Experiment: MoLS4 without Molybdate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuC and leuD are separated by 17 nucleotides leuD and DVU2984 are separated by 2 nucleotides DVU2984 and leuB are separated by 29 nucleotides
DVU2982: leuC - 3-isopropylmalate dehydratase / (R)-citramalate isomerase, large subunit LeuC (EC 4.2.1.33; EC 4.2.1.35) (from data) , at 3,089,372 to 3,090,631
leuC
DVU2983: leuD - 3-isopropylmalate dehydratase / (R)-citramalate isomerase, small subunit LeuD (EC 4.2.1.33; EC 4.2.1.35) (from data) , at 3,090,649 to 3,091,152
leuD
DVU2984: DVU2984 - conserved hypothetical protein (TIGR), at 3,091,155 to 3,092,162
DVU2984
DVU2985: leuB - 3-isopropylmalate dehydrogenase (TIGR), at 3,092,192 to 3,093,265
leuB
Position (kb)
3091
3092
3093 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 3090.164 kb on - strand, within leuC at 3090.178 kb on - strand, within leuC at 3090.348 kb on + strand, within leuC at 3090.412 kb on - strand, within leuC at 3090.565 kb on + strand at 3090.616 kb on + strand at 3090.618 kb on + strand at 3090.624 kb on - strand at 3090.766 kb on - strand, within leuD at 3090.777 kb on + strand, within leuD at 3090.787 kb on - strand, within leuD at 3090.939 kb on - strand, within leuD at 3091.059 kb on - strand, within leuD at 3091.066 kb on + strand, within leuD at 3091.074 kb on - strand, within leuD at 3091.114 kb on + strand at 3091.269 kb on - strand, within DVU2984 at 3091.291 kb on - strand, within DVU2984 at 3091.355 kb on + strand, within DVU2984 at 3091.402 kb on - strand, within DVU2984 at 3091.464 kb on + strand, within DVU2984 at 3091.472 kb on - strand, within DVU2984 at 3091.504 kb on + strand, within DVU2984 at 3091.569 kb on + strand, within DVU2984 at 3091.709 kb on - strand, within DVU2984 at 3091.716 kb on - strand, within DVU2984 at 3091.828 kb on - strand, within DVU2984 at 3091.870 kb on + strand, within DVU2984 at 3091.878 kb on + strand, within DVU2984 at 3091.918 kb on + strand, within DVU2984 at 3091.923 kb on + strand, within DVU2984 at 3091.931 kb on - strand, within DVU2984 at 3091.968 kb on + strand, within DVU2984 at 3092.003 kb on + strand, within DVU2984 at 3092.027 kb on + strand, within DVU2984 at 3092.034 kb on + strand, within DVU2984 at 3092.203 kb on + strand at 3092.261 kb on - strand at 3092.323 kb on - strand, within leuB at 3092.396 kb on + strand, within leuB at 3092.396 kb on + strand, within leuB at 3092.402 kb on - strand, within leuB at 3092.440 kb on - strand, within leuB at 3092.484 kb on + strand, within leuB at 3092.519 kb on - strand, within leuB at 3092.558 kb on + strand, within leuB at 3092.566 kb on - strand, within leuB at 3092.680 kb on - strand, within leuB at 3092.725 kb on - strand, within leuB at 3092.727 kb on + strand, within leuB at 3092.749 kb on + strand, within leuB at 3092.749 kb on + strand, within leuB at 3092.897 kb on - strand, within leuB at 3092.905 kb on - strand, within leuB at 3092.931 kb on - strand, within leuB at 3092.990 kb on + strand, within leuB at 3093.019 kb on + strand, within leuB at 3093.061 kb on + strand, within leuB at 3093.061 kb on + strand, within leuB at 3093.061 kb on + strand, within leuB at 3093.061 kb on + strand, within leuB at 3093.061 kb on + strand, within leuB at 3093.061 kb on + strand, within leuB at 3093.067 kb on + strand, within leuB at 3093.069 kb on - strand, within leuB at 3093.069 kb on - strand, within leuB at 3093.069 kb on - strand, within leuB at 3093.069 kb on - strand, within leuB at 3093.069 kb on - strand, within leuB at 3093.075 kb on - strand, within leuB at 3093.117 kb on + strand, within leuB
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without Molybdate remove 3,090,164 - leuC DVU2982 0.63 -4.3 3,090,178 - leuC DVU2982 0.64 -1.6 3,090,348 + leuC DVU2982 0.77 -5.7 3,090,412 - leuC DVU2982 0.83 -4.8 3,090,565 + +1.6 3,090,616 + +0.1 3,090,618 + -3.8 3,090,624 - -2.6 3,090,766 - leuD DVU2983 0.23 -6.1 3,090,777 + leuD DVU2983 0.25 -6.0 3,090,787 - leuD DVU2983 0.27 -4.5 3,090,939 - leuD DVU2983 0.58 -5.6 3,091,059 - leuD DVU2983 0.81 -4.7 3,091,066 + leuD DVU2983 0.83 -3.9 3,091,074 - leuD DVU2983 0.84 -2.5 3,091,114 + -2.6 3,091,269 - DVU2984 0.11 -2.4 3,091,291 - DVU2984 0.13 -1.5 3,091,355 + DVU2984 0.20 -0.9 3,091,402 - DVU2984 0.25 -2.8 3,091,464 + DVU2984 0.31 -1.6 3,091,472 - DVU2984 0.31 -1.7 3,091,504 + DVU2984 0.35 -1.0 3,091,569 + DVU2984 0.41 +0.2 3,091,709 - DVU2984 0.55 -1.6 3,091,716 - DVU2984 0.56 -1.0 3,091,828 - DVU2984 0.67 -0.1 3,091,870 + DVU2984 0.71 -1.2 3,091,878 + DVU2984 0.72 +0.3 3,091,918 + DVU2984 0.76 -0.1 3,091,923 + DVU2984 0.76 -0.3 3,091,931 - DVU2984 0.77 -0.6 3,091,968 + DVU2984 0.81 -0.2 3,092,003 + DVU2984 0.84 +1.4 3,092,027 + DVU2984 0.87 -0.6 3,092,034 + DVU2984 0.87 -0.2 3,092,203 + -1.9 3,092,261 - -5.2 3,092,323 - leuB DVU2985 0.12 -4.9 3,092,396 + leuB DVU2985 0.19 -5.4 3,092,396 + leuB DVU2985 0.19 -3.0 3,092,402 - leuB DVU2985 0.20 -4.7 3,092,440 - leuB DVU2985 0.23 -4.6 3,092,484 + leuB DVU2985 0.27 -4.0 3,092,519 - leuB DVU2985 0.30 -0.1 3,092,558 + leuB DVU2985 0.34 -2.9 3,092,566 - leuB DVU2985 0.35 -3.3 3,092,680 - leuB DVU2985 0.45 -0.6 3,092,725 - leuB DVU2985 0.50 -4.2 3,092,727 + leuB DVU2985 0.50 -5.1 3,092,749 + leuB DVU2985 0.52 -4.7 3,092,749 + leuB DVU2985 0.52 -4.0 3,092,897 - leuB DVU2985 0.66 -5.1 3,092,905 - leuB DVU2985 0.66 -1.8 3,092,931 - leuB DVU2985 0.69 -3.3 3,092,990 + leuB DVU2985 0.74 -4.7 3,093,019 + leuB DVU2985 0.77 -1.6 3,093,061 + leuB DVU2985 0.81 -2.4 3,093,061 + leuB DVU2985 0.81 -5.9 3,093,061 + leuB DVU2985 0.81 -5.1 3,093,061 + leuB DVU2985 0.81 -3.6 3,093,061 + leuB DVU2985 0.81 -5.3 3,093,061 + leuB DVU2985 0.81 -4.8 3,093,067 + leuB DVU2985 0.81 -3.0 3,093,069 - leuB DVU2985 0.82 -6.8 3,093,069 - leuB DVU2985 0.82 -2.8 3,093,069 - leuB DVU2985 0.82 -4.9 3,093,069 - leuB DVU2985 0.82 -3.0 3,093,069 - leuB DVU2985 0.82 -0.8 3,093,075 - leuB DVU2985 0.82 -4.4 3,093,117 + leuB DVU2985 0.86 -6.2
Or see this region's nucleotide sequence