Experiment: Pectin from citrus peel; autoclaved 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS05910 and HMPREF1078_RS05915 are separated by 271 nucleotides HMPREF1078_RS05915 and HMPREF1078_RS05920 are separated by 197 nucleotides
HMPREF1078_RS05910: HMPREF1078_RS05910 - nucleoside kinase, at 514,426 to 516,093
_RS05910
HMPREF1078_RS05915: HMPREF1078_RS05915 - ATP-binding protein, at 516,365 to 518,527
_RS05915
HMPREF1078_RS05920: HMPREF1078_RS05920 - RNA polymerase factor sigma-54, at 518,725 to 520,194
_RS05920
Position (kb) 516
517
518
519 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 515.455 kb on + strand, within HMPREF1078_RS05910 at 515.486 kb on + strand, within HMPREF1078_RS05910 at 515.491 kb on + strand, within HMPREF1078_RS05910 at 515.621 kb on + strand, within HMPREF1078_RS05910 at 515.622 kb on - strand, within HMPREF1078_RS05910 at 515.674 kb on + strand, within HMPREF1078_RS05910 at 515.676 kb on + strand, within HMPREF1078_RS05910 at 515.689 kb on + strand, within HMPREF1078_RS05910 at 515.689 kb on + strand, within HMPREF1078_RS05910 at 515.727 kb on + strand, within HMPREF1078_RS05910 at 515.728 kb on - strand, within HMPREF1078_RS05910 at 515.728 kb on - strand, within HMPREF1078_RS05910 at 515.903 kb on + strand, within HMPREF1078_RS05910 at 516.011 kb on - strand at 516.138 kb on + strand at 516.138 kb on + strand at 516.138 kb on + strand at 516.231 kb on + strand at 516.235 kb on + strand at 516.235 kb on + strand at 516.236 kb on - strand at 516.236 kb on - strand at 516.236 kb on - strand at 516.236 kb on - strand at 516.259 kb on - strand at 516.261 kb on + strand at 516.386 kb on - strand at 516.549 kb on - strand at 516.563 kb on - strand at 516.765 kb on - strand, within HMPREF1078_RS05915 at 516.787 kb on + strand, within HMPREF1078_RS05915 at 516.787 kb on + strand, within HMPREF1078_RS05915 at 516.788 kb on - strand, within HMPREF1078_RS05915 at 516.788 kb on - strand, within HMPREF1078_RS05915 at 516.788 kb on - strand, within HMPREF1078_RS05915 at 516.855 kb on - strand, within HMPREF1078_RS05915 at 516.857 kb on - strand, within HMPREF1078_RS05915 at 516.949 kb on + strand, within HMPREF1078_RS05915 at 517.130 kb on - strand, within HMPREF1078_RS05915 at 517.132 kb on + strand, within HMPREF1078_RS05915 at 517.176 kb on + strand, within HMPREF1078_RS05915 at 517.177 kb on - strand, within HMPREF1078_RS05915 at 517.262 kb on + strand, within HMPREF1078_RS05915 at 517.263 kb on - strand, within HMPREF1078_RS05915 at 517.313 kb on + strand, within HMPREF1078_RS05915 at 517.313 kb on + strand, within HMPREF1078_RS05915 at 517.332 kb on + strand, within HMPREF1078_RS05915 at 517.333 kb on - strand, within HMPREF1078_RS05915 at 517.333 kb on - strand, within HMPREF1078_RS05915 at 517.336 kb on + strand, within HMPREF1078_RS05915 at 517.337 kb on - strand, within HMPREF1078_RS05915 at 517.350 kb on + strand, within HMPREF1078_RS05915 at 517.350 kb on + strand, within HMPREF1078_RS05915 at 517.367 kb on - strand, within HMPREF1078_RS05915 at 517.466 kb on + strand, within HMPREF1078_RS05915 at 517.593 kb on + strand, within HMPREF1078_RS05915 at 517.593 kb on + strand, within HMPREF1078_RS05915 at 517.613 kb on - strand, within HMPREF1078_RS05915 at 517.622 kb on - strand, within HMPREF1078_RS05915 at 517.623 kb on + strand, within HMPREF1078_RS05915 at 517.821 kb on + strand, within HMPREF1078_RS05915 at 517.822 kb on - strand, within HMPREF1078_RS05915 at 517.823 kb on + strand, within HMPREF1078_RS05915 at 517.823 kb on + strand, within HMPREF1078_RS05915 at 517.847 kb on - strand, within HMPREF1078_RS05915 at 517.912 kb on - strand, within HMPREF1078_RS05915 at 517.938 kb on - strand, within HMPREF1078_RS05915 at 517.961 kb on - strand, within HMPREF1078_RS05915 at 517.961 kb on - strand, within HMPREF1078_RS05915 at 517.961 kb on - strand, within HMPREF1078_RS05915 at 517.961 kb on - strand, within HMPREF1078_RS05915 at 517.961 kb on - strand, within HMPREF1078_RS05915 at 518.039 kb on - strand, within HMPREF1078_RS05915 at 518.039 kb on - strand, within HMPREF1078_RS05915 at 518.050 kb on + strand, within HMPREF1078_RS05915 at 518.176 kb on + strand, within HMPREF1078_RS05915 at 518.399 kb on - strand at 518.429 kb on - strand at 518.430 kb on + strand at 518.478 kb on - strand at 518.478 kb on - strand at 518.480 kb on + strand at 518.527 kb on + strand at 518.527 kb on + strand at 518.528 kb on - strand at 518.528 kb on - strand at 518.528 kb on - strand at 518.528 kb on - strand at 518.528 kb on - strand at 518.529 kb on + strand at 518.591 kb on + strand at 518.603 kb on - strand at 518.607 kb on + strand at 518.607 kb on + strand at 518.724 kb on + strand at 518.999 kb on + strand, within HMPREF1078_RS05920 at 518.999 kb on + strand, within HMPREF1078_RS05920 at 519.145 kb on + strand, within HMPREF1078_RS05920 at 519.419 kb on - strand, within HMPREF1078_RS05920
Per-strain Table
Position Strand Gene LocusTag Fraction Pectin from citrus peel; autoclaved 2.5 mg/mL (C) remove 515,455 + HMPREF1078_RS05910 0.62 -2.3 515,486 + HMPREF1078_RS05910 0.64 -1.6 515,491 + HMPREF1078_RS05910 0.64 -1.4 515,621 + HMPREF1078_RS05910 0.72 -0.7 515,622 - HMPREF1078_RS05910 0.72 -1.9 515,674 + HMPREF1078_RS05910 0.75 -1.1 515,676 + HMPREF1078_RS05910 0.75 -3.0 515,689 + HMPREF1078_RS05910 0.76 -1.5 515,689 + HMPREF1078_RS05910 0.76 -2.5 515,727 + HMPREF1078_RS05910 0.78 -1.5 515,728 - HMPREF1078_RS05910 0.78 -0.5 515,728 - HMPREF1078_RS05910 0.78 -2.3 515,903 + HMPREF1078_RS05910 0.89 -0.9 516,011 - -2.1 516,138 + +1.0 516,138 + +0.1 516,138 + +3.2 516,231 + +0.5 516,235 + -1.7 516,235 + -0.5 516,236 - +0.7 516,236 - +1.0 516,236 - +1.4 516,236 - +0.5 516,259 - +0.2 516,261 + +0.1 516,386 - +1.1 516,549 - +0.5 516,563 - +1.1 516,765 - HMPREF1078_RS05915 0.18 +0.3 516,787 + HMPREF1078_RS05915 0.20 -0.9 516,787 + HMPREF1078_RS05915 0.20 -0.7 516,788 - HMPREF1078_RS05915 0.20 +0.3 516,788 - HMPREF1078_RS05915 0.20 +1.3 516,788 - HMPREF1078_RS05915 0.20 +0.7 516,855 - HMPREF1078_RS05915 0.23 -0.3 516,857 - HMPREF1078_RS05915 0.23 +0.7 516,949 + HMPREF1078_RS05915 0.27 +2.7 517,130 - HMPREF1078_RS05915 0.35 -0.1 517,132 + HMPREF1078_RS05915 0.35 -1.3 517,176 + HMPREF1078_RS05915 0.37 +0.9 517,177 - HMPREF1078_RS05915 0.38 -0.5 517,262 + HMPREF1078_RS05915 0.41 -0.9 517,263 - HMPREF1078_RS05915 0.42 -0.4 517,313 + HMPREF1078_RS05915 0.44 -0.2 517,313 + HMPREF1078_RS05915 0.44 -2.4 517,332 + HMPREF1078_RS05915 0.45 -2.6 517,333 - HMPREF1078_RS05915 0.45 -2.8 517,333 - HMPREF1078_RS05915 0.45 -3.3 517,336 + HMPREF1078_RS05915 0.45 +0.1 517,337 - HMPREF1078_RS05915 0.45 -0.5 517,350 + HMPREF1078_RS05915 0.46 +0.9 517,350 + HMPREF1078_RS05915 0.46 -1.5 517,367 - HMPREF1078_RS05915 0.46 +0.2 517,466 + HMPREF1078_RS05915 0.51 -0.1 517,593 + HMPREF1078_RS05915 0.57 +1.2 517,593 + HMPREF1078_RS05915 0.57 +0.1 517,613 - HMPREF1078_RS05915 0.58 +0.8 517,622 - HMPREF1078_RS05915 0.58 -1.3 517,623 + HMPREF1078_RS05915 0.58 +0.7 517,821 + HMPREF1078_RS05915 0.67 +1.2 517,822 - HMPREF1078_RS05915 0.67 +0.6 517,823 + HMPREF1078_RS05915 0.67 -0.5 517,823 + HMPREF1078_RS05915 0.67 +0.7 517,847 - HMPREF1078_RS05915 0.69 -0.9 517,912 - HMPREF1078_RS05915 0.72 -1.0 517,938 - HMPREF1078_RS05915 0.73 +1.2 517,961 - HMPREF1078_RS05915 0.74 +1.4 517,961 - HMPREF1078_RS05915 0.74 +1.5 517,961 - HMPREF1078_RS05915 0.74 +0.2 517,961 - HMPREF1078_RS05915 0.74 -1.1 517,961 - HMPREF1078_RS05915 0.74 +1.0 518,039 - HMPREF1078_RS05915 0.77 -1.1 518,039 - HMPREF1078_RS05915 0.77 -1.2 518,050 + HMPREF1078_RS05915 0.78 -0.3 518,176 + HMPREF1078_RS05915 0.84 +0.7 518,399 - -0.5 518,429 - -0.9 518,430 + +1.0 518,478 - -0.1 518,478 - -2.2 518,480 + +0.0 518,527 + +0.4 518,527 + -0.2 518,528 - -1.8 518,528 - -0.6 518,528 - -2.2 518,528 - -2.2 518,528 - +0.4 518,529 + +1.7 518,591 + +1.8 518,603 - +0.4 518,607 + -1.4 518,607 + -2.6 518,724 + -2.8 518,999 + HMPREF1078_RS05920 0.19 +0.5 518,999 + HMPREF1078_RS05920 0.19 -2.1 519,145 + HMPREF1078_RS05920 0.29 +2.9 519,419 - HMPREF1078_RS05920 0.47 -0.6
Or see this region's nucleotide sequence