Experiment: .-Glucan from yeast 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS08155 and HMPREF1078_RS08160 overlap by 47 nucleotides HMPREF1078_RS08160 and HMPREF1078_RS19865 overlap by 65 nucleotides HMPREF1078_RS19865 and HMPREF1078_RS08165 are separated by 118 nucleotides HMPREF1078_RS08165 and HMPREF1078_RS19560 are separated by 177 nucleotides HMPREF1078_RS19560 and HMPREF1078_RS18645 are separated by 128 nucleotides HMPREF1078_RS18645 and HMPREF1078_RS08170 are separated by 210 nucleotides
HMPREF1078_RS08155: HMPREF1078_RS08155 - IS5 family transposase, at 200,801 to 201,265
_RS08155
HMPREF1078_RS08160: HMPREF1078_RS08160 - transposase, at 201,219 to 201,506
_RS08160
HMPREF1078_RS19865: HMPREF1078_RS19865 - hypothetical protein, at 201,442 to 201,696
_RS19865
HMPREF1078_RS08165: HMPREF1078_RS08165 - helix-turn-helix domain-containing protein, at 201,815 to 202,027
_RS08165
HMPREF1078_RS19560: HMPREF1078_RS19560 - Ppx/GppA family phosphatase, at 202,205 to 202,381
_RS19560
HMPREF1078_RS18645: HMPREF1078_RS18645 - transposase, at 202,510 to 202,638
_RS18645
HMPREF1078_RS08170: HMPREF1078_RS08170 - methylglyoxal synthase, at 202,849 to 203,385
_RS08170
Position (kb) 202
203 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 201.215 kb on + strand, within HMPREF1078_RS08155 at 201.221 kb on - strand at 201.241 kb on + strand at 201.242 kb on - strand at 201.242 kb on - strand at 201.242 kb on - strand at 201.281 kb on + strand, within HMPREF1078_RS08160 at 201.281 kb on + strand, within HMPREF1078_RS08160 at 201.282 kb on - strand, within HMPREF1078_RS08160 at 201.334 kb on - strand, within HMPREF1078_RS08160 at 201.351 kb on - strand, within HMPREF1078_RS08160 at 201.384 kb on - strand, within HMPREF1078_RS08160 at 201.420 kb on - strand, within HMPREF1078_RS08160 at 201.453 kb on - strand at 201.453 kb on - strand at 201.489 kb on + strand at 201.490 kb on - strand at 201.490 kb on - strand at 201.490 kb on - strand at 201.490 kb on - strand at 201.495 kb on + strand at 201.536 kb on + strand, within HMPREF1078_RS19865 at 201.537 kb on - strand, within HMPREF1078_RS19865 at 201.537 kb on - strand, within HMPREF1078_RS19865 at 201.541 kb on + strand, within HMPREF1078_RS19865 at 201.542 kb on - strand, within HMPREF1078_RS19865 at 201.542 kb on - strand, within HMPREF1078_RS19865 at 201.543 kb on + strand, within HMPREF1078_RS19865 at 201.543 kb on + strand, within HMPREF1078_RS19865 at 201.544 kb on - strand, within HMPREF1078_RS19865 at 201.544 kb on - strand, within HMPREF1078_RS19865 at 201.558 kb on + strand, within HMPREF1078_RS19865 at 201.558 kb on + strand, within HMPREF1078_RS19865 at 201.571 kb on - strand, within HMPREF1078_RS19865 at 201.581 kb on + strand, within HMPREF1078_RS19865 at 201.584 kb on + strand, within HMPREF1078_RS19865 at 201.585 kb on - strand, within HMPREF1078_RS19865 at 201.585 kb on - strand, within HMPREF1078_RS19865 at 201.610 kb on + strand, within HMPREF1078_RS19865 at 201.610 kb on + strand, within HMPREF1078_RS19865 at 201.641 kb on - strand, within HMPREF1078_RS19865 at 201.689 kb on + strand at 201.690 kb on - strand at 201.710 kb on + strand at 201.769 kb on + strand at 201.769 kb on + strand at 201.770 kb on - strand at 201.770 kb on - strand at 201.770 kb on - strand at 201.773 kb on + strand at 201.773 kb on + strand at 201.773 kb on + strand at 201.774 kb on - strand at 201.774 kb on - strand at 201.898 kb on - strand, within HMPREF1078_RS08165 at 201.901 kb on - strand, within HMPREF1078_RS08165 at 201.901 kb on - strand, within HMPREF1078_RS08165 at 201.904 kb on + strand, within HMPREF1078_RS08165 at 201.904 kb on + strand, within HMPREF1078_RS08165 at 201.905 kb on - strand, within HMPREF1078_RS08165 at 201.905 kb on - strand, within HMPREF1078_RS08165 at 202.062 kb on + strand at 202.066 kb on + strand at 202.067 kb on - strand at 202.154 kb on - strand at 202.202 kb on + strand at 202.203 kb on - strand at 202.222 kb on + strand at 202.249 kb on + strand, within HMPREF1078_RS19560 at 202.249 kb on + strand, within HMPREF1078_RS19560 at 202.249 kb on + strand, within HMPREF1078_RS19560 at 202.250 kb on - strand, within HMPREF1078_RS19560 at 202.278 kb on + strand, within HMPREF1078_RS19560 at 202.289 kb on - strand, within HMPREF1078_RS19560 at 202.304 kb on + strand, within HMPREF1078_RS19560 at 202.305 kb on - strand, within HMPREF1078_RS19560 at 202.306 kb on + strand, within HMPREF1078_RS19560 at 202.330 kb on - strand, within HMPREF1078_RS19560 at 202.330 kb on - strand, within HMPREF1078_RS19560 at 202.340 kb on - strand, within HMPREF1078_RS19560 at 202.362 kb on + strand, within HMPREF1078_RS19560 at 202.362 kb on + strand, within HMPREF1078_RS19560 at 202.363 kb on - strand, within HMPREF1078_RS19560 at 202.363 kb on - strand, within HMPREF1078_RS19560 at 202.363 kb on - strand, within HMPREF1078_RS19560 at 202.364 kb on + strand at 202.405 kb on - strand at 202.409 kb on - strand at 202.409 kb on - strand at 202.422 kb on + strand at 202.422 kb on + strand at 202.445 kb on + strand at 202.456 kb on - strand at 202.485 kb on + strand at 202.491 kb on + strand at 202.491 kb on + strand at 202.624 kb on - strand, within HMPREF1078_RS18645 at 202.630 kb on + strand at 202.631 kb on - strand at 202.631 kb on - strand at 202.637 kb on - strand at 202.716 kb on - strand at 202.722 kb on + strand at 202.723 kb on - strand at 202.757 kb on - strand at 202.765 kb on - strand at 202.778 kb on + strand at 202.778 kb on + strand at 202.779 kb on - strand at 202.782 kb on + strand at 202.782 kb on + strand at 202.782 kb on + strand at 202.783 kb on - strand at 202.783 kb on - strand at 202.783 kb on - strand at 202.796 kb on + strand at 202.796 kb on + strand at 202.812 kb on + strand at 202.812 kb on + strand at 202.813 kb on - strand at 202.822 kb on + strand at 202.823 kb on - strand at 202.823 kb on - strand at 202.824 kb on + strand at 202.824 kb on + strand at 202.828 kb on + strand at 202.828 kb on + strand at 202.828 kb on + strand at 202.828 kb on + strand at 202.837 kb on - strand at 202.837 kb on - strand at 202.843 kb on + strand at 202.843 kb on + strand at 202.843 kb on + strand at 202.864 kb on - strand at 202.912 kb on + strand, within HMPREF1078_RS08170 at 202.943 kb on + strand, within HMPREF1078_RS08170 at 202.943 kb on + strand, within HMPREF1078_RS08170 at 202.965 kb on + strand, within HMPREF1078_RS08170 at 202.973 kb on + strand, within HMPREF1078_RS08170 at 203.002 kb on - strand, within HMPREF1078_RS08170 at 203.004 kb on + strand, within HMPREF1078_RS08170 at 203.005 kb on - strand, within HMPREF1078_RS08170 at 203.044 kb on - strand, within HMPREF1078_RS08170 at 203.098 kb on + strand, within HMPREF1078_RS08170 at 203.116 kb on - strand, within HMPREF1078_RS08170 at 203.130 kb on - strand, within HMPREF1078_RS08170 at 203.130 kb on - strand, within HMPREF1078_RS08170 at 203.147 kb on - strand, within HMPREF1078_RS08170 at 203.252 kb on - strand, within HMPREF1078_RS08170 at 203.266 kb on - strand, within HMPREF1078_RS08170 at 203.271 kb on + strand, within HMPREF1078_RS08170 at 203.271 kb on + strand, within HMPREF1078_RS08170 at 203.272 kb on - strand, within HMPREF1078_RS08170 at 203.277 kb on - strand, within HMPREF1078_RS08170 at 203.290 kb on + strand, within HMPREF1078_RS08170 at 203.323 kb on + strand, within HMPREF1078_RS08170 at 203.323 kb on + strand, within HMPREF1078_RS08170 at 203.324 kb on - strand, within HMPREF1078_RS08170 at 203.324 kb on - strand, within HMPREF1078_RS08170 at 203.324 kb on - strand, within HMPREF1078_RS08170 at 203.324 kb on - strand, within HMPREF1078_RS08170
Per-strain Table
Position Strand Gene LocusTag Fraction .-Glucan from yeast 2.5 mg/mL (C) remove 201,215 + HMPREF1078_RS08155 0.89 +0.8 201,221 - -2.5 201,241 + -0.9 201,242 - +0.6 201,242 - -0.0 201,242 - -1.5 201,281 + HMPREF1078_RS08160 0.22 +1.6 201,281 + HMPREF1078_RS08160 0.22 -0.1 201,282 - HMPREF1078_RS08160 0.22 -2.5 201,334 - HMPREF1078_RS08160 0.40 -0.9 201,351 - HMPREF1078_RS08160 0.46 -2.5 201,384 - HMPREF1078_RS08160 0.57 -2.5 201,420 - HMPREF1078_RS08160 0.70 +2.4 201,453 - -0.8 201,453 - +1.1 201,489 + -0.3 201,490 - +0.9 201,490 - -2.4 201,490 - +1.0 201,490 - -0.4 201,495 + -1.6 201,536 + HMPREF1078_RS19865 0.37 -0.7 201,537 - HMPREF1078_RS19865 0.37 +1.2 201,537 - HMPREF1078_RS19865 0.37 +0.8 201,541 + HMPREF1078_RS19865 0.39 -1.7 201,542 - HMPREF1078_RS19865 0.39 +0.4 201,542 - HMPREF1078_RS19865 0.39 +2.7 201,543 + HMPREF1078_RS19865 0.40 -2.4 201,543 + HMPREF1078_RS19865 0.40 +2.2 201,544 - HMPREF1078_RS19865 0.40 +2.0 201,544 - HMPREF1078_RS19865 0.40 +1.8 201,558 + HMPREF1078_RS19865 0.45 +1.5 201,558 + HMPREF1078_RS19865 0.45 -0.2 201,571 - HMPREF1078_RS19865 0.51 +0.2 201,581 + HMPREF1078_RS19865 0.55 -1.5 201,584 + HMPREF1078_RS19865 0.56 +0.8 201,585 - HMPREF1078_RS19865 0.56 -3.5 201,585 - HMPREF1078_RS19865 0.56 -1.9 201,610 + HMPREF1078_RS19865 0.66 -1.2 201,610 + HMPREF1078_RS19865 0.66 +1.7 201,641 - HMPREF1078_RS19865 0.78 -1.5 201,689 + +1.9 201,690 - -1.5 201,710 + +0.6 201,769 + +0.8 201,769 + -1.9 201,770 - -2.2 201,770 - -0.8 201,770 - +0.1 201,773 + -1.9 201,773 + +1.6 201,773 + +1.4 201,774 - +0.8 201,774 - +0.6 201,898 - HMPREF1078_RS08165 0.39 -1.2 201,901 - HMPREF1078_RS08165 0.40 -1.9 201,901 - HMPREF1078_RS08165 0.40 -0.5 201,904 + HMPREF1078_RS08165 0.42 -0.9 201,904 + HMPREF1078_RS08165 0.42 -1.9 201,905 - HMPREF1078_RS08165 0.42 -1.0 201,905 - HMPREF1078_RS08165 0.42 -1.2 202,062 + +0.3 202,066 + +1.8 202,067 - +0.8 202,154 - +0.3 202,202 + -0.4 202,203 - -1.9 202,222 + -2.6 202,249 + HMPREF1078_RS19560 0.25 +0.1 202,249 + HMPREF1078_RS19560 0.25 +0.7 202,249 + HMPREF1078_RS19560 0.25 +1.2 202,250 - HMPREF1078_RS19560 0.25 +0.5 202,278 + HMPREF1078_RS19560 0.41 -0.6 202,289 - HMPREF1078_RS19560 0.47 +1.9 202,304 + HMPREF1078_RS19560 0.56 -1.9 202,305 - HMPREF1078_RS19560 0.56 +1.6 202,306 + HMPREF1078_RS19560 0.57 +2.0 202,330 - HMPREF1078_RS19560 0.71 +0.9 202,330 - HMPREF1078_RS19560 0.71 -0.9 202,340 - HMPREF1078_RS19560 0.76 -0.7 202,362 + HMPREF1078_RS19560 0.89 -1.8 202,362 + HMPREF1078_RS19560 0.89 -1.2 202,363 - HMPREF1078_RS19560 0.89 +1.5 202,363 - HMPREF1078_RS19560 0.89 +1.3 202,363 - HMPREF1078_RS19560 0.89 +0.2 202,364 + -2.1 202,405 - -1.2 202,409 - -2.3 202,409 - -2.2 202,422 + +1.2 202,422 + -1.2 202,445 + +0.9 202,456 - -1.7 202,485 + -1.4 202,491 + +0.8 202,491 + -2.1 202,624 - HMPREF1078_RS18645 0.88 -2.6 202,630 + -1.1 202,631 - -2.4 202,631 - +0.7 202,637 - -1.9 202,716 - +0.3 202,722 + -2.4 202,723 - -0.9 202,757 - +1.2 202,765 - -0.7 202,778 + -2.0 202,778 + +0.3 202,779 - -0.5 202,782 + +0.4 202,782 + -3.0 202,782 + +0.5 202,783 - -1.9 202,783 - +2.2 202,783 - +2.4 202,796 + +2.2 202,796 + +0.8 202,812 + +0.6 202,812 + -0.8 202,813 - +0.5 202,822 + +0.6 202,823 - -0.3 202,823 - +1.5 202,824 + +0.8 202,824 + +0.4 202,828 + -3.4 202,828 + +0.3 202,828 + -0.2 202,828 + +0.2 202,837 - -0.4 202,837 - -3.0 202,843 + +2.2 202,843 + -0.4 202,843 + +1.0 202,864 - -2.3 202,912 + HMPREF1078_RS08170 0.12 -1.5 202,943 + HMPREF1078_RS08170 0.18 +0.7 202,943 + HMPREF1078_RS08170 0.18 -3.4 202,965 + HMPREF1078_RS08170 0.22 -2.5 202,973 + HMPREF1078_RS08170 0.23 -0.7 203,002 - HMPREF1078_RS08170 0.28 -1.5 203,004 + HMPREF1078_RS08170 0.29 -0.4 203,005 - HMPREF1078_RS08170 0.29 +1.3 203,044 - HMPREF1078_RS08170 0.36 +1.2 203,098 + HMPREF1078_RS08170 0.46 +0.9 203,116 - HMPREF1078_RS08170 0.50 -0.3 203,130 - HMPREF1078_RS08170 0.52 +0.1 203,130 - HMPREF1078_RS08170 0.52 -1.3 203,147 - HMPREF1078_RS08170 0.55 -0.9 203,252 - HMPREF1078_RS08170 0.75 -1.7 203,266 - HMPREF1078_RS08170 0.78 +0.5 203,271 + HMPREF1078_RS08170 0.79 -0.2 203,271 + HMPREF1078_RS08170 0.79 -0.5 203,272 - HMPREF1078_RS08170 0.79 -0.2 203,277 - HMPREF1078_RS08170 0.80 +1.1 203,290 + HMPREF1078_RS08170 0.82 -1.0 203,323 + HMPREF1078_RS08170 0.88 -1.0 203,323 + HMPREF1078_RS08170 0.88 -0.8 203,324 - HMPREF1078_RS08170 0.88 -1.2 203,324 - HMPREF1078_RS08170 0.88 -2.0 203,324 - HMPREF1078_RS08170 0.88 -2.3 203,324 - HMPREF1078_RS08170 0.88 -2.5
Or see this region's nucleotide sequence