Experiment: MoLS4 without vitamins
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glk and DVU1036 overlap by 4 nucleotides DVU1036 and DVU1037 are separated by 30 nucleotides DVU1037 and hisA are separated by 102 nucleotides hisA and DVU1039 overlap by 4 nucleotides
DVU1035: glk - glucokinase, putative (TIGR), at 1,137,931 to 1,138,995
glk
DVU1036: DVU1036 - membrane protein, putative (TIGR), at 1,138,992 to 1,139,876
DVU1036
DVU1037: DVU1037 - mercuric reductase, putative (TIGR), at 1,139,907 to 1,141,349
DVU1037
DVU1038: hisA - phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (TIGR), at 1,141,452 to 1,142,201
hisA
DVU1039: DVU1039 - lipoprotein, putative (TIGR), at 1,142,198 to 1,142,839
DVU1039
Position (kb)
1139
1140
1141
1142 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1138.933 kb on + strand at 1138.968 kb on + strand at 1139.084 kb on - strand, within DVU1036 at 1139.102 kb on + strand, within DVU1036 at 1139.112 kb on - strand, within DVU1036 at 1139.124 kb on - strand, within DVU1036 at 1139.139 kb on + strand, within DVU1036 at 1139.165 kb on - strand, within DVU1036 at 1139.175 kb on + strand, within DVU1036 at 1139.176 kb on + strand, within DVU1036 at 1139.238 kb on - strand, within DVU1036 at 1139.294 kb on + strand, within DVU1036 at 1139.390 kb on - strand, within DVU1036 at 1139.487 kb on + strand, within DVU1036 at 1139.487 kb on + strand, within DVU1036 at 1139.561 kb on + strand, within DVU1036 at 1139.584 kb on - strand, within DVU1036 at 1139.605 kb on + strand, within DVU1036 at 1139.737 kb on - strand, within DVU1036 at 1139.794 kb on + strand at 1139.808 kb on - strand at 1139.870 kb on + strand at 1139.885 kb on + strand at 1140.039 kb on + strand at 1140.149 kb on + strand, within DVU1037 at 1140.149 kb on + strand, within DVU1037 at 1140.157 kb on - strand, within DVU1037 at 1140.179 kb on + strand, within DVU1037 at 1140.180 kb on - strand, within DVU1037 at 1140.187 kb on - strand, within DVU1037 at 1140.232 kb on + strand, within DVU1037 at 1140.270 kb on - strand, within DVU1037 at 1140.347 kb on - strand, within DVU1037 at 1140.373 kb on + strand, within DVU1037 at 1140.413 kb on - strand, within DVU1037 at 1140.576 kb on - strand, within DVU1037 at 1140.641 kb on + strand, within DVU1037 at 1140.717 kb on - strand, within DVU1037 at 1140.729 kb on + strand, within DVU1037 at 1140.730 kb on + strand, within DVU1037 at 1140.814 kb on + strand, within DVU1037 at 1140.949 kb on - strand, within DVU1037 at 1140.952 kb on - strand, within DVU1037 at 1140.968 kb on - strand, within DVU1037 at 1141.150 kb on - strand, within DVU1037 at 1141.163 kb on - strand, within DVU1037 at 1141.189 kb on + strand, within DVU1037 at 1141.197 kb on - strand, within DVU1037 at 1141.197 kb on - strand, within DVU1037 at 1141.217 kb on + strand at 1141.217 kb on + strand at 1141.218 kb on + strand at 1141.228 kb on + strand at 1141.236 kb on - strand at 1141.447 kb on - strand at 1141.478 kb on + strand at 1141.486 kb on - strand at 1141.503 kb on - strand at 1141.561 kb on + strand, within hisA at 1141.631 kb on - strand, within hisA at 1141.633 kb on + strand, within hisA at 1141.633 kb on + strand, within hisA at 1141.633 kb on + strand, within hisA at 1141.641 kb on - strand, within hisA at 1141.743 kb on + strand, within hisA at 1142.159 kb on + strand at 1142.220 kb on - strand at 1142.302 kb on - strand, within DVU1039
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins remove 1,138,933 + +1.4 1,138,968 + -0.2 1,139,084 - DVU1036 0.10 -0.6 1,139,102 + DVU1036 0.12 +0.1 1,139,112 - DVU1036 0.14 -0.1 1,139,124 - DVU1036 0.15 +0.7 1,139,139 + DVU1036 0.17 -0.6 1,139,165 - DVU1036 0.20 +0.2 1,139,175 + DVU1036 0.21 -0.1 1,139,176 + DVU1036 0.21 +0.7 1,139,238 - DVU1036 0.28 -0.4 1,139,294 + DVU1036 0.34 -0.9 1,139,390 - DVU1036 0.45 +0.1 1,139,487 + DVU1036 0.56 -0.1 1,139,487 + DVU1036 0.56 +0.4 1,139,561 + DVU1036 0.64 +0.6 1,139,584 - DVU1036 0.67 +0.5 1,139,605 + DVU1036 0.69 +0.1 1,139,737 - DVU1036 0.84 -0.5 1,139,794 + -0.0 1,139,808 - +0.1 1,139,870 + -0.4 1,139,885 + +0.7 1,140,039 + +0.5 1,140,149 + DVU1037 0.17 +2.4 1,140,149 + DVU1037 0.17 +0.1 1,140,157 - DVU1037 0.17 +0.5 1,140,179 + DVU1037 0.19 +1.2 1,140,180 - DVU1037 0.19 -0.5 1,140,187 - DVU1037 0.19 +0.1 1,140,232 + DVU1037 0.23 -0.0 1,140,270 - DVU1037 0.25 -0.0 1,140,347 - DVU1037 0.30 -0.2 1,140,373 + DVU1037 0.32 -0.6 1,140,413 - DVU1037 0.35 +1.1 1,140,576 - DVU1037 0.46 +0.1 1,140,641 + DVU1037 0.51 +0.0 1,140,717 - DVU1037 0.56 -0.9 1,140,729 + DVU1037 0.57 -0.1 1,140,730 + DVU1037 0.57 +0.1 1,140,814 + DVU1037 0.63 +0.1 1,140,949 - DVU1037 0.72 +1.2 1,140,952 - DVU1037 0.72 -0.1 1,140,968 - DVU1037 0.74 +0.3 1,141,150 - DVU1037 0.86 +0.0 1,141,163 - DVU1037 0.87 +0.8 1,141,189 + DVU1037 0.89 -0.3 1,141,197 - DVU1037 0.89 -0.1 1,141,197 - DVU1037 0.89 -0.1 1,141,217 + +0.8 1,141,217 + +0.2 1,141,218 + -0.3 1,141,228 + +0.1 1,141,236 - -0.1 1,141,447 - -0.1 1,141,478 + -6.2 1,141,486 - -0.4 1,141,503 - -0.9 1,141,561 + hisA DVU1038 0.15 -1.5 1,141,631 - hisA DVU1038 0.24 -2.1 1,141,633 + hisA DVU1038 0.24 -2.2 1,141,633 + hisA DVU1038 0.24 +0.4 1,141,633 + hisA DVU1038 0.24 -1.2 1,141,641 - hisA DVU1038 0.25 -1.2 1,141,743 + hisA DVU1038 0.39 -2.4 1,142,159 + -0.8 1,142,220 - -1.1 1,142,302 - DVU1039 0.16 -3.0
Or see this region's nucleotide sequence