Experiment: MoLS4 without vitamins
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1703 and DVU1704 are separated by 178 nucleotides DVU1704 and DVU1705 are separated by 26 nucleotides DVU1705 and DVU1707 are separated by 49 nucleotides DVU1707 and DVU1708 are separated by 2 nucleotides
DVU1703: DVU1703 - type I restriction-modification enzyme, R subunit (TIGR), at 1,777,728 to 1,781,027
DVU1703
DVU1704: DVU1704 - conserved hypothetical protein (TIGR), at 1,781,206 to 1,781,433
DVU1704
DVU1705: DVU1705 - type I restriction-modification enzyme, S subunit (TIGR), at 1,781,460 to 1,782,710
DVU1705
DVU1707: DVU1707 - hypothetical protein (TIGR), at 1,782,760 to 1,782,969
DVU1707
DVU1708: DVU1708 - conserved hypothetical protein (TIGR), at 1,782,972 to 1,783,985
DVU1708
Position (kb)
1781
1782
1783 Strain fitness (log2 ratio)
-1
0
1 at 1780.528 kb on + strand, within DVU1703 at 1780.660 kb on - strand, within DVU1703 at 1780.758 kb on + strand at 1780.758 kb on + strand at 1780.766 kb on - strand at 1780.766 kb on - strand at 1780.770 kb on + strand at 1780.842 kb on - strand at 1780.868 kb on - strand at 1780.887 kb on - strand at 1780.915 kb on + strand at 1780.962 kb on + strand at 1781.003 kb on - strand at 1781.058 kb on - strand at 1781.139 kb on - strand at 1781.166 kb on - strand at 1781.168 kb on + strand at 1781.298 kb on - strand, within DVU1704 at 1781.428 kb on + strand at 1781.443 kb on - strand at 1781.461 kb on + strand at 1781.496 kb on - strand at 1781.528 kb on - strand at 1781.528 kb on - strand at 1781.607 kb on - strand, within DVU1705 at 1782.188 kb on - strand, within DVU1705 at 1782.472 kb on - strand, within DVU1705 at 1783.091 kb on - strand, within DVU1708 at 1783.170 kb on + strand, within DVU1708 at 1783.266 kb on - strand, within DVU1708 at 1783.296 kb on - strand, within DVU1708 at 1783.555 kb on + strand, within DVU1708 at 1783.665 kb on + strand, within DVU1708
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins remove 1,780,528 + DVU1703 0.85 -1.5 1,780,660 - DVU1703 0.89 +0.1 1,780,758 + +0.5 1,780,758 + -0.8 1,780,766 - -0.4 1,780,766 - +0.6 1,780,770 + +0.2 1,780,842 - -0.1 1,780,868 - -0.0 1,780,887 - +0.7 1,780,915 + -0.4 1,780,962 + -0.1 1,781,003 - -0.3 1,781,058 - -0.3 1,781,139 - +0.9 1,781,166 - -0.2 1,781,168 + -0.6 1,781,298 - DVU1704 0.40 -0.3 1,781,428 + -0.7 1,781,443 - -0.6 1,781,461 + +0.4 1,781,496 - +0.5 1,781,528 - -0.1 1,781,528 - -0.8 1,781,607 - DVU1705 0.12 -0.2 1,782,188 - DVU1705 0.58 +1.0 1,782,472 - DVU1705 0.81 +0.3 1,783,091 - DVU1708 0.12 -0.2 1,783,170 + DVU1708 0.20 +0.2 1,783,266 - DVU1708 0.29 +0.4 1,783,296 - DVU1708 0.32 +0.0 1,783,555 + DVU1708 0.57 -0.0 1,783,665 + DVU1708 0.68 +1.1
Or see this region's nucleotide sequence