Experiment: .-Glucan from yeast 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS00315 and HMPREF1078_RS00320 are separated by 84 nucleotides HMPREF1078_RS00320 and HMPREF1078_RS00325 are separated by 1 nucleotides HMPREF1078_RS00325 and HMPREF1078_RS00330 are separated by 4 nucleotides
HMPREF1078_RS00315: HMPREF1078_RS00315 - aspartate--tRNA ligase, at 69,484 to 71,241
_RS00315
HMPREF1078_RS00320: HMPREF1078_RS00320 - Nif3-like dinuclear metal center hexameric protein, at 71,326 to 72,423
_RS00320
HMPREF1078_RS00325: HMPREF1078_RS00325 - C4-type zinc ribbon domain-containing protein, at 72,425 to 73,189
_RS00325
HMPREF1078_RS00330: HMPREF1078_RS00330 - tRNA lysidine(34) synthetase TilS, at 73,194 to 74,528
_RS00330
Position (kb)
71
72
73 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 71.213 kb on + strand at 71.225 kb on + strand at 71.229 kb on + strand at 71.229 kb on + strand at 71.229 kb on + strand at 71.229 kb on + strand at 71.229 kb on + strand at 71.230 kb on - strand at 71.239 kb on + strand at 71.239 kb on + strand at 71.240 kb on - strand at 71.242 kb on + strand at 71.242 kb on + strand at 71.243 kb on - strand at 71.243 kb on - strand at 71.243 kb on - strand at 71.243 kb on - strand at 71.244 kb on + strand at 71.244 kb on + strand at 71.244 kb on + strand at 71.245 kb on - strand at 71.251 kb on - strand at 71.251 kb on - strand at 71.292 kb on - strand at 71.314 kb on - strand at 71.318 kb on + strand at 71.326 kb on - strand at 71.344 kb on - strand at 71.345 kb on + strand at 71.346 kb on - strand at 71.346 kb on - strand at 71.346 kb on - strand at 71.346 kb on - strand at 71.365 kb on + strand at 71.502 kb on + strand, within HMPREF1078_RS00320 at 71.503 kb on - strand, within HMPREF1078_RS00320 at 71.566 kb on + strand, within HMPREF1078_RS00320 at 71.567 kb on - strand, within HMPREF1078_RS00320 at 71.567 kb on - strand, within HMPREF1078_RS00320 at 71.567 kb on - strand, within HMPREF1078_RS00320 at 71.599 kb on + strand, within HMPREF1078_RS00320 at 71.599 kb on - strand, within HMPREF1078_RS00320 at 71.600 kb on - strand, within HMPREF1078_RS00320 at 71.600 kb on - strand, within HMPREF1078_RS00320 at 71.614 kb on + strand, within HMPREF1078_RS00320 at 71.719 kb on - strand, within HMPREF1078_RS00320 at 71.755 kb on + strand, within HMPREF1078_RS00320 at 71.823 kb on + strand, within HMPREF1078_RS00320 at 71.892 kb on - strand, within HMPREF1078_RS00320 at 71.950 kb on - strand, within HMPREF1078_RS00320 at 71.989 kb on + strand, within HMPREF1078_RS00320 at 72.221 kb on - strand, within HMPREF1078_RS00320 at 72.257 kb on - strand, within HMPREF1078_RS00320 at 72.268 kb on + strand, within HMPREF1078_RS00320 at 72.311 kb on - strand, within HMPREF1078_RS00320 at 72.328 kb on - strand at 72.336 kb on + strand at 73.179 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction .-Glucan from yeast 2.5 mg/mL (C) remove 71,213 + +0.6 71,225 + +2.3 71,229 + +2.3 71,229 + -0.9 71,229 + +0.1 71,229 + +0.6 71,229 + -0.2 71,230 - -0.8 71,239 + +0.5 71,239 + +0.5 71,240 - -0.1 71,242 + -1.9 71,242 + -1.9 71,243 - -0.4 71,243 - +2.4 71,243 - -0.5 71,243 - +0.9 71,244 + -1.3 71,244 + -0.1 71,244 + +0.8 71,245 - +0.5 71,251 - +0.6 71,251 - -0.5 71,292 - +0.8 71,314 - +1.4 71,318 + -2.7 71,326 - +0.9 71,344 - -0.8 71,345 + +1.3 71,346 - +0.9 71,346 - -3.0 71,346 - -0.2 71,346 - -1.1 71,365 + +0.2 71,502 + HMPREF1078_RS00320 0.16 +0.4 71,503 - HMPREF1078_RS00320 0.16 +3.8 71,566 + HMPREF1078_RS00320 0.22 -1.7 71,567 - HMPREF1078_RS00320 0.22 -0.9 71,567 - HMPREF1078_RS00320 0.22 +1.0 71,567 - HMPREF1078_RS00320 0.22 -0.8 71,599 + HMPREF1078_RS00320 0.25 +0.6 71,599 - HMPREF1078_RS00320 0.25 -0.9 71,600 - HMPREF1078_RS00320 0.25 +1.2 71,600 - HMPREF1078_RS00320 0.25 +0.2 71,614 + HMPREF1078_RS00320 0.26 +1.1 71,719 - HMPREF1078_RS00320 0.36 -0.6 71,755 + HMPREF1078_RS00320 0.39 -1.3 71,823 + HMPREF1078_RS00320 0.45 +0.6 71,892 - HMPREF1078_RS00320 0.52 +0.2 71,950 - HMPREF1078_RS00320 0.57 -2.2 71,989 + HMPREF1078_RS00320 0.60 -3.3 72,221 - HMPREF1078_RS00320 0.82 +0.4 72,257 - HMPREF1078_RS00320 0.85 -0.7 72,268 + HMPREF1078_RS00320 0.86 -2.7 72,311 - HMPREF1078_RS00320 0.90 -2.5 72,328 - -0.8 72,336 + -0.1 73,179 + +1.4
Or see this region's nucleotide sequence