Experiment: L-Asparagine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3148 and Rv3149 overlap by 4 nucleotides Rv3149 and Rv3150 overlap by 4 nucleotides Rv3150 and Rv3151 overlap by 4 nucleotides
Rv3148: Rv3148 - Probable NADH dehydrogenase I (chain D) NuoD (NADH-ubiquinone oxidoreductase chain D), at 3,513,338 to 3,514,660
Rv3148
Rv3149: Rv3149 - Probable NADH dehydrogenase I (chain E) NuoE (NADH-ubiquinone oxidoreductase chain E), at 3,514,657 to 3,515,415
Rv3149
Rv3150: Rv3150 - Probable NADH dehydrogenase I (chain F) NuoF (NADH-ubiquinone oxidoreductase chain F), at 3,515,412 to 3,516,749
Rv3150
Rv3151: Rv3151 - Probable NADH dehydrogenase I (chain G) NuoG (NADH-ubiquinone oxidoreductase chain G), at 3,516,746 to 3,519,166
Rv3151
Position (kb)
3515
3516
3517 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3514.494 kb on + strand, within Rv3148 at 3514.495 kb on - strand, within Rv3148 at 3514.539 kb on - strand at 3514.763 kb on + strand, within Rv3149 at 3514.868 kb on - strand, within Rv3149 at 3514.948 kb on + strand, within Rv3149 at 3514.948 kb on + strand, within Rv3149 at 3514.949 kb on - strand, within Rv3149 at 3514.957 kb on + strand, within Rv3149 at 3514.957 kb on + strand, within Rv3149 at 3514.958 kb on - strand, within Rv3149 at 3514.958 kb on - strand, within Rv3149 at 3515.297 kb on + strand, within Rv3149 at 3515.491 kb on - strand at 3515.871 kb on + strand, within Rv3150 at 3515.952 kb on + strand, within Rv3150 at 3516.168 kb on + strand, within Rv3150 at 3516.168 kb on + strand, within Rv3150 at 3516.224 kb on + strand, within Rv3150 at 3516.453 kb on + strand, within Rv3150 at 3516.512 kb on + strand, within Rv3150 at 3516.519 kb on + strand, within Rv3150 at 3516.520 kb on - strand, within Rv3150 at 3517.596 kb on - strand, within Rv3151
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (C) remove 3,514,494 + Rv3148 0.87 -1.6 3,514,495 - Rv3148 0.87 -1.0 3,514,539 - -1.5 3,514,763 + Rv3149 0.14 -0.8 3,514,868 - Rv3149 0.28 -2.2 3,514,948 + Rv3149 0.38 -1.7 3,514,948 + Rv3149 0.38 -1.5 3,514,949 - Rv3149 0.38 -1.5 3,514,957 + Rv3149 0.40 -1.3 3,514,957 + Rv3149 0.40 -0.7 3,514,958 - Rv3149 0.40 -2.2 3,514,958 - Rv3149 0.40 -1.8 3,515,297 + Rv3149 0.84 -1.3 3,515,491 - -1.2 3,515,871 + Rv3150 0.34 -1.3 3,515,952 + Rv3150 0.40 -0.7 3,516,168 + Rv3150 0.57 -1.6 3,516,168 + Rv3150 0.57 -3.3 3,516,224 + Rv3150 0.61 -1.4 3,516,453 + Rv3150 0.78 -1.6 3,516,512 + Rv3150 0.82 -1.8 3,516,519 + Rv3150 0.83 +0.1 3,516,520 - Rv3150 0.83 -2.0 3,517,596 - Rv3151 0.35 -1.2
Or see this region's nucleotide sequence