Experiment: MoLS4 with L-Methionine & L-Threonine (10/10 mM) as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dmt and DVU3014 overlap by 13 nucleotides DVU3014 and DVU3015 overlap by 19 nucleotides DVU3015 and DVU3016 are separated by 114 nucleotides
DVU3013: dmt - glycosyl transferase, group 2 family protein (TIGR), at 3,126,886 to 3,128,592
dmt
DVU3014: DVU3014 - asparagine synthetase, glutamine-hydrolyzing (TIGR), at 3,128,580 to 3,130,673
DVU3014
DVU3015: DVU3015 - conserved domain protein (TIGR), at 3,130,655 to 3,131,221
DVU3015
DVU3016: DVU3016 - B12 binding domain protein/radical SAM domain protein (TIGR), at 3,131,336 to 3,132,781
DVU3016
Position (kb)
3128
3129
3130
3131 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3127.642 kb on + strand, within dmt at 3127.675 kb on + strand, within dmt at 3127.829 kb on + strand, within dmt at 3127.868 kb on - strand, within dmt at 3127.868 kb on - strand, within dmt at 3127.956 kb on + strand, within dmt at 3128.068 kb on + strand, within dmt at 3128.068 kb on + strand, within dmt at 3128.068 kb on + strand, within dmt at 3128.177 kb on - strand, within dmt at 3128.180 kb on + strand, within dmt at 3128.210 kb on - strand, within dmt at 3128.244 kb on + strand, within dmt at 3128.281 kb on - strand, within dmt at 3128.360 kb on + strand, within dmt at 3128.361 kb on + strand, within dmt at 3128.368 kb on - strand, within dmt at 3128.378 kb on - strand, within dmt at 3128.380 kb on + strand, within dmt at 3128.428 kb on + strand at 3128.457 kb on + strand at 3128.470 kb on + strand at 3128.483 kb on + strand at 3128.503 kb on + strand at 3128.516 kb on - strand at 3128.571 kb on - strand at 3128.631 kb on - strand at 3128.800 kb on - strand, within DVU3014 at 3128.833 kb on - strand, within DVU3014 at 3128.836 kb on + strand, within DVU3014 at 3128.839 kb on - strand, within DVU3014 at 3128.922 kb on - strand, within DVU3014 at 3128.935 kb on - strand, within DVU3014 at 3128.948 kb on + strand, within DVU3014 at 3129.038 kb on - strand, within DVU3014 at 3129.038 kb on - strand, within DVU3014 at 3129.038 kb on - strand, within DVU3014 at 3129.038 kb on - strand, within DVU3014 at 3129.038 kb on - strand, within DVU3014 at 3129.064 kb on - strand, within DVU3014 at 3129.080 kb on + strand, within DVU3014 at 3129.088 kb on - strand, within DVU3014 at 3129.137 kb on + strand, within DVU3014 at 3129.305 kb on - strand, within DVU3014 at 3129.343 kb on - strand, within DVU3014 at 3129.372 kb on - strand, within DVU3014 at 3129.408 kb on - strand, within DVU3014 at 3129.482 kb on - strand, within DVU3014 at 3129.529 kb on + strand, within DVU3014 at 3129.544 kb on + strand, within DVU3014 at 3129.611 kb on - strand, within DVU3014 at 3129.623 kb on - strand, within DVU3014 at 3129.651 kb on - strand, within DVU3014 at 3129.674 kb on + strand, within DVU3014 at 3129.791 kb on + strand, within DVU3014 at 3129.823 kb on - strand, within DVU3014 at 3129.902 kb on + strand, within DVU3014 at 3129.960 kb on - strand, within DVU3014 at 3129.960 kb on - strand, within DVU3014 at 3129.996 kb on + strand, within DVU3014 at 3130.049 kb on + strand, within DVU3014 at 3130.081 kb on + strand, within DVU3014 at 3130.131 kb on - strand, within DVU3014 at 3130.157 kb on - strand, within DVU3014 at 3130.157 kb on - strand, within DVU3014 at 3130.158 kb on - strand, within DVU3014 at 3130.166 kb on + strand, within DVU3014 at 3130.270 kb on - strand, within DVU3014 at 3130.312 kb on + strand, within DVU3014 at 3130.312 kb on + strand, within DVU3014 at 3130.351 kb on - strand, within DVU3014 at 3130.384 kb on - strand, within DVU3014 at 3130.392 kb on + strand, within DVU3014 at 3130.445 kb on + strand, within DVU3014 at 3130.562 kb on - strand at 3130.564 kb on - strand at 3130.566 kb on + strand at 3130.594 kb on - strand at 3130.594 kb on - strand at 3130.795 kb on + strand, within DVU3015 at 3130.820 kb on + strand, within DVU3015 at 3130.859 kb on - strand, within DVU3015 at 3130.871 kb on - strand, within DVU3015 at 3130.887 kb on + strand, within DVU3015 at 3130.922 kb on - strand, within DVU3015 at 3131.044 kb on + strand, within DVU3015 at 3131.044 kb on + strand, within DVU3015 at 3131.044 kb on + strand, within DVU3015 at 3131.053 kb on - strand, within DVU3015 at 3131.063 kb on + strand, within DVU3015 at 3131.079 kb on + strand, within DVU3015 at 3131.130 kb on - strand, within DVU3015 at 3131.210 kb on + strand at 3131.222 kb on + strand at 3131.222 kb on + strand at 3131.230 kb on - strand at 3131.268 kb on + strand at 3131.358 kb on + strand at 3131.358 kb on + strand at 3131.358 kb on + strand at 3131.358 kb on + strand at 3131.358 kb on + strand at 3131.358 kb on + strand at 3131.358 kb on + strand at 3131.358 kb on + strand at 3131.358 kb on + strand at 3131.358 kb on + strand at 3131.358 kb on + strand at 3131.358 kb on + strand at 3131.358 kb on + strand at 3131.366 kb on - strand at 3131.366 kb on - strand at 3131.366 kb on - strand at 3131.366 kb on - strand at 3131.366 kb on - strand at 3131.366 kb on - strand at 3131.456 kb on - strand at 3131.499 kb on + strand, within DVU3016 at 3131.544 kb on - strand, within DVU3016 at 3131.576 kb on + strand, within DVU3016 at 3131.600 kb on - strand, within DVU3016
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Methionine & L-Threonine (10/10 mM) as nutrient remove 3,127,642 + dmt DVU3013 0.44 -0.5 3,127,675 + dmt DVU3013 0.46 -1.0 3,127,829 + dmt DVU3013 0.55 -0.2 3,127,868 - dmt DVU3013 0.58 -0.8 3,127,868 - dmt DVU3013 0.58 -0.4 3,127,956 + dmt DVU3013 0.63 -0.2 3,128,068 + dmt DVU3013 0.69 +0.6 3,128,068 + dmt DVU3013 0.69 -0.8 3,128,068 + dmt DVU3013 0.69 -0.0 3,128,177 - dmt DVU3013 0.76 -1.2 3,128,180 + dmt DVU3013 0.76 -0.9 3,128,210 - dmt DVU3013 0.78 +0.5 3,128,244 + dmt DVU3013 0.80 -0.5 3,128,281 - dmt DVU3013 0.82 -0.9 3,128,360 + dmt DVU3013 0.86 -1.1 3,128,361 + dmt DVU3013 0.86 +1.6 3,128,368 - dmt DVU3013 0.87 -0.2 3,128,378 - dmt DVU3013 0.87 -0.5 3,128,380 + dmt DVU3013 0.88 -0.1 3,128,428 + -0.9 3,128,457 + -0.4 3,128,470 + -0.0 3,128,483 + -0.8 3,128,503 + -0.4 3,128,516 - +0.5 3,128,571 - -0.3 3,128,631 - -0.2 3,128,800 - DVU3014 0.11 -0.8 3,128,833 - DVU3014 0.12 -1.5 3,128,836 + DVU3014 0.12 -0.9 3,128,839 - DVU3014 0.12 +0.0 3,128,922 - DVU3014 0.16 -0.4 3,128,935 - DVU3014 0.17 -1.9 3,128,948 + DVU3014 0.18 -0.7 3,129,038 - DVU3014 0.22 +0.2 3,129,038 - DVU3014 0.22 -0.1 3,129,038 - DVU3014 0.22 -1.4 3,129,038 - DVU3014 0.22 +0.1 3,129,038 - DVU3014 0.22 -1.2 3,129,064 - DVU3014 0.23 -0.4 3,129,080 + DVU3014 0.24 -0.4 3,129,088 - DVU3014 0.24 -1.3 3,129,137 + DVU3014 0.27 -1.0 3,129,305 - DVU3014 0.35 -1.3 3,129,343 - DVU3014 0.36 -1.0 3,129,372 - DVU3014 0.38 -1.1 3,129,408 - DVU3014 0.40 -2.0 3,129,482 - DVU3014 0.43 +0.1 3,129,529 + DVU3014 0.45 +0.8 3,129,544 + DVU3014 0.46 -0.4 3,129,611 - DVU3014 0.49 -1.4 3,129,623 - DVU3014 0.50 -1.6 3,129,651 - DVU3014 0.51 -0.4 3,129,674 + DVU3014 0.52 +1.2 3,129,791 + DVU3014 0.58 -1.4 3,129,823 - DVU3014 0.59 -0.2 3,129,902 + DVU3014 0.63 -0.7 3,129,960 - DVU3014 0.66 +0.4 3,129,960 - DVU3014 0.66 -1.6 3,129,996 + DVU3014 0.68 +0.2 3,130,049 + DVU3014 0.70 -1.0 3,130,081 + DVU3014 0.72 -1.6 3,130,131 - DVU3014 0.74 -1.4 3,130,157 - DVU3014 0.75 -0.5 3,130,157 - DVU3014 0.75 -1.3 3,130,158 - DVU3014 0.75 -0.4 3,130,166 + DVU3014 0.76 -0.4 3,130,270 - DVU3014 0.81 -1.2 3,130,312 + DVU3014 0.83 +0.3 3,130,312 + DVU3014 0.83 +0.1 3,130,351 - DVU3014 0.85 +0.2 3,130,384 - DVU3014 0.86 -1.4 3,130,392 + DVU3014 0.87 +0.3 3,130,445 + DVU3014 0.89 +0.4 3,130,562 - +1.8 3,130,564 - -0.0 3,130,566 + -0.4 3,130,594 - +0.3 3,130,594 - -0.5 3,130,795 + DVU3015 0.25 -0.2 3,130,820 + DVU3015 0.29 -0.6 3,130,859 - DVU3015 0.36 -1.0 3,130,871 - DVU3015 0.38 +1.2 3,130,887 + DVU3015 0.41 -0.5 3,130,922 - DVU3015 0.47 -0.7 3,131,044 + DVU3015 0.69 -0.3 3,131,044 + DVU3015 0.69 +0.6 3,131,044 + DVU3015 0.69 -0.1 3,131,053 - DVU3015 0.70 -0.8 3,131,063 + DVU3015 0.72 +0.0 3,131,079 + DVU3015 0.75 -0.2 3,131,130 - DVU3015 0.84 -0.2 3,131,210 + +0.0 3,131,222 + -0.3 3,131,222 + -0.1 3,131,230 - +0.0 3,131,268 + -0.6 3,131,358 + -0.7 3,131,358 + -0.3 3,131,358 + -1.5 3,131,358 + -0.7 3,131,358 + +0.8 3,131,358 + -0.7 3,131,358 + -0.1 3,131,358 + +0.4 3,131,358 + +0.1 3,131,358 + -1.0 3,131,358 + +0.1 3,131,358 + +1.8 3,131,358 + -0.7 3,131,366 - -1.2 3,131,366 - -0.3 3,131,366 - -0.3 3,131,366 - +0.2 3,131,366 - +0.1 3,131,366 - -1.4 3,131,456 - -0.8 3,131,499 + DVU3016 0.11 -0.8 3,131,544 - DVU3016 0.14 -0.0 3,131,576 + DVU3016 0.17 -0.1 3,131,600 - DVU3016 0.18 +0.2
Or see this region's nucleotide sequence