Experiment: MoLS4 with L-Methionine (10mM) as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2287 and DVU2288 are separated by 12 nucleotides DVU2288 and b2488 are separated by 1 nucleotides b2488 and DVU2290 overlap by 4 nucleotides DVU2290 and DVU2291 overlap by 4 nucleotides
DVU2287: DVU2287 - hydrogenase, CooK subunit, selenocysteine-containing, putative (TIGR), at 2,381,054 to 2,382,019
DVU2287
DVU2288: DVU2288 - hydrogenase, CooL subunit, putative (TIGR), at 2,382,032 to 2,382,466
DVU2288
DVU2289: b2488 - hydrogenase, CooX subunit, putative (TIGR), at 2,382,468 to 2,383,103
b2488
DVU2290: DVU2290 - hydrogenase, CooU subunit, putative (TIGR), at 2,383,100 to 2,383,645
DVU2290
DVU2291: DVU2291 - carbon monoxide-induced hydrogenase CooH, putative (TIGR), at 2,383,642 to 2,384,742
DVU2291
Position (kb)
2382
2383
2384 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2381.490 kb on - strand, within DVU2287 at 2381.605 kb on + strand, within DVU2287 at 2382.243 kb on - strand, within DVU2288 at 2382.750 kb on - strand, within b2488 at 2382.926 kb on + strand, within b2488 at 2383.041 kb on + strand at 2383.041 kb on + strand at 2383.041 kb on + strand at 2383.116 kb on + strand at 2383.243 kb on + strand, within DVU2290 at 2383.365 kb on + strand, within DVU2290 at 2383.639 kb on + strand at 2383.775 kb on + strand, within DVU2291 at 2383.819 kb on - strand, within DVU2291 at 2384.062 kb on + strand, within DVU2291
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Methionine (10mM) as nutrient remove 2,381,490 - DVU2287 0.45 -1.3 2,381,605 + DVU2287 0.57 -0.2 2,382,243 - DVU2288 0.49 +0.5 2,382,750 - b2488 DVU2289 0.44 -0.1 2,382,926 + b2488 DVU2289 0.72 -1.0 2,383,041 + -0.0 2,383,041 + +0.7 2,383,041 + -0.7 2,383,116 + -2.1 2,383,243 + DVU2290 0.26 -0.5 2,383,365 + DVU2290 0.49 -0.2 2,383,639 + -0.4 2,383,775 + DVU2291 0.12 -0.1 2,383,819 - DVU2291 0.16 +1.9 2,384,062 + DVU2291 0.38 -0.3
Or see this region's nucleotide sequence