Experiment: MoLS4 with L-Methionine (10mM) as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metE and DVU3372 are separated by 324 nucleotides DVU3372 and ilvD are separated by 80 nucleotides ilvD and DVU3374 are separated by 31 nucleotides DVU3374 and ftsE are separated by 41 nucleotides
DVU3371: metE - 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase (TIGR), at 3,542,097 to 3,544,454
metE
DVU3372: DVU3372 - hypothetical protein (TIGR), at 3,544,779 to 3,544,910
DVU3372
DVU3373: ilvD - dihydroxy-acid dehydratase (TIGR), at 3,544,991 to 3,546,655
ilvD
DVU3374: DVU3374 - permease, putative (TIGR), at 3,546,687 to 3,547,520
DVU3374
DVU3375: ftsE - cell division ATP-binding protein FtsE, putative (TIGR), at 3,547,562 to 3,548,278
ftsE
Position (kb)
3544
3545
3546
3547 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 3544.044 kb on + strand, within metE at 3544.119 kb on + strand, within metE at 3544.172 kb on - strand, within metE at 3544.172 kb on - strand, within metE at 3544.172 kb on - strand, within metE at 3544.177 kb on - strand, within metE at 3544.195 kb on + strand, within metE at 3544.204 kb on + strand, within metE at 3544.232 kb on + strand at 3544.244 kb on + strand at 3544.315 kb on + strand at 3544.316 kb on - strand at 3544.318 kb on - strand at 3544.327 kb on + strand at 3544.327 kb on + strand at 3544.335 kb on - strand at 3544.335 kb on - strand at 3544.396 kb on + strand at 3544.471 kb on + strand at 3544.471 kb on + strand at 3544.471 kb on + strand at 3544.471 kb on + strand at 3544.473 kb on + strand at 3544.504 kb on + strand at 3544.519 kb on + strand at 3544.527 kb on - strand at 3544.557 kb on + strand at 3544.564 kb on + strand at 3544.565 kb on - strand at 3544.565 kb on - strand at 3544.565 kb on - strand at 3544.575 kb on + strand at 3544.708 kb on + strand at 3544.738 kb on + strand at 3544.743 kb on + strand at 3544.837 kb on + strand, within DVU3372 at 3544.870 kb on - strand, within DVU3372 at 3544.871 kb on + strand, within DVU3372 at 3544.872 kb on + strand, within DVU3372 at 3544.904 kb on + strand at 3544.956 kb on - strand at 3544.994 kb on + strand at 3545.026 kb on - strand at 3545.125 kb on + strand at 3545.133 kb on - strand at 3545.133 kb on - strand at 3545.168 kb on + strand, within ilvD at 3545.176 kb on - strand, within ilvD at 3545.212 kb on + strand, within ilvD at 3545.238 kb on - strand, within ilvD at 3545.254 kb on - strand, within ilvD at 3545.275 kb on + strand, within ilvD at 3545.296 kb on - strand, within ilvD at 3545.343 kb on + strand, within ilvD at 3545.371 kb on - strand, within ilvD at 3545.494 kb on + strand, within ilvD at 3545.557 kb on - strand, within ilvD at 3545.662 kb on + strand, within ilvD at 3545.662 kb on + strand, within ilvD at 3545.662 kb on + strand, within ilvD at 3545.662 kb on + strand, within ilvD at 3545.662 kb on + strand, within ilvD at 3545.667 kb on + strand, within ilvD at 3545.670 kb on - strand, within ilvD at 3545.670 kb on - strand, within ilvD at 3545.670 kb on - strand, within ilvD at 3545.670 kb on - strand, within ilvD at 3545.682 kb on + strand, within ilvD at 3545.741 kb on + strand, within ilvD at 3545.766 kb on - strand, within ilvD at 3545.837 kb on + strand, within ilvD at 3545.837 kb on + strand, within ilvD at 3545.845 kb on - strand, within ilvD at 3545.869 kb on - strand, within ilvD at 3545.923 kb on + strand, within ilvD at 3546.025 kb on + strand, within ilvD at 3546.033 kb on - strand, within ilvD at 3546.108 kb on - strand, within ilvD at 3546.118 kb on - strand, within ilvD at 3546.188 kb on - strand, within ilvD at 3546.205 kb on + strand, within ilvD at 3546.261 kb on - strand, within ilvD at 3546.270 kb on + strand, within ilvD at 3546.324 kb on + strand, within ilvD at 3546.332 kb on - strand, within ilvD at 3546.370 kb on + strand, within ilvD at 3546.468 kb on - strand, within ilvD at 3546.475 kb on + strand, within ilvD at 3546.488 kb on + strand, within ilvD at 3546.566 kb on + strand at 3546.571 kb on + strand at 3546.571 kb on + strand at 3546.581 kb on + strand at 3546.586 kb on + strand at 3546.586 kb on + strand at 3546.728 kb on - strand at 3546.808 kb on + strand, within DVU3374 at 3546.821 kb on - strand, within DVU3374 at 3546.876 kb on + strand, within DVU3374 at 3546.903 kb on + strand, within DVU3374 at 3546.911 kb on + strand, within DVU3374 at 3546.924 kb on - strand, within DVU3374 at 3546.983 kb on + strand, within DVU3374 at 3547.008 kb on - strand, within DVU3374 at 3547.010 kb on + strand, within DVU3374 at 3547.031 kb on + strand, within DVU3374 at 3547.050 kb on + strand, within DVU3374 at 3547.058 kb on - strand, within DVU3374 at 3547.072 kb on + strand, within DVU3374 at 3547.102 kb on - strand, within DVU3374 at 3547.102 kb on - strand, within DVU3374 at 3547.117 kb on - strand, within DVU3374 at 3547.117 kb on - strand, within DVU3374 at 3547.296 kb on - strand, within DVU3374 at 3547.313 kb on - strand, within DVU3374 at 3547.328 kb on + strand, within DVU3374 at 3547.328 kb on - strand, within DVU3374 at 3547.347 kb on + strand, within DVU3374 at 3547.382 kb on - strand, within DVU3374 at 3547.382 kb on - strand, within DVU3374 at 3547.383 kb on - strand, within DVU3374 at 3547.431 kb on + strand, within DVU3374 at 3547.431 kb on + strand, within DVU3374 at 3547.439 kb on - strand at 3547.439 kb on - strand at 3547.461 kb on - strand at 3547.487 kb on + strand at 3547.517 kb on - strand at 3547.517 kb on - strand at 3547.545 kb on + strand at 3547.545 kb on + strand at 3547.545 kb on + strand at 3547.569 kb on + strand at 3547.625 kb on + strand at 3547.643 kb on - strand, within ftsE at 3547.644 kb on + strand, within ftsE
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Methionine (10mM) as nutrient remove 3,544,044 + metE DVU3371 0.83 +0.0 3,544,119 + metE DVU3371 0.86 -0.7 3,544,172 - metE DVU3371 0.88 -0.9 3,544,172 - metE DVU3371 0.88 -0.2 3,544,172 - metE DVU3371 0.88 +0.9 3,544,177 - metE DVU3371 0.88 +0.2 3,544,195 + metE DVU3371 0.89 -0.1 3,544,204 + metE DVU3371 0.89 -1.2 3,544,232 + +0.3 3,544,244 + +1.8 3,544,315 + +1.0 3,544,316 - -1.3 3,544,318 - -0.2 3,544,327 + -0.0 3,544,327 + -1.0 3,544,335 - -1.0 3,544,335 - -0.4 3,544,396 + -0.8 3,544,471 + +1.3 3,544,471 + +0.3 3,544,471 + -0.1 3,544,471 + -0.8 3,544,473 + +0.4 3,544,504 + +0.1 3,544,519 + -1.1 3,544,527 - -0.4 3,544,557 + -0.4 3,544,564 + +0.7 3,544,565 - -1.3 3,544,565 - -0.2 3,544,565 - -0.7 3,544,575 + -0.9 3,544,708 + +1.4 3,544,738 + -0.0 3,544,743 + -0.3 3,544,837 + DVU3372 0.44 -0.8 3,544,870 - DVU3372 0.69 -0.5 3,544,871 + DVU3372 0.70 +0.1 3,544,872 + DVU3372 0.70 -0.2 3,544,904 + -0.1 3,544,956 - +0.3 3,544,994 + -4.6 3,545,026 - -5.7 3,545,125 + -3.5 3,545,133 - -2.9 3,545,133 - -1.2 3,545,168 + ilvD DVU3373 0.11 -4.3 3,545,176 - ilvD DVU3373 0.11 -3.7 3,545,212 + ilvD DVU3373 0.13 -3.5 3,545,238 - ilvD DVU3373 0.15 -2.9 3,545,254 - ilvD DVU3373 0.16 -2.5 3,545,275 + ilvD DVU3373 0.17 -0.8 3,545,296 - ilvD DVU3373 0.18 -3.0 3,545,343 + ilvD DVU3373 0.21 -5.1 3,545,371 - ilvD DVU3373 0.23 -2.6 3,545,494 + ilvD DVU3373 0.30 -1.1 3,545,557 - ilvD DVU3373 0.34 -4.5 3,545,662 + ilvD DVU3373 0.40 -1.6 3,545,662 + ilvD DVU3373 0.40 -1.3 3,545,662 + ilvD DVU3373 0.40 -6.2 3,545,662 + ilvD DVU3373 0.40 -4.8 3,545,662 + ilvD DVU3373 0.40 -1.4 3,545,667 + ilvD DVU3373 0.41 -3.0 3,545,670 - ilvD DVU3373 0.41 -4.9 3,545,670 - ilvD DVU3373 0.41 -1.5 3,545,670 - ilvD DVU3373 0.41 -2.3 3,545,670 - ilvD DVU3373 0.41 -4.3 3,545,682 + ilvD DVU3373 0.42 -3.7 3,545,741 + ilvD DVU3373 0.45 -4.1 3,545,766 - ilvD DVU3373 0.47 -3.8 3,545,837 + ilvD DVU3373 0.51 -3.1 3,545,837 + ilvD DVU3373 0.51 -5.0 3,545,845 - ilvD DVU3373 0.51 -1.9 3,545,869 - ilvD DVU3373 0.53 -5.7 3,545,923 + ilvD DVU3373 0.56 -6.0 3,546,025 + ilvD DVU3373 0.62 -3.9 3,546,033 - ilvD DVU3373 0.63 -4.7 3,546,108 - ilvD DVU3373 0.67 -0.4 3,546,118 - ilvD DVU3373 0.68 -3.7 3,546,188 - ilvD DVU3373 0.72 -3.3 3,546,205 + ilvD DVU3373 0.73 -2.5 3,546,261 - ilvD DVU3373 0.76 -3.9 3,546,270 + ilvD DVU3373 0.77 -3.7 3,546,324 + ilvD DVU3373 0.80 -4.1 3,546,332 - ilvD DVU3373 0.81 -2.4 3,546,370 + ilvD DVU3373 0.83 -4.2 3,546,468 - ilvD DVU3373 0.89 -5.1 3,546,475 + ilvD DVU3373 0.89 -3.2 3,546,488 + ilvD DVU3373 0.90 -5.8 3,546,566 + -3.3 3,546,571 + -4.3 3,546,571 + -1.5 3,546,581 + -1.4 3,546,586 + -4.5 3,546,586 + -1.3 3,546,728 - -1.0 3,546,808 + DVU3374 0.15 +0.7 3,546,821 - DVU3374 0.16 -0.0 3,546,876 + DVU3374 0.23 -0.2 3,546,903 + DVU3374 0.26 -0.9 3,546,911 + DVU3374 0.27 -0.3 3,546,924 - DVU3374 0.28 -0.0 3,546,983 + DVU3374 0.35 -0.4 3,547,008 - DVU3374 0.38 -0.4 3,547,010 + DVU3374 0.39 +0.0 3,547,031 + DVU3374 0.41 +0.8 3,547,050 + DVU3374 0.44 -0.2 3,547,058 - DVU3374 0.44 +0.6 3,547,072 + DVU3374 0.46 -0.9 3,547,102 - DVU3374 0.50 -0.4 3,547,102 - DVU3374 0.50 -0.1 3,547,117 - DVU3374 0.52 -0.1 3,547,117 - DVU3374 0.52 -0.0 3,547,296 - DVU3374 0.73 +0.3 3,547,313 - DVU3374 0.75 -0.8 3,547,328 + DVU3374 0.77 -0.5 3,547,328 - DVU3374 0.77 -0.1 3,547,347 + DVU3374 0.79 -0.3 3,547,382 - DVU3374 0.83 -0.2 3,547,382 - DVU3374 0.83 +0.3 3,547,383 - DVU3374 0.83 -0.3 3,547,431 + DVU3374 0.89 +0.0 3,547,431 + DVU3374 0.89 +0.1 3,547,439 - +0.5 3,547,439 - -0.3 3,547,461 - -0.1 3,547,487 + -0.6 3,547,517 - -0.4 3,547,517 - +1.4 3,547,545 + -1.8 3,547,545 + +0.7 3,547,545 + -0.5 3,547,569 + +0.4 3,547,625 + -1.8 3,547,643 - ftsE DVU3375 0.11 -0.8 3,547,644 + ftsE DVU3375 0.11 -0.2
Or see this region's nucleotide sequence