Experiment: D-Mannose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS11305 and HMPREF1078_RS11310 are separated by 6 nucleotides HMPREF1078_RS11310 and HMPREF1078_RS11315 are separated by 11 nucleotides HMPREF1078_RS11315 and HMPREF1078_RS11320 are separated by 96 nucleotides HMPREF1078_RS11320 and HMPREF1078_RS11325 are separated by 95 nucleotides HMPREF1078_RS11325 and HMPREF1078_RS11330 are separated by 21 nucleotides HMPREF1078_RS11330 and HMPREF1078_RS11335 are separated by 25 nucleotides
HMPREF1078_RS11305: HMPREF1078_RS11305 - orotate phosphoribosyltransferase, at 182,810 to 183,451
_RS11305
HMPREF1078_RS11310: HMPREF1078_RS11310 - polyketide cyclase, at 183,458 to 183,868
_RS11310
HMPREF1078_RS11315: HMPREF1078_RS11315 - argininosuccinate lyase, at 183,880 to 185,217
_RS11315
HMPREF1078_RS11320: HMPREF1078_RS11320 - hypothetical protein, at 185,314 to 185,679
_RS11320
HMPREF1078_RS11325: HMPREF1078_RS11325 - tRNA-Gly, at 185,775 to 185,847
_RS11325
HMPREF1078_RS11330: HMPREF1078_RS11330 - tRNA-Leu, at 185,869 to 185,953
_RS11330
HMPREF1078_RS11335: HMPREF1078_RS11335 - tRNA-Gly, at 185,979 to 186,051
_RS11335
Position (kb)
183
184
185
186 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 183.449 kb on + strand at 183.509 kb on - strand, within HMPREF1078_RS11310 at 183.517 kb on + strand, within HMPREF1078_RS11310 at 183.517 kb on + strand, within HMPREF1078_RS11310 at 183.517 kb on + strand, within HMPREF1078_RS11310 at 183.517 kb on + strand, within HMPREF1078_RS11310 at 183.518 kb on - strand, within HMPREF1078_RS11310 at 183.518 kb on - strand, within HMPREF1078_RS11310 at 183.540 kb on + strand, within HMPREF1078_RS11310 at 183.540 kb on + strand, within HMPREF1078_RS11310 at 183.637 kb on - strand, within HMPREF1078_RS11310 at 183.726 kb on + strand, within HMPREF1078_RS11310 at 183.736 kb on - strand, within HMPREF1078_RS11310 at 183.762 kb on + strand, within HMPREF1078_RS11310 at 183.791 kb on - strand, within HMPREF1078_RS11310 at 183.805 kb on - strand, within HMPREF1078_RS11310 at 183.805 kb on - strand, within HMPREF1078_RS11310 at 183.864 kb on - strand at 183.864 kb on - strand at 183.866 kb on + strand at 183.867 kb on - strand at 183.867 kb on - strand at 183.930 kb on + strand at 183.936 kb on + strand at 183.936 kb on + strand at 183.937 kb on - strand at 183.937 kb on - strand at 183.937 kb on - strand at 183.967 kb on + strand at 183.967 kb on + strand at 183.967 kb on + strand at 183.967 kb on + strand at 183.979 kb on + strand at 183.979 kb on + strand at 183.979 kb on + strand at 183.979 kb on + strand at 184.035 kb on + strand, within HMPREF1078_RS11315 at 184.077 kb on + strand, within HMPREF1078_RS11315 at 184.078 kb on - strand, within HMPREF1078_RS11315 at 184.081 kb on + strand, within HMPREF1078_RS11315 at 184.082 kb on - strand, within HMPREF1078_RS11315 at 184.082 kb on - strand, within HMPREF1078_RS11315 at 184.191 kb on - strand, within HMPREF1078_RS11315 at 184.209 kb on + strand, within HMPREF1078_RS11315 at 184.318 kb on + strand, within HMPREF1078_RS11315 at 184.318 kb on + strand, within HMPREF1078_RS11315 at 184.318 kb on + strand, within HMPREF1078_RS11315 at 184.320 kb on + strand, within HMPREF1078_RS11315 at 184.344 kb on + strand, within HMPREF1078_RS11315 at 184.344 kb on + strand, within HMPREF1078_RS11315 at 184.344 kb on + strand, within HMPREF1078_RS11315 at 184.345 kb on - strand, within HMPREF1078_RS11315 at 184.345 kb on - strand, within HMPREF1078_RS11315 at 184.345 kb on - strand, within HMPREF1078_RS11315 at 184.345 kb on - strand, within HMPREF1078_RS11315 at 184.345 kb on - strand, within HMPREF1078_RS11315 at 184.352 kb on + strand, within HMPREF1078_RS11315 at 184.396 kb on + strand, within HMPREF1078_RS11315 at 184.396 kb on + strand, within HMPREF1078_RS11315 at 184.441 kb on + strand, within HMPREF1078_RS11315 at 184.442 kb on - strand, within HMPREF1078_RS11315 at 184.549 kb on + strand, within HMPREF1078_RS11315 at 184.549 kb on + strand, within HMPREF1078_RS11315 at 184.549 kb on + strand, within HMPREF1078_RS11315 at 184.550 kb on - strand, within HMPREF1078_RS11315 at 184.551 kb on + strand, within HMPREF1078_RS11315 at 184.593 kb on + strand, within HMPREF1078_RS11315 at 184.593 kb on + strand, within HMPREF1078_RS11315 at 184.594 kb on - strand, within HMPREF1078_RS11315 at 184.656 kb on + strand, within HMPREF1078_RS11315 at 184.656 kb on + strand, within HMPREF1078_RS11315 at 184.656 kb on + strand, within HMPREF1078_RS11315 at 184.656 kb on + strand, within HMPREF1078_RS11315 at 184.656 kb on + strand, within HMPREF1078_RS11315 at 184.656 kb on + strand, within HMPREF1078_RS11315 at 184.656 kb on + strand, within HMPREF1078_RS11315 at 184.656 kb on + strand, within HMPREF1078_RS11315 at 184.657 kb on - strand, within HMPREF1078_RS11315 at 184.657 kb on - strand, within HMPREF1078_RS11315 at 184.657 kb on - strand, within HMPREF1078_RS11315 at 184.719 kb on + strand, within HMPREF1078_RS11315 at 184.731 kb on + strand, within HMPREF1078_RS11315 at 184.748 kb on + strand, within HMPREF1078_RS11315 at 184.749 kb on - strand, within HMPREF1078_RS11315 at 184.835 kb on - strand, within HMPREF1078_RS11315 at 184.856 kb on + strand, within HMPREF1078_RS11315 at 184.865 kb on + strand, within HMPREF1078_RS11315 at 184.865 kb on + strand, within HMPREF1078_RS11315 at 184.865 kb on + strand, within HMPREF1078_RS11315 at 184.865 kb on + strand, within HMPREF1078_RS11315 at 184.953 kb on - strand, within HMPREF1078_RS11315 at 184.995 kb on - strand, within HMPREF1078_RS11315 at 185.016 kb on + strand, within HMPREF1078_RS11315 at 185.016 kb on + strand, within HMPREF1078_RS11315 at 185.016 kb on + strand, within HMPREF1078_RS11315 at 185.016 kb on + strand, within HMPREF1078_RS11315 at 185.016 kb on + strand, within HMPREF1078_RS11315 at 185.017 kb on - strand, within HMPREF1078_RS11315 at 185.017 kb on - strand, within HMPREF1078_RS11315 at 185.035 kb on + strand, within HMPREF1078_RS11315 at 185.036 kb on - strand, within HMPREF1078_RS11315 at 185.046 kb on - strand, within HMPREF1078_RS11315 at 185.097 kb on + strand at 185.124 kb on - strand at 185.157 kb on + strand at 185.158 kb on - strand at 185.158 kb on - strand at 185.178 kb on + strand at 185.215 kb on + strand at 185.215 kb on + strand at 185.237 kb on + strand at 185.246 kb on + strand at 185.246 kb on + strand at 185.247 kb on - strand at 185.250 kb on + strand at 185.250 kb on + strand at 185.250 kb on + strand at 185.250 kb on + strand at 185.250 kb on + strand at 185.251 kb on - strand at 185.251 kb on - strand at 185.252 kb on + strand at 185.253 kb on - strand at 185.253 kb on - strand at 185.253 kb on - strand at 185.253 kb on - strand at 185.253 kb on - strand at 185.258 kb on + strand at 185.258 kb on + strand at 185.259 kb on - strand at 185.259 kb on - strand at 185.259 kb on - strand at 185.259 kb on - strand at 185.292 kb on + strand at 185.294 kb on + strand at 185.294 kb on + strand at 185.294 kb on + strand at 185.294 kb on + strand at 185.295 kb on - strand at 185.299 kb on + strand at 185.299 kb on + strand at 185.309 kb on + strand at 185.310 kb on - strand at 185.321 kb on + strand at 185.322 kb on - strand at 185.351 kb on + strand, within HMPREF1078_RS11320 at 185.352 kb on - strand, within HMPREF1078_RS11320 at 185.357 kb on + strand, within HMPREF1078_RS11320 at 185.357 kb on + strand, within HMPREF1078_RS11320 at 185.357 kb on + strand, within HMPREF1078_RS11320 at 185.357 kb on + strand, within HMPREF1078_RS11320 at 185.357 kb on + strand, within HMPREF1078_RS11320 at 185.358 kb on - strand, within HMPREF1078_RS11320 at 185.359 kb on + strand, within HMPREF1078_RS11320 at 185.359 kb on + strand, within HMPREF1078_RS11320 at 185.359 kb on + strand, within HMPREF1078_RS11320 at 185.359 kb on + strand, within HMPREF1078_RS11320 at 185.359 kb on + strand, within HMPREF1078_RS11320 at 185.360 kb on - strand, within HMPREF1078_RS11320 at 185.360 kb on - strand, within HMPREF1078_RS11320 at 185.360 kb on - strand, within HMPREF1078_RS11320 at 185.379 kb on + strand, within HMPREF1078_RS11320 at 185.380 kb on - strand, within HMPREF1078_RS11320 at 185.380 kb on - strand, within HMPREF1078_RS11320 at 185.388 kb on + strand, within HMPREF1078_RS11320 at 185.429 kb on + strand, within HMPREF1078_RS11320 at 185.430 kb on - strand, within HMPREF1078_RS11320 at 185.447 kb on - strand, within HMPREF1078_RS11320 at 185.462 kb on - strand, within HMPREF1078_RS11320 at 185.464 kb on + strand, within HMPREF1078_RS11320 at 185.464 kb on + strand, within HMPREF1078_RS11320 at 185.465 kb on - strand, within HMPREF1078_RS11320 at 185.465 kb on - strand, within HMPREF1078_RS11320 at 185.505 kb on + strand, within HMPREF1078_RS11320 at 185.514 kb on - strand, within HMPREF1078_RS11320 at 185.514 kb on - strand, within HMPREF1078_RS11320 at 185.514 kb on - strand, within HMPREF1078_RS11320 at 185.563 kb on + strand, within HMPREF1078_RS11320 at 185.563 kb on + strand, within HMPREF1078_RS11320 at 185.564 kb on - strand, within HMPREF1078_RS11320 at 185.626 kb on + strand, within HMPREF1078_RS11320 at 185.630 kb on - strand, within HMPREF1078_RS11320 at 185.974 kb on - strand at 185.999 kb on - strand, within HMPREF1078_RS11335 at 186.083 kb on + strand at 186.096 kb on + strand at 186.102 kb on - strand at 186.157 kb on + strand at 186.157 kb on + strand at 186.158 kb on - strand at 186.173 kb on - strand at 186.173 kb on - strand at 186.184 kb on + strand at 186.213 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannose 10 mg/mL (C) remove 183,449 + -1.8 183,509 - HMPREF1078_RS11310 0.12 -1.2 183,517 + HMPREF1078_RS11310 0.14 +0.7 183,517 + HMPREF1078_RS11310 0.14 -0.5 183,517 + HMPREF1078_RS11310 0.14 -0.9 183,517 + HMPREF1078_RS11310 0.14 -0.9 183,518 - HMPREF1078_RS11310 0.15 -0.5 183,518 - HMPREF1078_RS11310 0.15 -1.9 183,540 + HMPREF1078_RS11310 0.20 -1.6 183,540 + HMPREF1078_RS11310 0.20 -3.8 183,637 - HMPREF1078_RS11310 0.44 -2.2 183,726 + HMPREF1078_RS11310 0.65 -1.9 183,736 - HMPREF1078_RS11310 0.68 -2.1 183,762 + HMPREF1078_RS11310 0.74 +0.9 183,791 - HMPREF1078_RS11310 0.81 -1.3 183,805 - HMPREF1078_RS11310 0.84 -1.2 183,805 - HMPREF1078_RS11310 0.84 -2.9 183,864 - -4.3 183,864 - -4.2 183,866 + -1.9 183,867 - -2.8 183,867 - -4.0 183,930 + -2.4 183,936 + -4.6 183,936 + -2.3 183,937 - -3.0 183,937 - -3.8 183,937 - -3.2 183,967 + -3.1 183,967 + -4.7 183,967 + -1.2 183,967 + -2.8 183,979 + -2.4 183,979 + -5.0 183,979 + -4.1 183,979 + -3.1 184,035 + HMPREF1078_RS11315 0.12 -2.4 184,077 + HMPREF1078_RS11315 0.15 -3.4 184,078 - HMPREF1078_RS11315 0.15 -2.6 184,081 + HMPREF1078_RS11315 0.15 -5.5 184,082 - HMPREF1078_RS11315 0.15 -1.4 184,082 - HMPREF1078_RS11315 0.15 -3.3 184,191 - HMPREF1078_RS11315 0.23 -2.4 184,209 + HMPREF1078_RS11315 0.25 -3.0 184,318 + HMPREF1078_RS11315 0.33 -2.3 184,318 + HMPREF1078_RS11315 0.33 -3.2 184,318 + HMPREF1078_RS11315 0.33 -4.8 184,320 + HMPREF1078_RS11315 0.33 -3.8 184,344 + HMPREF1078_RS11315 0.35 -4.0 184,344 + HMPREF1078_RS11315 0.35 -0.5 184,344 + HMPREF1078_RS11315 0.35 -3.2 184,345 - HMPREF1078_RS11315 0.35 -1.8 184,345 - HMPREF1078_RS11315 0.35 -3.9 184,345 - HMPREF1078_RS11315 0.35 -4.4 184,345 - HMPREF1078_RS11315 0.35 -4.3 184,345 - HMPREF1078_RS11315 0.35 -2.8 184,352 + HMPREF1078_RS11315 0.35 -4.2 184,396 + HMPREF1078_RS11315 0.39 -5.8 184,396 + HMPREF1078_RS11315 0.39 -1.5 184,441 + HMPREF1078_RS11315 0.42 -4.4 184,442 - HMPREF1078_RS11315 0.42 -0.5 184,549 + HMPREF1078_RS11315 0.50 -0.9 184,549 + HMPREF1078_RS11315 0.50 -3.3 184,549 + HMPREF1078_RS11315 0.50 -3.3 184,550 - HMPREF1078_RS11315 0.50 -4.4 184,551 + HMPREF1078_RS11315 0.50 -4.5 184,593 + HMPREF1078_RS11315 0.53 -5.6 184,593 + HMPREF1078_RS11315 0.53 -3.2 184,594 - HMPREF1078_RS11315 0.53 -2.7 184,656 + HMPREF1078_RS11315 0.58 -3.8 184,656 + HMPREF1078_RS11315 0.58 -0.3 184,656 + HMPREF1078_RS11315 0.58 -4.5 184,656 + HMPREF1078_RS11315 0.58 -3.2 184,656 + HMPREF1078_RS11315 0.58 -4.2 184,656 + HMPREF1078_RS11315 0.58 -4.9 184,656 + HMPREF1078_RS11315 0.58 -2.4 184,656 + HMPREF1078_RS11315 0.58 -3.1 184,657 - HMPREF1078_RS11315 0.58 -1.6 184,657 - HMPREF1078_RS11315 0.58 -1.4 184,657 - HMPREF1078_RS11315 0.58 -3.3 184,719 + HMPREF1078_RS11315 0.63 -0.0 184,731 + HMPREF1078_RS11315 0.64 -4.3 184,748 + HMPREF1078_RS11315 0.65 -4.1 184,749 - HMPREF1078_RS11315 0.65 -2.4 184,835 - HMPREF1078_RS11315 0.71 -2.6 184,856 + HMPREF1078_RS11315 0.73 -2.3 184,865 + HMPREF1078_RS11315 0.74 -4.4 184,865 + HMPREF1078_RS11315 0.74 -3.8 184,865 + HMPREF1078_RS11315 0.74 -4.1 184,865 + HMPREF1078_RS11315 0.74 -3.7 184,953 - HMPREF1078_RS11315 0.80 -2.4 184,995 - HMPREF1078_RS11315 0.83 -3.2 185,016 + HMPREF1078_RS11315 0.85 -3.2 185,016 + HMPREF1078_RS11315 0.85 -3.9 185,016 + HMPREF1078_RS11315 0.85 -3.6 185,016 + HMPREF1078_RS11315 0.85 -3.7 185,016 + HMPREF1078_RS11315 0.85 -4.6 185,017 - HMPREF1078_RS11315 0.85 -1.6 185,017 - HMPREF1078_RS11315 0.85 -3.2 185,035 + HMPREF1078_RS11315 0.86 -4.6 185,036 - HMPREF1078_RS11315 0.86 -1.7 185,046 - HMPREF1078_RS11315 0.87 -2.1 185,097 + -2.6 185,124 - -3.9 185,157 + -4.6 185,158 - +0.2 185,158 - -2.2 185,178 + -4.4 185,215 + -1.9 185,215 + -0.1 185,237 + -1.2 185,246 + -2.8 185,246 + -1.4 185,247 - -0.5 185,250 + -1.4 185,250 + -2.5 185,250 + -1.6 185,250 + -2.1 185,250 + -3.0 185,251 - -2.6 185,251 - -1.6 185,252 + -2.2 185,253 - -3.4 185,253 - -2.1 185,253 - +0.7 185,253 - -1.0 185,253 - -0.6 185,258 + -3.3 185,258 + -3.2 185,259 - +0.5 185,259 - +1.6 185,259 - -2.6 185,259 - -0.0 185,292 + -2.4 185,294 + -0.2 185,294 + -0.6 185,294 + -2.4 185,294 + -2.0 185,295 - -1.2 185,299 + +0.7 185,299 + -1.4 185,309 + -0.5 185,310 - -2.6 185,321 + -1.9 185,322 - -1.7 185,351 + HMPREF1078_RS11320 0.10 -1.6 185,352 - HMPREF1078_RS11320 0.10 -0.8 185,357 + HMPREF1078_RS11320 0.12 -2.6 185,357 + HMPREF1078_RS11320 0.12 -1.4 185,357 + HMPREF1078_RS11320 0.12 -1.9 185,357 + HMPREF1078_RS11320 0.12 -0.6 185,357 + HMPREF1078_RS11320 0.12 -1.4 185,358 - HMPREF1078_RS11320 0.12 -2.9 185,359 + HMPREF1078_RS11320 0.12 -3.4 185,359 + HMPREF1078_RS11320 0.12 -2.2 185,359 + HMPREF1078_RS11320 0.12 -2.4 185,359 + HMPREF1078_RS11320 0.12 -1.5 185,359 + HMPREF1078_RS11320 0.12 -1.9 185,360 - HMPREF1078_RS11320 0.13 -1.0 185,360 - HMPREF1078_RS11320 0.13 -0.3 185,360 - HMPREF1078_RS11320 0.13 -3.5 185,379 + HMPREF1078_RS11320 0.18 -2.3 185,380 - HMPREF1078_RS11320 0.18 -3.2 185,380 - HMPREF1078_RS11320 0.18 -2.0 185,388 + HMPREF1078_RS11320 0.20 -2.6 185,429 + HMPREF1078_RS11320 0.31 -1.0 185,430 - HMPREF1078_RS11320 0.32 -1.3 185,447 - HMPREF1078_RS11320 0.36 -1.4 185,462 - HMPREF1078_RS11320 0.40 -1.0 185,464 + HMPREF1078_RS11320 0.41 -3.9 185,464 + HMPREF1078_RS11320 0.41 -2.9 185,465 - HMPREF1078_RS11320 0.41 -2.6 185,465 - HMPREF1078_RS11320 0.41 -1.2 185,505 + HMPREF1078_RS11320 0.52 -2.7 185,514 - HMPREF1078_RS11320 0.55 -2.3 185,514 - HMPREF1078_RS11320 0.55 -2.9 185,514 - HMPREF1078_RS11320 0.55 -1.2 185,563 + HMPREF1078_RS11320 0.68 -4.0 185,563 + HMPREF1078_RS11320 0.68 -1.7 185,564 - HMPREF1078_RS11320 0.68 -0.3 185,626 + HMPREF1078_RS11320 0.85 -1.2 185,630 - HMPREF1078_RS11320 0.86 -1.6 185,974 - +0.2 185,999 - HMPREF1078_RS11335 0.27 -0.5 186,083 + -0.7 186,096 + +0.3 186,102 - +0.9 186,157 + +0.2 186,157 + -1.4 186,158 - +0.0 186,173 - +0.0 186,173 - -0.8 186,184 + -0.5 186,213 - +0.3
Or see this region's nucleotide sequence