Experiment: D-Mannose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS00125 and HMPREF1078_RS00130 overlap by 4 nucleotides HMPREF1078_RS00130 and HMPREF1078_RS00135 are separated by 10 nucleotides HMPREF1078_RS00135 and HMPREF1078_RS00140 are separated by 30 nucleotides HMPREF1078_RS00140 and HMPREF1078_RS00145 are separated by 29 nucleotides
HMPREF1078_RS00125: HMPREF1078_RS00125 - phosphoribosylanthranilate isomerase, at 29,183 to 29,848
_RS00125
HMPREF1078_RS00130: HMPREF1078_RS00130 - indole-3-glycerol phosphate synthase TrpC, at 29,845 to 30,654
_RS00130
HMPREF1078_RS00135: HMPREF1078_RS00135 - anthranilate phosphoribosyltransferase, at 30,665 to 31,669
_RS00135
HMPREF1078_RS00140: HMPREF1078_RS00140 - aminodeoxychorismate/anthranilate synthase component II, at 31,700 to 32,269
_RS00140
HMPREF1078_RS00145: HMPREF1078_RS00145 - anthranilate synthase component I family protein, at 32,299 to 33,702
_RS00145
Position (kb)
30
31
32 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 29.720 kb on + strand, within HMPREF1078_RS00125 at 29.720 kb on + strand, within HMPREF1078_RS00125 at 29.734 kb on + strand, within HMPREF1078_RS00125 at 29.738 kb on + strand, within HMPREF1078_RS00125 at 29.739 kb on - strand, within HMPREF1078_RS00125 at 29.766 kb on - strand, within HMPREF1078_RS00125 at 29.775 kb on - strand, within HMPREF1078_RS00125 at 29.851 kb on + strand at 29.852 kb on - strand at 29.861 kb on - strand at 29.887 kb on + strand at 29.887 kb on + strand at 29.990 kb on - strand, within HMPREF1078_RS00130 at 30.003 kb on + strand, within HMPREF1078_RS00130 at 30.067 kb on - strand, within HMPREF1078_RS00130 at 30.090 kb on + strand, within HMPREF1078_RS00130 at 30.106 kb on - strand, within HMPREF1078_RS00130 at 30.178 kb on - strand, within HMPREF1078_RS00130 at 30.198 kb on + strand, within HMPREF1078_RS00130 at 30.257 kb on - strand, within HMPREF1078_RS00130 at 30.350 kb on - strand, within HMPREF1078_RS00130 at 30.434 kb on - strand, within HMPREF1078_RS00130 at 30.435 kb on + strand, within HMPREF1078_RS00130 at 30.436 kb on - strand, within HMPREF1078_RS00130 at 30.451 kb on + strand, within HMPREF1078_RS00130 at 30.619 kb on + strand at 30.629 kb on - strand at 30.630 kb on + strand at 30.631 kb on - strand at 30.631 kb on - strand at 30.633 kb on + strand at 30.633 kb on + strand at 30.634 kb on - strand at 30.640 kb on + strand at 30.641 kb on - strand at 30.641 kb on - strand at 30.643 kb on + strand at 30.643 kb on + strand at 30.644 kb on - strand at 30.644 kb on - strand at 30.645 kb on + strand at 30.645 kb on + strand at 30.646 kb on - strand at 30.646 kb on - strand at 30.654 kb on + strand at 30.654 kb on + strand at 30.654 kb on + strand at 30.697 kb on + strand at 30.719 kb on - strand at 30.812 kb on + strand, within HMPREF1078_RS00135 at 30.945 kb on + strand, within HMPREF1078_RS00135 at 30.946 kb on - strand, within HMPREF1078_RS00135 at 31.019 kb on - strand, within HMPREF1078_RS00135 at 31.022 kb on + strand, within HMPREF1078_RS00135 at 31.023 kb on - strand, within HMPREF1078_RS00135 at 31.057 kb on + strand, within HMPREF1078_RS00135 at 31.057 kb on + strand, within HMPREF1078_RS00135 at 31.058 kb on - strand, within HMPREF1078_RS00135 at 31.058 kb on - strand, within HMPREF1078_RS00135 at 31.058 kb on - strand, within HMPREF1078_RS00135 at 31.058 kb on - strand, within HMPREF1078_RS00135 at 31.060 kb on - strand, within HMPREF1078_RS00135 at 31.060 kb on - strand, within HMPREF1078_RS00135 at 31.060 kb on - strand, within HMPREF1078_RS00135 at 31.060 kb on - strand, within HMPREF1078_RS00135 at 31.089 kb on + strand, within HMPREF1078_RS00135 at 31.090 kb on - strand, within HMPREF1078_RS00135 at 31.097 kb on + strand, within HMPREF1078_RS00135 at 31.108 kb on - strand, within HMPREF1078_RS00135 at 31.108 kb on - strand, within HMPREF1078_RS00135 at 31.227 kb on + strand, within HMPREF1078_RS00135 at 31.305 kb on - strand, within HMPREF1078_RS00135 at 31.360 kb on + strand, within HMPREF1078_RS00135 at 31.360 kb on + strand, within HMPREF1078_RS00135 at 31.403 kb on - strand, within HMPREF1078_RS00135 at 31.411 kb on + strand, within HMPREF1078_RS00135 at 31.529 kb on - strand, within HMPREF1078_RS00135 at 31.529 kb on - strand, within HMPREF1078_RS00135 at 31.531 kb on + strand, within HMPREF1078_RS00135 at 31.531 kb on + strand, within HMPREF1078_RS00135 at 31.532 kb on - strand, within HMPREF1078_RS00135 at 31.544 kb on + strand, within HMPREF1078_RS00135 at 31.576 kb on + strand at 31.577 kb on - strand at 31.594 kb on + strand at 31.632 kb on + strand at 31.633 kb on - strand at 31.647 kb on - strand at 31.651 kb on + strand at 31.652 kb on - strand at 31.652 kb on - strand at 31.652 kb on - strand at 31.652 kb on - strand at 31.652 kb on - strand at 31.652 kb on - strand at 31.653 kb on + strand at 31.654 kb on - strand at 31.654 kb on - strand at 31.655 kb on + strand at 31.655 kb on + strand at 31.655 kb on + strand at 31.655 kb on + strand at 31.655 kb on + strand at 31.655 kb on + strand at 31.655 kb on + strand at 31.655 kb on + strand at 31.655 kb on + strand at 31.655 kb on + strand at 31.655 kb on + strand at 31.655 kb on + strand at 31.656 kb on - strand at 31.656 kb on - strand at 31.656 kb on - strand at 31.656 kb on - strand at 31.656 kb on - strand at 31.656 kb on - strand at 31.656 kb on - strand at 31.656 kb on - strand at 31.656 kb on - strand at 31.658 kb on + strand at 31.659 kb on - strand at 31.659 kb on - strand at 31.789 kb on - strand, within HMPREF1078_RS00140 at 31.924 kb on + strand, within HMPREF1078_RS00140 at 31.952 kb on + strand, within HMPREF1078_RS00140 at 31.953 kb on - strand, within HMPREF1078_RS00140 at 31.953 kb on - strand, within HMPREF1078_RS00140 at 31.953 kb on - strand, within HMPREF1078_RS00140 at 31.953 kb on - strand, within HMPREF1078_RS00140 at 31.976 kb on + strand, within HMPREF1078_RS00140 at 31.976 kb on + strand, within HMPREF1078_RS00140 at 31.977 kb on - strand, within HMPREF1078_RS00140 at 31.977 kb on - strand, within HMPREF1078_RS00140 at 31.977 kb on - strand, within HMPREF1078_RS00140 at 31.977 kb on - strand, within HMPREF1078_RS00140 at 31.993 kb on + strand, within HMPREF1078_RS00140 at 32.184 kb on - strand, within HMPREF1078_RS00140 at 32.213 kb on + strand at 32.213 kb on + strand at 32.213 kb on + strand at 32.213 kb on + strand at 32.213 kb on + strand at 32.215 kb on + strand at 32.216 kb on - strand at 32.216 kb on - strand at 32.216 kb on - strand at 32.244 kb on + strand at 32.245 kb on - strand at 32.258 kb on + strand at 32.262 kb on - strand at 32.417 kb on + strand at 32.417 kb on + strand at 32.421 kb on - strand at 32.449 kb on - strand, within HMPREF1078_RS00145 at 32.449 kb on - strand, within HMPREF1078_RS00145 at 32.533 kb on + strand, within HMPREF1078_RS00145 at 32.560 kb on + strand, within HMPREF1078_RS00145 at 32.561 kb on - strand, within HMPREF1078_RS00145 at 32.561 kb on - strand, within HMPREF1078_RS00145 at 32.561 kb on - strand, within HMPREF1078_RS00145 at 32.598 kb on + strand, within HMPREF1078_RS00145 at 32.598 kb on + strand, within HMPREF1078_RS00145 at 32.598 kb on + strand, within HMPREF1078_RS00145 at 32.599 kb on - strand, within HMPREF1078_RS00145 at 32.599 kb on - strand, within HMPREF1078_RS00145 at 32.603 kb on - strand, within HMPREF1078_RS00145 at 32.603 kb on - strand, within HMPREF1078_RS00145 at 32.603 kb on - strand, within HMPREF1078_RS00145 at 32.604 kb on + strand, within HMPREF1078_RS00145 at 32.605 kb on - strand, within HMPREF1078_RS00145 at 32.606 kb on + strand, within HMPREF1078_RS00145 at 32.606 kb on + strand, within HMPREF1078_RS00145 at 32.606 kb on + strand, within HMPREF1078_RS00145 at 32.607 kb on - strand, within HMPREF1078_RS00145 at 32.607 kb on - strand, within HMPREF1078_RS00145
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannose 10 mg/mL (C) remove 29,720 + HMPREF1078_RS00125 0.81 -3.1 29,720 + HMPREF1078_RS00125 0.81 -2.9 29,734 + HMPREF1078_RS00125 0.83 -0.7 29,738 + HMPREF1078_RS00125 0.83 -3.2 29,739 - HMPREF1078_RS00125 0.83 -6.0 29,766 - HMPREF1078_RS00125 0.88 -4.0 29,775 - HMPREF1078_RS00125 0.89 -4.2 29,851 + -3.3 29,852 - -0.3 29,861 - +0.3 29,887 + -2.6 29,887 + -2.9 29,990 - HMPREF1078_RS00130 0.18 -3.7 30,003 + HMPREF1078_RS00130 0.20 -3.2 30,067 - HMPREF1078_RS00130 0.27 -2.2 30,090 + HMPREF1078_RS00130 0.30 -3.2 30,106 - HMPREF1078_RS00130 0.32 -3.4 30,178 - HMPREF1078_RS00130 0.41 -3.6 30,198 + HMPREF1078_RS00130 0.44 -2.1 30,257 - HMPREF1078_RS00130 0.51 -4.1 30,350 - HMPREF1078_RS00130 0.62 -3.3 30,434 - HMPREF1078_RS00130 0.73 -1.2 30,435 + HMPREF1078_RS00130 0.73 -3.4 30,436 - HMPREF1078_RS00130 0.73 -1.5 30,451 + HMPREF1078_RS00130 0.75 -2.3 30,619 + -2.9 30,629 - -3.0 30,630 + -3.2 30,631 - -2.8 30,631 - -4.2 30,633 + -1.4 30,633 + -1.8 30,634 - -3.6 30,640 + -3.2 30,641 - -1.8 30,641 - -3.3 30,643 + +0.0 30,643 + -3.3 30,644 - -2.4 30,644 - -3.8 30,645 + -2.4 30,645 + -1.0 30,646 - -2.7 30,646 - -1.9 30,654 + -3.6 30,654 + -3.0 30,654 + -1.0 30,697 + -3.1 30,719 - -3.8 30,812 + HMPREF1078_RS00135 0.15 -4.1 30,945 + HMPREF1078_RS00135 0.28 -3.5 30,946 - HMPREF1078_RS00135 0.28 -2.5 31,019 - HMPREF1078_RS00135 0.35 -3.4 31,022 + HMPREF1078_RS00135 0.36 -1.7 31,023 - HMPREF1078_RS00135 0.36 -3.7 31,057 + HMPREF1078_RS00135 0.39 -1.7 31,057 + HMPREF1078_RS00135 0.39 -1.1 31,058 - HMPREF1078_RS00135 0.39 -3.0 31,058 - HMPREF1078_RS00135 0.39 -4.8 31,058 - HMPREF1078_RS00135 0.39 -3.5 31,058 - HMPREF1078_RS00135 0.39 -3.3 31,060 - HMPREF1078_RS00135 0.39 -2.5 31,060 - HMPREF1078_RS00135 0.39 -2.2 31,060 - HMPREF1078_RS00135 0.39 -3.6 31,060 - HMPREF1078_RS00135 0.39 -0.5 31,089 + HMPREF1078_RS00135 0.42 -2.5 31,090 - HMPREF1078_RS00135 0.42 -4.1 31,097 + HMPREF1078_RS00135 0.43 -2.0 31,108 - HMPREF1078_RS00135 0.44 -4.6 31,108 - HMPREF1078_RS00135 0.44 -1.6 31,227 + HMPREF1078_RS00135 0.56 -0.8 31,305 - HMPREF1078_RS00135 0.64 -4.6 31,360 + HMPREF1078_RS00135 0.69 -3.1 31,360 + HMPREF1078_RS00135 0.69 -2.8 31,403 - HMPREF1078_RS00135 0.73 -1.8 31,411 + HMPREF1078_RS00135 0.74 -2.2 31,529 - HMPREF1078_RS00135 0.86 -1.0 31,529 - HMPREF1078_RS00135 0.86 -3.2 31,531 + HMPREF1078_RS00135 0.86 -4.2 31,531 + HMPREF1078_RS00135 0.86 -3.9 31,532 - HMPREF1078_RS00135 0.86 -2.9 31,544 + HMPREF1078_RS00135 0.87 -3.1 31,576 + -2.5 31,577 - -2.2 31,594 + -3.7 31,632 + -2.3 31,633 - -2.6 31,647 - +0.3 31,651 + -2.3 31,652 - -3.8 31,652 - -3.8 31,652 - -5.0 31,652 - -4.4 31,652 - -3.0 31,652 - -3.2 31,653 + -3.2 31,654 - -2.1 31,654 - -2.4 31,655 + -4.0 31,655 + -2.7 31,655 + -3.5 31,655 + -3.0 31,655 + +0.4 31,655 + -4.1 31,655 + -1.9 31,655 + -2.9 31,655 + -3.8 31,655 + -4.7 31,655 + -4.4 31,655 + -2.7 31,656 - -2.4 31,656 - -1.4 31,656 - +0.0 31,656 - -1.8 31,656 - -4.7 31,656 - -3.1 31,656 - -1.4 31,656 - -1.3 31,656 - -2.4 31,658 + -4.0 31,659 - -2.9 31,659 - -4.6 31,789 - HMPREF1078_RS00140 0.16 +0.2 31,924 + HMPREF1078_RS00140 0.39 -1.7 31,952 + HMPREF1078_RS00140 0.44 -3.5 31,953 - HMPREF1078_RS00140 0.44 -0.7 31,953 - HMPREF1078_RS00140 0.44 +0.0 31,953 - HMPREF1078_RS00140 0.44 -1.3 31,953 - HMPREF1078_RS00140 0.44 -0.2 31,976 + HMPREF1078_RS00140 0.48 -3.1 31,976 + HMPREF1078_RS00140 0.48 -2.0 31,977 - HMPREF1078_RS00140 0.49 -2.1 31,977 - HMPREF1078_RS00140 0.49 -1.3 31,977 - HMPREF1078_RS00140 0.49 -0.4 31,977 - HMPREF1078_RS00140 0.49 +0.1 31,993 + HMPREF1078_RS00140 0.51 -3.1 32,184 - HMPREF1078_RS00140 0.85 -2.1 32,213 + -3.0 32,213 + -1.7 32,213 + -0.7 32,213 + -3.4 32,213 + -1.9 32,215 + -4.3 32,216 - +1.5 32,216 - -2.2 32,216 - -1.9 32,244 + +0.8 32,245 - -2.9 32,258 + -3.4 32,262 - -1.2 32,417 + -1.6 32,417 + -3.5 32,421 - -0.2 32,449 - HMPREF1078_RS00145 0.11 -4.2 32,449 - HMPREF1078_RS00145 0.11 -4.3 32,533 + HMPREF1078_RS00145 0.17 -2.8 32,560 + HMPREF1078_RS00145 0.19 -3.2 32,561 - HMPREF1078_RS00145 0.19 -2.3 32,561 - HMPREF1078_RS00145 0.19 -4.2 32,561 - HMPREF1078_RS00145 0.19 -4.3 32,598 + HMPREF1078_RS00145 0.21 -3.4 32,598 + HMPREF1078_RS00145 0.21 -2.6 32,598 + HMPREF1078_RS00145 0.21 -2.0 32,599 - HMPREF1078_RS00145 0.21 -3.1 32,599 - HMPREF1078_RS00145 0.21 +1.3 32,603 - HMPREF1078_RS00145 0.22 -2.5 32,603 - HMPREF1078_RS00145 0.22 -2.6 32,603 - HMPREF1078_RS00145 0.22 -2.9 32,604 + HMPREF1078_RS00145 0.22 -3.2 32,605 - HMPREF1078_RS00145 0.22 -2.7 32,606 + HMPREF1078_RS00145 0.22 -1.1 32,606 + HMPREF1078_RS00145 0.22 +0.0 32,606 + HMPREF1078_RS00145 0.22 -3.2 32,607 - HMPREF1078_RS00145 0.22 -2.8 32,607 - HMPREF1078_RS00145 0.22 -2.5
Or see this region's nucleotide sequence