Experiment: Lactate-Thiosulfate (15mM) minimal (MOLThioS4)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolQ-1 and tolR overlap by 17 nucleotides tolR and DVU2390 overlap by 4 nucleotides DVU2390 and tRNA-Asn are separated by 281 nucleotides tRNA-Asn and DVU2391 are separated by 151 nucleotides DVU2391 and DVU2392 are separated by 184 nucleotides
DVU2388: tolQ-1 - tolQ protein (TIGR), at 2,490,966 to 2,491,919
tolQ-1
DVU2389: tolR - biopolymer transport protein, ExbD/TolR family (TIGR), at 2,491,903 to 2,492,316
tolR
DVU2390: DVU2390 - TonB domain protein (TIGR), at 2,492,313 to 2,493,221
DVU2390
DVU_tRNA-Asn-2: tRNA-Asn - tRNA with anticodon GTT for Asn (VIMSS-AUTO), at 2,493,503 to 2,493,578
tRNA-Asn
DVU2391: DVU2391 - hypothetical protein (TIGR), at 2,493,730 to 2,493,873
DVU2391
DVU2392: DVU2392 - type III secretion chaperone, CesT family (TIGR), at 2,494,058 to 2,494,510
DVU2392
Position (kb)
2492
2493
2494 Strain fitness (log2 ratio)
-1
0
1
2 at 2491.373 kb on + strand, within tolQ-1 at 2491.373 kb on + strand, within tolQ-1 at 2491.383 kb on + strand, within tolQ-1 at 2491.383 kb on + strand, within tolQ-1 at 2491.421 kb on + strand, within tolQ-1 at 2491.429 kb on - strand, within tolQ-1 at 2491.429 kb on - strand, within tolQ-1 at 2491.532 kb on - strand, within tolQ-1 at 2491.578 kb on - strand, within tolQ-1 at 2491.905 kb on - strand at 2491.969 kb on + strand, within tolR at 2492.162 kb on + strand, within tolR at 2492.163 kb on + strand, within tolR at 2492.291 kb on - strand at 2492.312 kb on + strand at 2492.374 kb on + strand at 2492.374 kb on + strand at 2492.445 kb on - strand, within DVU2390 at 2492.450 kb on + strand, within DVU2390 at 2492.486 kb on + strand, within DVU2390 at 2492.494 kb on - strand, within DVU2390 at 2492.494 kb on - strand, within DVU2390 at 2492.552 kb on + strand, within DVU2390 at 2492.604 kb on - strand, within DVU2390 at 2492.636 kb on + strand, within DVU2390 at 2492.674 kb on - strand, within DVU2390 at 2492.693 kb on + strand, within DVU2390 at 2492.770 kb on + strand, within DVU2390 at 2492.819 kb on - strand, within DVU2390 at 2492.913 kb on + strand, within DVU2390 at 2492.934 kb on + strand, within DVU2390 at 2492.940 kb on + strand, within DVU2390 at 2492.950 kb on + strand, within DVU2390 at 2492.958 kb on - strand, within DVU2390 at 2493.003 kb on + strand, within DVU2390 at 2493.074 kb on - strand, within DVU2390 at 2493.363 kb on + strand at 2493.363 kb on + strand at 2493.376 kb on + strand at 2493.376 kb on + strand at 2493.384 kb on - strand at 2493.675 kb on + strand at 2493.832 kb on - strand, within DVU2391 at 2493.963 kb on + strand at 2493.983 kb on - strand at 2494.051 kb on - strand at 2494.170 kb on + strand, within DVU2392 at 2494.179 kb on - strand, within DVU2392 at 2494.199 kb on + strand, within DVU2392 at 2494.201 kb on - strand, within DVU2392 at 2494.214 kb on - strand, within DVU2392
Per-strain Table
Position Strand Gene LocusTag Fraction Lactate-Thiosulfate (15mM) minimal (MOLThioS4) remove 2,491,373 + tolQ-1 DVU2388 0.43 -1.6 2,491,373 + tolQ-1 DVU2388 0.43 +0.4 2,491,383 + tolQ-1 DVU2388 0.44 +1.2 2,491,383 + tolQ-1 DVU2388 0.44 -1.6 2,491,421 + tolQ-1 DVU2388 0.48 -0.3 2,491,429 - tolQ-1 DVU2388 0.49 +2.1 2,491,429 - tolQ-1 DVU2388 0.49 +0.4 2,491,532 - tolQ-1 DVU2388 0.59 +0.1 2,491,578 - tolQ-1 DVU2388 0.64 +0.2 2,491,905 - +0.2 2,491,969 + tolR DVU2389 0.16 +0.9 2,492,162 + tolR DVU2389 0.63 -0.8 2,492,163 + tolR DVU2389 0.63 +0.3 2,492,291 - -0.6 2,492,312 + -1.3 2,492,374 + -0.6 2,492,374 + -1.6 2,492,445 - DVU2390 0.15 +0.4 2,492,450 + DVU2390 0.15 +0.3 2,492,486 + DVU2390 0.19 -0.6 2,492,494 - DVU2390 0.20 +0.4 2,492,494 - DVU2390 0.20 +1.2 2,492,552 + DVU2390 0.26 -0.3 2,492,604 - DVU2390 0.32 +0.1 2,492,636 + DVU2390 0.36 -0.0 2,492,674 - DVU2390 0.40 -0.6 2,492,693 + DVU2390 0.42 -0.3 2,492,770 + DVU2390 0.50 -0.1 2,492,819 - DVU2390 0.56 +0.5 2,492,913 + DVU2390 0.66 +0.7 2,492,934 + DVU2390 0.68 +0.2 2,492,940 + DVU2390 0.69 +0.4 2,492,950 + DVU2390 0.70 +1.9 2,492,958 - DVU2390 0.71 -0.8 2,493,003 + DVU2390 0.76 -0.6 2,493,074 - DVU2390 0.84 +1.7 2,493,363 + -1.5 2,493,363 + +1.3 2,493,376 + -0.2 2,493,376 + -1.2 2,493,384 - +0.1 2,493,675 + -0.1 2,493,832 - DVU2391 0.71 +0.9 2,493,963 + -1.1 2,493,983 - +0.3 2,494,051 - -0.6 2,494,170 + DVU2392 0.25 +0.7 2,494,179 - DVU2392 0.27 -0.0 2,494,199 + DVU2392 0.31 +0.1 2,494,201 - DVU2392 0.32 -0.1 2,494,214 - DVU2392 0.34 +0.3
Or see this region's nucleotide sequence