Experiment: Laminarin from Laminaria digitata 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS03470 and HMPREF1078_RS03475 overlap by 4 nucleotides HMPREF1078_RS03475 and HMPREF1078_RS03480 overlap by 10 nucleotides HMPREF1078_RS03480 and HMPREF1078_RS03485 are separated by 21 nucleotides
HMPREF1078_RS03470: HMPREF1078_RS03470 - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 820,033 to 820,872
_RS03470
HMPREF1078_RS03475: HMPREF1078_RS03475 - thiamine phosphate synthase, at 820,869 to 821,468
_RS03475
HMPREF1078_RS03480: HMPREF1078_RS03480 - thiamine phosphate synthase, at 821,459 to 822,196
_RS03480
HMPREF1078_RS03485: HMPREF1078_RS03485 - phosphomethylpyrimidine synthase ThiC, at 822,218 to 823,924
_RS03485
Position (kb) 820
821
822 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 819.879 kb on + strand at 819.890 kb on + strand at 819.953 kb on - strand at 819.954 kb on + strand at 819.955 kb on - strand at 819.955 kb on - strand at 819.955 kb on - strand at 820.024 kb on + strand at 820.025 kb on - strand at 820.028 kb on + strand at 820.041 kb on - strand at 820.071 kb on + strand at 820.071 kb on + strand at 820.071 kb on + strand at 820.071 kb on + strand at 820.072 kb on - strand at 820.074 kb on + strand at 820.075 kb on - strand at 820.075 kb on - strand at 820.075 kb on - strand at 820.109 kb on + strand at 820.109 kb on + strand at 820.197 kb on - strand, within HMPREF1078_RS03470 at 820.206 kb on - strand, within HMPREF1078_RS03470 at 820.206 kb on - strand, within HMPREF1078_RS03470 at 820.262 kb on + strand, within HMPREF1078_RS03470 at 820.297 kb on - strand, within HMPREF1078_RS03470 at 820.371 kb on + strand, within HMPREF1078_RS03470 at 820.401 kb on - strand, within HMPREF1078_RS03470 at 820.402 kb on + strand, within HMPREF1078_RS03470 at 820.503 kb on - strand, within HMPREF1078_RS03470 at 820.672 kb on - strand, within HMPREF1078_RS03470 at 820.715 kb on - strand, within HMPREF1078_RS03470 at 820.716 kb on + strand, within HMPREF1078_RS03470 at 820.748 kb on + strand, within HMPREF1078_RS03470 at 820.749 kb on - strand, within HMPREF1078_RS03470 at 820.749 kb on - strand, within HMPREF1078_RS03470 at 820.869 kb on - strand at 820.889 kb on + strand at 821.028 kb on + strand, within HMPREF1078_RS03475 at 821.071 kb on + strand, within HMPREF1078_RS03475 at 821.072 kb on - strand, within HMPREF1078_RS03475 at 821.077 kb on - strand, within HMPREF1078_RS03475 at 821.197 kb on - strand, within HMPREF1078_RS03475 at 821.227 kb on + strand, within HMPREF1078_RS03475 at 821.317 kb on + strand, within HMPREF1078_RS03475 at 821.317 kb on + strand, within HMPREF1078_RS03475 at 821.318 kb on - strand, within HMPREF1078_RS03475 at 821.327 kb on - strand, within HMPREF1078_RS03475 at 821.327 kb on - strand, within HMPREF1078_RS03475 at 821.350 kb on + strand, within HMPREF1078_RS03475 at 821.417 kb on + strand at 821.430 kb on - strand at 821.443 kb on - strand at 821.444 kb on + strand at 821.444 kb on + strand at 821.444 kb on + strand at 821.445 kb on - strand at 821.504 kb on + strand at 821.505 kb on - strand at 821.574 kb on - strand, within HMPREF1078_RS03480 at 821.605 kb on + strand, within HMPREF1078_RS03480 at 821.605 kb on + strand, within HMPREF1078_RS03480 at 821.800 kb on + strand, within HMPREF1078_RS03480 at 821.894 kb on + strand, within HMPREF1078_RS03480 at 821.894 kb on + strand, within HMPREF1078_RS03480 at 821.895 kb on - strand, within HMPREF1078_RS03480 at 821.895 kb on - strand, within HMPREF1078_RS03480 at 821.895 kb on - strand, within HMPREF1078_RS03480 at 821.898 kb on + strand, within HMPREF1078_RS03480 at 821.899 kb on - strand, within HMPREF1078_RS03480 at 821.919 kb on - strand, within HMPREF1078_RS03480 at 822.072 kb on + strand, within HMPREF1078_RS03480 at 822.161 kb on - strand at 822.194 kb on + strand at 822.195 kb on + strand at 822.217 kb on + strand at 822.218 kb on - strand at 822.218 kb on - strand at 822.237 kb on + strand at 822.237 kb on + strand at 822.248 kb on + strand at 822.269 kb on + strand at 822.285 kb on + strand at 822.285 kb on + strand at 822.285 kb on + strand at 822.286 kb on - strand at 822.286 kb on - strand at 822.389 kb on + strand, within HMPREF1078_RS03485 at 822.389 kb on + strand, within HMPREF1078_RS03485 at 822.389 kb on + strand, within HMPREF1078_RS03485 at 822.394 kb on + strand, within HMPREF1078_RS03485 at 822.407 kb on + strand, within HMPREF1078_RS03485 at 822.433 kb on + strand, within HMPREF1078_RS03485 at 822.433 kb on + strand, within HMPREF1078_RS03485 at 822.433 kb on + strand, within HMPREF1078_RS03485
Per-strain Table
Position Strand Gene LocusTag Fraction Laminarin from Laminaria digitata 2.5 mg/mL (C) remove 819,879 + -2.3 819,890 + -2.9 819,953 - -0.5 819,954 + -2.4 819,955 - -2.4 819,955 - +0.7 819,955 - -2.4 820,024 + -0.8 820,025 - +0.8 820,028 + -3.6 820,041 - -1.2 820,071 + -1.8 820,071 + -2.1 820,071 + +0.2 820,071 + -2.4 820,072 - -0.6 820,074 + +1.2 820,075 - -0.4 820,075 - +1.3 820,075 - +0.6 820,109 + +1.5 820,109 + +0.4 820,197 - HMPREF1078_RS03470 0.20 -0.8 820,206 - HMPREF1078_RS03470 0.21 +0.3 820,206 - HMPREF1078_RS03470 0.21 +1.6 820,262 + HMPREF1078_RS03470 0.27 -0.2 820,297 - HMPREF1078_RS03470 0.31 +1.6 820,371 + HMPREF1078_RS03470 0.40 +0.2 820,401 - HMPREF1078_RS03470 0.44 -0.4 820,402 + HMPREF1078_RS03470 0.44 -0.6 820,503 - HMPREF1078_RS03470 0.56 -2.1 820,672 - HMPREF1078_RS03470 0.76 -2.7 820,715 - HMPREF1078_RS03470 0.81 -1.3 820,716 + HMPREF1078_RS03470 0.81 -0.7 820,748 + HMPREF1078_RS03470 0.85 +1.9 820,749 - HMPREF1078_RS03470 0.85 -0.5 820,749 - HMPREF1078_RS03470 0.85 +1.0 820,869 - +0.9 820,889 + +0.4 821,028 + HMPREF1078_RS03475 0.27 +0.4 821,071 + HMPREF1078_RS03475 0.34 -0.1 821,072 - HMPREF1078_RS03475 0.34 -2.7 821,077 - HMPREF1078_RS03475 0.35 -0.8 821,197 - HMPREF1078_RS03475 0.55 +0.4 821,227 + HMPREF1078_RS03475 0.60 -1.1 821,317 + HMPREF1078_RS03475 0.75 -0.5 821,317 + HMPREF1078_RS03475 0.75 -1.6 821,318 - HMPREF1078_RS03475 0.75 -3.2 821,327 - HMPREF1078_RS03475 0.76 -0.4 821,327 - HMPREF1078_RS03475 0.76 -0.4 821,350 + HMPREF1078_RS03475 0.80 +0.3 821,417 + +1.4 821,430 - -2.1 821,443 - -0.7 821,444 + -1.7 821,444 + +2.7 821,444 + +1.6 821,445 - -0.8 821,504 + -1.4 821,505 - +1.3 821,574 - HMPREF1078_RS03480 0.16 -1.2 821,605 + HMPREF1078_RS03480 0.20 -1.4 821,605 + HMPREF1078_RS03480 0.20 +1.8 821,800 + HMPREF1078_RS03480 0.46 +0.1 821,894 + HMPREF1078_RS03480 0.59 -0.0 821,894 + HMPREF1078_RS03480 0.59 -0.7 821,895 - HMPREF1078_RS03480 0.59 -0.7 821,895 - HMPREF1078_RS03480 0.59 +0.4 821,895 - HMPREF1078_RS03480 0.59 -0.9 821,898 + HMPREF1078_RS03480 0.59 +1.5 821,899 - HMPREF1078_RS03480 0.60 +1.3 821,919 - HMPREF1078_RS03480 0.62 -0.9 822,072 + HMPREF1078_RS03480 0.83 -0.6 822,161 - -0.8 822,194 + -1.9 822,195 + +0.3 822,217 + -2.6 822,218 - -2.9 822,218 - -1.3 822,237 + +0.1 822,237 + +0.4 822,248 + -3.3 822,269 + -0.7 822,285 + -0.3 822,285 + -1.5 822,285 + -0.3 822,286 - -1.1 822,286 - -0.6 822,389 + HMPREF1078_RS03485 0.10 +0.3 822,389 + HMPREF1078_RS03485 0.10 -1.6 822,389 + HMPREF1078_RS03485 0.10 -3.2 822,394 + HMPREF1078_RS03485 0.10 -1.3 822,407 + HMPREF1078_RS03485 0.11 +0.7 822,433 + HMPREF1078_RS03485 0.13 -1.5 822,433 + HMPREF1078_RS03485 0.13 -0.7 822,433 + HMPREF1078_RS03485 0.13 -2.9
Or see this region's nucleotide sequence