Experiment: MoLS4 with L-Methionine (10mM) as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cckA and DVU3217 are separated by 685 nucleotides DVU3217 and pncA are separated by 251 nucleotides pncA and DVU3219 are separated by 35 nucleotides
DVU3216: cckA - sensor histidine kinase (TIGR), at 3,383,335 to 3,385,032
cckA
DVU3217: DVU3217 - hypothetical protein (TIGR), at 3,385,718 to 3,386,146
DVU3217
DVU3218: pncA - pyrazinamidase/nicotinamidase (TIGR), at 3,386,398 to 3,387,018
pncA
DVU3219: DVU3219 - hypothetical protein (TIGR), at 3,387,054 to 3,388,256
DVU3219
Position (kb)
3385
3386
3387 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3384.850 kb on + strand, within cckA at 3384.860 kb on - strand, within cckA at 3384.911 kb on - strand at 3385.057 kb on - strand at 3385.199 kb on - strand at 3385.213 kb on - strand at 3385.239 kb on + strand at 3385.243 kb on - strand at 3385.249 kb on + strand at 3385.249 kb on + strand at 3385.257 kb on - strand at 3385.257 kb on - strand at 3385.257 kb on - strand at 3385.257 kb on - strand at 3385.257 kb on - strand at 3385.257 kb on - strand at 3385.257 kb on - strand at 3385.288 kb on + strand at 3385.371 kb on - strand at 3385.443 kb on + strand at 3385.461 kb on - strand at 3385.520 kb on + strand at 3385.534 kb on + strand at 3385.534 kb on + strand at 3385.551 kb on + strand at 3385.716 kb on + strand at 3385.724 kb on - strand at 3385.776 kb on + strand, within DVU3217 at 3385.826 kb on - strand, within DVU3217 at 3385.874 kb on - strand, within DVU3217 at 3385.921 kb on + strand, within DVU3217 at 3385.921 kb on - strand, within DVU3217 at 3385.956 kb on - strand, within DVU3217 at 3385.987 kb on + strand, within DVU3217 at 3385.995 kb on - strand, within DVU3217 at 3386.068 kb on - strand, within DVU3217 at 3386.223 kb on + strand at 3386.225 kb on - strand at 3386.231 kb on - strand at 3386.231 kb on - strand at 3386.353 kb on - strand at 3386.390 kb on + strand at 3386.390 kb on + strand at 3386.402 kb on + strand at 3386.403 kb on - strand at 3386.403 kb on - strand at 3386.586 kb on - strand, within pncA at 3386.608 kb on + strand, within pncA at 3386.639 kb on - strand, within pncA at 3386.649 kb on - strand, within pncA at 3386.695 kb on + strand, within pncA at 3386.695 kb on + strand, within pncA at 3386.698 kb on - strand, within pncA at 3386.712 kb on + strand, within pncA at 3386.846 kb on + strand, within pncA at 3386.856 kb on + strand, within pncA at 3386.902 kb on - strand, within pncA at 3386.902 kb on - strand, within pncA at 3386.933 kb on + strand, within pncA at 3386.941 kb on - strand, within pncA at 3386.948 kb on + strand, within pncA at 3386.973 kb on + strand at 3387.010 kb on - strand at 3387.022 kb on - strand at 3387.046 kb on + strand at 3387.054 kb on - strand at 3387.071 kb on + strand at 3387.079 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Methionine (10mM) as nutrient remove 3,384,850 + cckA DVU3216 0.89 -1.1 3,384,860 - cckA DVU3216 0.90 -0.2 3,384,911 - +0.0 3,385,057 - -0.7 3,385,199 - -0.9 3,385,213 - +1.2 3,385,239 + +0.1 3,385,243 - -1.3 3,385,249 + -1.0 3,385,249 + -1.1 3,385,257 - +0.1 3,385,257 - -0.8 3,385,257 - -0.7 3,385,257 - +0.9 3,385,257 - -1.8 3,385,257 - -0.1 3,385,257 - -0.8 3,385,288 + -1.1 3,385,371 - -0.1 3,385,443 + -1.9 3,385,461 - +1.2 3,385,520 + -0.5 3,385,534 + -0.1 3,385,534 + -0.6 3,385,551 + -0.2 3,385,716 + -0.4 3,385,724 - +0.2 3,385,776 + DVU3217 0.14 -0.1 3,385,826 - DVU3217 0.25 -2.1 3,385,874 - DVU3217 0.36 -0.4 3,385,921 + DVU3217 0.47 -0.9 3,385,921 - DVU3217 0.47 -0.7 3,385,956 - DVU3217 0.55 +0.3 3,385,987 + DVU3217 0.63 -0.6 3,385,995 - DVU3217 0.65 -1.9 3,386,068 - DVU3217 0.82 +0.3 3,386,223 + -0.0 3,386,225 - -0.7 3,386,231 - +0.6 3,386,231 - -0.9 3,386,353 - -0.1 3,386,390 + -0.9 3,386,390 + -1.4 3,386,402 + +0.1 3,386,403 - +0.5 3,386,403 - +0.0 3,386,586 - pncA DVU3218 0.30 -0.5 3,386,608 + pncA DVU3218 0.34 +0.2 3,386,639 - pncA DVU3218 0.39 +0.2 3,386,649 - pncA DVU3218 0.40 +0.1 3,386,695 + pncA DVU3218 0.48 -0.0 3,386,695 + pncA DVU3218 0.48 -0.3 3,386,698 - pncA DVU3218 0.48 +0.9 3,386,712 + pncA DVU3218 0.51 -0.1 3,386,846 + pncA DVU3218 0.72 +1.4 3,386,856 + pncA DVU3218 0.74 -0.6 3,386,902 - pncA DVU3218 0.81 +1.3 3,386,902 - pncA DVU3218 0.81 -0.6 3,386,933 + pncA DVU3218 0.86 -0.8 3,386,941 - pncA DVU3218 0.87 +0.3 3,386,948 + pncA DVU3218 0.89 +0.0 3,386,973 + +0.6 3,387,010 - +2.0 3,387,022 - -1.9 3,387,046 + -0.2 3,387,054 - -0.4 3,387,071 + +0.2 3,387,079 - +0.3
Or see this region's nucleotide sequence