Experiment: D-Mannose 10 mg/mL (C) 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt HMPREF1078_RS00120 and HMPREF1078_RS00125 are separated by 22 nucleotides HMPREF1078_RS00125 and HMPREF1078_RS00130 overlap by 4 nucleotides HMPREF1078_RS00130 and HMPREF1078_RS00135 are separated by 10 nucleotides 
        HMPREF1078_RS00120: HMPREF1078_RS00120 - tryptophan synthase subunit alpha, at 28,390 to 29,160 
        _RS00120 
         
        
        HMPREF1078_RS00125: HMPREF1078_RS00125 - phosphoribosylanthranilate isomerase, at 29,183 to 29,848 
        _RS00125 
         
        
        HMPREF1078_RS00130: HMPREF1078_RS00130 - indole-3-glycerol phosphate synthase TrpC, at 29,845 to 30,654 
        _RS00130 
         
        
        HMPREF1078_RS00135: HMPREF1078_RS00135 - anthranilate phosphoribosyltransferase, at 30,665 to 31,669 
        _RS00135 
         Position (kb) 29 
30 Strain fitness (log2 ratio) -6 
-5 
-4 
-3 
-2 
-1 
0 
1 at 28.196 kb on + strand at 28.206 kb on + strand at 28.225 kb on - strand at 28.272 kb on + strand at 28.289 kb on - strand at 28.324 kb on - strand at 28.450 kb on + strand at 28.471 kb on + strand, within HMPREF1078_RS00120 at 28.472 kb on - strand, within HMPREF1078_RS00120 at 28.472 kb on - strand, within HMPREF1078_RS00120 at 28.472 kb on - strand, within HMPREF1078_RS00120 at 28.492 kb on - strand, within HMPREF1078_RS00120 at 28.494 kb on + strand, within HMPREF1078_RS00120 at 28.498 kb on + strand, within HMPREF1078_RS00120 at 28.499 kb on - strand, within HMPREF1078_RS00120 at 28.499 kb on - strand, within HMPREF1078_RS00120 at 28.640 kb on - strand, within HMPREF1078_RS00120 at 28.646 kb on + strand, within HMPREF1078_RS00120 at 28.647 kb on - strand, within HMPREF1078_RS00120 at 28.662 kb on + strand, within HMPREF1078_RS00120 at 28.662 kb on + strand, within HMPREF1078_RS00120 at 28.663 kb on - strand, within HMPREF1078_RS00120 at 28.663 kb on - strand, within HMPREF1078_RS00120 at 28.663 kb on - strand, within HMPREF1078_RS00120 at 28.716 kb on + strand, within HMPREF1078_RS00120 at 28.734 kb on - strand, within HMPREF1078_RS00120 at 28.754 kb on + strand, within HMPREF1078_RS00120 at 28.764 kb on + strand, within HMPREF1078_RS00120 at 28.766 kb on + strand, within HMPREF1078_RS00120 at 28.767 kb on - strand, within HMPREF1078_RS00120 at 28.767 kb on - strand, within HMPREF1078_RS00120 at 28.767 kb on - strand, within HMPREF1078_RS00120 at 28.767 kb on - strand, within HMPREF1078_RS00120 at 28.767 kb on - strand, within HMPREF1078_RS00120 at 28.804 kb on + strand, within HMPREF1078_RS00120 at 28.848 kb on - strand, within HMPREF1078_RS00120 at 28.909 kb on - strand, within HMPREF1078_RS00120 at 28.915 kb on + strand, within HMPREF1078_RS00120 at 29.027 kb on - strand, within HMPREF1078_RS00120 at 29.035 kb on + strand, within HMPREF1078_RS00120 at 29.035 kb on + strand, within HMPREF1078_RS00120 at 29.035 kb on - strand, within HMPREF1078_RS00120 at 29.036 kb on - strand, within HMPREF1078_RS00120 at 29.050 kb on + strand, within HMPREF1078_RS00120 at 29.059 kb on + strand, within HMPREF1078_RS00120 at 29.059 kb on + strand, within HMPREF1078_RS00120 at 29.223 kb on - strand at 29.231 kb on + strand at 29.232 kb on - strand at 29.309 kb on + strand, within HMPREF1078_RS00125 at 29.309 kb on + strand, within HMPREF1078_RS00125 at 29.310 kb on - strand, within HMPREF1078_RS00125 at 29.416 kb on - strand, within HMPREF1078_RS00125 at 29.419 kb on + strand, within HMPREF1078_RS00125 at 29.420 kb on - strand, within HMPREF1078_RS00125 at 29.662 kb on + strand, within HMPREF1078_RS00125 at 29.720 kb on + strand, within HMPREF1078_RS00125 at 29.720 kb on + strand, within HMPREF1078_RS00125 at 29.734 kb on + strand, within HMPREF1078_RS00125 at 29.738 kb on + strand, within HMPREF1078_RS00125 at 29.739 kb on - strand, within HMPREF1078_RS00125 at 29.766 kb on - strand, within HMPREF1078_RS00125 at 29.775 kb on - strand, within HMPREF1078_RS00125 at 29.851 kb on + strand at 29.852 kb on - strand at 29.861 kb on - strand at 29.887 kb on + strand at 29.887 kb on + strand at 29.990 kb on - strand, within HMPREF1078_RS00130 at 30.003 kb on + strand, within HMPREF1078_RS00130 at 30.067 kb on - strand, within HMPREF1078_RS00130 at 30.090 kb on + strand, within HMPREF1078_RS00130 at 30.106 kb on - strand, within HMPREF1078_RS00130 at 30.178 kb on - strand, within HMPREF1078_RS00130 at 30.198 kb on + strand, within HMPREF1078_RS00130 at 30.257 kb on - strand, within HMPREF1078_RS00130 at 30.350 kb on - strand, within HMPREF1078_RS00130 at 30.434 kb on - strand, within HMPREF1078_RS00130 at 30.435 kb on + strand, within HMPREF1078_RS00130 at 30.436 kb on - strand, within HMPREF1078_RS00130 at 30.451 kb on + strand, within HMPREF1078_RS00130 at 30.619 kb on + strand at 30.629 kb on - strand at 30.630 kb on + strand at 30.631 kb on - strand at 30.631 kb on - strand at 30.633 kb on + strand at 30.633 kb on + strand at 30.634 kb on - strand at 30.640 kb on + strand at 30.641 kb on - strand at 30.641 kb on - strand at 30.643 kb on + strand at 30.643 kb on + strand at 30.644 kb on - strand at 30.644 kb on - strand at 30.645 kb on + strand at 30.645 kb on + strand at 30.646 kb on - strand at 30.646 kb on - strand at 30.654 kb on + strand at 30.654 kb on + strand at 30.654 kb on + strand at 30.697 kb on + strand at 30.719 kb on - strand at 30.812 kb on + strand, within HMPREF1078_RS00135  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction D-Mannose 10 mg/mL (C) remove 28,196 +  +0.7 28,206 +  -0.9 28,225 -  -0.4 28,272 +  +0.8 28,289 -  +0.1 28,324 -  -0.5 28,450 +  -1.1 28,471 +  HMPREF1078_RS00120 0.11  -1.6 28,472 -  HMPREF1078_RS00120 0.11  -1.4 28,472 -  HMPREF1078_RS00120 0.11  -0.8 28,472 -  HMPREF1078_RS00120 0.11  -3.1 28,492 -  HMPREF1078_RS00120 0.13  -3.5 28,494 +  HMPREF1078_RS00120 0.13  -4.1 28,498 +  HMPREF1078_RS00120 0.14  -2.3 28,499 -  HMPREF1078_RS00120 0.14  -3.3 28,499 -  HMPREF1078_RS00120 0.14  -2.6 28,640 -  HMPREF1078_RS00120 0.32  -3.2 28,646 +  HMPREF1078_RS00120 0.33  -0.2 28,647 -  HMPREF1078_RS00120 0.33  -0.7 28,662 +  HMPREF1078_RS00120 0.35  -3.2 28,662 +  HMPREF1078_RS00120 0.35  -1.5 28,663 -  HMPREF1078_RS00120 0.35  -3.2 28,663 -  HMPREF1078_RS00120 0.35  -2.8 28,663 -  HMPREF1078_RS00120 0.35  -1.1 28,716 +  HMPREF1078_RS00120 0.42  -3.9 28,734 -  HMPREF1078_RS00120 0.45  -3.5 28,754 +  HMPREF1078_RS00120 0.47  -2.5 28,764 +  HMPREF1078_RS00120 0.49  -3.8 28,766 +  HMPREF1078_RS00120 0.49  -2.5 28,767 -  HMPREF1078_RS00120 0.49  -4.3 28,767 -  HMPREF1078_RS00120 0.49  -1.9 28,767 -  HMPREF1078_RS00120 0.49  -1.2 28,767 -  HMPREF1078_RS00120 0.49  -2.4 28,767 -  HMPREF1078_RS00120 0.49  -4.3 28,804 +  HMPREF1078_RS00120 0.54  -3.8 28,848 -  HMPREF1078_RS00120 0.59  -5.4 28,909 -  HMPREF1078_RS00120 0.67  -3.6 28,915 +  HMPREF1078_RS00120 0.68  -4.5 29,027 -  HMPREF1078_RS00120 0.83  -2.5 29,035 +  HMPREF1078_RS00120 0.84  -4.1 29,035 +  HMPREF1078_RS00120 0.84  -1.5 29,035 -  HMPREF1078_RS00120 0.84  -3.9 29,036 -  HMPREF1078_RS00120 0.84  -0.5 29,050 +  HMPREF1078_RS00120 0.86  -4.5 29,059 +  HMPREF1078_RS00120 0.87  -2.2 29,059 +  HMPREF1078_RS00120 0.87  -3.0 29,223 -  -3.2 29,231 +  -3.9 29,232 -  -3.0 29,309 +  HMPREF1078_RS00125 0.19  -1.0 29,309 +  HMPREF1078_RS00125 0.19  -2.0 29,310 -  HMPREF1078_RS00125 0.19  -3.8 29,416 -  HMPREF1078_RS00125 0.35  -4.5 29,419 +  HMPREF1078_RS00125 0.35  -0.8 29,420 -  HMPREF1078_RS00125 0.36  -3.7 29,662 +  HMPREF1078_RS00125 0.72  -3.9 29,720 +  HMPREF1078_RS00125 0.81  -3.1 29,720 +  HMPREF1078_RS00125 0.81  -2.9 29,734 +  HMPREF1078_RS00125 0.83  -0.7 29,738 +  HMPREF1078_RS00125 0.83  -3.2 29,739 -  HMPREF1078_RS00125 0.83  -6.0 29,766 -  HMPREF1078_RS00125 0.88  -4.0 29,775 -  HMPREF1078_RS00125 0.89  -4.2 29,851 +  -3.3 29,852 -  -0.3 29,861 -  +0.3 29,887 +  -2.6 29,887 +  -2.9 29,990 -  HMPREF1078_RS00130 0.18  -3.7 30,003 +  HMPREF1078_RS00130 0.20  -3.2 30,067 -  HMPREF1078_RS00130 0.27  -2.2 30,090 +  HMPREF1078_RS00130 0.30  -3.2 30,106 -  HMPREF1078_RS00130 0.32  -3.4 30,178 -  HMPREF1078_RS00130 0.41  -3.6 30,198 +  HMPREF1078_RS00130 0.44  -2.1 30,257 -  HMPREF1078_RS00130 0.51  -4.1 30,350 -  HMPREF1078_RS00130 0.62  -3.3 30,434 -  HMPREF1078_RS00130 0.73  -1.2 30,435 +  HMPREF1078_RS00130 0.73  -3.4 30,436 -  HMPREF1078_RS00130 0.73  -1.5 30,451 +  HMPREF1078_RS00130 0.75  -2.3 30,619 +  -2.9 30,629 -  -3.0 30,630 +  -3.2 30,631 -  -2.8 30,631 -  -4.2 30,633 +  -1.4 30,633 +  -1.8 30,634 -  -3.6 30,640 +  -3.2 30,641 -  -1.8 30,641 -  -3.3 30,643 +  +0.0 30,643 +  -3.3 30,644 -  -2.4 30,644 -  -3.8 30,645 +  -2.4 30,645 +  -1.0 30,646 -  -2.7 30,646 -  -1.9 30,654 +  -3.6 30,654 +  -3.0 30,654 +  -1.0 30,697 +  -3.1 30,719 -  -3.8 30,812 +  HMPREF1078_RS00135 0.15  -4.1 
Or see this region's nucleotide sequence