Experiment: Mucin from porcine stomach type III 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS01305 and HMPREF1078_RS01310 overlap by 4 nucleotides HMPREF1078_RS01310 and HMPREF1078_RS01315 are separated by 40 nucleotides HMPREF1078_RS01315 and HMPREF1078_RS01320 are separated by 89 nucleotides
HMPREF1078_RS01305: HMPREF1078_RS01305 - (Fe-S)-binding protein, at 340,936 to 341,676
_RS01305
HMPREF1078_RS01310: HMPREF1078_RS01310 - lactate utilization protein B, at 341,673 to 343,040
_RS01310
HMPREF1078_RS01315: HMPREF1078_RS01315 - LUD domain-containing protein, at 343,081 to 343,659
_RS01315
HMPREF1078_RS01320: HMPREF1078_RS01320 - GlsB/YeaQ/YmgE family stress response membrane protein, at 343,749 to 344,000
_RS01320
Position (kb) 341
342
343
344 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 340.677 kb on - strand at 340.679 kb on - strand at 340.679 kb on - strand at 340.681 kb on + strand at 340.681 kb on + strand at 340.681 kb on + strand at 340.681 kb on + strand at 340.681 kb on + strand at 340.682 kb on - strand at 340.682 kb on - strand at 340.682 kb on - strand at 340.685 kb on + strand at 340.686 kb on - strand at 340.687 kb on + strand at 340.687 kb on + strand at 340.688 kb on - strand at 340.724 kb on - strand at 340.822 kb on - strand at 340.822 kb on - strand at 340.851 kb on - strand at 340.949 kb on + strand at 340.950 kb on - strand at 340.950 kb on - strand at 340.954 kb on - strand at 340.964 kb on - strand at 340.964 kb on - strand at 340.966 kb on - strand at 340.976 kb on + strand at 340.977 kb on - strand at 341.005 kb on - strand at 341.146 kb on + strand, within HMPREF1078_RS01305 at 341.146 kb on + strand, within HMPREF1078_RS01305 at 341.147 kb on - strand, within HMPREF1078_RS01305 at 341.147 kb on - strand, within HMPREF1078_RS01305 at 341.229 kb on + strand, within HMPREF1078_RS01305 at 341.249 kb on - strand, within HMPREF1078_RS01305 at 341.249 kb on - strand, within HMPREF1078_RS01305 at 341.252 kb on + strand, within HMPREF1078_RS01305 at 341.253 kb on + strand, within HMPREF1078_RS01305 at 341.254 kb on - strand, within HMPREF1078_RS01305 at 341.319 kb on - strand, within HMPREF1078_RS01305 at 341.331 kb on + strand, within HMPREF1078_RS01305 at 341.331 kb on + strand, within HMPREF1078_RS01305 at 341.332 kb on - strand, within HMPREF1078_RS01305 at 341.382 kb on + strand, within HMPREF1078_RS01305 at 341.438 kb on + strand, within HMPREF1078_RS01305 at 341.518 kb on - strand, within HMPREF1078_RS01305 at 341.563 kb on + strand, within HMPREF1078_RS01305 at 341.564 kb on - strand, within HMPREF1078_RS01305 at 341.566 kb on - strand, within HMPREF1078_RS01305 at 341.598 kb on + strand, within HMPREF1078_RS01305 at 341.599 kb on - strand, within HMPREF1078_RS01305 at 341.599 kb on - strand, within HMPREF1078_RS01305 at 341.646 kb on + strand at 341.647 kb on - strand at 341.647 kb on - strand at 341.657 kb on + strand at 341.660 kb on + strand at 341.936 kb on + strand, within HMPREF1078_RS01310 at 341.937 kb on - strand, within HMPREF1078_RS01310 at 341.978 kb on + strand, within HMPREF1078_RS01310 at 342.020 kb on + strand, within HMPREF1078_RS01310 at 342.053 kb on + strand, within HMPREF1078_RS01310 at 342.053 kb on + strand, within HMPREF1078_RS01310 at 342.056 kb on - strand, within HMPREF1078_RS01310 at 342.080 kb on + strand, within HMPREF1078_RS01310 at 342.081 kb on - strand, within HMPREF1078_RS01310 at 342.208 kb on - strand, within HMPREF1078_RS01310 at 342.267 kb on - strand, within HMPREF1078_RS01310 at 342.267 kb on - strand, within HMPREF1078_RS01310 at 342.468 kb on + strand, within HMPREF1078_RS01310 at 342.469 kb on - strand, within HMPREF1078_RS01310 at 342.510 kb on + strand, within HMPREF1078_RS01310 at 342.513 kb on - strand, within HMPREF1078_RS01310 at 342.513 kb on - strand, within HMPREF1078_RS01310 at 342.513 kb on - strand, within HMPREF1078_RS01310 at 342.521 kb on - strand, within HMPREF1078_RS01310 at 342.521 kb on - strand, within HMPREF1078_RS01310 at 342.542 kb on + strand, within HMPREF1078_RS01310 at 342.600 kb on - strand, within HMPREF1078_RS01310 at 342.605 kb on + strand, within HMPREF1078_RS01310 at 342.606 kb on - strand, within HMPREF1078_RS01310 at 342.637 kb on - strand, within HMPREF1078_RS01310 at 342.642 kb on + strand, within HMPREF1078_RS01310 at 342.642 kb on + strand, within HMPREF1078_RS01310 at 342.643 kb on - strand, within HMPREF1078_RS01310 at 342.644 kb on + strand, within HMPREF1078_RS01310 at 342.644 kb on + strand, within HMPREF1078_RS01310 at 342.644 kb on + strand, within HMPREF1078_RS01310 at 342.644 kb on + strand, within HMPREF1078_RS01310 at 342.645 kb on - strand, within HMPREF1078_RS01310 at 342.645 kb on - strand, within HMPREF1078_RS01310 at 342.645 kb on - strand, within HMPREF1078_RS01310 at 342.645 kb on - strand, within HMPREF1078_RS01310 at 342.645 kb on - strand, within HMPREF1078_RS01310 at 342.645 kb on - strand, within HMPREF1078_RS01310 at 342.648 kb on + strand, within HMPREF1078_RS01310 at 342.648 kb on + strand, within HMPREF1078_RS01310 at 342.648 kb on + strand, within HMPREF1078_RS01310 at 342.649 kb on - strand, within HMPREF1078_RS01310 at 342.649 kb on - strand, within HMPREF1078_RS01310 at 342.649 kb on - strand, within HMPREF1078_RS01310 at 342.654 kb on - strand, within HMPREF1078_RS01310 at 342.769 kb on + strand, within HMPREF1078_RS01310 at 342.770 kb on - strand, within HMPREF1078_RS01310 at 342.789 kb on + strand, within HMPREF1078_RS01310 at 342.789 kb on + strand, within HMPREF1078_RS01310 at 342.813 kb on - strand, within HMPREF1078_RS01310 at 342.813 kb on - strand, within HMPREF1078_RS01310 at 342.813 kb on - strand, within HMPREF1078_RS01310 at 342.846 kb on - strand, within HMPREF1078_RS01310 at 343.033 kb on + strand at 343.043 kb on + strand at 343.044 kb on - strand at 343.044 kb on - strand at 343.054 kb on + strand at 343.054 kb on + strand at 343.055 kb on - strand at 343.055 kb on - strand at 343.055 kb on - strand at 343.086 kb on + strand at 343.098 kb on + strand at 343.098 kb on + strand at 343.100 kb on + strand at 343.101 kb on - strand at 343.101 kb on - strand at 343.101 kb on - strand at 343.103 kb on + strand at 343.103 kb on + strand at 343.103 kb on + strand at 343.103 kb on + strand at 343.103 kb on + strand at 343.141 kb on - strand, within HMPREF1078_RS01315 at 343.171 kb on + strand, within HMPREF1078_RS01315 at 343.171 kb on + strand, within HMPREF1078_RS01315 at 343.263 kb on + strand, within HMPREF1078_RS01315 at 343.264 kb on - strand, within HMPREF1078_RS01315 at 343.332 kb on + strand, within HMPREF1078_RS01315 at 343.371 kb on + strand, within HMPREF1078_RS01315 at 343.371 kb on + strand, within HMPREF1078_RS01315 at 343.371 kb on + strand, within HMPREF1078_RS01315 at 343.407 kb on - strand, within HMPREF1078_RS01315 at 343.442 kb on + strand, within HMPREF1078_RS01315 at 343.450 kb on - strand, within HMPREF1078_RS01315 at 343.464 kb on + strand, within HMPREF1078_RS01315 at 343.464 kb on + strand, within HMPREF1078_RS01315 at 343.465 kb on - strand, within HMPREF1078_RS01315 at 343.515 kb on + strand, within HMPREF1078_RS01315 at 343.528 kb on + strand, within HMPREF1078_RS01315 at 343.528 kb on + strand, within HMPREF1078_RS01315 at 343.552 kb on + strand, within HMPREF1078_RS01315 at 343.584 kb on + strand, within HMPREF1078_RS01315 at 343.598 kb on + strand, within HMPREF1078_RS01315 at 343.598 kb on + strand, within HMPREF1078_RS01315 at 343.598 kb on + strand, within HMPREF1078_RS01315 at 343.599 kb on - strand, within HMPREF1078_RS01315 at 343.613 kb on + strand at 343.613 kb on + strand at 343.614 kb on - strand at 343.640 kb on + strand at 343.640 kb on + strand at 343.657 kb on + strand at 343.728 kb on - strand at 343.732 kb on + strand at 343.732 kb on + strand at 343.734 kb on + strand at 343.735 kb on - strand at 343.735 kb on - strand at 343.735 kb on - strand at 343.735 kb on - strand at 343.751 kb on - strand at 343.751 kb on - strand at 343.789 kb on - strand, within HMPREF1078_RS01320 at 343.881 kb on + strand, within HMPREF1078_RS01320 at 343.968 kb on + strand, within HMPREF1078_RS01320 at 343.970 kb on + strand, within HMPREF1078_RS01320 at 343.970 kb on + strand, within HMPREF1078_RS01320 at 343.970 kb on + strand, within HMPREF1078_RS01320 at 343.974 kb on + strand, within HMPREF1078_RS01320 at 343.975 kb on - strand at 343.975 kb on - strand at 343.975 kb on - strand at 343.984 kb on + strand at 343.990 kb on - strand at 344.000 kb on + strand at 344.023 kb on - strand at 344.023 kb on - strand at 344.027 kb on + strand at 344.031 kb on + strand at 344.032 kb on - strand at 344.032 kb on - strand at 344.033 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mucin from porcine stomach type III 2.5 mg/mL (C) remove 340,677 - -0.2 340,679 - +0.9 340,679 - +0.3 340,681 + +0.5 340,681 + +0.1 340,681 + +0.1 340,681 + +0.5 340,681 + +0.1 340,682 - +0.3 340,682 - -0.8 340,682 - -1.0 340,685 + +0.3 340,686 - +0.6 340,687 + -0.0 340,687 + -0.7 340,688 - +0.7 340,724 - +0.1 340,822 - +0.4 340,822 - +0.4 340,851 - +0.2 340,949 + +0.1 340,950 - -0.4 340,950 - +0.1 340,954 - -1.2 340,964 - -0.7 340,964 - +0.9 340,966 - -1.0 340,976 + +1.4 340,977 - +0.3 341,005 - +0.4 341,146 + HMPREF1078_RS01305 0.28 -0.1 341,146 + HMPREF1078_RS01305 0.28 -1.3 341,147 - HMPREF1078_RS01305 0.28 -0.3 341,147 - HMPREF1078_RS01305 0.28 +0.2 341,229 + HMPREF1078_RS01305 0.40 -1.3 341,249 - HMPREF1078_RS01305 0.42 -4.3 341,249 - HMPREF1078_RS01305 0.42 +0.3 341,252 + HMPREF1078_RS01305 0.43 +1.6 341,253 + HMPREF1078_RS01305 0.43 +1.3 341,254 - HMPREF1078_RS01305 0.43 +0.6 341,319 - HMPREF1078_RS01305 0.52 +0.2 341,331 + HMPREF1078_RS01305 0.53 -0.8 341,331 + HMPREF1078_RS01305 0.53 -0.7 341,332 - HMPREF1078_RS01305 0.53 -0.0 341,382 + HMPREF1078_RS01305 0.60 -0.1 341,438 + HMPREF1078_RS01305 0.68 +0.9 341,518 - HMPREF1078_RS01305 0.79 -1.9 341,563 + HMPREF1078_RS01305 0.85 -1.5 341,564 - HMPREF1078_RS01305 0.85 -1.2 341,566 - HMPREF1078_RS01305 0.85 -0.4 341,598 + HMPREF1078_RS01305 0.89 -1.2 341,599 - HMPREF1078_RS01305 0.89 -0.2 341,599 - HMPREF1078_RS01305 0.89 +0.3 341,646 + +0.3 341,647 - +0.7 341,647 - +0.2 341,657 + +0.4 341,660 + +0.1 341,936 + HMPREF1078_RS01310 0.19 -0.5 341,937 - HMPREF1078_RS01310 0.19 -0.4 341,978 + HMPREF1078_RS01310 0.22 -0.6 342,020 + HMPREF1078_RS01310 0.25 -0.7 342,053 + HMPREF1078_RS01310 0.28 +1.3 342,053 + HMPREF1078_RS01310 0.28 -1.2 342,056 - HMPREF1078_RS01310 0.28 -1.1 342,080 + HMPREF1078_RS01310 0.30 +0.4 342,081 - HMPREF1078_RS01310 0.30 -1.8 342,208 - HMPREF1078_RS01310 0.39 -0.0 342,267 - HMPREF1078_RS01310 0.43 -0.4 342,267 - HMPREF1078_RS01310 0.43 -0.1 342,468 + HMPREF1078_RS01310 0.58 -0.2 342,469 - HMPREF1078_RS01310 0.58 -0.3 342,510 + HMPREF1078_RS01310 0.61 -0.4 342,513 - HMPREF1078_RS01310 0.61 -0.7 342,513 - HMPREF1078_RS01310 0.61 -1.4 342,513 - HMPREF1078_RS01310 0.61 +0.8 342,521 - HMPREF1078_RS01310 0.62 +0.8 342,521 - HMPREF1078_RS01310 0.62 -0.5 342,542 + HMPREF1078_RS01310 0.64 -2.3 342,600 - HMPREF1078_RS01310 0.68 -0.0 342,605 + HMPREF1078_RS01310 0.68 -0.4 342,606 - HMPREF1078_RS01310 0.68 +0.5 342,637 - HMPREF1078_RS01310 0.70 -0.1 342,642 + HMPREF1078_RS01310 0.71 +0.1 342,642 + HMPREF1078_RS01310 0.71 -1.0 342,643 - HMPREF1078_RS01310 0.71 +1.1 342,644 + HMPREF1078_RS01310 0.71 -0.3 342,644 + HMPREF1078_RS01310 0.71 -0.2 342,644 + HMPREF1078_RS01310 0.71 +0.5 342,644 + HMPREF1078_RS01310 0.71 +0.9 342,645 - HMPREF1078_RS01310 0.71 -0.2 342,645 - HMPREF1078_RS01310 0.71 +0.5 342,645 - HMPREF1078_RS01310 0.71 -0.9 342,645 - HMPREF1078_RS01310 0.71 -0.1 342,645 - HMPREF1078_RS01310 0.71 -0.5 342,645 - HMPREF1078_RS01310 0.71 -0.9 342,648 + HMPREF1078_RS01310 0.71 -0.2 342,648 + HMPREF1078_RS01310 0.71 -1.7 342,648 + HMPREF1078_RS01310 0.71 +0.0 342,649 - HMPREF1078_RS01310 0.71 -0.6 342,649 - HMPREF1078_RS01310 0.71 -3.1 342,649 - HMPREF1078_RS01310 0.71 -1.1 342,654 - HMPREF1078_RS01310 0.72 +1.1 342,769 + HMPREF1078_RS01310 0.80 +0.6 342,770 - HMPREF1078_RS01310 0.80 -0.7 342,789 + HMPREF1078_RS01310 0.82 -2.1 342,789 + HMPREF1078_RS01310 0.82 +0.5 342,813 - HMPREF1078_RS01310 0.83 +0.3 342,813 - HMPREF1078_RS01310 0.83 -1.3 342,813 - HMPREF1078_RS01310 0.83 -0.8 342,846 - HMPREF1078_RS01310 0.86 -1.3 343,033 + -1.3 343,043 + +0.3 343,044 - +0.3 343,044 - +0.5 343,054 + -0.6 343,054 + -1.5 343,055 - -0.3 343,055 - +0.4 343,055 - +0.2 343,086 + -1.1 343,098 + -0.0 343,098 + -1.1 343,100 + -1.0 343,101 - -0.1 343,101 - +0.1 343,101 - +0.6 343,103 + +0.1 343,103 + -0.3 343,103 + -0.1 343,103 + -1.3 343,103 + -0.7 343,141 - HMPREF1078_RS01315 0.10 +0.1 343,171 + HMPREF1078_RS01315 0.16 -0.6 343,171 + HMPREF1078_RS01315 0.16 -1.3 343,263 + HMPREF1078_RS01315 0.31 -2.7 343,264 - HMPREF1078_RS01315 0.32 +0.4 343,332 + HMPREF1078_RS01315 0.43 -0.7 343,371 + HMPREF1078_RS01315 0.50 +0.8 343,371 + HMPREF1078_RS01315 0.50 -0.2 343,371 + HMPREF1078_RS01315 0.50 +0.7 343,407 - HMPREF1078_RS01315 0.56 -0.4 343,442 + HMPREF1078_RS01315 0.62 -0.3 343,450 - HMPREF1078_RS01315 0.64 -0.4 343,464 + HMPREF1078_RS01315 0.66 +0.2 343,464 + HMPREF1078_RS01315 0.66 -1.6 343,465 - HMPREF1078_RS01315 0.66 -0.7 343,515 + HMPREF1078_RS01315 0.75 -0.9 343,528 + HMPREF1078_RS01315 0.77 -0.9 343,528 + HMPREF1078_RS01315 0.77 -0.4 343,552 + HMPREF1078_RS01315 0.81 -1.0 343,584 + HMPREF1078_RS01315 0.87 +1.0 343,598 + HMPREF1078_RS01315 0.89 -1.6 343,598 + HMPREF1078_RS01315 0.89 -0.3 343,598 + HMPREF1078_RS01315 0.89 -0.4 343,599 - HMPREF1078_RS01315 0.89 +1.4 343,613 + -1.7 343,613 + +0.4 343,614 - +0.1 343,640 + -0.3 343,640 + -0.5 343,657 + +0.6 343,728 - -2.1 343,732 + -2.7 343,732 + +1.4 343,734 + -1.1 343,735 - +0.3 343,735 - +0.7 343,735 - +0.5 343,735 - -0.5 343,751 - +0.3 343,751 - -0.5 343,789 - HMPREF1078_RS01320 0.16 -0.7 343,881 + HMPREF1078_RS01320 0.52 +1.2 343,968 + HMPREF1078_RS01320 0.87 +0.7 343,970 + HMPREF1078_RS01320 0.88 +0.1 343,970 + HMPREF1078_RS01320 0.88 +0.2 343,970 + HMPREF1078_RS01320 0.88 -1.6 343,974 + HMPREF1078_RS01320 0.89 -1.3 343,975 - -0.2 343,975 - -0.7 343,975 - +1.0 343,984 + -2.0 343,990 - +0.1 344,000 + +1.7 344,023 - +0.3 344,023 - +0.5 344,027 + +0.1 344,031 + +2.3 344,032 - +0.2 344,032 - -0.1 344,033 + -1.9
Or see this region's nucleotide sequence