13570 0.24 -3.1 160,743 + HMPREF1078_RS13570 0.27 -3.0 160,744 - HMPREF1078_RS13570 0.28 -4.0 160,783 - HMPREF1078_RS13570 0.33 -4.6 160,797 - HMPREF1078_RS13570 0.35 -4.8 160,827 - HMPREF1078_RS13570 0.39 -2.2 160,928 - HMPREF1078_RS13570 0.53 -4.6 161,047 - HMPREF1078_RS13570 0.70 -2.1 161,047 - HMPREF1078_RS13570 0.70 -3.3 161,070 + HMPREF1078_RS13570 0.73 -2.9 161,070 + HMPREF1078_RS13570 0.73 -0.7 161,075 + HMPREF1078_RS13570 0.73 -3.3 161,075 + HMPREF1078_RS13570 0.73 -3.6 161,076 - HMPREF1078_RS13570 0.74 -2.8 161,076 - HMPREF1078_RS13570 0.74 -1.0 161,076 - HMPREF1078_RS13570 0.74 -2.1 161,076 - HMPREF1078_RS13570 0.74 -4.6 161,076 - HMPREF1078_RS13570 0.74 -2.2 161,198 + -3.9 161,198 + -4.2 161,199 - -2.9 161,199 - -2.8 161,219 - -1.2 161,238 + -3.5 161,239 - -0.5 161,239 - -1.6 161,242 + -1.9 161,242 + -4.5 161,242 + -3.6 161,242 + -3.4 161,243 - -3.0 161,243 - -3.2 161,265 + -1.6 161,266 - -3.6 161,268 + -4.0 161,269 - +2.3 161,269 - -3.8 161,274 - -1.3 161,293 + -0.5 161,294 - -3.5 161,296 + -2.0 161,297 - -2.6 161,297 - -2.2 161,298 + -3.8 161,299 - -3.6 161,299 - -4.2 161,385 + HMPREF1078_RS13575 0.19 -2.1 161,386 - HMPREF1078_RS13575 0.19 -5.0 161,386 - HMPREF1078_RS13575 0.19 -3.7 161,387 + HMPREF1078_RS13575 0.19 -5.0 161,388 - HMPREF1078_RS13575 0.20 -4.6 161,422 + HMPREF1078_RS13575 0.25 -4.1 161,426 + HMPREF1078_RS13575 0.26 -2.8 161,426 + HMPREF1078_RS13575 0.26 -3.1 161,426 + HMPREF1078_RS13575 0.26 -0.7 161,427 - HMPREF1078_RS13575 0.26 -1.4 161,427 - HMPREF1078_RS13575 0.26 -5.3 161,427 - HMPREF1078_RS13575 0.26 -2.7 161,429 + HMPREF1078_RS13575 0.27 -1.6 161,430 - HMPREF1078_RS13575 0.27 -2.8 161,430 - HMPREF1078_RS13575 0.27 -2.3 161,430 - HMPREF1078_RS13575 0.27 -3.4 161,433 + HMPREF1078_RS13575 0.27 -1.4 161,433 + HMPREF1078_RS13575 0.27 -2.4 161,433 + HMPREF1078_RS13575 0.27 -3.9 161,433 + HMPREF1078_RS13575 0.27 -4.7 161,438 - HMPREF1078_RS13575 0.28 -4.3 161,497 - HMPREF1078_RS13575 0.38 -4.3 161,526 + HMPREF1078_RS13575 0.43 -3.0 161,667 + HMPREF1078_RS13575 0.67 -1.7 161,667 + HMPREF1078_RS13575 0.67 -2.6 161,674 - HMPREF1078_RS13575 0.68 -4.1 161,674 - HMPREF1078_RS13575 0.68 -3.8 161,678 - HMPREF1078_RS13575 0.69 -4.2 161,678 - HMPREF1078_RS13575 0.69 -4.6 161,678 - HMPREF1078_RS13575 0.69 -2.4 161,678 - HMPREF1078_RS13575 0.69 -3.6 161,682 + HMPREF1078_RS13575 0.69 -2.4 161,709 + HMPREF1078_RS13575 0.74 -3.5 161,737 + HMPREF1078_RS13575 0.79 -2.0 161,752 - HMPREF1078_RS13575 0.81 -3.2 161,755 + HMPREF1078_RS13575 0.82 -3.9 161,755 + HMPREF1078_RS13575 0.82 -5.9 161,755 + HMPREF1078_RS13575 0.82 -3.7 161,756 - HMPREF1078_RS13575 0.82 -4.1 161,756 - HMPREF1078_RS13575 0.82 -5.0 161,782 - HMPREF1078_RS13575 0.86 -2.3 161,823 - -2.6 161,823 - -3.5 161,823 - -2.6 161,848 - -3.5 161,918 - +0.6 161,920 - -0.5 162,049 + -1.5 162,049 + -0.5 162,050 - -0.4 162,051 - -0.0 162,080 - +0.3 162,186 + +0.5 162,191 + -0.8 162,191 + +1.0 162,191 + +0.5 162,191 + -1.2 162,191 + +1.2 162,194 + +1.5 162,194 + -2.0 162,194 + +0.4 162,215 + +0.9 162,252 - +0.6
Or see this region's nucleotide sequence