Experiment: Pectin from citrus peel; autoclaved 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS03485 and HMPREF1078_RS03490 are separated by 139 nucleotides HMPREF1078_RS03490 and HMPREF1078_RS03495 are separated by 170 nucleotides HMPREF1078_RS03495 and HMPREF1078_RS03500 are separated by 112 nucleotides
HMPREF1078_RS03485: HMPREF1078_RS03485 - phosphomethylpyrimidine synthase ThiC, at 822,218 to 823,924
_RS03485
HMPREF1078_RS03490: HMPREF1078_RS03490 - response regulator transcription factor, at 824,064 to 824,963
_RS03490
HMPREF1078_RS03495: HMPREF1078_RS03495 - hypothetical protein, at 825,134 to 825,637
_RS03495
HMPREF1078_RS03500: HMPREF1078_RS03500 - carboxymuconolactone decarboxylase family protein, at 825,750 to 827,425
_RS03500
Position (kb)
824
825 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 823.201 kb on + strand, within HMPREF1078_RS03485 at 823.201 kb on + strand, within HMPREF1078_RS03485 at 823.257 kb on + strand, within HMPREF1078_RS03485 at 823.258 kb on - strand, within HMPREF1078_RS03485 at 823.277 kb on + strand, within HMPREF1078_RS03485 at 823.278 kb on - strand, within HMPREF1078_RS03485 at 823.325 kb on + strand, within HMPREF1078_RS03485 at 823.325 kb on + strand, within HMPREF1078_RS03485 at 823.326 kb on - strand, within HMPREF1078_RS03485 at 823.326 kb on - strand, within HMPREF1078_RS03485 at 823.363 kb on + strand, within HMPREF1078_RS03485 at 823.363 kb on + strand, within HMPREF1078_RS03485 at 823.444 kb on + strand, within HMPREF1078_RS03485 at 823.445 kb on - strand, within HMPREF1078_RS03485 at 823.475 kb on + strand, within HMPREF1078_RS03485 at 823.476 kb on - strand, within HMPREF1078_RS03485 at 823.476 kb on - strand, within HMPREF1078_RS03485 at 823.526 kb on - strand, within HMPREF1078_RS03485 at 823.526 kb on - strand, within HMPREF1078_RS03485 at 823.539 kb on - strand, within HMPREF1078_RS03485 at 823.605 kb on - strand, within HMPREF1078_RS03485 at 823.605 kb on - strand, within HMPREF1078_RS03485 at 823.818 kb on - strand at 823.847 kb on + strand at 823.899 kb on + strand at 823.900 kb on - strand at 823.903 kb on + strand at 823.920 kb on + strand at 823.921 kb on - strand at 823.921 kb on - strand at 823.923 kb on - strand at 823.923 kb on - strand at 823.954 kb on + strand at 823.954 kb on + strand at 823.954 kb on + strand at 823.954 kb on + strand at 823.955 kb on - strand at 823.955 kb on - strand at 823.955 kb on - strand at 823.956 kb on + strand at 823.957 kb on - strand at 823.957 kb on - strand at 823.958 kb on + strand at 823.959 kb on - strand at 823.973 kb on - strand at 823.974 kb on + strand at 823.974 kb on + strand at 823.974 kb on + strand at 823.975 kb on - strand at 823.989 kb on - strand at 824.156 kb on - strand, within HMPREF1078_RS03490 at 824.226 kb on + strand, within HMPREF1078_RS03490 at 824.230 kb on + strand, within HMPREF1078_RS03490 at 824.230 kb on + strand, within HMPREF1078_RS03490 at 824.231 kb on - strand, within HMPREF1078_RS03490 at 824.231 kb on - strand, within HMPREF1078_RS03490 at 824.231 kb on - strand, within HMPREF1078_RS03490 at 824.317 kb on - strand, within HMPREF1078_RS03490 at 824.360 kb on - strand, within HMPREF1078_RS03490 at 824.428 kb on - strand, within HMPREF1078_RS03490 at 824.434 kb on + strand, within HMPREF1078_RS03490 at 824.435 kb on - strand, within HMPREF1078_RS03490 at 824.445 kb on - strand, within HMPREF1078_RS03490 at 824.449 kb on + strand, within HMPREF1078_RS03490 at 824.492 kb on + strand, within HMPREF1078_RS03490 at 824.493 kb on - strand, within HMPREF1078_RS03490 at 824.493 kb on - strand, within HMPREF1078_RS03490 at 824.493 kb on - strand, within HMPREF1078_RS03490 at 824.493 kb on - strand, within HMPREF1078_RS03490 at 824.493 kb on - strand, within HMPREF1078_RS03490 at 824.611 kb on + strand, within HMPREF1078_RS03490 at 824.612 kb on - strand, within HMPREF1078_RS03490 at 824.646 kb on + strand, within HMPREF1078_RS03490 at 824.647 kb on - strand, within HMPREF1078_RS03490 at 824.702 kb on + strand, within HMPREF1078_RS03490 at 824.702 kb on + strand, within HMPREF1078_RS03490 at 824.780 kb on - strand, within HMPREF1078_RS03490 at 824.780 kb on - strand, within HMPREF1078_RS03490 at 824.785 kb on - strand, within HMPREF1078_RS03490 at 824.802 kb on + strand, within HMPREF1078_RS03490 at 824.802 kb on + strand, within HMPREF1078_RS03490 at 824.802 kb on + strand, within HMPREF1078_RS03490 at 824.803 kb on - strand, within HMPREF1078_RS03490 at 824.804 kb on + strand, within HMPREF1078_RS03490 at 824.805 kb on - strand, within HMPREF1078_RS03490 at 824.805 kb on - strand, within HMPREF1078_RS03490 at 824.815 kb on + strand, within HMPREF1078_RS03490 at 824.824 kb on + strand, within HMPREF1078_RS03490 at 824.824 kb on + strand, within HMPREF1078_RS03490 at 824.903 kb on + strand at 824.907 kb on + strand at 824.907 kb on + strand at 824.907 kb on + strand at 824.907 kb on + strand at 824.907 kb on + strand at 824.907 kb on + strand at 824.907 kb on + strand at 824.908 kb on - strand at 824.908 kb on - strand at 824.908 kb on - strand at 824.924 kb on + strand at 824.925 kb on - strand at 824.950 kb on - strand at 824.952 kb on - strand at 824.971 kb on + strand at 825.034 kb on + strand at 825.035 kb on - strand at 825.039 kb on - strand at 825.060 kb on - strand at 825.060 kb on - strand at 825.084 kb on + strand at 825.121 kb on - strand at 825.121 kb on - strand at 825.139 kb on + strand at 825.151 kb on + strand at 825.151 kb on + strand at 825.152 kb on - strand at 825.152 kb on - strand at 825.152 kb on - strand at 825.152 kb on - strand at 825.382 kb on + strand, within HMPREF1078_RS03495 at 825.546 kb on + strand, within HMPREF1078_RS03495 at 825.568 kb on + strand, within HMPREF1078_RS03495 at 825.593 kb on - strand at 825.603 kb on + strand at 825.604 kb on - strand at 825.616 kb on + strand at 825.617 kb on - strand at 825.617 kb on - strand at 825.619 kb on - strand at 825.637 kb on + strand at 825.640 kb on - strand at 825.658 kb on - strand at 825.734 kb on + strand at 825.739 kb on + strand at 825.750 kb on - strand at 825.781 kb on + strand at 825.838 kb on + strand at 825.842 kb on + strand at 825.851 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Pectin from citrus peel; autoclaved 2.5 mg/mL (C) remove 823,201 + HMPREF1078_RS03485 0.58 -1.0 823,201 + HMPREF1078_RS03485 0.58 +1.2 823,257 + HMPREF1078_RS03485 0.61 -1.6 823,258 - HMPREF1078_RS03485 0.61 -0.5 823,277 + HMPREF1078_RS03485 0.62 +0.0 823,278 - HMPREF1078_RS03485 0.62 -2.4 823,325 + HMPREF1078_RS03485 0.65 -0.5 823,325 + HMPREF1078_RS03485 0.65 +0.8 823,326 - HMPREF1078_RS03485 0.65 +2.0 823,326 - HMPREF1078_RS03485 0.65 -1.0 823,363 + HMPREF1078_RS03485 0.67 -0.7 823,363 + HMPREF1078_RS03485 0.67 +0.9 823,444 + HMPREF1078_RS03485 0.72 +0.6 823,445 - HMPREF1078_RS03485 0.72 -0.3 823,475 + HMPREF1078_RS03485 0.74 -1.3 823,476 - HMPREF1078_RS03485 0.74 -0.1 823,476 - HMPREF1078_RS03485 0.74 +1.3 823,526 - HMPREF1078_RS03485 0.77 +0.1 823,526 - HMPREF1078_RS03485 0.77 -0.1 823,539 - HMPREF1078_RS03485 0.77 -0.7 823,605 - HMPREF1078_RS03485 0.81 +0.5 823,605 - HMPREF1078_RS03485 0.81 -1.4 823,818 - -1.2 823,847 + -0.1 823,899 + -0.2 823,900 - +0.9 823,903 + -0.3 823,920 + -0.2 823,921 - +1.6 823,921 - +0.5 823,923 - -1.7 823,923 - -1.0 823,954 + +0.6 823,954 + +1.2 823,954 + -0.3 823,954 + +0.8 823,955 - +0.1 823,955 - -2.2 823,955 - +0.5 823,956 + -0.6 823,957 - +0.3 823,957 - -0.5 823,958 + +1.0 823,959 - -2.6 823,973 - -1.6 823,974 + -1.8 823,974 + +0.3 823,974 + +0.9 823,975 - -1.1 823,989 - -0.0 824,156 - HMPREF1078_RS03490 0.10 +0.6 824,226 + HMPREF1078_RS03490 0.18 +0.7 824,230 + HMPREF1078_RS03490 0.18 +0.2 824,230 + HMPREF1078_RS03490 0.18 -2.6 824,231 - HMPREF1078_RS03490 0.19 +0.1 824,231 - HMPREF1078_RS03490 0.19 -1.4 824,231 - HMPREF1078_RS03490 0.19 +0.4 824,317 - HMPREF1078_RS03490 0.28 -2.7 824,360 - HMPREF1078_RS03490 0.33 +1.8 824,428 - HMPREF1078_RS03490 0.40 -0.1 824,434 + HMPREF1078_RS03490 0.41 -2.3 824,435 - HMPREF1078_RS03490 0.41 -1.7 824,445 - HMPREF1078_RS03490 0.42 -1.9 824,449 + HMPREF1078_RS03490 0.43 +0.3 824,492 + HMPREF1078_RS03490 0.48 -0.4 824,493 - HMPREF1078_RS03490 0.48 -1.5 824,493 - HMPREF1078_RS03490 0.48 +0.1 824,493 - HMPREF1078_RS03490 0.48 -0.3 824,493 - HMPREF1078_RS03490 0.48 +1.3 824,493 - HMPREF1078_RS03490 0.48 +0.3 824,611 + HMPREF1078_RS03490 0.61 +0.9 824,612 - HMPREF1078_RS03490 0.61 -0.3 824,646 + HMPREF1078_RS03490 0.65 +0.5 824,647 - HMPREF1078_RS03490 0.65 -3.7 824,702 + HMPREF1078_RS03490 0.71 -0.8 824,702 + HMPREF1078_RS03490 0.71 -0.8 824,780 - HMPREF1078_RS03490 0.80 -1.2 824,780 - HMPREF1078_RS03490 0.80 -0.3 824,785 - HMPREF1078_RS03490 0.80 -0.1 824,802 + HMPREF1078_RS03490 0.82 -0.5 824,802 + HMPREF1078_RS03490 0.82 -2.9 824,802 + HMPREF1078_RS03490 0.82 -2.4 824,803 - HMPREF1078_RS03490 0.82 +0.6 824,804 + HMPREF1078_RS03490 0.82 -0.3 824,805 - HMPREF1078_RS03490 0.82 -0.5 824,805 - HMPREF1078_RS03490 0.82 +0.7 824,815 + HMPREF1078_RS03490 0.83 -1.1 824,824 + HMPREF1078_RS03490 0.84 -2.8 824,824 + HMPREF1078_RS03490 0.84 -0.1 824,903 + -0.6 824,907 + -0.1 824,907 + -2.7 824,907 + -1.3 824,907 + -0.4 824,907 + -1.2 824,907 + +0.1 824,907 + -0.7 824,908 - -0.7 824,908 - -0.1 824,908 - -0.7 824,924 + +0.5 824,925 - -2.6 824,950 - +1.0 824,952 - +1.8 824,971 + -2.1 825,034 + +1.8 825,035 - +2.5 825,039 - +1.9 825,060 - -0.7 825,060 - +0.3 825,084 + -0.7 825,121 - +0.6 825,121 - -0.1 825,139 + -0.8 825,151 + +1.0 825,151 + +0.1 825,152 - -0.7 825,152 - -0.5 825,152 - -1.9 825,152 - -1.8 825,382 + HMPREF1078_RS03495 0.49 +0.9 825,546 + HMPREF1078_RS03495 0.82 +1.5 825,568 + HMPREF1078_RS03495 0.86 -2.1 825,593 - -0.5 825,603 + -2.0 825,604 - -0.8 825,616 + -2.9 825,617 - -1.6 825,617 - +2.1 825,619 - -1.2 825,637 + -0.9 825,640 - -1.1 825,658 - +0.2 825,734 + -1.1 825,739 + -1.7 825,750 - +0.7 825,781 + +0.0 825,838 + -1.9 825,842 + -1.8 825,851 + -2.9
Or see this region's nucleotide sequence