Experiment: Lactate-Thiosulfate (15mM) + 0.1% Yeast Extract (MOYLThioS4)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mutS and DVU1650 are separated by 163 nucleotides DVU1650 and DVU1651 are separated by 14 nucleotides DVU1651 and hit are separated by 10 nucleotides hit and DVU1653 are separated by 62 nucleotides
DVU1649: mutS - DNA mismatch repair protein MutS (TIGR), at 1,727,737 to 1,730,454
mutS
DVU1650: DVU1650 - conserved hypothetical protein (TIGR), at 1,730,618 to 1,731,742
DVU1650
DVU1651: DVU1651 - conserved hypothetical protein (TIGR), at 1,731,757 to 1,732,128
DVU1651
DVU1652: hit - HIT family protein (TIGR), at 1,732,139 to 1,732,663
hit
DVU1653: DVU1653 - polyA polymerase family protein (TIGR), at 1,732,726 to 1,735,440
DVU1653
Position (kb)
1730
1731
1732 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1729.670 kb on + strand, within mutS at 1729.670 kb on + strand, within mutS at 1729.670 kb on + strand, within mutS at 1729.680 kb on - strand, within mutS at 1729.764 kb on + strand, within mutS at 1730.071 kb on - strand, within mutS at 1730.203 kb on - strand at 1730.203 kb on - strand at 1730.387 kb on + strand at 1730.387 kb on + strand at 1730.390 kb on - strand at 1730.395 kb on - strand at 1730.514 kb on - strand at 1730.616 kb on + strand at 1730.624 kb on - strand at 1730.636 kb on + strand at 1730.776 kb on - strand, within DVU1650 at 1730.823 kb on - strand, within DVU1650 at 1730.843 kb on + strand, within DVU1650 at 1731.070 kb on + strand, within DVU1650 at 1731.082 kb on + strand, within DVU1650 at 1731.095 kb on - strand, within DVU1650 at 1731.174 kb on + strand, within DVU1650 at 1731.349 kb on + strand, within DVU1650 at 1731.370 kb on + strand, within DVU1650 at 1731.451 kb on + strand, within DVU1650 at 1731.459 kb on - strand, within DVU1650 at 1731.477 kb on + strand, within DVU1650 at 1731.580 kb on - strand, within DVU1650 at 1731.682 kb on - strand at 1731.705 kb on + strand at 1731.775 kb on - strand at 1731.882 kb on - strand, within DVU1651 at 1731.931 kb on - strand, within DVU1651 at 1731.951 kb on + strand, within DVU1651 at 1732.174 kb on - strand at 1732.177 kb on + strand at 1732.414 kb on + strand, within hit at 1732.518 kb on + strand, within hit at 1732.523 kb on + strand, within hit at 1732.526 kb on - strand, within hit at 1732.526 kb on - strand, within hit at 1732.723 kb on + strand at 1732.731 kb on - strand at 1732.731 kb on - strand at 1732.731 kb on - strand at 1732.731 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lactate-Thiosulfate (15mM) + 0.1% Yeast Extract (MOYLThioS4) remove 1,729,670 + mutS DVU1649 0.71 +0.6 1,729,670 + mutS DVU1649 0.71 +0.3 1,729,670 + mutS DVU1649 0.71 -0.1 1,729,680 - mutS DVU1649 0.71 -0.3 1,729,764 + mutS DVU1649 0.75 -0.1 1,730,071 - mutS DVU1649 0.86 -0.6 1,730,203 - -0.2 1,730,203 - -0.7 1,730,387 + -0.2 1,730,387 + -0.0 1,730,390 - -0.3 1,730,395 - -0.9 1,730,514 - -0.2 1,730,616 + -0.1 1,730,624 - -0.2 1,730,636 + -0.1 1,730,776 - DVU1650 0.14 -0.4 1,730,823 - DVU1650 0.18 -1.0 1,730,843 + DVU1650 0.20 -1.3 1,731,070 + DVU1650 0.40 +0.1 1,731,082 + DVU1650 0.41 -1.8 1,731,095 - DVU1650 0.42 +0.2 1,731,174 + DVU1650 0.49 -0.9 1,731,349 + DVU1650 0.65 -1.2 1,731,370 + DVU1650 0.67 -1.9 1,731,451 + DVU1650 0.74 -1.6 1,731,459 - DVU1650 0.75 +0.5 1,731,477 + DVU1650 0.76 -2.0 1,731,580 - DVU1650 0.86 -1.0 1,731,682 - -3.2 1,731,705 + -1.0 1,731,775 - -0.2 1,731,882 - DVU1651 0.34 -0.3 1,731,931 - DVU1651 0.47 -0.7 1,731,951 + DVU1651 0.52 -2.4 1,732,174 - -0.8 1,732,177 + -1.3 1,732,414 + hit DVU1652 0.52 -0.3 1,732,518 + hit DVU1652 0.72 -0.1 1,732,523 + hit DVU1652 0.73 +0.5 1,732,526 - hit DVU1652 0.74 +0.4 1,732,526 - hit DVU1652 0.74 +0.2 1,732,723 + -1.9 1,732,731 - -1.8 1,732,731 - -1.1 1,732,731 - -0.3 1,732,731 - +0.2
Or see this region's nucleotide sequence