Experiment: Lactate-Sulfite (30-20 mM) minimal (MoLS3)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2360 and thiE-2 are separated by 223 nucleotides thiE-2 and thiM overlap by 4 nucleotides
DVU2360: DVU2360 - oxidoreductase, FAD/NAD-binding family (TIGR), at 2,458,415 to 2,459,221
DVU2360
DVU2362: thiE-2 - thiamine-phosphate pyrophosphorylase (TIGR), at 2,459,445 to 2,460,104
thiE-2
DVU2363: thiM - hydroxyethylthiazole kinase (TIGR), at 2,460,101 to 2,460,904
thiM
Position (kb)
2459
2460
2461 Strain fitness (log2 ratio)
-1
0
1 at 2458.528 kb on + strand, within DVU2360 at 2458.558 kb on + strand, within DVU2360 at 2458.627 kb on + strand, within DVU2360 at 2458.634 kb on + strand, within DVU2360 at 2458.637 kb on - strand, within DVU2360 at 2458.677 kb on - strand, within DVU2360 at 2458.711 kb on - strand, within DVU2360 at 2458.801 kb on + strand, within DVU2360 at 2458.839 kb on - strand, within DVU2360 at 2458.895 kb on + strand, within DVU2360 at 2458.908 kb on - strand, within DVU2360 at 2458.930 kb on + strand, within DVU2360 at 2458.965 kb on + strand, within DVU2360 at 2458.973 kb on - strand, within DVU2360 at 2459.022 kb on - strand, within DVU2360 at 2459.022 kb on - strand, within DVU2360 at 2459.022 kb on - strand, within DVU2360 at 2459.086 kb on - strand, within DVU2360 at 2459.121 kb on - strand, within DVU2360 at 2459.180 kb on + strand at 2459.188 kb on - strand at 2459.204 kb on + strand at 2459.229 kb on + strand at 2459.237 kb on - strand at 2459.237 kb on - strand at 2459.329 kb on + strand at 2459.369 kb on + strand at 2459.370 kb on + strand at 2459.403 kb on - strand at 2459.440 kb on - strand at 2459.485 kb on - strand at 2459.578 kb on + strand, within thiE-2 at 2459.578 kb on + strand, within thiE-2 at 2459.727 kb on + strand, within thiE-2 at 2459.748 kb on - strand, within thiE-2 at 2459.978 kb on + strand, within thiE-2 at 2460.052 kb on - strand at 2460.061 kb on + strand at 2460.103 kb on - strand at 2460.122 kb on - strand at 2460.314 kb on + strand, within thiM at 2460.322 kb on - strand, within thiM at 2460.322 kb on - strand, within thiM at 2460.412 kb on - strand, within thiM at 2460.468 kb on + strand, within thiM at 2460.477 kb on - strand, within thiM at 2460.487 kb on - strand, within thiM at 2460.494 kb on - strand, within thiM at 2460.494 kb on - strand, within thiM at 2460.504 kb on + strand, within thiM at 2460.571 kb on - strand, within thiM at 2460.597 kb on + strand, within thiM at 2460.597 kb on + strand, within thiM at 2460.605 kb on - strand, within thiM at 2460.637 kb on + strand, within thiM at 2460.675 kb on - strand, within thiM at 2460.791 kb on - strand, within thiM at 2460.827 kb on - strand at 2460.892 kb on - strand at 2460.902 kb on + strand at 2460.902 kb on - strand at 2460.914 kb on + strand at 2460.947 kb on - strand at 2460.947 kb on - strand at 2461.065 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lactate-Sulfite (30-20 mM) minimal (MoLS3) remove 2,458,528 + DVU2360 0.14 +0.3 2,458,558 + DVU2360 0.18 +0.4 2,458,627 + DVU2360 0.26 -0.5 2,458,634 + DVU2360 0.27 -0.7 2,458,637 - DVU2360 0.28 -0.3 2,458,677 - DVU2360 0.32 -0.2 2,458,711 - DVU2360 0.37 -1.2 2,458,801 + DVU2360 0.48 -0.2 2,458,839 - DVU2360 0.53 -0.5 2,458,895 + DVU2360 0.59 -0.3 2,458,908 - DVU2360 0.61 -0.1 2,458,930 + DVU2360 0.64 -1.0 2,458,965 + DVU2360 0.68 -0.1 2,458,973 - DVU2360 0.69 +0.7 2,459,022 - DVU2360 0.75 -0.5 2,459,022 - DVU2360 0.75 -0.3 2,459,022 - DVU2360 0.75 +0.5 2,459,086 - DVU2360 0.83 -0.5 2,459,121 - DVU2360 0.87 -0.6 2,459,180 + -0.3 2,459,188 - +1.4 2,459,204 + -0.2 2,459,229 + +0.1 2,459,237 - -0.2 2,459,237 - -0.2 2,459,329 + -0.3 2,459,369 + -0.4 2,459,370 + +0.3 2,459,403 - +0.3 2,459,440 - -0.3 2,459,485 - -0.2 2,459,578 + thiE-2 DVU2362 0.20 -0.3 2,459,578 + thiE-2 DVU2362 0.20 +0.6 2,459,727 + thiE-2 DVU2362 0.43 -0.8 2,459,748 - thiE-2 DVU2362 0.46 -0.8 2,459,978 + thiE-2 DVU2362 0.81 -0.9 2,460,052 - -0.5 2,460,061 + -0.1 2,460,103 - +0.0 2,460,122 - -1.0 2,460,314 + thiM DVU2363 0.26 -0.3 2,460,322 - thiM DVU2363 0.27 -1.0 2,460,322 - thiM DVU2363 0.27 -1.0 2,460,412 - thiM DVU2363 0.39 -0.8 2,460,468 + thiM DVU2363 0.46 -1.1 2,460,477 - thiM DVU2363 0.47 -0.6 2,460,487 - thiM DVU2363 0.48 -0.5 2,460,494 - thiM DVU2363 0.49 -0.3 2,460,494 - thiM DVU2363 0.49 -1.1 2,460,504 + thiM DVU2363 0.50 +1.1 2,460,571 - thiM DVU2363 0.58 -0.5 2,460,597 + thiM DVU2363 0.62 -0.6 2,460,597 + thiM DVU2363 0.62 -0.6 2,460,605 - thiM DVU2363 0.63 -0.5 2,460,637 + thiM DVU2363 0.67 -0.3 2,460,675 - thiM DVU2363 0.71 -0.5 2,460,791 - thiM DVU2363 0.86 -0.9 2,460,827 - +0.2 2,460,892 - -0.8 2,460,902 + +0.2 2,460,902 - -0.5 2,460,914 + +0.1 2,460,947 - -0.2 2,460,947 - -0.3 2,461,065 + -0.8
Or see this region's nucleotide sequence