Experiment: Pectin from citrus peel; autoclaved 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS17210 and HMPREF1078_RS17215 are separated by 294 nucleotides HMPREF1078_RS17215 and HMPREF1078_RS17220 overlap by 4 nucleotides HMPREF1078_RS17220 and HMPREF1078_RS17225 are separated by 109 nucleotides
HMPREF1078_RS17210: HMPREF1078_RS17210 - ABC transporter ATP-binding protein, at 158,569 to 160,416
_RS17210
HMPREF1078_RS17215: HMPREF1078_RS17215 - radical SAM-associated putative lipoprotein, at 160,711 to 161,187
_RS17215
HMPREF1078_RS17220: HMPREF1078_RS17220 - TIGR04133 family radical SAM/SPASM protein, at 161,184 to 162,257
_RS17220
HMPREF1078_RS17225: HMPREF1078_RS17225 - DUF4091 domain-containing protein, at 162,367 to 164,142
_RS17225
Position (kb) 161
162
163 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 160.506 kb on + strand at 160.556 kb on + strand at 160.557 kb on - strand at 160.557 kb on - strand at 160.617 kb on + strand at 160.625 kb on + strand at 160.626 kb on - strand at 160.627 kb on + strand at 160.630 kb on + strand at 160.633 kb on - strand at 160.671 kb on - strand at 160.672 kb on + strand at 160.685 kb on - strand at 160.686 kb on + strand at 160.689 kb on + strand at 160.704 kb on + strand at 160.736 kb on + strand at 160.769 kb on + strand, within HMPREF1078_RS17215 at 160.769 kb on + strand, within HMPREF1078_RS17215 at 160.770 kb on - strand, within HMPREF1078_RS17215 at 160.770 kb on - strand, within HMPREF1078_RS17215 at 160.770 kb on - strand, within HMPREF1078_RS17215 at 160.819 kb on + strand, within HMPREF1078_RS17215 at 160.819 kb on + strand, within HMPREF1078_RS17215 at 160.820 kb on - strand, within HMPREF1078_RS17215 at 160.820 kb on - strand, within HMPREF1078_RS17215 at 160.824 kb on + strand, within HMPREF1078_RS17215 at 160.824 kb on + strand, within HMPREF1078_RS17215 at 161.027 kb on + strand, within HMPREF1078_RS17215 at 161.039 kb on + strand, within HMPREF1078_RS17215 at 161.039 kb on + strand, within HMPREF1078_RS17215 at 161.062 kb on + strand, within HMPREF1078_RS17215 at 161.085 kb on + strand, within HMPREF1078_RS17215 at 161.154 kb on - strand at 161.222 kb on - strand at 161.222 kb on - strand at 161.227 kb on + strand at 161.420 kb on + strand, within HMPREF1078_RS17220 at 161.796 kb on - strand, within HMPREF1078_RS17220 at 161.911 kb on + strand, within HMPREF1078_RS17220 at 161.912 kb on - strand, within HMPREF1078_RS17220 at 162.026 kb on + strand, within HMPREF1078_RS17220 at 162.026 kb on + strand, within HMPREF1078_RS17220 at 162.027 kb on - strand, within HMPREF1078_RS17220 at 162.072 kb on - strand, within HMPREF1078_RS17220 at 162.121 kb on - strand, within HMPREF1078_RS17220 at 162.342 kb on + strand at 162.381 kb on + strand at 162.381 kb on + strand at 162.466 kb on - strand at 162.624 kb on + strand, within HMPREF1078_RS17225 at 162.624 kb on + strand, within HMPREF1078_RS17225 at 162.625 kb on - strand, within HMPREF1078_RS17225 at 162.625 kb on - strand, within HMPREF1078_RS17225 at 162.638 kb on + strand, within HMPREF1078_RS17225 at 162.652 kb on - strand, within HMPREF1078_RS17225 at 162.671 kb on + strand, within HMPREF1078_RS17225 at 162.672 kb on - strand, within HMPREF1078_RS17225 at 162.743 kb on + strand, within HMPREF1078_RS17225 at 162.744 kb on - strand, within HMPREF1078_RS17225 at 162.748 kb on + strand, within HMPREF1078_RS17225 at 162.748 kb on + strand, within HMPREF1078_RS17225 at 162.749 kb on - strand, within HMPREF1078_RS17225 at 162.749 kb on - strand, within HMPREF1078_RS17225 at 162.749 kb on - strand, within HMPREF1078_RS17225 at 162.849 kb on + strand, within HMPREF1078_RS17225 at 162.850 kb on - strand, within HMPREF1078_RS17225 at 162.906 kb on - strand, within HMPREF1078_RS17225 at 162.906 kb on - strand, within HMPREF1078_RS17225 at 162.937 kb on + strand, within HMPREF1078_RS17225 at 162.937 kb on + strand, within HMPREF1078_RS17225 at 162.938 kb on - strand, within HMPREF1078_RS17225 at 162.938 kb on - strand, within HMPREF1078_RS17225 at 162.938 kb on - strand, within HMPREF1078_RS17225 at 162.939 kb on + strand, within HMPREF1078_RS17225 at 162.978 kb on + strand, within HMPREF1078_RS17225 at 162.993 kb on - strand, within HMPREF1078_RS17225 at 163.242 kb on + strand, within HMPREF1078_RS17225
Per-strain Table
Position Strand Gene LocusTag Fraction Pectin from citrus peel; autoclaved 2.5 mg/mL (C) remove 160,506 + +0.2 160,556 + -1.5 160,557 - -0.0 160,557 - +1.0 160,617 + +0.9 160,625 + -1.2 160,626 - -2.2 160,627 + +0.2 160,630 + -1.0 160,633 - -1.2 160,671 - +1.6 160,672 + +1.4 160,685 - -2.8 160,686 + -0.6 160,689 + -0.1 160,704 + +1.5 160,736 + -0.7 160,769 + HMPREF1078_RS17215 0.12 +0.2 160,769 + HMPREF1078_RS17215 0.12 +1.4 160,770 - HMPREF1078_RS17215 0.12 +0.4 160,770 - HMPREF1078_RS17215 0.12 -1.4 160,770 - HMPREF1078_RS17215 0.12 -1.1 160,819 + HMPREF1078_RS17215 0.23 -0.5 160,819 + HMPREF1078_RS17215 0.23 +0.1 160,820 - HMPREF1078_RS17215 0.23 -2.5 160,820 - HMPREF1078_RS17215 0.23 +0.9 160,824 + HMPREF1078_RS17215 0.24 -0.3 160,824 + HMPREF1078_RS17215 0.24 +0.3 161,027 + HMPREF1078_RS17215 0.66 -3.7 161,039 + HMPREF1078_RS17215 0.69 -0.1 161,039 + HMPREF1078_RS17215 0.69 -2.6 161,062 + HMPREF1078_RS17215 0.74 -0.3 161,085 + HMPREF1078_RS17215 0.78 -0.7 161,154 - -0.9 161,222 - -0.3 161,222 - +1.8 161,227 + +0.0 161,420 + HMPREF1078_RS17220 0.22 -0.6 161,796 - HMPREF1078_RS17220 0.57 -2.0 161,911 + HMPREF1078_RS17220 0.68 -0.5 161,912 - HMPREF1078_RS17220 0.68 +0.5 162,026 + HMPREF1078_RS17220 0.78 -1.4 162,026 + HMPREF1078_RS17220 0.78 +0.5 162,027 - HMPREF1078_RS17220 0.78 +0.7 162,072 - HMPREF1078_RS17220 0.83 +1.8 162,121 - HMPREF1078_RS17220 0.87 -0.5 162,342 + +1.7 162,381 + +2.0 162,381 + -0.1 162,466 - +0.5 162,624 + HMPREF1078_RS17225 0.14 -0.5 162,624 + HMPREF1078_RS17225 0.14 -1.5 162,625 - HMPREF1078_RS17225 0.15 +0.9 162,625 - HMPREF1078_RS17225 0.15 -1.4 162,638 + HMPREF1078_RS17225 0.15 +2.5 162,652 - HMPREF1078_RS17225 0.16 -2.4 162,671 + HMPREF1078_RS17225 0.17 -0.8 162,672 - HMPREF1078_RS17225 0.17 -1.5 162,743 + HMPREF1078_RS17225 0.21 +0.6 162,744 - HMPREF1078_RS17225 0.21 +0.6 162,748 + HMPREF1078_RS17225 0.21 -0.7 162,748 + HMPREF1078_RS17225 0.21 -2.3 162,749 - HMPREF1078_RS17225 0.22 -2.1 162,749 - HMPREF1078_RS17225 0.22 -1.5 162,749 - HMPREF1078_RS17225 0.22 +0.3 162,849 + HMPREF1078_RS17225 0.27 -0.4 162,850 - HMPREF1078_RS17225 0.27 -1.8 162,906 - HMPREF1078_RS17225 0.30 -0.8 162,906 - HMPREF1078_RS17225 0.30 +0.2 162,937 + HMPREF1078_RS17225 0.32 -0.0 162,937 + HMPREF1078_RS17225 0.32 +1.6 162,938 - HMPREF1078_RS17225 0.32 -0.3 162,938 - HMPREF1078_RS17225 0.32 +0.8 162,938 - HMPREF1078_RS17225 0.32 -0.4 162,939 + HMPREF1078_RS17225 0.32 +1.4 162,978 + HMPREF1078_RS17225 0.34 +0.0 162,993 - HMPREF1078_RS17225 0.35 +0.9 163,242 + HMPREF1078_RS17225 0.49 +0.4
Or see this region's nucleotide sequence